Click here to switch to the map view.

The map label for this gene is eno

Identifier: 78778596

GI number: 78778596

Start: 202018

End: 203310

Strand: Direct

Name: eno

Synonym: PMT9312_0210

Alternate gene names: 78778596

Gene position: 202018-203310 (Clockwise)

Preceding gene: 78778585

Following gene: 78778599

Centisome position: 11.82

GC content: 36.19

Gene sequence:

>1293_bases
GTGAAAGAAACTATTGATTTTCTTATTGATACTATTGAAGCAAGGCAAGTCCTTGATTCAAGAGGCAATCCGACTGTAGA
GGCAGAAGTATTTTTGGAATGTGGGGCAAGTGGTAGAGCAATTGTGCCTAGCGGAGCCAGCACTGGTGCTCATGAGGCGC
ATGAATTAAGAGATGGCGGTTCAAAATATATGGGGAAAGGTGTTTTAAACGCTGTTAATAAAATTCATGAAACAATATCG
CCTGCTTTATGTGGTTTGTCAGCTTTAGATCAAACTACAGTAGATAAATTAATGATTGAAATTGATGGGACTTTTAATAA
GTCTAACCTTGGAGCAAATTCTATCCTTGCAGTAAGTCTTGCAACTGCTAGAGCATCAGCAAATGCTTTAGACGTGCCCC
TTTATAGGTATCTTGGAGATCCATTATCCAATCTTCTTCCAGTCCCATTGATGAATGTAATAAATGGTGGTGCTCATGCT
CCAAATAGTCTTGATTTTCAGGAATTTATGCTTGTCCCACATGGAGTTCAAAATTTTAGTGAATCATTAAGAATGGGTAC
TGAAATTTTTCACTCATTAAAATCATTACTTGATAAAAAAGGTCTATCTACTGCTGTAGGCGATGAGGGAGGATTTGCAC
CTAATTTGTCATCCAGCGAAGAAGCAGGGGACTTATTATTAGAAGCAATTCAAAAAGCCGGATTTATTCCTGGTGAGCAA
GTATCTTTAGCTTTAGATGCTGCTAGTACTGAATTTTATAGTGATGGTATTTATAAATATGAAGGAAAAAGTTTAAATAG
TTCTGAAATGATTTCATATCTTTCAAGATTAGTTTCTAATTATCCAATAGTTTCAATAGAGGACGGTTTAGCAGAGGATG
ATTGGGAGGGTTGGTCAGAATTAAATAAAGAATTAGGAAATAAAGTTCAGCTCGTAGGTGATGATTTATTCGTTACTAAT
ACAGAAAGGTTAAGGAAAGGGATTATGGAAAAATCTGCTAATTCAATCCTAATAAAGGTAAATCAAATTGGAACATTAAC
TGAAACTTTGGAAGCTATTGAGTTAGCCAAAACTTCTGGTTTTACAAGTGTGATAAGTCATAGAAGTGGTGAGACTGAAG
ATACAACAATTGCTGATTTATCTGTCGCCACAAGATCTGGTCAGATCAAGACCGGCTCTTTGAGTAGAAGTGAAAGGATT
GCTAAATATAATAGGCTTTTAAAAATTGAGGAGGAGTTGGGAAATCAAGCAAGATTCGCTGGAGCTTTAGGTTTAGGACC
CAAAAATATATAG

Upstream 100 bases:

>100_bases
TTCTGAAATCAAAAAAAATTATATTTGATTAACATATATATTTCAGATATTAGCCAAATAGTTCAGCTATTAATAGGATT
TAAATAGTAATAATTAAATT

Downstream 100 bases:

>100_bases
TTTTTCTAGCGCTTAAGTTTTTCTAAACGTGAGCCTTTACTCATAATTAATTGGCATTTTATCCAATCAATACTTATTGG
TACTGCAAAAAATAATGGTA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MKETIDFLIDTIEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGGSKYMGKGVLNAVNKIHETIS
PALCGLSALDQTTVDKLMIEIDGTFNKSNLGANSILAVSLATARASANALDVPLYRYLGDPLSNLLPVPLMNVINGGAHA
PNSLDFQEFMLVPHGVQNFSESLRMGTEIFHSLKSLLDKKGLSTAVGDEGGFAPNLSSSEEAGDLLLEAIQKAGFIPGEQ
VSLALDAASTEFYSDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSELNKELGNKVQLVGDDLFVTN
TERLRKGIMEKSANSILIKVNQIGTLTETLEAIELAKTSGFTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERI
AKYNRLLKIEEELGNQARFAGALGLGPKNI

Sequences:

>Translated_430_residues
MKETIDFLIDTIEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGGSKYMGKGVLNAVNKIHETIS
PALCGLSALDQTTVDKLMIEIDGTFNKSNLGANSILAVSLATARASANALDVPLYRYLGDPLSNLLPVPLMNVINGGAHA
PNSLDFQEFMLVPHGVQNFSESLRMGTEIFHSLKSLLDKKGLSTAVGDEGGFAPNLSSSEEAGDLLLEAIQKAGFIPGEQ
VSLALDAASTEFYSDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSELNKELGNKVQLVGDDLFVTN
TERLRKGIMEKSANSILIKVNQIGTLTETLEAIELAKTSGFTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERI
AKYNRLLKIEEELGNQARFAGALGLGPKNI
>Mature_430_residues
MKETIDFLIDTIEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGGSKYMGKGVLNAVNKIHETIS
PALCGLSALDQTTVDKLMIEIDGTFNKSNLGANSILAVSLATARASANALDVPLYRYLGDPLSNLLPVPLMNVINGGAHA
PNSLDFQEFMLVPHGVQNFSESLRMGTEIFHSLKSLLDKKGLSTAVGDEGGFAPNLSSSEEAGDLLLEAIQKAGFIPGEQ
VSLALDAASTEFYSDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSELNKELGNKVQLVGDDLFVTN
TERLRKGIMEKSANSILIKVNQIGTLTETLEAIELAKTSGFTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERI
AKYNRLLKIEEELGNQARFAGALGLGPKNI

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=50.9345794392523, Blast_Score=421, Evalue=1e-117,
Organism=Homo sapiens, GI4503571, Length=425, Percent_Identity=50.8235294117647, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897477, Length=435, Percent_Identity=50.1149425287356, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897469, Length=435, Percent_Identity=50.1149425287356, Blast_Score=402, Evalue=1e-112,
Organism=Homo sapiens, GI301897479, Length=433, Percent_Identity=44.3418013856813, Blast_Score=340, Evalue=2e-93,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=26.865671641791, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=26.865671641791, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=26.865671641791, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI1789141, Length=421, Percent_Identity=56.7695961995249, Blast_Score=463, Evalue=1e-132,
Organism=Caenorhabditis elegans, GI17536383, Length=429, Percent_Identity=50.3496503496504, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71995829, Length=429, Percent_Identity=50.3496503496504, Blast_Score=409, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32563855, Length=190, Percent_Identity=46.8421052631579, Blast_Score=185, Evalue=4e-47,
Organism=Saccharomyces cerevisiae, GI6321693, Length=432, Percent_Identity=51.3888888888889, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=50.1154734411085, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=50.1154734411085, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.8845265588915, Blast_Score=394, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6321968, Length=432, Percent_Identity=50.6944444444444, Blast_Score=375, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580918, Length=429, Percent_Identity=49.1841491841492, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=429, Percent_Identity=49.1841491841492, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=429, Percent_Identity=49.1841491841492, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=429, Percent_Identity=49.1841491841492, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=429, Percent_Identity=49.1841491841492, Blast_Score=391, Evalue=1e-109,
Organism=Drosophila melanogaster, GI17137654, Length=429, Percent_Identity=49.1841491841492, Blast_Score=391, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 46071; Mature: 46071

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETIDFLIDTIEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGG
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHCCC
SKYMGKGVLNAVNKIHETISPALCGLSALDQTTVDKLMIEIDGTFNKSNLGANSILAVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEE
ATARASANALDVPLYRYLGDPLSNLLPVPLMNVINGGAHAPNSLDFQEFMLVPHGVQNFS
HHHHCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
ESLRMGTEIFHSLKSLLDKKGLSTAVGDEGGFAPNLSSSEEAGDLLLEAIQKAGFIPGEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VSLALDAASTEFYSDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSE
EEEEEECCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
LNKELGNKVQLVGDDLFVTNTERLRKGIMEKSANSILIKVNQIGTLTETLEAIELAKTSG
HHHHHCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
FTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERIAKYNRLLKIEEELGNQARFA
HHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
GALGLGPKNI
HHCCCCCCCC
>Mature Secondary Structure
MKETIDFLIDTIEARQVLDSRGNPTVEAEVFLECGASGRAIVPSGASTGAHEAHELRDGG
CCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCEEECCCCCCCCHHHHHHHCCC
SKYMGKGVLNAVNKIHETISPALCGLSALDQTTVDKLMIEIDGTFNKSNLGANSILAVSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEE
ATARASANALDVPLYRYLGDPLSNLLPVPLMNVINGGAHAPNSLDFQEFMLVPHGVQNFS
HHHHCCCCCCCCHHHHHHCCHHHHCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
ESLRMGTEIFHSLKSLLDKKGLSTAVGDEGGFAPNLSSSEEAGDLLLEAIQKAGFIPGEQ
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
VSLALDAASTEFYSDGIYKYEGKSLNSSEMISYLSRLVSNYPIVSIEDGLAEDDWEGWSE
EEEEEECCCHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHH
LNKELGNKVQLVGDDLFVTNTERLRKGIMEKSANSILIKVNQIGTLTETLEAIELAKTSG
HHHHHCCEEEEEECCEEEECHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
FTSVISHRSGETEDTTIADLSVATRSGQIKTGSLSRSERIAKYNRLLKIEEELGNQARFA
HHHHHHCCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHH
GALGLGPKNI
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA