Click here to switch to the map view.

The map label for this gene is clpB2 [H]

Identifier: 78778595

GI number: 78778595

Start: 198996

End: 201752

Strand: Reverse

Name: clpB2 [H]

Synonym: PMT9312_0209

Alternate gene names: 78778595

Gene position: 201752-198996 (Counterclockwise)

Preceding gene: 78778597

Following gene: 78778594

Centisome position: 11.8

GC content: 30.72

Gene sequence:

>2757_bases
ATGAGAGAAACCCTTACATCCAGTCCTGAACTTTTTAGCGATATAAGTTGGAATCTTCTTCTATTAGGTGAAGAAACCGC
AAAAAAATGGGATCATAGCGAATTTAATATTGAACACATAATTCATACATTGTTCTCATCAAGAGAATTCTTTGCTTTCA
TTGAGAAATTATCAATCGACCAAGATACAGTTTTAGATATAACAGAAGATTTTTTAGAAGAGACACCAACAAATGAGTCA
GATATTTTTACTATCGGAGAAGATTTAGAAATTTTATTAGATAATGCAAATCAAATTAAAATTCAATGGGGATCGAGATT
AATAGAAATCCCTCATTTACTAATTGCTCTTGGAAGAGATTTAAGAATTGGTAATTATGTTTTTGAAGAAGGCAATCTTT
CAATGGAAAAATTAGAGGAAGAATTAAAGTTTTTCCCAAATATTAATCAATCAAAAGATTCTGTTGATTATAAGAACGTA
ATTGAATTAAATAATCAATCTAATTTTGAATCAAGCAAAGATACTTTAGTTAAAGAGGACAAATCAGAAAAAGCTATTGT
TCCATTACCTAAAAGTGAACTTCAAATCAAAACCAAAAAACAAGTTGAACAAGATGAAAATGTTCTTTTAATTTATGGAA
AAGATTTAACAGAATCAGCTAAAAAAGGCTTATTGGATCCTGTTATAGGAAGAGAAAATGAGATCAATAATTTAATGAGG
GTGCTCTGCCGAAGAAACAAAAATAACCCCATACTTATCGGCAATCCAGGAGTTGGTAAAACCTCAATTGCGAAATTACT
TGCACAATTAATTGTAGACAAAAAAGTTCCTGATTCTTTAAAAGACTTTAAAATTATTTCACTTGACTTGGGGGCATTAG
TTTCTGGGACAAAATTCAGAGGCCAACTAGAAGAACGATTAAGCTTAATCCTGCAGGAATTAAAAGATCCAAGCCAAGGA
ATGATTTTATTTATTGATGAAATTCACTCAATATTAAGTTCTGACAGATCTACTACAGATATTAGTAATATCTTAAAACC
TTTACTAGCTGAAGGAGAACTTAGATGTATTGGTACAACCACACCTGAGAAATTTCGTGAAACTATTGAAAAAGATCAGG
CATTGAATAATTGCTTTCAAAAGATAGCTGTTAATGAACCTTCAGTAGAATTGAGCGCAAAAATACTGCAAGGTATCAAA
AAGAAATATGAATTACATCATGGCATCAGAATTTCTGAAGACGCAGTTAATTATTCTGCAAAACTGGCCGACAGATACAT
CAACGATAAATGTCTTCCTGATAAAGCAATAGATTTAATTGATGAAGCAGCTGCACAGTTAAAAATCGAGTCTAATAAAA
AGCCTCAAATAATTCTCCAACAAGAAAACAAAATTTATACTATCGATGAAAAACTGAATAATTTGCAAGGTGAAAATATC
GAAGTGCAAGAAAAACTATTAAATATGAAGCAACAAGCACAGTCAAAATTGAATGTTCTTTTTGAGAATTGGAATAATTT
GCGGGAAAAAATGGAACAATTAGCTATCTTGATGACAGAAAAAGATAAGCTAACTAAACAAATTAAAGACAAATCAAATC
ACAAGATTGAAAATAATCTGAATTTTTTAGAAAAACTTGAAGAAGATTTAAGTGAAATTGAGAATGAAATACAAAACGTT
GAAGAAAATTTTAATAAAATAAAGAAAAATAGAAATTCCCCTTTTAAATATCAAGTTGAACCTGATGATATCGCTGATGT
TATCTCAAAAATCACAGGTATTCCAATTTCTAAAGTCGTTTCAAATGAACGTAAGAAATTAGTCAATCTTGAGAAAGAAT
TAAGTGAAAAAGTTATTGGACAAGAAAAAGCTATAGAAGCTGTTTCTGCTGCAATCAGAAGGGCTCGCGTTGGCATGAAA
AGTCCTAAAAGACCTATTGGATCTTTTTTATTTATGGGTCCTACAGGTGTTGGTAAAACAGAATTAGCAAAGTCTCTTGC
ATCATCTTTATTTGATGAAGAAGAGGCACTTTTAAGATTAGACATGAGTGAATATATGGAGAAAAATGCAGTAGCAAGAC
TTTTGGGAGCTCCACCAGGTTATGTTGGTTATGAAGAGGGAGGACAATTAACTGAAGCTGTCAGACGCAAACCCTATTCA
GTAATACTTCTTGATGAGATAGAAAAAGCTCATACAGAAGTTTTTAATATCCTTTTACAAGTCTTAGATGAAGGAAGATT
AACTGATTCTCAAGGAAGGACCGTAGACTTCAAAAATACAGTAATCATTATGACAAGTAACCTAGCCGGGAAATTTATAC
TTGAGTATTCACAAAAGATTTCTAAGAGTGAGGGAAAGTTAGAAAAAGATCAACAAATTCTAGATGATTCTATTAGTAAT
ACATTGTCTTCAATTTTTAGACCGGAATTTTTAAATAGAATTGATGAAGTAGTAAAGTTTGATCCATTATCTATTGATGA
ACTTCAAAAAATAATTATTCTACAAACAGAAGATTTAAAAAATCTGCTTCTTGAACAGAAAATAAATATCACTATAGACA
AAAAAGTTATCAATAAAATTGCAAACGATTCTTACGAACCTAAATATGGCGCTAGACCTCTTAGTAGGGAACTTAGAAGA
CAAATAGAAAATCCCTTAGCTGCAAAACTTTTAGAGGATAACTTTAAAAACAAAAAAAATATCACAATTAAACTTAACCC
CTCTAAAAAGGATGAGATTGTTTTCAGACCTAGCTGA

Upstream 100 bases:

>100_bases
CTTTTAAATTTCATCTTACAATTTGAAAATTATTGGATGATTATTAATGCAGATTCTATTTAGAATATTAATTAATAATC
AACTATAGTTTTTATAGTTT

Downstream 100 bases:

>100_bases
GGAGTAAATTGATAAGCTAAAATAAAAATTAAAGCGTAAAGCTTAATTTCAAAAAAAATTTGTGACAAGTCCTACTACTA
ATAAAGAGCCTCTAAAAAAG

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 918; Mature: 918

Protein sequence:

>918_residues
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSREFFAFIEKLSIDQDTVLDITEDFLEETPTNES
DIFTIGEDLEILLDNANQIKIQWGSRLIEIPHLLIALGRDLRIGNYVFEEGNLSMEKLEEELKFFPNINQSKDSVDYKNV
IELNNQSNFESSKDTLVKEDKSEKAIVPLPKSELQIKTKKQVEQDENVLLIYGKDLTESAKKGLLDPVIGRENEINNLMR
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDSLKDFKIISLDLGALVSGTKFRGQLEERLSLILQELKDPSQG
MILFIDEIHSILSSDRSTTDISNILKPLLAEGELRCIGTTTPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIK
KKYELHHGIRISEDAVNYSAKLADRYINDKCLPDKAIDLIDEAAAQLKIESNKKPQIILQQENKIYTIDEKLNNLQGENI
EVQEKLLNMKQQAQSKLNVLFENWNNLREKMEQLAILMTEKDKLTKQIKDKSNHKIENNLNFLEKLEEDLSEIENEIQNV
EENFNKIKKNRNSPFKYQVEPDDIADVISKITGIPISKVVSNERKKLVNLEKELSEKVIGQEKAIEAVSAAIRRARVGMK
SPKRPIGSFLFMGPTGVGKTELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
VILLDEIEKAHTEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKFILEYSQKISKSEGKLEKDQQILDDSISN
TLSSIFRPEFLNRIDEVVKFDPLSIDELQKIIILQTEDLKNLLLEQKINITIDKKVINKIANDSYEPKYGARPLSRELRR
QIENPLAAKLLEDNFKNKKNITIKLNPSKKDEIVFRPS

Sequences:

>Translated_918_residues
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSREFFAFIEKLSIDQDTVLDITEDFLEETPTNES
DIFTIGEDLEILLDNANQIKIQWGSRLIEIPHLLIALGRDLRIGNYVFEEGNLSMEKLEEELKFFPNINQSKDSVDYKNV
IELNNQSNFESSKDTLVKEDKSEKAIVPLPKSELQIKTKKQVEQDENVLLIYGKDLTESAKKGLLDPVIGRENEINNLMR
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDSLKDFKIISLDLGALVSGTKFRGQLEERLSLILQELKDPSQG
MILFIDEIHSILSSDRSTTDISNILKPLLAEGELRCIGTTTPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIK
KKYELHHGIRISEDAVNYSAKLADRYINDKCLPDKAIDLIDEAAAQLKIESNKKPQIILQQENKIYTIDEKLNNLQGENI
EVQEKLLNMKQQAQSKLNVLFENWNNLREKMEQLAILMTEKDKLTKQIKDKSNHKIENNLNFLEKLEEDLSEIENEIQNV
EENFNKIKKNRNSPFKYQVEPDDIADVISKITGIPISKVVSNERKKLVNLEKELSEKVIGQEKAIEAVSAAIRRARVGMK
SPKRPIGSFLFMGPTGVGKTELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
VILLDEIEKAHTEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKFILEYSQKISKSEGKLEKDQQILDDSISN
TLSSIFRPEFLNRIDEVVKFDPLSIDELQKIIILQTEDLKNLLLEQKINITIDKKVINKIANDSYEPKYGARPLSRELRR
QIENPLAAKLLEDNFKNKKNITIKLNPSKKDEIVFRPS
>Mature_918_residues
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSREFFAFIEKLSIDQDTVLDITEDFLEETPTNES
DIFTIGEDLEILLDNANQIKIQWGSRLIEIPHLLIALGRDLRIGNYVFEEGNLSMEKLEEELKFFPNINQSKDSVDYKNV
IELNNQSNFESSKDTLVKEDKSEKAIVPLPKSELQIKTKKQVEQDENVLLIYGKDLTESAKKGLLDPVIGRENEINNLMR
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDSLKDFKIISLDLGALVSGTKFRGQLEERLSLILQELKDPSQG
MILFIDEIHSILSSDRSTTDISNILKPLLAEGELRCIGTTTPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIK
KKYELHHGIRISEDAVNYSAKLADRYINDKCLPDKAIDLIDEAAAQLKIESNKKPQIILQQENKIYTIDEKLNNLQGENI
EVQEKLLNMKQQAQSKLNVLFENWNNLREKMEQLAILMTEKDKLTKQIKDKSNHKIENNLNFLEKLEEDLSEIENEIQNV
EENFNKIKKNRNSPFKYQVEPDDIADVISKITGIPISKVVSNERKKLVNLEKELSEKVIGQEKAIEAVSAAIRRARVGMK
SPKRPIGSFLFMGPTGVGKTELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
VILLDEIEKAHTEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKFILEYSQKISKSEGKLEKDQQILDDSISN
TLSSIFRPEFLNRIDEVVKFDPLSIDELQKIIILQTEDLKNLLLEQKINITIDKKVINKIANDSYEPKYGARPLSRELRR
QIENPLAAKLLEDNFKNKKNITIKLNPSKKDEIVFRPS

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=323, Percent_Identity=39.3188854489164, Blast_Score=213, Evalue=6e-55,
Organism=Escherichia coli, GI1788943, Length=709, Percent_Identity=45.6981664315938, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1787109, Length=340, Percent_Identity=42.3529411764706, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6320464, Length=739, Percent_Identity=41.4073071718539, Blast_Score=542, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6323002, Length=927, Percent_Identity=34.0884573894283, Blast_Score=494, Evalue=1e-140,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 104608; Mature: 104608

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSREFFAFIEKLSID
CCCCCCCCCHHHHCCCCCEEEEECHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
QDTVLDITEDFLEETPTNESDIFTIGEDLEILLDNANQIKIQWGSRLIEIPHLLIALGRD
CHHHHHHHHHHHHHCCCCCCCEEEECCCHHEEECCCCEEEEECCCHHHHHHHHHHHHCCC
LRIGNYVFEEGNLSMEKLEEELKFFPNINQSKDSVDYKNVIELNNQSNFESSKDTLVKED
CEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCHHHHHHCC
KSEKAIVPLPKSELQIKTKKQVEQDENVLLIYGKDLTESAKKGLLDPVIGRENEINNLMR
CCCCCEECCCHHHHEECHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDSLKDFKIISLDLGALVSGTKFR
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEHHHHHCCCHHH
GQLEERLSLILQELKDPSQGMILFIDEIHSILSSDRSTTDISNILKPLLAEGELRCIGTT
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECC
TPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIKKKYELHHGIRISEDAVNYSA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHH
KLADRYINDKCLPDKAIDLIDEAAAQLKIESNKKPQIILQQENKIYTIDEKLNNLQGENI
HHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCCEEEHHHHHCCCCCCCC
EVQEKLLNMKQQAQSKLNVLFENWNNLREKMEQLAILMTEKDKLTKQIKDKSNHKIENNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHH
NFLEKLEEDLSEIENEIQNVEENFNKIKKNRNSPFKYQVEPDDIADVISKITGIPISKVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCHHHHH
SNERKKLVNLEKELSEKVIGQEKAIEAVSAAIRRARVGMKSPKRPIGSFLFMGPTGVGKT
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHH
ELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCE
VILLDEIEKAHTEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKFILEYSQKI
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHH
SKSEGKLEKDQQILDDSISNTLSSIFRPEFLNRIDEVVKFDPLSIDELQKIIILQTEDLK
HHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHEEECHHHH
NLLLEQKINITIDKKVINKIANDSYEPKYGARPLSRELRRQIENPLAAKLLEDNFKNKKN
HHHHHHHCCEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCE
ITIKLNPSKKDEIVFRPS
EEEEECCCCCCCEEECCC
>Mature Secondary Structure
MRETLTSSPELFSDISWNLLLLGEETAKKWDHSEFNIEHIIHTLFSSREFFAFIEKLSID
CCCCCCCCCHHHHCCCCCEEEEECHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCC
QDTVLDITEDFLEETPTNESDIFTIGEDLEILLDNANQIKIQWGSRLIEIPHLLIALGRD
CHHHHHHHHHHHHHCCCCCCCEEEECCCHHEEECCCCEEEEECCCHHHHHHHHHHHHCCC
LRIGNYVFEEGNLSMEKLEEELKFFPNINQSKDSVDYKNVIELNNQSNFESSKDTLVKED
CEECCEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHEECCCCCCCCCHHHHHHCC
KSEKAIVPLPKSELQIKTKKQVEQDENVLLIYGKDLTESAKKGLLDPVIGRENEINNLMR
CCCCCEECCCHHHHEECHHHHHCCCCCEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHH
VLCRRNKNNPILIGNPGVGKTSIAKLLAQLIVDKKVPDSLKDFKIISLDLGALVSGTKFR
HHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCHHCCEEEEEEHHHHHCCCHHH
GQLEERLSLILQELKDPSQGMILFIDEIHSILSSDRSTTDISNILKPLLAEGELRCIGTT
HHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEECC
TPEKFRETIEKDQALNNCFQKIAVNEPSVELSAKILQGIKKKYELHHGIRISEDAVNYSA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEECHHHHHHHH
KLADRYINDKCLPDKAIDLIDEAAAQLKIESNKKPQIILQQENKIYTIDEKLNNLQGENI
HHHHHHCCCCCCCHHHHHHHHHHHHHEEECCCCCCEEEEECCCCEEEHHHHHCCCCCCCC
EVQEKLLNMKQQAQSKLNVLFENWNNLREKMEQLAILMTEKDKLTKQIKDKSNHKIENNL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHH
NFLEKLEEDLSEIENEIQNVEENFNKIKKNRNSPFKYQVEPDDIADVISKITGIPISKVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCHHHHH
SNERKKLVNLEKELSEKVIGQEKAIEAVSAAIRRARVGMKSPKRPIGSFLFMGPTGVGKT
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHH
ELAKSLASSLFDEEEALLRLDMSEYMEKNAVARLLGAPPGYVGYEEGGQLTEAVRRKPYS
HHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHCCCCE
VILLDEIEKAHTEVFNILLQVLDEGRLTDSQGRTVDFKNTVIIMTSNLAGKFILEYSQKI
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCEEEEEECCCCHHHHHHHHHHH
SKSEGKLEKDQQILDDSISNTLSSIFRPEFLNRIDEVVKFDPLSIDELQKIIILQTEDLK
HHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHEEECHHHH
NLLLEQKINITIDKKVINKIANDSYEPKYGARPLSRELRRQIENPLAAKLLEDNFKNKKN
HHHHHHHCCEEECHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHCCCCCE
ITIKLNPSKKDEIVFRPS
EEEEECCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]