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The map label for this gene is rnhA [H]

Identifier: 78778588

GI number: 78778588

Start: 194846

End: 195565

Strand: Reverse

Name: rnhA [H]

Synonym: PMT9312_0202

Alternate gene names: 78778588

Gene position: 195565-194846 (Counterclockwise)

Preceding gene: 78778589

Following gene: 78778587

Centisome position: 11.44

GC content: 31.25

Gene sequence:

>720_bases
ATGAATAGCGATTGTATTGCAATTGAAGCTGCAACTGATGGAGCCTGCAGCGGTAATCCAGGTCCAGGGGGTTGGGGTGG
TTTAATAATTTTTGACGATAACAGTGAATTAGAAATAGGTGGTTCCGAGCAAAATACCACTAATAATAGAATGGAATTAA
CTGCAGCTATAAAAACTCTTGAAAAATTAACAACCTATAAATTAAAAGAGAACTTTAAATTAAGAACCGATAGTAAATAT
GTAATAGAAGGGTATACAAAATGGATTATAAACTGGAAGAGAAATGGATGGAAAACAAGCGCAGGAAAACCAGTACAGAA
TCTTGATTTATGGCAAAAAATTGATCAATTAAGAATTAATGGCCTAATAATGGAATATGTTAAGGGTCATAGCGGGGATA
AACAAAATGATAGGGTTGATAAAATAGCAACTAATTACAGCAAAGGTATATCTATAGGAAGTAACTTAAAAGAAGCAGAA
TCCTCAGTTGACCTTTTTGAAAAAAATGCACCTGCAGAAATTCAGGCATTATTTTCAAGAAATGAATTAATTCAAAAATT
TGCAGAAAAAAGTTACTTGTTAAGTTCATTAGAACTGAGTAACTTATTGGGCAAAGAAAACCACTTAAAGATAAAACAAT
ATTTACTTTTTGAATGGCGTAATTGGAGATTGATTCCTAAAGATAAAAAATATTGGATAATAGAAAAAAAAGAATCCTAA

Upstream 100 bases:

>100_bases
AATCTCTAAAAAAAGAGATTGAAGAAGCTGGCGGTAAGGTTACACTTAAGTAAGAGTACATTTTTTTAAGCCGATAACCT
TTATATCGGCTTCAAAAATT

Downstream 100 bases:

>100_bases
TTTTTTATGAATGAACAGAAGGGGGTATTTAATAAACTTTATAAAAGCTTGATGACCCCTATACTAAAAAAAGACTCTGG
AATAGATGCAGAATATTTAA

Product: RNase HI

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNSDCIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTLEKLTTYKLKENFKLRTDSKY
VIEGYTKWIINWKRNGWKTSAGKPVQNLDLWQKIDQLRINGLIMEYVKGHSGDKQNDRVDKIATNYSKGISIGSNLKEAE
SSVDLFEKNAPAEIQALFSRNELIQKFAEKSYLLSSLELSNLLGKENHLKIKQYLLFEWRNWRLIPKDKKYWIIEKKES

Sequences:

>Translated_239_residues
MNSDCIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTLEKLTTYKLKENFKLRTDSKY
VIEGYTKWIINWKRNGWKTSAGKPVQNLDLWQKIDQLRINGLIMEYVKGHSGDKQNDRVDKIATNYSKGISIGSNLKEAE
SSVDLFEKNAPAEIQALFSRNELIQKFAEKSYLLSSLELSNLLGKENHLKIKQYLLFEWRNWRLIPKDKKYWIIEKKES
>Mature_239_residues
MNSDCIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTLEKLTTYKLKENFKLRTDSKY
VIEGYTKWIINWKRNGWKTSAGKPVQNLDLWQKIDQLRINGLIMEYVKGHSGDKQNDRVDKIATNYSKGISIGSNLKEAE
SSVDLFEKNAPAEIQALFSRNELIQKFAEKSYLLSSLELSNLLGKENHLKIKQYLLFEWRNWRLIPKDKKYWIIEKKES

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=141, Percent_Identity=35.4609929078014, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786408, Length=139, Percent_Identity=48.2014388489209, Blast_Score=125, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI115532542, Length=136, Percent_Identity=39.7058823529412, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17505482, Length=129, Percent_Identity=37.2093023255814, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI17137476, Length=137, Percent_Identity=31.3868613138686, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45552509, Length=137, Percent_Identity=31.3868613138686, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 27254; Mature: 27254

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDCIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTL
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHH
EKLTTYKLKENFKLRTDSKYVIEGYTKWIINWKRNGWKTSAGKPVQNLDLWQKIDQLRIN
HHHHHHHHHCCCEEECCCEEEEECEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GLIMEYVKGHSGDKQNDRVDKIATNYSKGISIGSNLKEAESSVDLFEKNAPAEIQALFSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
NELIQKFAEKSYLLSSLELSNLLGKENHLKIKQYLLFEWRNWRLIPKDKKYWIIEKKES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCEECCCCCEEEEEEECCC
>Mature Secondary Structure
MNSDCIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTL
CCCCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHH
EKLTTYKLKENFKLRTDSKYVIEGYTKWIINWKRNGWKTSAGKPVQNLDLWQKIDQLRIN
HHHHHHHHHCCCEEECCCEEEEECEEEEEEEECCCCEECCCCCCCHHHHHHHHHHHHHHH
GLIMEYVKGHSGDKQNDRVDKIATNYSKGISIGSNLKEAESSVDLFEKNAPAEIQALFSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
NELIQKFAEKSYLLSSLELSNLLGKENHLKIKQYLLFEWRNWRLIPKDKKYWIIEKKES
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHEEECCCCEECCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]