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The map label for this gene is prfB

Identifier: 78778568

GI number: 78778568

Start: 174115

End: 175179

Strand: Direct

Name: prfB

Synonym: PMT9312_0182

Alternate gene names: 78778568

Gene position: 174115-175179 (Clockwise)

Preceding gene: 78778565

Following gene: 78778569

Centisome position: 10.19

GC content: 35.21

Gene sequence:

>1065_bases
ATGCTCAGGATTGTCTTTGACGTACCAAGATTAAAAGCAAAAAGGAGAGAGTTAGAGCAAATTTCTGCTCAGCCTGAATT
TTGGGAGAATCAAGAAGCAGCTAAAAAGCAAATGTTAATCCTTGATGATGTCAAAGCCCAACTCGAATTGTTAGATAAAT
GGAAAACTTTTATTTCTGATGCTAATGCCTCTCTTGAGCTTTATTCTTTAGAACCTGAAGAGGAAATGATTTTGGAGTCC
CAGCAGGGCTTAAAGAAATTAAGAGATAATTTAGATAAATGGGAATTCGAAAGATTGTTATGTGGTGAATACGATAAGGA
AGGAGCAGTAGTTTCTATTAAAGCAGGTGCCGGTGGAACAGATGCGCAGGATTGGGTAGAGATATTATTGAGAATGTATT
CAAGATGGGCCGATAATAATCAAATGAGCCTTATAATCAATGAATTATCTCAAGGAGAAGAAGCAGGTATTAAAAGTGTA
ACGTTTGAAATTGATGGAAAATATGCATACGGCTATCTGCAATATGAAAAAGGAACTCATAGATTAGTAAGAATTTCTCC
TTTTAATGCTAATGGCAAAAGGCAAACCAGCTTTGCTGGGGTAGAGGTTATGCCAAAATTAGATGATAATATTTCGCTTG
ATATACCTGAAAAAGATTTAGAAATTACAACGAGTAGATCCGGTGGAGCGGGAGGACAAAATGTTAATAAAGTAGAAACA
GCAGTAAGAATTGTTCATTTGCCTTCCGGGATTTCAGTAAGATGCACCCAAGAAAGATCTCAATTACAAAATAAAGAAAA
AGCTATGTTACTTCTAAAGTCTAAACTATTAGTGATTGCTAAAGAACAACGAGCTGCTGAAATCGCTGATATTAAAGGTG
ATATTGTAGAAGCTGCATGGGGCAATCAAATAAGAAATTATGTTTTCCATCCCTATCAAATGGTAAAAGATCTTAGGACT
ATGCAGGAGACTAACGAGTTAGATAGTGTTTTAGATGGTGGACTTGAACCATTTATTCATGAATTATTACGTATGAATAT
TTCTTCGAACGAATCTATTGAATAA

Upstream 100 bases:

>100_bases
CAAAAGTGCTTGTTAGATTATAAATAACGATTAAATTCTATTTTGATAGATATTACAGATTTCAAAAAAGATCTTTCGGA
ACTGACAGTGCGCCTGGGTA

Downstream 100 bases:

>100_bases
CTAATGGAAAATAAAAAAGAAATTTTAGAAAAAAAAGTTGCTCCTCCAAGCTTTATAAAGCTTGCTATGAGAAATATGGT
AAGGAAAGGTTCAAAAAGTA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLRIVFDVPRLKAKRRELEQISAQPEFWENQEAAKKQMLILDDVKAQLELLDKWKTFISDANASLELYSLEPEEEMILES
QQGLKKLRDNLDKWEFERLLCGEYDKEGAVVSIKAGAGGTDAQDWVEILLRMYSRWADNNQMSLIINELSQGEEAGIKSV
TFEIDGKYAYGYLQYEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEIADIKGDIVEAAWGNQIRNYVFHPYQMVKDLRT
MQETNELDSVLDGGLEPFIHELLRMNISSNESIE

Sequences:

>Translated_354_residues
MLRIVFDVPRLKAKRRELEQISAQPEFWENQEAAKKQMLILDDVKAQLELLDKWKTFISDANASLELYSLEPEEEMILES
QQGLKKLRDNLDKWEFERLLCGEYDKEGAVVSIKAGAGGTDAQDWVEILLRMYSRWADNNQMSLIINELSQGEEAGIKSV
TFEIDGKYAYGYLQYEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEIADIKGDIVEAAWGNQIRNYVFHPYQMVKDLRT
MQETNELDSVLDGGLEPFIHELLRMNISSNESIE
>Mature_354_residues
MLRIVFDVPRLKAKRRELEQISAQPEFWENQEAAKKQMLILDDVKAQLELLDKWKTFISDANASLELYSLEPEEEMILES
QQGLKKLRDNLDKWEFERLLCGEYDKEGAVVSIKAGAGGTDAQDWVEILLRMYSRWADNNQMSLIINELSQGEEAGIKSV
TFEIDGKYAYGYLQYEKGTHRLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEIADIKGDIVEAAWGNQIRNYVFHPYQMVKDLRT
MQETNELDSVLDGGLEPFIHELLRMNISSNESIE

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=237, Percent_Identity=35.0210970464135, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI34577120, Length=281, Percent_Identity=32.3843416370107, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI166795305, Length=147, Percent_Identity=34.6938775510204, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=42.3076923076923, Blast_Score=267, Evalue=8e-73,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=38.6831275720165, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17542784, Length=231, Percent_Identity=29.8701298701299, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321295, Length=234, Percent_Identity=30.3418803418803, Blast_Score=103, Evalue=5e-23,
Organism=Drosophila melanogaster, GI19921226, Length=210, Percent_Identity=36.6666666666667, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40238; Mature: 40238

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRIVFDVPRLKAKRRELEQISAQPEFWENQEAAKKQMLILDDVKAQLELLDKWKTFISD
CEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ANASLELYSLEPEEEMILESQQGLKKLRDNLDKWEFERLLCGEYDKEGAVVSIKAGAGGT
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCC
DAQDWVEILLRMYSRWADNNQMSLIINELSQGEEAGIKSVTFEIDGKYAYGYLQYEKGTH
CHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCEEEEEEECCEEEEEEEEEECCCC
RLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
EEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCHHHHHH
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEIADIKGDIVEAAW
EEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHH
GNQIRNYVFHPYQMVKDLRTMQETNELDSVLDGGLEPFIHELLRMNISSNESIE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLRIVFDVPRLKAKRRELEQISAQPEFWENQEAAKKQMLILDDVKAQLELLDKWKTFISD
CEEEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ANASLELYSLEPEEEMILESQQGLKKLRDNLDKWEFERLLCGEYDKEGAVVSIKAGAGGT
CCCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCEEEEECCCCCC
DAQDWVEILLRMYSRWADNNQMSLIINELSQGEEAGIKSVTFEIDGKYAYGYLQYEKGTH
CHHHHHHHHHHHHHHHCCCCCCEEEEHHHCCCCCCCCEEEEEEECCEEEEEEEEEECCCC
RLVRISPFNANGKRQTSFAGVEVMPKLDDNISLDIPEKDLEITTSRSGGAGGQNVNKVET
EEEEEECCCCCCCCCCCCCCEEEEECCCCCEEEECCCCCCEEEECCCCCCCCCCHHHHHH
AVRIVHLPSGISVRCTQERSQLQNKEKAMLLLKSKLLVIAKEQRAAEIADIKGDIVEAAW
EEEEEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHEEEECHHHHHHHHHCCCHHHHHHH
GNQIRNYVFHPYQMVKDLRTMQETNELDSVLDGGLEPFIHELLRMNISSNESIE
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917641 [H]