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The map label for this gene is pchA [H]

Identifier: 78778565

GI number: 78778565

Start: 171392

End: 172804

Strand: Direct

Name: pchA [H]

Synonym: PMT9312_0179

Alternate gene names: 78778565

Gene position: 171392-172804 (Clockwise)

Preceding gene: 78778561

Following gene: 78778568

Centisome position: 10.03

GC content: 25.76

Gene sequence:

>1413_bases
ATGAAAAATGATTTAAAGTTTACAGATTTTTTAAAAGATTTATTTTCCACTTTCGATAAGAAGGTGGAAAATTCTGGATT
AGTAAGTATTTGTGTTGAGATACCTTGTATTGATTTATTTCAAGTATATGAATTGTTTATAAATAAATATCAGTTTTCTT
CATTTTGGGAGGAATCTGATGGTATTTCCTACATTGCTTTCGATAAATGTAAATATGTTACTTTGGATGGTCCAAGAAGA
TATGAGGTAGCAAAAGAGTTTAATACTGAAAATTTTAAAAATTTAATTAACTTAACAAATGAATCACATATTTGCTCACT
TTCAAAAATAATTTATTTATTTTCTTTTTCTGAAAATTTGAATAATAAGAATTTATCTTCAGATGTTCCTAGTTTGGAAG
CTATCTTACCAAAAATATTAATTATCAAAAGCGATAATAATTGCTGGTTAAGAATTAATGGTCATGTTGAAGGTAAGTCA
TCATTAAGAATATTAATTGAAGAAATATGGGCAATTAGAAATCAAGTTATTAATTCTGGCTCAGAATTAAAAAAATTATC
TAGTTTAAAAAATAGTTTTGATTTTCCTATTATTGATGATTTCTTAAGTTCCCTTAAAATTTCTAATGTAAATTTGAAAA
AAATAGTAAATAAAGGAATTCAATTAGTAGATAAGGGTATCCTCGAAAAGATAGTTTTAGCAAATAGGATTAAAATAAAA
CTTAAAAATAAATTAGATTTAGTAGAAATTTTAAAAAGATTAAAAAATAATCAACCAAATACATGCAGATACGTTTGGAA
AAGAAATAGTAAAGATATTTTTTTTGGAGCATCTCCAGAAAAATTATTTTCTTTTACTAAACCAAATTTAACTTTTGAGG
CTCTTGCCGGAACGATCTCAACTAACTCTAATTTTAATAACCTCCTTGGAAGTTCTAAAGACTTAAAGGAACATAATTAT
GTAATAAACTATTTAATTAAATGTTTAGAAATTTTAAAAATAAAAAAATTTAAAAAAAGTGATATCAAGGTAAATTCATT
TGGAGATATTTCACATTTGCAAACACTTATTTTCTCTAAAGTTGAAAATATATGTCCTTTTGAATTGCTTAAAAATTTGC
ATCCATCGCCGGCTGTTTGTGGATATCCTAAGAATGAGGCATTGTATTGGATTAATACTCTTGAGTCTTTTGCTAGAGGA
AATTATGCTTCTCCAATGGGCTGGGTTGATTCATCAGGAAATGCTTCATTTCTTGTAGCAATAAGAGGCGCTAGATATAT
TGAAGAAAATATTGAATTCACTGCCGGTTCAGGGATAGTTTCAGGATCTGTTTTAGAAAAAGAAATAGATGAGATTAGAT
TAAAATTTGAATCTTTAGTAAAACAAATAGTTTTTGCTAAAACTTCTAAATAA

Upstream 100 bases:

>100_bases
TTTAATTAACACACAAAATTCCATCATTATTAAATATAGTTTATAAATTGAGTTACATTTTTTACTTTATTGCATGATTT
TTAGTTATTTTCAATAAGTA

Downstream 100 bases:

>100_bases
TTTCTATTAACTTTTCAATAACTTCTTCTGAAACATTTTTATTAGATAAATTTTCTATTTCTCTTAAACCTGTTGGACTG
GTTACATTAATCTCGCTAAG

Product: isochorismate synthase

Products: NA

Alternate protein names: Isochorismate mutase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MKNDLKFTDFLKDLFSTFDKKVENSGLVSICVEIPCIDLFQVYELFINKYQFSSFWEESDGISYIAFDKCKYVTLDGPRR
YEVAKEFNTENFKNLINLTNESHICSLSKIIYLFSFSENLNNKNLSSDVPSLEAILPKILIIKSDNNCWLRINGHVEGKS
SLRILIEEIWAIRNQVINSGSELKKLSSLKNSFDFPIIDDFLSSLKISNVNLKKIVNKGIQLVDKGILEKIVLANRIKIK
LKNKLDLVEILKRLKNNQPNTCRYVWKRNSKDIFFGASPEKLFSFTKPNLTFEALAGTISTNSNFNNLLGSSKDLKEHNY
VINYLIKCLEILKIKKFKKSDIKVNSFGDISHLQTLIFSKVENICPFELLKNLHPSPAVCGYPKNEALYWINTLESFARG
NYASPMGWVDSSGNASFLVAIRGARYIEENIEFTAGSGIVSGSVLEKEIDEIRLKFESLVKQIVFAKTSK

Sequences:

>Translated_470_residues
MKNDLKFTDFLKDLFSTFDKKVENSGLVSICVEIPCIDLFQVYELFINKYQFSSFWEESDGISYIAFDKCKYVTLDGPRR
YEVAKEFNTENFKNLINLTNESHICSLSKIIYLFSFSENLNNKNLSSDVPSLEAILPKILIIKSDNNCWLRINGHVEGKS
SLRILIEEIWAIRNQVINSGSELKKLSSLKNSFDFPIIDDFLSSLKISNVNLKKIVNKGIQLVDKGILEKIVLANRIKIK
LKNKLDLVEILKRLKNNQPNTCRYVWKRNSKDIFFGASPEKLFSFTKPNLTFEALAGTISTNSNFNNLLGSSKDLKEHNY
VINYLIKCLEILKIKKFKKSDIKVNSFGDISHLQTLIFSKVENICPFELLKNLHPSPAVCGYPKNEALYWINTLESFARG
NYASPMGWVDSSGNASFLVAIRGARYIEENIEFTAGSGIVSGSVLEKEIDEIRLKFESLVKQIVFAKTSK
>Mature_470_residues
MKNDLKFTDFLKDLFSTFDKKVENSGLVSICVEIPCIDLFQVYELFINKYQFSSFWEESDGISYIAFDKCKYVTLDGPRR
YEVAKEFNTENFKNLINLTNESHICSLSKIIYLFSFSENLNNKNLSSDVPSLEAILPKILIIKSDNNCWLRINGHVEGKS
SLRILIEEIWAIRNQVINSGSELKKLSSLKNSFDFPIIDDFLSSLKISNVNLKKIVNKGIQLVDKGILEKIVLANRIKIK
LKNKLDLVEILKRLKNNQPNTCRYVWKRNSKDIFFGASPEKLFSFTKPNLTFEALAGTISTNSNFNNLLGSSKDLKEHNY
VINYLIKCLEILKIKKFKKSDIKVNSFGDISHLQTLIFSKVENICPFELLKNLHPSPAVCGYPKNEALYWINTLESFARG
NYASPMGWVDSSGNASFLVAIRGARYIEENIEFTAGSGIVSGSVLEKEIDEIRLKFESLVKQIVFAKTSK

Specific function: Involved in the conversion of chorismate to salicylate (Probable) [H]

COG id: COG1169

COG function: function code HQ; Isochorismate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786809, Length=262, Percent_Identity=27.4809160305344, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082077, Length=306, Percent_Identity=23.202614379085, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR015890
- InterPro:   IPR004561 [H]

Pfam domain/function: PF00425 Chorismate_bind [H]

EC number: =5.4.4.2 [H]

Molecular weight: Translated: 53616; Mature: 53616

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNDLKFTDFLKDLFSTFDKKVENSGLVSICVEIPCIDLFQVYELFINKYQFSSFWEESD
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
GISYIAFDKCKYVTLDGPRRYEVAKEFNTENFKNLINLTNESHICSLSKIIYLFSFSENL
CCEEEEECCEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCC
NNKNLSSDVPSLEAILPKILIIKSDNNCWLRINGHVEGKSSLRILIEEIWAIRNQVINSG
CCCCCCCCCCHHHHHCCEEEEEECCCCEEEEEECEECCCHHHHHHHHHHHHHHHHHHCCC
SELKKLSSLKNSFDFPIIDDFLSSLKISNVNLKKIVNKGIQLVDKGILEKIVLANRIKIK
HHHHHHHHHHHCCCCCHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LKNKLDLVEILKRLKNNQPNTCRYVWKRNSKDIFFGASPEKLFSFTKPNLTFEALAGTIS
ECCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHCCCCCCCHHHHHCCCC
TNSNFNNLLGSSKDLKEHNYVINYLIKCLEILKIKKFKKSDIKVNSFGDISHLQTLIFSK
CCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHH
VENICPFELLKNLHPSPAVCGYPKNEALYWINTLESFARGNYASPMGWVDSSGNASFLVA
HHCCCHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
IRGARYIEENIEFTAGSGIVSGSVLEKEIDEIRLKFESLVKQIVFAKTSK
ECCCHHHHHCCCEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKNDLKFTDFLKDLFSTFDKKVENSGLVSICVEIPCIDLFQVYELFINKYQFSSFWEESD
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
GISYIAFDKCKYVTLDGPRRYEVAKEFNTENFKNLINLTNESHICSLSKIIYLFSFSENL
CCEEEEECCEEEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHEECCCCC
NNKNLSSDVPSLEAILPKILIIKSDNNCWLRINGHVEGKSSLRILIEEIWAIRNQVINSG
CCCCCCCCCCHHHHHCCEEEEEECCCCEEEEEECEECCCHHHHHHHHHHHHHHHHHHCCC
SELKKLSSLKNSFDFPIIDDFLSSLKISNVNLKKIVNKGIQLVDKGILEKIVLANRIKIK
HHHHHHHHHHHCCCCCHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LKNKLDLVEILKRLKNNQPNTCRYVWKRNSKDIFFGASPEKLFSFTKPNLTFEALAGTIS
ECCCCCHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHCCCCCCCHHHHHCCCC
TNSNFNNLLGSSKDLKEHNYVINYLIKCLEILKIKKFKKSDIKVNSFGDISHLQTLIFSK
CCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHH
VENICPFELLKNLHPSPAVCGYPKNEALYWINTLESFARGNYASPMGWVDSSGNASFLVA
HHCCCHHHHHHCCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
IRGARYIEENIEFTAGSGIVSGSVLEKEIDEIRLKFESLVKQIVFAKTSK
ECCCHHHHHCCCEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7500944; 10984043 [H]