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The map label for this gene is lacA [C]

Identifier: 78358289

GI number: 78358289

Start: 3247667

End: 3248332

Strand: Reverse

Name: lacA [C]

Synonym: Dde_3249

Alternate gene names: 78358289

Gene position: 3248332-3247667 (Counterclockwise)

Preceding gene: 78358290

Following gene: 78358288

Centisome position: 87.08

GC content: 50.15

Gene sequence:

>666_bases
ATGTTGCGCTCTCAATTTGAAATTCTCTATCCCGGTGTTAGTTTCGGGAATAATGTGCAGATATTGGGAATGGCTAACGT
TGATATCGGCACAGGCTCCTGCATCGGTGATAATGCATGGCTTAATGTCTGTCAGCGGGACAATGAGAAGCGTATAAAGA
TTGGTCGTTGTGTGCTTGTCGGGCGCGGTTCGATGATTTCTGCAGGCGGCTTTCTTGAGATCGGTGATTACTGCCTTTTT
GCTCCCCGGGTTTTTGTAGCGGATGCAGACCACGTTTATAGCAACATCATGCGGCCTTATATAGATCAGGGCGCAACGTC
CGGTAGAGTGGTGGTGGAGGAAAACTGCTGGCTCGGAGTCAATGTAGCGGTTTCGGGCAATGTTGTGATCGGGCGGGGGA
GTGTTGTAGGAGCAAGTGCCGTTGTTACCAGAGACGTTCCTCCTTTTTCCGTAGTGGTTGGGGCACCTGCCCGGATTATA
CAGCTTTTTGATTTTGAGGTCGGAGGATGGGTGAAGATTCGTGACGACTGTCACCTGAGTGGGCTTCTGGAGGCAAGAGA
AAGGCATCCTGTTCCTTCACGCAGCGTGTATGCACAGATGCTTCACAAAACTGCCGTAACAAAAACAGTGAACCCTCTTG
TGGCAGGTGGCGGTGTTTCAATATAA

Upstream 100 bases:

>100_bases
GACTCTGCCAAGATACGATTGTATGGAATTTCCTTTTGGGAAAAGCTACCAATCTCTCCCGCATCCACGGCGGGCATCAG
GCCTTTAAAAACCGGAGGGA

Downstream 100 bases:

>100_bases
TTCAGAAACTGTCCGTCACTTCTACGCGTGATTTCGTCTTCATGCCTCCGCCTGCGTGCAACAAGAAAAGCCCTCTGCAT
ACATGTATGCAGAGGGCTTT

Product: acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 221

Protein sequence:

>221_residues
MLRSQFEILYPGVSFGNNVQILGMANVDIGTGSCIGDNAWLNVCQRDNEKRIKIGRCVLVGRGSMISAGGFLEIGDYCLF
APRVFVADADHVYSNIMRPYIDQGATSGRVVVEENCWLGVNVAVSGNVVIGRGSVVGASAVVTRDVPPFSVVVGAPARII
QLFDFEVGGWVKIRDDCHLSGLLEARERHPVPSRSVYAQMLHKTAVTKTVNPLVAGGGVSI

Sequences:

>Translated_221_residues
MLRSQFEILYPGVSFGNNVQILGMANVDIGTGSCIGDNAWLNVCQRDNEKRIKIGRCVLVGRGSMISAGGFLEIGDYCLF
APRVFVADADHVYSNIMRPYIDQGATSGRVVVEENCWLGVNVAVSGNVVIGRGSVVGASAVVTRDVPPFSVVVGAPARII
QLFDFEVGGWVKIRDDCHLSGLLEARERHPVPSRSVYAQMLHKTAVTKTVNPLVAGGGVSI
>Mature_221_residues
MLRSQFEILYPGVSFGNNVQILGMANVDIGTGSCIGDNAWLNVCQRDNEKRIKIGRCVLVGRGSMISAGGFLEIGDYCLF
APRVFVADADHVYSNIMRPYIDQGATSGRVVVEENCWLGVNVAVSGNVVIGRGSVVGASAVVTRDVPPFSVVVGAPARII
QLFDFEVGGWVKIRDDCHLSGLLEARERHPVPSRSVYAQMLHKTAVTKTVNPLVAGGGVSI

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=130, Percent_Identity=30.7692307692308, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1788345, Length=108, Percent_Identity=36.1111111111111, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 23661; Mature: 23661

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRSQFEILYPGVSFGNNVQILGMANVDIGTGSCIGDNAWLNVCQRDNEKRIKIGRCVLV
CCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEECCHHHHHHHHCCCCCEEEEEEEEEE
GRGSMISAGGFLEIGDYCLFAPRVFVADADHVYSNIMRPYIDQGATSGRVVVEENCWLGV
ECCCEEECCCCEEECCEEEECCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEE
NVAVSGNVVIGRGSVVGASAVVTRDVPPFSVVVGAPARIIQLFDFEVGGWVKIRDDCHLS
EEEEECCEEEECCCEECCEEEEECCCCCEEEEECCHHHEEEEEEECCCCEEEECCCCCHH
GLLEARERHPVPSRSVYAQMLHKTAVTKTVNPLVAGGGVSI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC
>Mature Secondary Structure
MLRSQFEILYPGVSFGNNVQILGMANVDIGTGSCIGDNAWLNVCQRDNEKRIKIGRCVLV
CCCCCEEEEECCCCCCCCEEEEEEEEEECCCCCEECCHHHHHHHHCCCCCEEEEEEEEEE
GRGSMISAGGFLEIGDYCLFAPRVFVADADHVYSNIMRPYIDQGATSGRVVVEENCWLGV
ECCCEEECCCCEEECCEEEECCEEEEECCHHHHHHHHHHHHHCCCCCCEEEEECCCEEEE
NVAVSGNVVIGRGSVVGASAVVTRDVPPFSVVVGAPARIIQLFDFEVGGWVKIRDDCHLS
EEEEECCEEEECCCEECCEEEEECCCCCEEEEECCHHHEEEEEEECCCCEEEECCCCCHH
GLLEARERHPVPSRSVYAQMLHKTAVTKTVNPLVAGGGVSI
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]