Click here to switch to the map view.

The map label for this gene is fixL [H]

Identifier: 78355896

GI number: 78355896

Start: 879698

End: 881803

Strand: Direct

Name: fixL [H]

Synonym: Dde_0849

Alternate gene names: 78355896

Gene position: 879698-881803 (Clockwise)

Preceding gene: 78355895

Following gene: 78355897

Centisome position: 23.58

GC content: 57.64

Gene sequence:

>2106_bases
ATGACGCTGATATTGTCAGTATTTCTGGGAGCGCTGCTGGCTGCGGCTTTCTGCGTTTACACGGTGCGCACTGCCGGTAT
GCGCAGGGCGCTGACTGTCATTCCCGCGGTGGGTGTGACAGCAGCCGTGGCAGCGGCCGAAGGTGCCCTGCAGTATCTCA
GTGTGCCTGCCCATGGTCTGCTGCAACCGGTGCTGCTGCTGCAGGGGTTGTTATGTCCGGCGTGGCTGCTGTTCGGACTG
GCGTATGCACGCGGTTTTTCGTGGCAGGGGCTCAGCCTGCTCAACCGCCTGTTGCTTTTCGCTTCTTTTATTCCCGCTTT
GCTGGTGGTGCTGTTTCCGGTTTCCCGTTTTCTGTTTTTGCCTGATTTTACGCTGGAGCAGGTTATCTATCTGGAGCCTT
TGGCTTTTTCGTTGTATCTGCTCATCATTTTGTTTCTTCTTGTGGCGTTGGGCAATCTGGAAGCCACACTGCGCAACGCG
CGGCACAGTGACCGCTGGCGTGTGAAGCTGGCTTTGGTGGGCGGCGGTACCGTGCTTATGGCCCATGTCCTTTTTTACAG
TCAGGGCCTGCTGCACCGTTCCATAGACATGAACTACGGCGGGCTGCGGACGGCAGGCAGCATGCTGGGCATAGCCATGC
TGTTTTTTGCCGAGCTGCGGCGCGAAAGCGGCAGGGTGACGGTTACCGGCAGGTTTGCTTTGCGTTCCACCGTGGTGCTG
CTGGCGGGCGTATACCTTATAGGGCTGGGCATTGCGCGTGAAGGAGTGCGGCATTTCGGCTCGGAGTTCGAGCGGCACGC
GGCTATCGGTCTTTTGTTTCTGCTGGGACTGGCGGTGGTGCTGGTGCTGCTTTCCGAACGGGTGCGCAGACGGCTGCGCA
TATGGCTGCAGCGTAATTTTTATCAGGACAAGTATGACTACCGCAGCCAGTGGATGGAATTTACAAGGCGTCTGGCCGGA
GTGCGCGGTATGGACGAACTTACCCGCACGGTACTGCTGCATTATTGCGAGGCCTTCGGTTTTGTGGGCGGGGCGTGGTT
TGCGGCGGACCATGAAACACAGGGCAGAGTGACGGCTGGTGAGTACTACGAAATGGACGAGCCGCAAGTTATGCCGGAGT
CCATTGATTTTTCACTGATTATGGAACTGCAGGCTCCTGTGGCCATACCGGTGCTCAAAGACCGGCTGGCTGCGGCAGGA
CGTATGGAGGAAGCAGGGTGTCTCGATTTTGTGCGTGCCACCAGAGCCGAGGTTGTGGTGCCTGTTGCGGCAGGAGACAG
CTTTGAAGGGCTGCTGCTTCTCGGGCCGGCCATAGACCGTTCGGAAGCCTATGACACCGAAGATTTTGAGCTGATGGATG
CCATGGCTCACGAGGTGGGGCTTACGGTGAAAAGTTTTCGTCTGGGCAACGAGCTTGCGGCGGCGCGAGAGATGGAAGCC
ATGGGCAAAGTGGCTGCTTTTGTGCTGCACGACCTGAAAAATCAGGTTTATACGTTGTCTCTGGTAACGGAAAATGCCCG
CGACTTCATCGGCGAACCTGATTTTCAGGCCGATATGCTGGAAAGTCTGGAAAACACCGTGACCAAGATGAAGGTGCTGA
TAACGCAGCTGAAACACCTGCCCGATGCCGGAAGCCTGCGTCTTGCGCCTGTGGATATGCACGAACTGGTGCGTAAGGCG
GCTTTGCAGGTGCCCGGCGTCCGGCTGGTTGTGCAGGGGCCGCAGCTTGTTGCTGCCGTTGATGAAGAGCAGATTGAAAA
AGTGTTTGTGAATCTGTTTCTGAACGCGGTGGAAGCGGGCGGTGACCGGCCGGTCACTGTAACTACGGTGCAGCAACCTG
TATTTTCAGTTTCTGTGCGTGACAGCGCCGGGGGCATAGACAGAGACATTCTGCGCCGGGGGCTGTTCAAACCGTTCCGC
AGTACCAAAAGACGCGGGATGGGCATTGGTCTTTACCATTGCAAAAAAATTGTCGAGGCGCACGGCGCCGCGCTGCATGT
GCGGAACAGGCCGGGAGAAGGGGCAGAATTTGTCATTGAATTCGCCCCGCAGCAGGAGAAGCCGCAGAGCGCGGGCAACG
GCGGTGCAAAGGGCGCTTCTGTCTGA

Upstream 100 bases:

>100_bases
AGCCTGATGAGGTGGCAACGCGTTTCATCAGTGATTTTTATCCTGTTCTGAACACCTTTCTGCCGCGCTGACAGGTGCCG
CACACCGGGGACGGTATGGT

Downstream 100 bases:

>100_bases
TGCCGCATGGTTTTCTGCATATCCGCAGCCACAGAGCACGGTGCGGCAGAAAACCGGACCACAGGGGATTACCATGCCGA
AATTGCTGATTGTTGATGAC

Product: periplasmic sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 701; Mature: 700

Protein sequence:

>701_residues
MTLILSVFLGALLAAAFCVYTVRTAGMRRALTVIPAVGVTAAVAAAEGALQYLSVPAHGLLQPVLLLQGLLCPAWLLFGL
AYARGFSWQGLSLLNRLLLFASFIPALLVVLFPVSRFLFLPDFTLEQVIYLEPLAFSLYLLIILFLLVALGNLEATLRNA
RHSDRWRVKLALVGGGTVLMAHVLFYSQGLLHRSIDMNYGGLRTAGSMLGIAMLFFAELRRESGRVTVTGRFALRSTVVL
LAGVYLIGLGIAREGVRHFGSEFERHAAIGLLFLLGLAVVLVLLSERVRRRLRIWLQRNFYQDKYDYRSQWMEFTRRLAG
VRGMDELTRTVLLHYCEAFGFVGGAWFAADHETQGRVTAGEYYEMDEPQVMPESIDFSLIMELQAPVAIPVLKDRLAAAG
RMEEAGCLDFVRATRAEVVVPVAAGDSFEGLLLLGPAIDRSEAYDTEDFELMDAMAHEVGLTVKSFRLGNELAAAREMEA
MGKVAAFVLHDLKNQVYTLSLVTENARDFIGEPDFQADMLESLENTVTKMKVLITQLKHLPDAGSLRLAPVDMHELVRKA
ALQVPGVRLVVQGPQLVAAVDEEQIEKVFVNLFLNAVEAGGDRPVTVTTVQQPVFSVSVRDSAGGIDRDILRRGLFKPFR
STKRRGMGIGLYHCKKIVEAHGAALHVRNRPGEGAEFVIEFAPQQEKPQSAGNGGAKGASV

Sequences:

>Translated_701_residues
MTLILSVFLGALLAAAFCVYTVRTAGMRRALTVIPAVGVTAAVAAAEGALQYLSVPAHGLLQPVLLLQGLLCPAWLLFGL
AYARGFSWQGLSLLNRLLLFASFIPALLVVLFPVSRFLFLPDFTLEQVIYLEPLAFSLYLLIILFLLVALGNLEATLRNA
RHSDRWRVKLALVGGGTVLMAHVLFYSQGLLHRSIDMNYGGLRTAGSMLGIAMLFFAELRRESGRVTVTGRFALRSTVVL
LAGVYLIGLGIAREGVRHFGSEFERHAAIGLLFLLGLAVVLVLLSERVRRRLRIWLQRNFYQDKYDYRSQWMEFTRRLAG
VRGMDELTRTVLLHYCEAFGFVGGAWFAADHETQGRVTAGEYYEMDEPQVMPESIDFSLIMELQAPVAIPVLKDRLAAAG
RMEEAGCLDFVRATRAEVVVPVAAGDSFEGLLLLGPAIDRSEAYDTEDFELMDAMAHEVGLTVKSFRLGNELAAAREMEA
MGKVAAFVLHDLKNQVYTLSLVTENARDFIGEPDFQADMLESLENTVTKMKVLITQLKHLPDAGSLRLAPVDMHELVRKA
ALQVPGVRLVVQGPQLVAAVDEEQIEKVFVNLFLNAVEAGGDRPVTVTTVQQPVFSVSVRDSAGGIDRDILRRGLFKPFR
STKRRGMGIGLYHCKKIVEAHGAALHVRNRPGEGAEFVIEFAPQQEKPQSAGNGGAKGASV
>Mature_700_residues
TLILSVFLGALLAAAFCVYTVRTAGMRRALTVIPAVGVTAAVAAAEGALQYLSVPAHGLLQPVLLLQGLLCPAWLLFGLA
YARGFSWQGLSLLNRLLLFASFIPALLVVLFPVSRFLFLPDFTLEQVIYLEPLAFSLYLLIILFLLVALGNLEATLRNAR
HSDRWRVKLALVGGGTVLMAHVLFYSQGLLHRSIDMNYGGLRTAGSMLGIAMLFFAELRRESGRVTVTGRFALRSTVVLL
AGVYLIGLGIAREGVRHFGSEFERHAAIGLLFLLGLAVVLVLLSERVRRRLRIWLQRNFYQDKYDYRSQWMEFTRRLAGV
RGMDELTRTVLLHYCEAFGFVGGAWFAADHETQGRVTAGEYYEMDEPQVMPESIDFSLIMELQAPVAIPVLKDRLAAAGR
MEEAGCLDFVRATRAEVVVPVAAGDSFEGLLLLGPAIDRSEAYDTEDFELMDAMAHEVGLTVKSFRLGNELAAAREMEAM
GKVAAFVLHDLKNQVYTLSLVTENARDFIGEPDFQADMLESLENTVTKMKVLITQLKHLPDAGSLRLAPVDMHELVRKAA
LQVPGVRLVVQGPQLVAAVDEEQIEKVFVNLFLNAVEAGGDRPVTVTTVQQPVFSVSVRDSAGGIDRDILRRGLFKPFRS
TKRRGMGIGLYHCKKIVEAHGAALHVRNRPGEGAEFVIEFAPQQEKPQSAGNGGAKGASV

Specific function: Putative oxygen sensor; modulates the activity of fixJ, a transcriptional activator of nitrogen fixation fixK gene. FixL probably acts as a kinase that phosphorylates fixJ [H]

COG id: COG4191

COG function: function code T; Signal transduction histidine kinase regulating C4-dicarboxylate transport system

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=24.7826086956522, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=25.2212389380531, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 77043; Mature: 76912

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLILSVFLGALLAAAFCVYTVRTAGMRRALTVIPAVGVTAAVAAAEGALQYLSVPAHGL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LQPVLLLQGLLCPAWLLFGLAYARGFSWQGLSLLNRLLLFASFIPALLVVLFPVSRFLFL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDFTLEQVIYLEPLAFSLYLLIILFLLVALGNLEATLRNARHSDRWRVKLALVGGGTVLM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCHHHHH
AHVLFYSQGLLHRSIDMNYGGLRTAGSMLGIAMLFFAELRRESGRVTVTGRFALRSTVVL
HHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHH
LAGVYLIGLGIAREGVRHFGSEFERHAAIGLLFLLGLAVVLVLLSERVRRRLRIWLQRNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQDKYDYRSQWMEFTRRLAGVRGMDELTRTVLLHYCEAFGFVGGAWFAADHETQGRVTAG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEECC
EYYEMDEPQVMPESIDFSLIMELQAPVAIPVLKDRLAAAGRMEEAGCLDFVRATRAEVVV
CCCCCCCCCCCCCCCCHHHEEECCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEE
PVAAGDSFEGLLLLGPAIDRSEAYDTEDFELMDAMAHEVGLTVKSFRLGNELAAAREMEA
EEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHH
MGKVAAFVLHDLKNQVYTLSLVTENARDFIGEPDFQADMLESLENTVTKMKVLITQLKHL
HHHHHHHHHHHHHCCEEEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PDAGSLRLAPVDMHELVRKAALQVPGVRLVVQGPQLVAAVDEEQIEKVFVNLFLNAVEAG
CCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHCC
GDRPVTVTTVQQPVFSVSVRDSAGGIDRDILRRGLFKPFRSTKRRGMGIGLYHCKKIVEA
CCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HGAALHVRNRPGEGAEFVIEFAPQQEKPQSAGNGGAKGASV
CCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLILSVFLGALLAAAFCVYTVRTAGMRRALTVIPAVGVTAAVAAAEGALQYLSVPAHGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LQPVLLLQGLLCPAWLLFGLAYARGFSWQGLSLLNRLLLFASFIPALLVVLFPVSRFLFL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDFTLEQVIYLEPLAFSLYLLIILFLLVALGNLEATLRNARHSDRWRVKLALVGGGTVLM
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEEEEEEECCHHHHH
AHVLFYSQGLLHRSIDMNYGGLRTAGSMLGIAMLFFAELRRESGRVTVTGRFALRSTVVL
HHHHHHHCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHH
LAGVYLIGLGIAREGVRHFGSEFERHAAIGLLFLLGLAVVLVLLSERVRRRLRIWLQRNF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YQDKYDYRSQWMEFTRRLAGVRGMDELTRTVLLHYCEAFGFVGGAWFAADHETQGRVTAG
CCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEECC
EYYEMDEPQVMPESIDFSLIMELQAPVAIPVLKDRLAAAGRMEEAGCLDFVRATRAEVVV
CCCCCCCCCCCCCCCCHHHEEECCCCEEHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEE
PVAAGDSFEGLLLLGPAIDRSEAYDTEDFELMDAMAHEVGLTVKSFRLGNELAAAREMEA
EEECCCCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHH
MGKVAAFVLHDLKNQVYTLSLVTENARDFIGEPDFQADMLESLENTVTKMKVLITQLKHL
HHHHHHHHHHHHHCCEEEEEEEECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
PDAGSLRLAPVDMHELVRKAALQVPGVRLVVQGPQLVAAVDEEQIEKVFVNLFLNAVEAG
CCCCCEEECCCCHHHHHHHHHHCCCCEEEEEECCCEEECCCHHHHHHHHHHHHHHHHHCC
GDRPVTVTTVQQPVFSVSVRDSAGGIDRDILRRGLFKPFRSTKRRGMGIGLYHCKKIVEA
CCCCEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
HGAALHVRNRPGEGAEFVIEFAPQQEKPQSAGNGGAKGASV
CCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 8432704 [H]