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The map label for this gene is wcaJ [H]

Identifier: 78355876

GI number: 78355876

Start: 854550

End: 855893

Strand: Direct

Name: wcaJ [H]

Synonym: Dde_0829

Alternate gene names: 78355876

Gene position: 854550-855893 (Clockwise)

Preceding gene: 78355875

Following gene: 78355877

Centisome position: 22.91

GC content: 55.88

Gene sequence:

>1344_bases
ATGACCGTCAGTACATTTTCCACGCTGGTTCAGGATACCCTGTGGCTGCTTCTGGCCCTGCTGCTGGCTGCCATGCCTGC
ACTGGGCCACGGAGGGCTTGCCGGACTGCCGCCGTTATCGGACGCAGCCCTGCTGGCACTGGTGATTCTGGCATGCACGC
TGATTGTCGAAAGGCTGGATTCCGGCCACGGCAACAACAGCCGCGCGGTTATCAGAATCACTGCGGCGGCGCTTTTGCTG
GCGTCACTGGTAATTCTGGGACTGCGCATGGGCAGTGGTCTGTTTGAACGGCAGGAAGGGCTGGCGGTTGCAGTAATGGG
CTGGTTTGCCCTGTTCCGTTGCGGAGGTACAATGATTCGCCGTTACTGGGAGTTTATTCCTGTACTTGCTCCCGGCGTGC
TGATTGTGGGCAACGGCGAGCTGGCCGACGCCATGCGTACGCTGGTGCTGGAAAGCAGGGGGCGTTATCGCCTGAAAGGT
TTTGTGGAATGTTCGGCCAACGGCGGGGAACTTGCCGATACGCTGGACGGCCTGAGCCTGCTTGAATATGCGCGCAAAAA
TAAAGTGCGTAAAATGGTTGTGTCACTGACGGAACGCAGGGGAGCTTTTCCTCTCGACGAAGTGCTGCGCTGCCGTATGA
ATGGTATCGAGGTGGTGGACGCAGCCAGTTTTTATGAAGAAGCCAATAAAAAGCTCTACATAGAGAATATTACCCCCAGC
TGGTTCATTTTTTCGCAGGGGTTCAGGATTTCAGCGTGGCGCAGGCTGGGCAAGCGCGTGATGGATGTGGTGTTTTCTTC
GCTGGGGCTGTTGCTTACCGCTCCTTTTTTTCCGCTGATGGCGCTGCTTATCAAGCTTGACTCACCCGGGCCTGTGTTTT
TCCGGCAGGTTCGTGTGGGGGCCGGAGACAGTGAGTTTGTCATTTTCAAATTCCGCTCCATGCGCTGCGACGCGGAAGAA
AAGACAGGAGCCGTATGGGCCAAGGTCAACGACCCGCGGGTGACAAGGCTGGGCAACTTTCTGCGGCGCAGCCGCATAGA
TGAAATTCCACAGCTTATCAATGTGATCCGCGGTGAGATGAGTCTGGTGGGACCTCGTCCGGAAAGACCGGAATTTGTCA
GAACACTGCGCCGGCAGATACCGTTTTATTCCGAGCGGCATTACATGAAGCCCGGCGTGACCGGCTGGGCGCAGGTGCGC
TATCCCTATGGCGCCAGCGAGGAAGACGCCATAGAAAAGTTGCGCTATGACCTGTACTACATAAAAAACTATTCTATTCT
CTTTGATTTACGTATCATGCTCGATACCGTGGGTGTTGTTCTTTTCCGCAAGGGAGGCCGCTAG

Upstream 100 bases:

>100_bases
AGAAGTCAGCACCGCTCTGCAGGGCAGCGAGTTTTCCGAGGCAGGGGAAGCCCGGAAGCTGCTCAAAAAACTGGGCGGGT
AAGACCATACGGAGGGGGAT

Downstream 100 bases:

>100_bases
TGAAAAAGGTACTGCTGTTGTTGGTTGTTGTGCTGCTTTTTGGCGCCGTAGCGGCTCAGGCCGCGGACGGTGAATACGTC
ATCGGCAGCGGCGACGGGCT

Product: lipopolysaccharide synthesis sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTVSTFSTLVQDTLWLLLALLLAAMPALGHGGLAGLPPLSDAALLALVILACTLIVERLDSGHGNNSRAVIRITAAALLL
ASLVILGLRMGSGLFERQEGLAVAVMGWFALFRCGGTMIRRYWEFIPVLAPGVLIVGNGELADAMRTLVLESRGRYRLKG
FVECSANGGELADTLDGLSLLEYARKNKVRKMVVSLTERRGAFPLDEVLRCRMNGIEVVDAASFYEEANKKLYIENITPS
WFIFSQGFRISAWRRLGKRVMDVVFSSLGLLLTAPFFPLMALLIKLDSPGPVFFRQVRVGAGDSEFVIFKFRSMRCDAEE
KTGAVWAKVNDPRVTRLGNFLRRSRIDEIPQLINVIRGEMSLVGPRPERPEFVRTLRRQIPFYSERHYMKPGVTGWAQVR
YPYGASEEDAIEKLRYDLYYIKNYSILFDLRIMLDTVGVVLFRKGGR

Sequences:

>Translated_447_residues
MTVSTFSTLVQDTLWLLLALLLAAMPALGHGGLAGLPPLSDAALLALVILACTLIVERLDSGHGNNSRAVIRITAAALLL
ASLVILGLRMGSGLFERQEGLAVAVMGWFALFRCGGTMIRRYWEFIPVLAPGVLIVGNGELADAMRTLVLESRGRYRLKG
FVECSANGGELADTLDGLSLLEYARKNKVRKMVVSLTERRGAFPLDEVLRCRMNGIEVVDAASFYEEANKKLYIENITPS
WFIFSQGFRISAWRRLGKRVMDVVFSSLGLLLTAPFFPLMALLIKLDSPGPVFFRQVRVGAGDSEFVIFKFRSMRCDAEE
KTGAVWAKVNDPRVTRLGNFLRRSRIDEIPQLINVIRGEMSLVGPRPERPEFVRTLRRQIPFYSERHYMKPGVTGWAQVR
YPYGASEEDAIEKLRYDLYYIKNYSILFDLRIMLDTVGVVLFRKGGR
>Mature_446_residues
TVSTFSTLVQDTLWLLLALLLAAMPALGHGGLAGLPPLSDAALLALVILACTLIVERLDSGHGNNSRAVIRITAAALLLA
SLVILGLRMGSGLFERQEGLAVAVMGWFALFRCGGTMIRRYWEFIPVLAPGVLIVGNGELADAMRTLVLESRGRYRLKGF
VECSANGGELADTLDGLSLLEYARKNKVRKMVVSLTERRGAFPLDEVLRCRMNGIEVVDAASFYEEANKKLYIENITPSW
FIFSQGFRISAWRRLGKRVMDVVFSSLGLLLTAPFFPLMALLIKLDSPGPVFFRQVRVGAGDSEFVIFKFRSMRCDAEEK
TGAVWAKVNDPRVTRLGNFLRRSRIDEIPQLINVIRGEMSLVGPRPERPEFVRTLRRQIPFYSERHYMKPGVTGWAQVRY
PYGASEEDAIEKLRYDLYYIKNYSILFDLRIMLDTVGVVLFRKGGR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=192, Percent_Identity=45.8333333333333, Blast_Score=165, Evalue=5e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 50069; Mature: 49937

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSTFSTLVQDTLWLLLALLLAAMPALGHGGLAGLPPLSDAALLALVILACTLIVERLD
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SGHGNNSRAVIRITAAALLLASLVILGLRMGSGLFERQEGLAVAVMGWFALFRCGGTMIR
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCHHHHH
RYWEFIPVLAPGVLIVGNGELADAMRTLVLESRGRYRLKGFVECSANGGELADTLDGLSL
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHH
LEYARKNKVRKMVVSLTERRGAFPLDEVLRCRMNGIEVVDAASFYEEANKKLYIENITPS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHCCCEEEEEECCCC
WFIFSQGFRISAWRRLGKRVMDVVFSSLGLLLTAPFFPLMALLIKLDSPGPVFFRQVRVG
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEEEEC
AGDSEFVIFKFRSMRCDAEEKTGAVWAKVNDPRVTRLGNFLRRSRIDEIPQLINVIRGEM
CCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
SLVGPRPERPEFVRTLRRQIPFYSERHYMKPGVTGWAQVRYPYGASEEDAIEKLRYDLYY
HCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHE
IKNYSILFDLRIMLDTVGVVLFRKGGR
EECCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TVSTFSTLVQDTLWLLLALLLAAMPALGHGGLAGLPPLSDAALLALVILACTLIVERLD
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
SGHGNNSRAVIRITAAALLLASLVILGLRMGSGLFERQEGLAVAVMGWFALFRCGGTMIR
CCCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHCHHHHH
RYWEFIPVLAPGVLIVGNGELADAMRTLVLESRGRYRLKGFVECSANGGELADTLDGLSL
HHHHHHHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHHH
LEYARKNKVRKMVVSLTERRGAFPLDEVLRCRMNGIEVVDAASFYEEANKKLYIENITPS
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHHHHHHHCCCEEEEEECCCC
WFIFSQGFRISAWRRLGKRVMDVVFSSLGLLLTAPFFPLMALLIKLDSPGPVFFRQVRVG
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHEEEEC
AGDSEFVIFKFRSMRCDAEEKTGAVWAKVNDPRVTRLGNFLRRSRIDEIPQLINVIRGEM
CCCCCEEEEEEECCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCHH
SLVGPRPERPEFVRTLRRQIPFYSERHYMKPGVTGWAQVRYPYGASEEDAIEKLRYDLYY
HCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHE
IKNYSILFDLRIMLDTVGVVLFRKGGR
EECCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]