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The map label for this gene is kinE [H]

Identifier: 78355805

GI number: 78355805

Start: 783280

End: 785682

Strand: Direct

Name: kinE [H]

Synonym: Dde_0758

Alternate gene names: 78355805

Gene position: 783280-785682 (Clockwise)

Preceding gene: 78355802

Following gene: 78355806

Centisome position: 21.0

GC content: 58.18

Gene sequence:

>2403_bases
ATGACGAAGTCAATTTTATGTCCAGGAGCAAGGATGGGAATTTTTCAACACCCTGTTACCGATGATGCGCTGTATCAGGC
GGTGCAGCTGCCTTCTTTTTCTTATTATATGGTGGCCAGCACACGCATGCAGTCTGTTATGGAGCTGGTGCAGCCGCTTG
TGGTGCGTACCGTTATTCTGGCAGCCGGTCTGTGGGTGGTGGGCATTTTTCTGACAATCGTGGTCTACAGGCATTCGGTC
AGTCAGTACGATCTGGCTCTGTCCGAACTGCAGTGTACGCAGCGTGACGAACTGGCAAATCTGTTCAAGGCGCTTGTCAC
GGACGTGCAGGCTGCCCCTGAACCGGCAACGGCCATGGAAGTTTTTCTGCGGCGTCTTTGCGAGCGGACGGGGTTTGAAC
TGGGAGCCGTGTACCTGCCGGACAAGCAGGAACGTTACCGCTTTGTGATTTATCAGAGTGATTCTTTTCACCCCATTGCC
AGAAATCCGGGCAAAGGCATGTCCGCACCGGACGTGCTGCGTGCGCATCTGCTGGGGCTTATGCAGCACCCTGTGTTTTT
GCAGGCAGAACAGAACGTGGTTGTGCCGGACATGAGCGTTGATGAGCGGTTTGCGGAATTTTATCCGGCCGGGGAGGGCT
CGCCCGCGCAGCCGCTGCTGAAAACTGTAATCAGGGGAGCCTGCGTTTCCCGTGTGCGCCGTGCTTCCGTGGGGCATGTG
CATGTCGGTCTGTTCTCTCTTGTATCGCTGGAAAAAAGCAGTGATGTCGCCGGACTGCTTGATGAAGTTATGCCGCTGCT
GGGGCCGGTGGCTGAGAGTAAGCTTGCAGAGGTGCACCTGCGCGAACAGGAAATGCTCTTCCGCAACCTGTATGACAGGT
CGGCTCTGGTGCAGGTGGTGGTCAATCCTGAAACAGGCACGGTGCTGGATGCCAACATGGCGGCGTGCGAATTTTACGGC
TATCCGCACGAAGTGTTTGTGACACAGACCCTGTATGACCTGTGGCAGGCTCCACGGGACGATATTGACGCCGTCATGTC
CGCTTCCACAGAACTGTACACCGGTTTGCACATACAGAAGCATATTCTGGCCGGCGGCGACCTGAAGGATGTGGAGGCGT
ACATGGGCAGGGTACCGTGGAAGGGGCAGCATGCCCTGCTTTGCGCCGTGTTCGACCGTTCCGGCATAAAGAAGATGGAG
GCGGCTCTGCTGCGCAGCGAGCAGCAGCTTGCCATGCTGATGGAAAAGCTGCCTCTTGCGCTGGCGCTGCTGGATGAGAC
CGGAACCATACGGCAGGTGAACGGGGCTTTTGTAGCGCTTACAGGCCGCACGGCGATTTCCTGTACCGGCAGGTATCTGC
ATGAGCTGGGGCTGGAAAGTCTGCATGAGGCGTCATGTCCTGTTGTGCAGGATGCCCTGCTGCCGGCCGGCAGAGAGCCG
CAGGAGCTGCTTCTTGTCACGTTGCAGGGTGAAGAACGCCATGTGCTTGTACATACCACCCGTCTGCAAATGCACGAGGA
GGGCACACGGTATGCGGCGTTCATAGTGGATATGACCGAACGGCGCAGGCTGGACGACCTTGTGGTGCAAAGTGAAAAAA
TGGCCTCCGTCGGGGGGCTTGCCGCGGGCATGGCGCACGAGATCAATAACCCGCTGGGCGGCATAATGCAGGGAGCGCAG
AACATCATCAGGCGGCTTGATCCGCACCGGGAGGCGAACCGTGCCGCGGCACAGGCGTGCGGGTGTCCGCTCGAAGCCAT
CAGGGCATACCTTGAGCAGCGCGGCATCCTGCGGTTTATCGGCGGCATAGAGGATTCTGCCCGCCGCGCTGCCCGTATAA
CGTCAAATATGCTTGAATTCAGCCGGCCTTGTGCCGGAGAATGGCGCGAGGCACGCCTTGAGCATATTCTGGATGTGGCG
CTGGAGCTGGCAGCTAATGACTATGATCTGAAAAAACGTTATGACTTCAGAACAATCCGGATCGTCAGAGACTATTCGCC
CGATGTTCCTGCCGTGCGCTGCATCGTTTCCGAAGTGGAACAGGTGGCGCTCAACTTGCTGCGTAATGCCGCACAGGCCA
TGGCGGAAGCCGCCACAGAGGCTCCTGCCATCACGCTGCGCACACGGGTGGACGGCGAATACGCTGTTTTTGAGGTGGAG
GACAACGGGCCCGGCATGAGCAGAGAAGTGGCCCGCCGCGTTTTTGAGCCGTTTTTCACCACCAGAGAACCGGGGCGCGG
CACAGGTCTCGGGTTGTCTGTGTCGTATTTTATCATTACCGCCACTCACAAGGGGGCGATAGCAGTGCAGGCCCGGCCCG
ACCGCGGAACAGCATTTGTGGTGCGCCTGCCCTTGCGCAGTGCAGAGTGTCTGCACGAAGAAAAACCTGCCGTGGAGAAC
TGA

Upstream 100 bases:

>100_bases
AACCAAAACAATAGCAAATAAGGTCGGACATTTTTATCTCCTGGGTCAGCTCAATTTAATCAATAGCATGTTTCCTTCCA
AAGAAACTACAGGACGGTTC

Downstream 100 bases:

>100_bases
CATGCTTTTTACCCTGTGCCGTCCGGTGTGCCGCCCGGCGTGCCGTGTGCATCTGCCCGCCCGCGCTGTTGCAGTGCTGC
TTTTTATCCTGTTTGCCGTG

Product: PAS/PAC sensor signal transduction histidine kinase

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 800; Mature: 799

Protein sequence:

>800_residues
MTKSILCPGARMGIFQHPVTDDALYQAVQLPSFSYYMVASTRMQSVMELVQPLVVRTVILAAGLWVVGIFLTIVVYRHSV
SQYDLALSELQCTQRDELANLFKALVTDVQAAPEPATAMEVFLRRLCERTGFELGAVYLPDKQERYRFVIYQSDSFHPIA
RNPGKGMSAPDVLRAHLLGLMQHPVFLQAEQNVVVPDMSVDERFAEFYPAGEGSPAQPLLKTVIRGACVSRVRRASVGHV
HVGLFSLVSLEKSSDVAGLLDEVMPLLGPVAESKLAEVHLREQEMLFRNLYDRSALVQVVVNPETGTVLDANMAACEFYG
YPHEVFVTQTLYDLWQAPRDDIDAVMSASTELYTGLHIQKHILAGGDLKDVEAYMGRVPWKGQHALLCAVFDRSGIKKME
AALLRSEQQLAMLMEKLPLALALLDETGTIRQVNGAFVALTGRTAISCTGRYLHELGLESLHEASCPVVQDALLPAGREP
QELLLVTLQGEERHVLVHTTRLQMHEEGTRYAAFIVDMTERRRLDDLVVQSEKMASVGGLAAGMAHEINNPLGGIMQGAQ
NIIRRLDPHREANRAAAQACGCPLEAIRAYLEQRGILRFIGGIEDSARRAARITSNMLEFSRPCAGEWREARLEHILDVA
LELAANDYDLKKRYDFRTIRIVRDYSPDVPAVRCIVSEVEQVALNLLRNAAQAMAEAATEAPAITLRTRVDGEYAVFEVE
DNGPGMSREVARRVFEPFFTTREPGRGTGLGLSVSYFIITATHKGAIAVQARPDRGTAFVVRLPLRSAECLHEEKPAVEN

Sequences:

>Translated_800_residues
MTKSILCPGARMGIFQHPVTDDALYQAVQLPSFSYYMVASTRMQSVMELVQPLVVRTVILAAGLWVVGIFLTIVVYRHSV
SQYDLALSELQCTQRDELANLFKALVTDVQAAPEPATAMEVFLRRLCERTGFELGAVYLPDKQERYRFVIYQSDSFHPIA
RNPGKGMSAPDVLRAHLLGLMQHPVFLQAEQNVVVPDMSVDERFAEFYPAGEGSPAQPLLKTVIRGACVSRVRRASVGHV
HVGLFSLVSLEKSSDVAGLLDEVMPLLGPVAESKLAEVHLREQEMLFRNLYDRSALVQVVVNPETGTVLDANMAACEFYG
YPHEVFVTQTLYDLWQAPRDDIDAVMSASTELYTGLHIQKHILAGGDLKDVEAYMGRVPWKGQHALLCAVFDRSGIKKME
AALLRSEQQLAMLMEKLPLALALLDETGTIRQVNGAFVALTGRTAISCTGRYLHELGLESLHEASCPVVQDALLPAGREP
QELLLVTLQGEERHVLVHTTRLQMHEEGTRYAAFIVDMTERRRLDDLVVQSEKMASVGGLAAGMAHEINNPLGGIMQGAQ
NIIRRLDPHREANRAAAQACGCPLEAIRAYLEQRGILRFIGGIEDSARRAARITSNMLEFSRPCAGEWREARLEHILDVA
LELAANDYDLKKRYDFRTIRIVRDYSPDVPAVRCIVSEVEQVALNLLRNAAQAMAEAATEAPAITLRTRVDGEYAVFEVE
DNGPGMSREVARRVFEPFFTTREPGRGTGLGLSVSYFIITATHKGAIAVQARPDRGTAFVVRLPLRSAECLHEEKPAVEN
>Mature_799_residues
TKSILCPGARMGIFQHPVTDDALYQAVQLPSFSYYMVASTRMQSVMELVQPLVVRTVILAAGLWVVGIFLTIVVYRHSVS
QYDLALSELQCTQRDELANLFKALVTDVQAAPEPATAMEVFLRRLCERTGFELGAVYLPDKQERYRFVIYQSDSFHPIAR
NPGKGMSAPDVLRAHLLGLMQHPVFLQAEQNVVVPDMSVDERFAEFYPAGEGSPAQPLLKTVIRGACVSRVRRASVGHVH
VGLFSLVSLEKSSDVAGLLDEVMPLLGPVAESKLAEVHLREQEMLFRNLYDRSALVQVVVNPETGTVLDANMAACEFYGY
PHEVFVTQTLYDLWQAPRDDIDAVMSASTELYTGLHIQKHILAGGDLKDVEAYMGRVPWKGQHALLCAVFDRSGIKKMEA
ALLRSEQQLAMLMEKLPLALALLDETGTIRQVNGAFVALTGRTAISCTGRYLHELGLESLHEASCPVVQDALLPAGREPQ
ELLLVTLQGEERHVLVHTTRLQMHEEGTRYAAFIVDMTERRRLDDLVVQSEKMASVGGLAAGMAHEINNPLGGIMQGAQN
IIRRLDPHREANRAAAQACGCPLEAIRAYLEQRGILRFIGGIEDSARRAARITSNMLEFSRPCAGEWREARLEHILDVAL
ELAANDYDLKKRYDFRTIRIVRDYSPDVPAVRCIVSEVEQVALNLLRNAAQAMAEAATEAPAITLRTRVDGEYAVFEVED
NGPGMSREVARRVFEPFFTTREPGRGTGLGLSVSYFIITATHKGAIAVQARPDRGTAFVVRLPLRSAECLHEEKPAVEN

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=305, Percent_Identity=29.5081967213115, Blast_Score=111, Evalue=2e-25,
Organism=Escherichia coli, GI1790300, Length=281, Percent_Identity=32.0284697508897, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1790436, Length=304, Percent_Identity=27.3026315789474, Blast_Score=92, Evalue=2e-19,
Organism=Escherichia coli, GI145693157, Length=319, Percent_Identity=26.3322884012539, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI87081816, Length=256, Percent_Identity=31.640625, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 88622; Mature: 88491

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50112 PAS ; PS00079 MULTICOPPER_OXIDASE1 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSILCPGARMGIFQHPVTDDALYQAVQLPSFSYYMVASTRMQSVMELVQPLVVRTVIL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AAGLWVVGIFLTIVVYRHSVSQYDLALSELQCTQRDELANLFKALVTDVQAAPEPATAME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
VFLRRLCERTGFELGAVYLPDKQERYRFVIYQSDSFHPIARNPGKGMSAPDVLRAHLLGL
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
MQHPVFLQAEQNVVVPDMSVDERFAEFYPAGEGSPAQPLLKTVIRGACVSRVRRASVGHV
HCCCEEEEECCCEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
HVGLFSLVSLEKSSDVAGLLDEVMPLLGPVAESKLAEVHLREQEMLFRNLYDRSALVQVV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VNPETGTVLDANMAACEFYGYPHEVFVTQTLYDLWQAPRDDIDAVMSASTELYTGLHIQK
ECCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCHHHH
HILAGGDLKDVEAYMGRVPWKGQHALLCAVFDRSGIKKMEAALLRSEQQLAMLMEKLPLA
HHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHCCHH
LALLDETGTIRQVNGAFVALTGRTAISCTGRYLHELGLESLHEASCPVVQDALLPAGREP
EEEECCCCCEEEECCEEEEEECCCEEEHHHHHHHHHCHHHHHHCCCCHHHHHHCCCCCCC
QELLLVTLQGEERHVLVHTTRLQMHEEGTRYAAFIVDMTERRRLDDLVVQSEKMASVGGL
CCEEEEEEECCCCEEEEEEEEHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AAGMAHEINNPLGGIMQGAQNIIRRLDPHREANRAAAQACGCPLEAIRAYLEQRGILRFI
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
GGIEDSARRAARITSNMLEFSRPCAGEWREARLEHILDVALELAANDYDLKKRYDFRTIR
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEE
IVRDYSPDVPAVRCIVSEVEQVALNLLRNAAQAMAEAATEAPAITLRTRVDGEYAVFEVE
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEE
DNGPGMSREVARRVFEPFFTTREPGRGTGLGLSVSYFIITATHKGAIAVQARPDRGTAFV
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCEEEEEECCCCCCEEE
VRLPLRSAECLHEEKPAVEN
EEECCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
TKSILCPGARMGIFQHPVTDDALYQAVQLPSFSYYMVASTRMQSVMELVQPLVVRTVIL
CCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHH
AAGLWVVGIFLTIVVYRHSVSQYDLALSELQCTQRDELANLFKALVTDVQAAPEPATAME
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHH
VFLRRLCERTGFELGAVYLPDKQERYRFVIYQSDSFHPIARNPGKGMSAPDVLRAHLLGL
HHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHH
MQHPVFLQAEQNVVVPDMSVDERFAEFYPAGEGSPAQPLLKTVIRGACVSRVRRASVGHV
HCCCEEEEECCCEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEE
HVGLFSLVSLEKSSDVAGLLDEVMPLLGPVAESKLAEVHLREQEMLFRNLYDRSALVQVV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
VNPETGTVLDANMAACEFYGYPHEVFVTQTLYDLWQAPRDDIDAVMSASTELYTGLHIQK
ECCCCCCEEECCHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHCCHHHH
HILAGGDLKDVEAYMGRVPWKGQHALLCAVFDRSGIKKMEAALLRSEQQLAMLMEKLPLA
HHHCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHCHHHHHHHHHHCCHH
LALLDETGTIRQVNGAFVALTGRTAISCTGRYLHELGLESLHEASCPVVQDALLPAGREP
EEEECCCCCEEEECCEEEEEECCCEEEHHHHHHHHHCHHHHHHCCCCHHHHHHCCCCCCC
QELLLVTLQGEERHVLVHTTRLQMHEEGTRYAAFIVDMTERRRLDDLVVQSEKMASVGGL
CCEEEEEEECCCCEEEEEEEEHHHHHCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AAGMAHEINNPLGGIMQGAQNIIRRLDPHREANRAAAQACGCPLEAIRAYLEQRGILRFI
HHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHH
GGIEDSARRAARITSNMLEFSRPCAGEWREARLEHILDVALELAANDYDLKKRYDFRTIR
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEE
IVRDYSPDVPAVRCIVSEVEQVALNLLRNAAQAMAEAATEAPAITLRTRVDGEYAVFEVE
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCEEEEEEE
DNGPGMSREVARRVFEPFFTTREPGRGTGLGLSVSYFIITATHKGAIAVQARPDRGTAFV
CCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCEEEEEECCCCCCEEE
VRLPLRSAECLHEEKPAVEN
EEECCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]