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The map label for this gene is fdhB [H]

Identifier: 78355765

GI number: 78355765

Start: 736966

End: 737679

Strand: Direct

Name: fdhB [H]

Synonym: Dde_0718

Alternate gene names: 78355765

Gene position: 736966-737679 (Clockwise)

Preceding gene: 78355764

Following gene: 78355766

Centisome position: 19.76

GC content: 57.42

Gene sequence:

>714_bases
ATGGGAAAGGCGTTCTTTGTTGATCTTACCCGCTGCACAGCCTGCCGCGGATGTCAGATAGCCTGCAAGCAGTGGCATAA
GCTGCCTGCCGAAGAAACCGTGAACTGGGGTTCGCATCAGAATCCCAGAGATCTGAGCTTCATATCGTACCGGCTGGTAC
GGTTCACCGAGGTTGTAAAGGACGGCAAACTTGACTGGCTGTTTTTTCCGGAACAGTGCCGTCACTGCTTTGAACCGCCC
TGCATGTATCAGGCAATGATTGATGACGAGCGGGCCATCCTGCGCGACGAAACCACGGGCGCGGTTATTTTTACCCGCCA
TACCAAAAACGTGGATGCCGCAGGAGTGCGTGCCGCATGCCCTTACGATATTCCCCGTGTGGATGAGGTAAGCGGTGTCA
TGTCCAAGTGCGACATGTGTCTGGACAGGGTGCATAACGGCATGAAGCCTGCCTGTGTGACTTCGTGCCCCACAGGTACC
ATGAACTTCGGTGACGAGGAGGAAATGCGCGAACTGGCCGAGGCCCGTCTGGCAGAAGTGCAGGGGCGCTACCCCGACGC
GGTGCTGGGTGACCCCGACGACGTTCGTGTCATCTACCTCTTCCAGCAGGACCCCATGGCCTACTTTGACAAGGCTGTGG
CCGAGCTTGGCCCTTCGCTGATGAACCGCAAACAGATGTTTGCCCGTCTGATGGGCCGCAAGGCACGTACCTGA

Upstream 100 bases:

>100_bases
GGCAAACCTGCTCACGCCGTCAGTGGGCGACCCCAATACCGGCATTCCCGAAACCAAGGCGTTCATGGTCGACGTCCGCA
AGCTGTAAGGAGGCTGAAAA

Downstream 100 bases:

>100_bases
TAACCGGCGTCTGCCGCACCGCCCGCAGGATGGTGCGGCAGACGCTGCATATACACGGCGGCGGGCAGACAGCCCGCCGT
GTATATGCAGCGTCTGCCGC

Product: formate dehydrogenase subunit beta

Products: CO2; NADH2 [C]

Alternate protein names: FDH subunit beta; Formate dehydrogenase small subunit [H]

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MGKAFFVDLTRCTACRGCQIACKQWHKLPAEETVNWGSHQNPRDLSFISYRLVRFTEVVKDGKLDWLFFPEQCRHCFEPP
CMYQAMIDDERAILRDETTGAVIFTRHTKNVDAAGVRAACPYDIPRVDEVSGVMSKCDMCLDRVHNGMKPACVTSCPTGT
MNFGDEEEMRELAEARLAEVQGRYPDAVLGDPDDVRVIYLFQQDPMAYFDKAVAELGPSLMNRKQMFARLMGRKART

Sequences:

>Translated_237_residues
MGKAFFVDLTRCTACRGCQIACKQWHKLPAEETVNWGSHQNPRDLSFISYRLVRFTEVVKDGKLDWLFFPEQCRHCFEPP
CMYQAMIDDERAILRDETTGAVIFTRHTKNVDAAGVRAACPYDIPRVDEVSGVMSKCDMCLDRVHNGMKPACVTSCPTGT
MNFGDEEEMRELAEARLAEVQGRYPDAVLGDPDDVRVIYLFQQDPMAYFDKAVAELGPSLMNRKQMFARLMGRKART
>Mature_236_residues
GKAFFVDLTRCTACRGCQIACKQWHKLPAEETVNWGSHQNPRDLSFISYRLVRFTEVVKDGKLDWLFFPEQCRHCFEPPC
MYQAMIDDERAILRDETTGAVIFTRHTKNVDAAGVRAACPYDIPRVDEVSGVMSKCDMCLDRVHNGMKPACVTSCPTGTM
NFGDEEEMRELAEARLAEVQGRYPDAVLGDPDDVRVIYLFQQDPMAYFDKAVAELGPSLMNRKQMFARLMGRKART

Specific function: Beta chain of the formate dehydrogenase (FDH) catalyze the reversible two-electron oxidation of formate to carbon dioxide. FDH loses activity in the presence of air, but this activity can be restored. This chain is an electron transfer unit [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790326, Length=204, Percent_Identity=36.2745098039216, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1787749, Length=205, Percent_Identity=36.5853658536585, Blast_Score=138, Evalue=3e-34,
Organism=Escherichia coli, GI1787122, Length=181, Percent_Identity=28.1767955801105, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789370, Length=207, Percent_Identity=28.0193236714976, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787872, Length=181, Percent_Identity=27.0718232044199, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI226510944, Length=170, Percent_Identity=27.6470588235294, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 26965; Mature: 26834

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.1 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
10.1 %Cys+Met (Translated Protein)
5.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
9.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKAFFVDLTRCTACRGCQIACKQWHKLPAEETVNWGSHQNPRDLSFISYRLVRFTEVVK
CCCEEEEEHHHHHCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
DGKLDWLFFPEQCRHCFEPPCMYQAMIDDERAILRDETTGAVIFTRHTKNVDAAGVRAAC
CCCEEEEECHHHHHHHCCCCHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
PYDIPRVDEVSGVMSKCDMCLDRVHNGMKPACVTSCPTGTMNFGDEEEMRELAEARLAEV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QGRYPDAVLGDPDDVRVIYLFQQDPMAYFDKAVAELGPSLMNRKQMFARLMGRKART
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GKAFFVDLTRCTACRGCQIACKQWHKLPAEETVNWGSHQNPRDLSFISYRLVRFTEVVK
CCEEEEEHHHHHCCCCCHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHH
DGKLDWLFFPEQCRHCFEPPCMYQAMIDDERAILRDETTGAVIFTRHTKNVDAAGVRAAC
CCCEEEEECHHHHHHHCCCCHHHHHHHCCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCC
PYDIPRVDEVSGVMSKCDMCLDRVHNGMKPACVTSCPTGTMNFGDEEEMRELAEARLAEV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
QGRYPDAVLGDPDDVRVIYLFQQDPMAYFDKAVAELGPSLMNRKQMFARLMGRKART
CCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD [C]

Specific reaction: formate + NAD = CO2 + NADH2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA