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The map label for this gene is hisJ [H]

Identifier: 78355442

GI number: 78355442

Start: 388729

End: 389532

Strand: Direct

Name: hisJ [H]

Synonym: Dde_0395

Alternate gene names: 78355442

Gene position: 388729-389532 (Clockwise)

Preceding gene: 78355438

Following gene: 78355443

Centisome position: 10.42

GC content: 60.2

Gene sequence:

>804_bases
ATGCAGACAGCCATCCGGAAGCTGAAGACTGTTCTGGTGCTGCTGTGCGCCGTGCTGACGGGGACAGCCGGCGTATGTGC
GGCACAGGGGCTGCCCGATCTCGCAGGGCGCACGGTGCATGCCGTGACCGGCAACGACTACCGGCCGCTCAATTTCATCG
ATCCGCAGACCGGCAACGGCATCGGGTTTGAGTATGATGCGGTGAATGAAATATGCCGTCGCCTCAACTGTACGGTGCAG
TGGCACGTCACATCCTGGGATACCATGATTTCCGCCGTGCGCGGCGGACAGTACGACGTGGGCATGGACGGCATAACCAT
CAATGATGAACGCCGAAAGCAGGTTGAGTTCACCGAACCCTACCTGACCAGTGAGCAGTTTATGCTGGTGCGCGCCGACG
AGCAGCGTTTTGCCGATGCGCAGACCTTCCGCGCCGACGAGCGGTTGCTCATTGGCTCGCAGCCGGGCACCACCAATTTT
TATGTGGCCGTGTACGATGTGCTGGACGGCGACGAGGCCAACCCCCGTATCAAGCTTTTTGAAACCTTCGGACCGGCCGT
GCAGGCCCTCATCCACGGCGATGTGGATATGGTGCTGATGGATGCCTCGTCCACACGCGGCTACATAGGCGCACGCCCCG
GCAAGCTCAAGGTTGTGGGTGGTCCGCTTGGTTCGGAGCAGTTCGGCTTTATCATGACGCCGGGCTCGGATTTTGTGGAA
CCGTTCAACGCCGCCATAGGCAGCATGAAGAAGGACGGCACGCTGGACGCTCTGAGCCGGCGGTGGTTTTACGAATACAG
GTAA

Upstream 100 bases:

>100_bases
TCCTGCGGTATGGCTTTGACATGCATGGCGGCACGGCATTGACAAGCATGGCGGCGCCCGCATACTTCATCAGACCATCA
ACGCGCAGGAGGACGCTTGT

Downstream 100 bases:

>100_bases
CGCAACGTGCAGCGGCCGGTTCTGCCCGGGCAAAGCCGTTCCGCTGTATCCGTAAGAGAACGATGTCATCATCCCGTACT
GCCGCAGGCCGCCGCGGGGG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: HBP; p29 [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MQTAIRKLKTVLVLLCAVLTGTAGVCAAQGLPDLAGRTVHAVTGNDYRPLNFIDPQTGNGIGFEYDAVNEICRRLNCTVQ
WHVTSWDTMISAVRGGQYDVGMDGITINDERRKQVEFTEPYLTSEQFMLVRADEQRFADAQTFRADERLLIGSQPGTTNF
YVAVYDVLDGDEANPRIKLFETFGPAVQALIHGDVDMVLMDASSTRGYIGARPGKLKVVGGPLGSEQFGFIMTPGSDFVE
PFNAAIGSMKKDGTLDALSRRWFYEYR

Sequences:

>Translated_267_residues
MQTAIRKLKTVLVLLCAVLTGTAGVCAAQGLPDLAGRTVHAVTGNDYRPLNFIDPQTGNGIGFEYDAVNEICRRLNCTVQ
WHVTSWDTMISAVRGGQYDVGMDGITINDERRKQVEFTEPYLTSEQFMLVRADEQRFADAQTFRADERLLIGSQPGTTNF
YVAVYDVLDGDEANPRIKLFETFGPAVQALIHGDVDMVLMDASSTRGYIGARPGKLKVVGGPLGSEQFGFIMTPGSDFVE
PFNAAIGSMKKDGTLDALSRRWFYEYR
>Mature_267_residues
MQTAIRKLKTVLVLLCAVLTGTAGVCAAQGLPDLAGRTVHAVTGNDYRPLNFIDPQTGNGIGFEYDAVNEICRRLNCTVQ
WHVTSWDTMISAVRGGQYDVGMDGITINDERRKQVEFTEPYLTSEQFMLVRADEQRFADAQTFRADERLLIGSQPGTTNF
YVAVYDVLDGDEANPRIKLFETFGPAVQALIHGDVDMVLMDASSTRGYIGARPGKLKVVGGPLGSEQFGFIMTPGSDFVE
PFNAAIGSMKKDGTLDALSRRWFYEYR

Specific function: Component of the high-affinity histidine permease, a binding-protein-dependent transport system. The other components are proteins hisQ, hisM, and hisP [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=219, Percent_Identity=26.4840182648402, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1787031, Length=258, Percent_Identity=24.4186046511628, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787085, Length=213, Percent_Identity=25.3521126760563, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787088, Length=249, Percent_Identity=23.2931726907631, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788649, Length=278, Percent_Identity=23.3812949640288, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29455; Mature: 29455

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTAIRKLKTVLVLLCAVLTGTAGVCAAQGLPDLAGRTVHAVTGNDYRPLNFIDPQTGNG
CCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
IGFEYDAVNEICRRLNCTVQWHVTSWDTMISAVRGGQYDVGMDGITINDERRKQVEFTEP
CCEEHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEECCCCCEEECCHHHCCEEECCC
YLTSEQFMLVRADEQRFADAQTFRADERLLIGSQPGTTNFYVAVYDVLDGDEANPRIKLF
CCCCCEEEEEEECHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCEEEE
ETFGPAVQALIHGDVDMVLMDASSTRGYIGARPGKLKVVGGPLGSEQFGFIMTPGSDFVE
EHHCHHHHHHHCCCEEEEEEECCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCHHHHH
PFNAAIGSMKKDGTLDALSRRWFYEYR
HHHHHHCCCCCCCCHHHHHHCEEECCC
>Mature Secondary Structure
MQTAIRKLKTVLVLLCAVLTGTAGVCAAQGLPDLAGRTVHAVTGNDYRPLNFIDPQTGNG
CCHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCC
IGFEYDAVNEICRRLNCTVQWHVTSWDTMISAVRGGQYDVGMDGITINDERRKQVEFTEP
CCEEHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCCEECCCCCEEECCHHHCCEEECCC
YLTSEQFMLVRADEQRFADAQTFRADERLLIGSQPGTTNFYVAVYDVLDGDEANPRIKLF
CCCCCEEEEEEECHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCEEEE
ETFGPAVQALIHGDVDMVLMDASSTRGYIGARPGKLKVVGGPLGSEQFGFIMTPGSDFVE
EHHCHHHHHHHCCCEEEEEEECCCCCCEEECCCCEEEEEECCCCCCCCEEEECCCHHHHH
PFNAAIGSMKKDGTLDALSRRWFYEYR
HHHHHHCCCCCCCCHHHHHHCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9192026; 9395059; 10688204 [H]