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The map label for this gene is epsM [H]

Identifier: 78355406

GI number: 78355406

Start: 347992

End: 348633

Strand: Direct

Name: epsM [H]

Synonym: Dde_0359

Alternate gene names: 78355406

Gene position: 347992-348633 (Clockwise)

Preceding gene: 78355404

Following gene: 78355412

Centisome position: 9.33

GC content: 64.8

Gene sequence:

>642_bases
ATGGATTCACTTGTTCTTTTTGGTGCGGGCGGCCACGGCAAAGTGCTGCTTGATGCGGCGCGTGCCGGCGGCGGGCGTGT
GCTTTTCTGCGCGGACGATGATGCGGCACGTGCCGGAACGGTTTTCTGCGGTCTGGAAGTGAAACCGGCCGCGCAGCTGG
GCAGTTTTTGCAATTGCGGCAACGTCGCACTGCTGAACGGCATCGGGTATGTGGGCGGCCGCAATGTGCGGGCACATGTC
CATGCCCGTCTGACAGCCGGGGGCTGGCGTTTTGCGACGGTGTGTCATCCGGCGGCCGTCATAGCGCAGGATGCCGTGCT
GGCGGATGGCGTGCAGGTGCTTGCAGGCGCTGTGGTGGCGCCGTCGGTCAGAGTGGGGGTGAATTCCATCATTAATCACC
GTGCGGTTGTCGACCATGATTGCGTGGTGGGCGAACATTGCCATGTGGCGCCCGGAGTCACGCTGTGCGGCGGGGTGCGG
CTGGGCAGCGGTGTTTTTGTGGGAGCCGGAGCCACCGTTGTTCCGGGGGTCAGCATAGGCGACGGTGCGGTCATAGGCGC
GGGGGCCACTGTTCTGCGCCATGTGTCCGCAGGCAGTGTGGTGGCGGGCACTCCGGCCATACCCCTTGCGGGCAAAGTGT
GA

Upstream 100 bases:

>100_bases
TGAGCGCCGGAGGGAAGCACGGTCTTGTTCCGGCGACTGATACACCATTACGGGCGCTGCGTCCATTCACTGCATTTTCT
GTACTGGCGGTTGGATTATC

Downstream 100 bases:

>100_bases
TATTTTTTTATATTTCATGATCATAAAAAAGCGGCCCCGGCGTCATGCCGGGGCCGTTTGTTTTATCTGCGGGGCAGCGA
ACGTCACGCAGGTTCCGGCC

Product: hexapeptide transferase family protein

Products: CoA; acetylmaltose [C]

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MDSLVLFGAGGHGKVLLDAARAGGGRVLFCADDDAARAGTVFCGLEVKPAAQLGSFCNCGNVALLNGIGYVGGRNVRAHV
HARLTAGGWRFATVCHPAAVIAQDAVLADGVQVLAGAVVAPSVRVGVNSIINHRAVVDHDCVVGEHCHVAPGVTLCGGVR
LGSGVFVGAGATVVPGVSIGDGAVIGAGATVLRHVSAGSVVAGTPAIPLAGKV

Sequences:

>Translated_213_residues
MDSLVLFGAGGHGKVLLDAARAGGGRVLFCADDDAARAGTVFCGLEVKPAAQLGSFCNCGNVALLNGIGYVGGRNVRAHV
HARLTAGGWRFATVCHPAAVIAQDAVLADGVQVLAGAVVAPSVRVGVNSIINHRAVVDHDCVVGEHCHVAPGVTLCGGVR
LGSGVFVGAGATVVPGVSIGDGAVIGAGATVLRHVSAGSVVAGTPAIPLAGKV
>Mature_213_residues
MDSLVLFGAGGHGKVLLDAARAGGGRVLFCADDDAARAGTVFCGLEVKPAAQLGSFCNCGNVALLNGIGYVGGRNVRAHV
HARLTAGGWRFATVCHPAAVIAQDAVLADGVQVLAGAVVAPSVRVGVNSIINHRAVVDHDCVVGEHCHVAPGVTLCGGVR
LGSGVFVGAGATVVPGVSIGDGAVIGAGATVLRHVSAGSVVAGTPAIPLAGKV

Specific function: May be involved in the production of the exopolysaccharide (EPS) component of the extracellular matrix during biofilm formation. EPS is responsible for the adhesion of chains of cells into bundles [H]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018357
- InterPro:   IPR013817
- InterPro:   IPR020019
- InterPro:   IPR011004 [H]

Pfam domain/function: NA

EC number: 2.3.1.79 [C]

Molecular weight: Translated: 20847; Mature: 20847

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSLVLFGAGGHGKVLLDAARAGGGRVLFCADDDAARAGTVFCGLEVKPAAQLGSFCNCG
CCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCHHHHCCCCCCC
NVALLNGIGYVGGRNVRAHVHARLTAGGWRFATVCHPAAVIAQDAVLADGVQVLAGAVVA
CEEEEECCCCCCCCCEEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHC
PSVRVGVNSIINHRAVVDHDCVVGEHCHVAPGVTLCGGVRLGSGVFVGAGATVVPGVSIG
CCHHCCHHHHHCCCEEECCCCEECCCCCCCCCCEEECCEEECCCEEEECCCEEECCCCCC
DGAVIGAGATVLRHVSAGSVVAGTPAIPLAGKV
CCEEEECCHHHHHCCCCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MDSLVLFGAGGHGKVLLDAARAGGGRVLFCADDDAARAGTVFCGLEVKPAAQLGSFCNCG
CCCEEEEECCCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEECCHHHHCCCCCCC
NVALLNGIGYVGGRNVRAHVHARLTAGGWRFATVCHPAAVIAQDAVLADGVQVLAGAVVA
CEEEEECCCCCCCCCEEEEEEEEEECCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHC
PSVRVGVNSIINHRAVVDHDCVVGEHCHVAPGVTLCGGVRLGSGVFVGAGATVVPGVSIG
CCHHCCHHHHHCCCEEECCCCEECCCCCCCCCCEEECCEEECCCEEEECCCEEECCCCCC
DGAVIGAGATVLRHVSAGSVVAGTPAIPLAGKV
CCEEEECCHHHHHCCCCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 0.20 {glucose} 0.11 {maltose}

Km value (mM): 0.018 {acetyl-CoA}} 62 {glucose}} 40 {triethanolamine}} 90 {maltose}} [C]

Substrates: Acetyl-CoA; maltose [C]

Specific reaction: Acetyl-CoA + maltose = CoA + acetylmaltose [C]

General reaction: Acyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969506; 9384377 [H]