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The map label for this gene is rsmF [H]

Identifier: 78355169

GI number: 78355169

Start: 114525

End: 115814

Strand: Direct

Name: rsmF [H]

Synonym: Dde_0122

Alternate gene names: 78355169

Gene position: 114525-115814 (Clockwise)

Preceding gene: 78355166

Following gene: 78355171

Centisome position: 3.07

GC content: 62.64

Gene sequence:

>1290_bases
ATGGCAGAATCCCCTCAGGCGCGGTCGTTCCGCGTAGTATGTCCTGCCGGACAGGAATCGCTGGTTGAAGCCCTGCTGCA
CAGTCAGGGATACGAGTTTGAACCGGAGCCGGTATCGCCCTGGACCCGCAGGCTTACAAAGGAGCCCAGACCGCTGGGCA
GCAGTCTGGCAGCTTTTTTCGGACTGATTTACATTCAGGACAGATCTTCCATGCTGCCGCCTCTGGCGCTGGCGCCGCAG
AACGGCGATGCCGTGCTGGACATGTGTGCAAGTCCGGGCAGCAAAACGGGCTTTCTGGCGCAGCTGGCAGGGCCGGACGG
TTTTGTGATGGGTAACGAACCCAACGCGCAGCGGCTGGCAACCCTGCGTCAGAATCTGTTCAACCTGAACCTGCCGCATA
CGGCCACCTGCGGCTACGCCGGAGAATCCCTGCCCCTGCCGGATGCATCATGGCAGAAAATCCAGCTGGACCCGCCATGC
AGCGGCTGGGGCACCGTGGATAAAAACCCCAACGTGATGAAGCTGTGGCAGGGGGATAAAGTCAAGCCGCTTGTGGGCAT
CCAGCGGCTGCTGCTCAGAGAGGCTGCGCGGCTGCTGGCTCCGGGCGGGGTTGTGGTTTATTCCACCTGCACGACCAACG
TGCAGGAAAATGAAGAACAGGTGCGCTGGGCAACGCAGGAACTGGGGCTGATCCCCGAACCGCTGGCACCGTTTGAAGGT
TTTGTTTTTGAAGACCCGCTGCTGCCGGGGTGCGAGGGAACGCTGCGGGTGGATGCATCCCGTTCATCGGCACAGGGATT
TTATATCGCGCGTTTCCGCAAAGAGGGCGATTCACCGCCGCCTGAACCTGTTTCTGCCATGCGGGTGCAGGGAATACACA
GACAGGCATTGCGCGGACCGTGTGCAGATCCGGACATGCTGCCGGACGGCGAGGTGGCTCTGTTCGGTTCCGCGGCCATG
TTTCTGCCGCAACAGGCCCTGAACTTGCTGCCCGAATCCTTCCGCTGGCGGGGATACCCGCTAGGCAAGGCTTCGCGCGG
CGAGGTGAGGGTCTCACCCCGGATGCGCTGCATGATGCCCCCCGCAGATCAGTGCGCCGCAGGCGCGTTGAATGTGGAAG
AGCCGGAACTGATAAGCGGCCTGCTGTCCGGCCAGAGCATTGATCTGGCCCGCGGTGCGTATGCGGGCAGCGTGGATGCA
TCCCTGCCGGAGGCCGGACTGTATTTCAGAGGGCTGCCGCTGGGCAGGCTGCGTATCAAGGGGCGCCGTGCCTTGTGGGC
GGAGCGCTGA

Upstream 100 bases:

>100_bases
TGCATCGGCAGCGGACACGCTGCCGTGACTGTCCTCTCATAATACCTCTTGCTGATGCAAGCAAGCTTAGGGTAGACGCG
CAGCAAAAACAGGAGTGCAA

Downstream 100 bases:

>100_bases
TCATCCCCGCGCTATGAAAAGATCGTTGAGCAGAGAGCTTTTGCCGTATCTGAGGGGGCGGCCGTCCATATCGGTCACAG
TGCCGCCCGCTCCGCGCAGA

Product: NOL1/NOP2/sun family protein

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MAESPQARSFRVVCPAGQESLVEALLHSQGYEFEPEPVSPWTRRLTKEPRPLGSSLAAFFGLIYIQDRSSMLPPLALAPQ
NGDAVLDMCASPGSKTGFLAQLAGPDGFVMGNEPNAQRLATLRQNLFNLNLPHTATCGYAGESLPLPDASWQKIQLDPPC
SGWGTVDKNPNVMKLWQGDKVKPLVGIQRLLLREAARLLAPGGVVVYSTCTTNVQENEEQVRWATQELGLIPEPLAPFEG
FVFEDPLLPGCEGTLRVDASRSSAQGFYIARFRKEGDSPPPEPVSAMRVQGIHRQALRGPCADPDMLPDGEVALFGSAAM
FLPQQALNLLPESFRWRGYPLGKASRGEVRVSPRMRCMMPPADQCAAGALNVEEPELISGLLSGQSIDLARGAYAGSVDA
SLPEAGLYFRGLPLGRLRIKGRRALWAER

Sequences:

>Translated_429_residues
MAESPQARSFRVVCPAGQESLVEALLHSQGYEFEPEPVSPWTRRLTKEPRPLGSSLAAFFGLIYIQDRSSMLPPLALAPQ
NGDAVLDMCASPGSKTGFLAQLAGPDGFVMGNEPNAQRLATLRQNLFNLNLPHTATCGYAGESLPLPDASWQKIQLDPPC
SGWGTVDKNPNVMKLWQGDKVKPLVGIQRLLLREAARLLAPGGVVVYSTCTTNVQENEEQVRWATQELGLIPEPLAPFEG
FVFEDPLLPGCEGTLRVDASRSSAQGFYIARFRKEGDSPPPEPVSAMRVQGIHRQALRGPCADPDMLPDGEVALFGSAAM
FLPQQALNLLPESFRWRGYPLGKASRGEVRVSPRMRCMMPPADQCAAGALNVEEPELISGLLSGQSIDLARGAYAGSVDA
SLPEAGLYFRGLPLGRLRIKGRRALWAER
>Mature_428_residues
AESPQARSFRVVCPAGQESLVEALLHSQGYEFEPEPVSPWTRRLTKEPRPLGSSLAAFFGLIYIQDRSSMLPPLALAPQN
GDAVLDMCASPGSKTGFLAQLAGPDGFVMGNEPNAQRLATLRQNLFNLNLPHTATCGYAGESLPLPDASWQKIQLDPPCS
GWGTVDKNPNVMKLWQGDKVKPLVGIQRLLLREAARLLAPGGVVVYSTCTTNVQENEEQVRWATQELGLIPEPLAPFEGF
VFEDPLLPGCEGTLRVDASRSSAQGFYIARFRKEGDSPPPEPVSAMRVQGIHRQALRGPCADPDMLPDGEVALFGSAAMF
LPQQALNLLPESFRWRGYPLGKASRGEVRVSPRMRCMMPPADQCAAGALNVEEPELISGLLSGQSIDLARGAYAGSVDAS
LPEAGLYFRGLPLGRLRIKGRRALWAER

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=257, Percent_Identity=32.6848249027237, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI76150623, Length=257, Percent_Identity=32.6848249027237, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI32698918, Length=175, Percent_Identity=34.8571428571429, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI301336155, Length=220, Percent_Identity=31.8181818181818, Blast_Score=92, Evalue=9e-19,
Organism=Homo sapiens, GI39995082, Length=174, Percent_Identity=34.4827586206897, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI11545785, Length=227, Percent_Identity=31.2775330396476, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI23199998, Length=230, Percent_Identity=31.304347826087, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI270288816, Length=230, Percent_Identity=31.304347826087, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI8922322, Length=230, Percent_Identity=31.304347826087, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI270288818, Length=230, Percent_Identity=31.304347826087, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI87081985, Length=247, Percent_Identity=34.8178137651822, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI2367212, Length=206, Percent_Identity=30.5825242718447, Blast_Score=86, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536757, Length=252, Percent_Identity=32.5396825396825, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI25143471, Length=210, Percent_Identity=31.9047619047619, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI71998419, Length=233, Percent_Identity=27.8969957081545, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115534021, Length=234, Percent_Identity=28.6324786324786, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324268, Length=258, Percent_Identity=27.906976744186, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319447, Length=185, Percent_Identity=35.6756756756757, Blast_Score=92, Evalue=2e-19,
Organism=Drosophila melanogaster, GI22024126, Length=257, Percent_Identity=29.1828793774319, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI21355201, Length=176, Percent_Identity=38.6363636363636, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24668781, Length=239, Percent_Identity=32.2175732217573, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI21356513, Length=237, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19920494, Length=210, Percent_Identity=29.0476190476191, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 46540; Mature: 46409

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAESPQARSFRVVCPAGQESLVEALLHSQGYEFEPEPVSPWTRRLTKEPRPLGSSLAAFF
CCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
GLIYIQDRSSMLPPLALAPQNGDAVLDMCASPGSKTGFLAQLAGPDGFVMGNEPNAQRLA
EEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCHHHHH
TLRQNLFNLNLPHTATCGYAGESLPLPDASWQKIQLDPPCSGWGTVDKNPNVMKLWQGDK
HHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCC
VKPLVGIQRLLLREAARLLAPGGVVVYSTCTTNVQENEEQVRWATQELGLIPEPLAPFEG
CCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
FVFEDPLLPGCEGTLRVDASRSSAQGFYIARFRKEGDSPPPEPVSAMRVQGIHRQALRGP
CEECCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
CADPDMLPDGEVALFGSAAMFLPQQALNLLPESFRWRGYPLGKASRGEVRVSPRMRCMMP
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEECCCEEEECC
PADQCAAGALNVEEPELISGLLSGQSIDLARGAYAGSVDASLPEAGLYFRGLPLGRLRIK
CHHHHHCCCCCCCCHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCEEECCCCCEEEEC
GRRALWAER
CCCCCCCCC
>Mature Secondary Structure 
AESPQARSFRVVCPAGQESLVEALLHSQGYEFEPEPVSPWTRRLTKEPRPLGSSLAAFF
CCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCHHHHHHHH
GLIYIQDRSSMLPPLALAPQNGDAVLDMCASPGSKTGFLAQLAGPDGFVMGNEPNAQRLA
EEEEEECCCCCCCCEEECCCCCCHHHHHHCCCCCCCCEEEEECCCCCEEECCCCCHHHHH
TLRQNLFNLNLPHTATCGYAGESLPLPDASWQKIQLDPPCSGWGTVDKNPNVMKLWQGDK
HHHHHHHCCCCCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEECCCCC
VKPLVGIQRLLLREAARLLAPGGVVVYSTCTTNVQENEEQVRWATQELGLIPEPLAPFEG
CCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
FVFEDPLLPGCEGTLRVDASRSSAQGFYIARFRKEGDSPPPEPVSAMRVQGIHRQALRGP
CEECCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
CADPDMLPDGEVALFGSAAMFLPQQALNLLPESFRWRGYPLGKASRGEVRVSPRMRCMMP
CCCCCCCCCCCEEEECCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCEEECCCEEEECC
PADQCAAGALNVEEPELISGLLSGQSIDLARGAYAGSVDASLPEAGLYFRGLPLGRLRIK
CHHHHHCCCCCCCCHHHHHHHHCCCCEEHHCCCCCCCCCCCCCCCCCEEECCCCCEEEEC
GRRALWAER
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA