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The map label for this gene is hydA [H]

Identifier: 78355128

GI number: 78355128

Start: 75821

End: 77086

Strand: Direct

Name: hydA [H]

Synonym: Dde_0081

Alternate gene names: 78355128

Gene position: 75821-77086 (Clockwise)

Preceding gene: 78355126

Following gene: 78355129

Centisome position: 2.03

GC content: 52.45

Gene sequence:

>1266_bases
ATGAACTTGGTTGAAATGGAAAAGATTCAGTATGTGGATCAGTCGCCTGACCCGCGAGCGAATCCGGATGAACTTTTTTT
CATCCAGATTGATCCGGAAAAATGTATCGGCTGCGATACCTGTCAGGAGTATTGCCCGACGGGCGCCATATTTGGCGATA
CCGGGAGTGCGCATAGTATTCCTCATGAAGAAATCTGCATCAATTGCGGGCAGTGTCTTACTCATTGCCCTGTCGGAGCC
ATTTACGAGGTGCAGTCCTGGGTTCGGGAGCTGAGTGAAAAAATCAAAGATCCGGAGATCAAGGTAATCGCCATGCCGGC
TCCTGCTGTCCGGTATGGGCTTGGAGAATGTTTCGGCATGCCGGTGGGCACGGTAACCACGGGAAAGATGCTGACAGCTT
TGCAGATGCTGGGCTTTGACCACGTATGGGACAATGAGTTCACCGCAGATGTCACCATATGGGAAGAAGGCACCGAGTTT
GTCAAACGCCTTACAGGGCAGATTGACAAGCCGCTTCCTCAGTTTACCTCTTGCTGCCCCGGCTGGCACAAATATGTGGA
AAGCTTTTATCCTGAGTTGTTCCCGCATCTTTCAAGCTGCAAATCTCCCATCGGGATGATGGGCGCACTGGCCAAAACGT
ATGGACCGGATGTGATGAAATATGATCGGTCCAAGGTTTATACGGTTTCCATTATGCCCTGTACCGCTAAAAAGTATGAG
GGCATGCGCGCGGACCTGTGGTCCAGCGGCTACAAAGACATTGACGCAACCATAGACACCCGCGAACTGGCCTACATGAT
AAAAAAGGCAGGCATTGATTTCGCCGCTCTGCCGGACGGCAAACGGGACACGTTGATGGGTGATTCCACCGGTGGGGCCA
CGATTTTCGGTGTATCCGGCGGTGTTATGGAAGCGGCTTTGCGTTATGCCTACGAGGCTGTGACCGGCAAAAAACCGTCG
AGCTGGGATTTTACGATGGTTCGCGGCCTGAACGGTATCAAAGAAGGTACTGTCACCATTGGCGATGCAAAGATAAACGT
GGCGGTGGTTCACGGAGCAAAGCGTTTTGCGGAAGTATGCGAGGTGATTAAGACAGGGAAAAGCCCGTGGCATTTCATCG
AGTTTATGGCCTGCCCCGGAGGATGTGTCTGCGGTGGCGGTCAGCCTGTGATGCCCGGAGTACTGGAAGCTATGGACCGC
AAAGTTTCCAGAACCTTTGCCGGACTCAAAGAGCGTCTGAACAGAATGTCGTCCAGCAAGGCATAG

Upstream 100 bases:

>100_bases
TATTCACATAATATCATGAATTCGCATGCTCTAGGTTCCAATGGCATTCTTCGGCTTTTGGTACGCCGTGGAAATCCCCT
TATAATAGACGGAGGTAGCT

Downstream 100 bases:

>100_bases
GAGGATGAAATGAGTATTGCTGCATTCACCCGTCGTCAGTTTCTGAAAGCCGGCTGCATGGCCTGCGGCGCTGCAATTGT
TGGTATCCGCTTTACCGGAA

Product: ferredoxin hydrogenase

Products: NA

Alternate protein names: Fe hydrogenlyase [H]

Number of amino acids: Translated: 421; Mature: 421

Protein sequence:

>421_residues
MNLVEMEKIQYVDQSPDPRANPDELFFIQIDPEKCIGCDTCQEYCPTGAIFGDTGSAHSIPHEEICINCGQCLTHCPVGA
IYEVQSWVRELSEKIKDPEIKVIAMPAPAVRYGLGECFGMPVGTVTTGKMLTALQMLGFDHVWDNEFTADVTIWEEGTEF
VKRLTGQIDKPLPQFTSCCPGWHKYVESFYPELFPHLSSCKSPIGMMGALAKTYGPDVMKYDRSKVYTVSIMPCTAKKYE
GMRADLWSSGYKDIDATIDTRELAYMIKKAGIDFAALPDGKRDTLMGDSTGGATIFGVSGGVMEAALRYAYEAVTGKKPS
SWDFTMVRGLNGIKEGTVTIGDAKINVAVVHGAKRFAEVCEVIKTGKSPWHFIEFMACPGGCVCGGGQPVMPGVLEAMDR
KVSRTFAGLKERLNRMSSSKA

Sequences:

>Translated_421_residues
MNLVEMEKIQYVDQSPDPRANPDELFFIQIDPEKCIGCDTCQEYCPTGAIFGDTGSAHSIPHEEICINCGQCLTHCPVGA
IYEVQSWVRELSEKIKDPEIKVIAMPAPAVRYGLGECFGMPVGTVTTGKMLTALQMLGFDHVWDNEFTADVTIWEEGTEF
VKRLTGQIDKPLPQFTSCCPGWHKYVESFYPELFPHLSSCKSPIGMMGALAKTYGPDVMKYDRSKVYTVSIMPCTAKKYE
GMRADLWSSGYKDIDATIDTRELAYMIKKAGIDFAALPDGKRDTLMGDSTGGATIFGVSGGVMEAALRYAYEAVTGKKPS
SWDFTMVRGLNGIKEGTVTIGDAKINVAVVHGAKRFAEVCEVIKTGKSPWHFIEFMACPGGCVCGGGQPVMPGVLEAMDR
KVSRTFAGLKERLNRMSSSKA
>Mature_421_residues
MNLVEMEKIQYVDQSPDPRANPDELFFIQIDPEKCIGCDTCQEYCPTGAIFGDTGSAHSIPHEEICINCGQCLTHCPVGA
IYEVQSWVRELSEKIKDPEIKVIAMPAPAVRYGLGECFGMPVGTVTTGKMLTALQMLGFDHVWDNEFTADVTIWEEGTEF
VKRLTGQIDKPLPQFTSCCPGWHKYVESFYPELFPHLSSCKSPIGMMGALAKTYGPDVMKYDRSKVYTVSIMPCTAKKYE
GMRADLWSSGYKDIDATIDTRELAYMIKKAGIDFAALPDGKRDTLMGDSTGGATIFGVSGGVMEAALRYAYEAVTGKKPS
SWDFTMVRGLNGIKEGTVTIGDAKINVAVVHGAKRFAEVCEVIKTGKSPWHFIEFMACPGGCVCGGGQPVMPGVLEAMDR
KVSRTFAGLKERLNRMSSSKA

Specific function: May be involved in hydrogen uptake for the reduction of sulfate to hydrogen sulfide in an electron transport chain. Cytochrome c3 is likely to be the physiological electron carrier for the enzyme [H]

COG id: COG4624

COG function: function code R; Iron only hydrogenase large subunit, C-terminal domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI11968051, Length=371, Percent_Identity=29.1105121293801, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI6912524, Length=359, Percent_Identity=26.1838440111421, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI134284357, Length=283, Percent_Identity=27.5618374558304, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI84452151, Length=287, Percent_Identity=27.8745644599303, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI14165461, Length=405, Percent_Identity=23.7037037037037, Blast_Score=103, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71995015, Length=348, Percent_Identity=25.8620689655172, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI116007470, Length=364, Percent_Identity=24.4505494505494, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007466, Length=364, Percent_Identity=24.4505494505494, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007464, Length=364, Percent_Identity=24.4505494505494, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007468, Length=364, Percent_Identity=24.4505494505494, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI116007462, Length=246, Percent_Identity=26.4227642276423, Blast_Score=100, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009016
- InterPro:   IPR004108
- InterPro:   IPR013352 [H]

Pfam domain/function: PF02906 Fe_hyd_lg_C; PF00037 Fer4 [H]

EC number: =1.12.7.2 [H]

Molecular weight: Translated: 46135; Mature: 46135

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
8.6 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
8.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLVEMEKIQYVDQSPDPRANPDELFFIQIDPEKCIGCDTCQEYCPTGAIFGDTGSAHSI
CCCHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHCCCHHHHHHCCCCCEECCCCCCCCC
PHEEICINCGQCLTHCPVGAIYEVQSWVRELSEKIKDPEIKVIAMPAPAVRYGLGECFGM
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCC
PVGTVTTGKMLTALQMLGFDHVWDNEFTADVTIWEEGTEFVKRLTGQIDKPLPQFTSCCP
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCC
GWHKYVESFYPELFPHLSSCKSPIGMMGALAKTYGPDVMKYDRSKVYTVSIMPCTAKKYE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCCCEEEEEEEEECCHHHHC
GMRADLWSSGYKDIDATIDTRELAYMIKKAGIDFAALPDGKRDTLMGDSTGGATIFGVSG
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEECCC
GVMEAALRYAYEAVTGKKPSSWDFTMVRGLNGIKEGTVTIGDAKINVAVVHGAKRFAEVC
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEECCEEEEEEECCHHHHHHHH
EVIKTGKSPWHFIEFMACPGGCVCGGGQPVMPGVLEAMDRKVSRTFAGLKERLNRMSSSK
HHHHCCCCCHHHHEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
A
C
>Mature Secondary Structure
MNLVEMEKIQYVDQSPDPRANPDELFFIQIDPEKCIGCDTCQEYCPTGAIFGDTGSAHSI
CCCHHHHHHHHCCCCCCCCCCCCCEEEEEECHHHHCCCHHHHHHCCCCCEECCCCCCCCC
PHEEICINCGQCLTHCPVGAIYEVQSWVRELSEKIKDPEIKVIAMPAPAVRYGLGECFGM
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHCC
PVGTVTTGKMLTALQMLGFDHVWDNEFTADVTIWEEGTEFVKRLTGQIDKPLPQFTSCCP
CCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCCCCCHHHHHCCC
GWHKYVESFYPELFPHLSSCKSPIGMMGALAKTYGPDVMKYDRSKVYTVSIMPCTAKKYE
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHCCCCEEEEEEEEECCHHHHC
GMRADLWSSGYKDIDATIDTRELAYMIKKAGIDFAALPDGKRDTLMGDSTGGATIFGVSG
CCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEECCC
GVMEAALRYAYEAVTGKKPSSWDFTMVRGLNGIKEGTVTIGDAKINVAVVHGAKRFAEVC
HHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCEEEECCEEEEEEECCHHHHHHHH
EVIKTGKSPWHFIEFMACPGGCVCGGGQPVMPGVLEAMDRKVSRTFAGLKERLNRMSSSK
HHHHCCCCCHHHHEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2661538 [H]