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The map label for this gene is yneF [H]

Identifier: 78355124

GI number: 78355124

Start: 66609

End: 67844

Strand: Direct

Name: yneF [H]

Synonym: Dde_0077

Alternate gene names: 78355124

Gene position: 66609-67844 (Clockwise)

Preceding gene: 78355119

Following gene: 78355126

Centisome position: 1.79

GC content: 61.73

Gene sequence:

>1236_bases
ATGGTGAATGCTGCCGCGGTGGGCAGGCTGCTGGCGGTGCCGCTGGGACTATTTGTGGCGGCGGCTCTGGCCGGTCTGTT
TATGAATACGCTGCCGCCTTCCGCCGGAGAGGTGGCCCCCTATGCGGCGTGGGCGGCTCTGGCCGGGGGAATGCCTCTGG
CGGAGCGCTTCAGAAGAAGCCGCGCCGTTTTTGTTCTGCTGCTGGGGTGCGGCGTGTTTCTGTTGCTGTACAATGTGCTT
CCGGCGCTGGGCGACGCCACCCGCGTGCGGCTGTGGTACGGCATTGTGCTTACCCTGCTGCCCTGCAATCTGCTGCTGTT
TGCCTTTCTGGAAGACAGAGGGGTTTTTTCGCGCTGGGGCATGGGCATGCTTGCATGGCTGCTGGGGCAGTTCATGACGG
CGGCCTTTATTGTGCGCGGCGGCGGTGTGCTGCTGGCGACATCGCTGGGCAGAACCATGCAGCGCGCTGTGGAGGGCTGG
GTTTATGCCTCTCCCCTGCCCGGCTGGTTCGACAGCTGGACTCCGCTCACGCAGCCTGCCCTGCTGCTTGTGCTGCTTGC
TGCCGTGGTGCTTGTGGTGCGTGTCGTCCGGCTGGAAGACGGCTTCAGTGCAAGTCTGCTGGGCACTCTGCTCAGCCTGA
CAGCCGCGTTTCACGCTGTGGGCAACGGGCCGGTGGTCAGCCTGTTTGTGGCCGCGGCCGGTGCCGCCCAGCTGACAGCA
TTATTCCGCGATTCCTACAGCATGGCTTTTGTGGACGAGCTAACCGAGGTGCCGTCGCGGCGTGCGCTGATGGCGGATGC
GAAAAAGCTGGGCGGAAGCTATTGCGTGGCCATGGCCGATGTGGATCATTTCAAAGCTTTTAATGATACATACGGCCACG
ATGTGGGCGATGACGTGCTGCGTATGGTGGCTGCGCGGCTGGCCGGAGTGGACGGCGGCAAGGTGTACCGTTATGGCGGC
GAAGAGTTCACCATTCTGTTTCCACGTCTGGGCAGAGCCGAAGCCAGACCCCATCTTGAAGCCGTGCGGGAGGCTGTGGC
AGCCATACCTTTCCGCATCCGTACCCGTAACAAAAGTGCCAAGGTGGTGCGCGATAAGTCAGTCACCGTGACTGTGAGCA
TCGGGCTGGCGCAGCGCGAAGACGGCGAAGACGACTTTGAATCGGTGCTGAAAAAAGCGGATAAAGCCTTATACAAGGCC
AAAAAGGCAGGCAGAAACAAAGTCGCTCTGCTGTGA

Upstream 100 bases:

>100_bases
CTATTATGTTCCGGCAACTGATAAAGGAAAACGGCATGATGCATATATATGGAAAAGATGCGCCCCCCCGCCGGCACCAA
AGCGCCGCGGCGGAGGCTGC

Downstream 100 bases:

>100_bases
ACGGCCCTGTCCTGCCTGTCTGTCCTGTCCGGCCAATCCTGCCAATCCGGCCAGTCCGGTCTGTCAGGTCAGTCCGGCCT
GTCAGGCGTGCGGCGTTGTG

Product: diguanylate cyclase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MVNAAAVGRLLAVPLGLFVAAALAGLFMNTLPPSAGEVAPYAAWAALAGGMPLAERFRRSRAVFVLLLGCGVFLLLYNVL
PALGDATRVRLWYGIVLTLLPCNLLLFAFLEDRGVFSRWGMGMLAWLLGQFMTAAFIVRGGGVLLATSLGRTMQRAVEGW
VYASPLPGWFDSWTPLTQPALLLVLLAAVVLVVRVVRLEDGFSASLLGTLLSLTAAFHAVGNGPVVSLFVAAAGAAQLTA
LFRDSYSMAFVDELTEVPSRRALMADAKKLGGSYCVAMADVDHFKAFNDTYGHDVGDDVLRMVAARLAGVDGGKVYRYGG
EEFTILFPRLGRAEARPHLEAVREAVAAIPFRIRTRNKSAKVVRDKSVTVTVSIGLAQREDGEDDFESVLKKADKALYKA
KKAGRNKVALL

Sequences:

>Translated_411_residues
MVNAAAVGRLLAVPLGLFVAAALAGLFMNTLPPSAGEVAPYAAWAALAGGMPLAERFRRSRAVFVLLLGCGVFLLLYNVL
PALGDATRVRLWYGIVLTLLPCNLLLFAFLEDRGVFSRWGMGMLAWLLGQFMTAAFIVRGGGVLLATSLGRTMQRAVEGW
VYASPLPGWFDSWTPLTQPALLLVLLAAVVLVVRVVRLEDGFSASLLGTLLSLTAAFHAVGNGPVVSLFVAAAGAAQLTA
LFRDSYSMAFVDELTEVPSRRALMADAKKLGGSYCVAMADVDHFKAFNDTYGHDVGDDVLRMVAARLAGVDGGKVYRYGG
EEFTILFPRLGRAEARPHLEAVREAVAAIPFRIRTRNKSAKVVRDKSVTVTVSIGLAQREDGEDDFESVLKKADKALYKA
KKAGRNKVALL
>Mature_411_residues
MVNAAAVGRLLAVPLGLFVAAALAGLFMNTLPPSAGEVAPYAAWAALAGGMPLAERFRRSRAVFVLLLGCGVFLLLYNVL
PALGDATRVRLWYGIVLTLLPCNLLLFAFLEDRGVFSRWGMGMLAWLLGQFMTAAFIVRGGGVLLATSLGRTMQRAVEGW
VYASPLPGWFDSWTPLTQPALLLVLLAAVVLVVRVVRLEDGFSASLLGTLLSLTAAFHAVGNGPVVSLFVAAAGAAQLTA
LFRDSYSMAFVDELTEVPSRRALMADAKKLGGSYCVAMADVDHFKAFNDTYGHDVGDDVLRMVAARLAGVDGGKVYRYGG
EEFTILFPRLGRAEARPHLEAVREAVAAIPFRIRTRNKSAKVVRDKSVTVTVSIGLAQREDGEDDFESVLKKADKALYKA
KKAGRNKVALL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=262, Percent_Identity=29.3893129770992, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787262, Length=179, Percent_Identity=35.195530726257, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082007, Length=231, Percent_Identity=29.4372294372294, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787056, Length=188, Percent_Identity=33.5106382978723, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI145693134, Length=171, Percent_Identity=35.672514619883, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786584, Length=198, Percent_Identity=29.2929292929293, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081881, Length=141, Percent_Identity=36.8794326241135, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787816, Length=170, Percent_Identity=34.1176470588235, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787541, Length=175, Percent_Identity=30.2857142857143, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: NA

Molecular weight: Translated: 44107; Mature: 44107

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNAAAVGRLLAVPLGLFVAAALAGLFMNTLPPSAGEVAPYAAWAALAGGMPLAERFRRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
RAVFVLLLGCGVFLLLYNVLPALGDATRVRLWYGIVLTLLPCNLLLFAFLEDRGVFSRWG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MGMLAWLLGQFMTAAFIVRGGGVLLATSLGRTMQRAVEGWVYASPLPGWFDSWTPLTQPA
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCHHH
LLLVLLAAVVLVVRVVRLEDGFSASLLGTLLSLTAAFHAVGNGPVVSLFVAAAGAAQLTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
LFRDSYSMAFVDELTEVPSRRALMADAKKLGGSYCVAMADVDHFKAFNDTYGHDVGDDVL
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCCHHHHH
RMVAARLAGVDGGKVYRYGGEEFTILFPRLGRAEARPHLEAVREAVAAIPFRIRTRNKSA
HHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
KVVRDKSVTVTVSIGLAQREDGEDDFESVLKKADKALYKAKKAGRNKVALL
CEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MVNAAAVGRLLAVPLGLFVAAALAGLFMNTLPPSAGEVAPYAAWAALAGGMPLAERFRRS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHH
RAVFVLLLGCGVFLLLYNVLPALGDATRVRLWYGIVLTLLPCNLLLFAFLEDRGVFSRWG
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
MGMLAWLLGQFMTAAFIVRGGGVLLATSLGRTMQRAVEGWVYASPLPGWFDSWTPLTQPA
HHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCHHH
LLLVLLAAVVLVVRVVRLEDGFSASLLGTLLSLTAAFHAVGNGPVVSLFVAAAGAAQLTA
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHH
LFRDSYSMAFVDELTEVPSRRALMADAKKLGGSYCVAMADVDHFKAFNDTYGHDVGDDVL
HHHCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCCCHHHHH
RMVAARLAGVDGGKVYRYGGEEFTILFPRLGRAEARPHLEAVREAVAAIPFRIRTRNKSA
HHHHHHHCCCCCCCEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCC
KVVRDKSVTVTVSIGLAQREDGEDDFESVLKKADKALYKAKKAGRNKVALL
CEEECCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]