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The map label for this gene is tolB

Identifier: 78224723

GI number: 78224723

Start: 3973146

End: 3974435

Strand: Direct

Name: tolB

Synonym: Gmet_3540

Alternate gene names: 78224723

Gene position: 3973146-3974435 (Clockwise)

Preceding gene: 78224722

Following gene: 78224724

Centisome position: 99.39

GC content: 65.35

Gene sequence:

>1290_bases
ATGACACGACGTCTCTTCATTCTCATCACCATCATGCTCTGCCTGATCCCCGCACTCCTCCATTCCCAGGAAGGGTATCG
GGAAGTGACGGCTGCGGGAACCCACAAACTGACCCTCACCGTGGACTCCCCACGGCGCCTCGGCGGTTCCGATGCTCCCC
AGGTGGCCCGCGAGACGGCCGAAGCGCTCCAGTTCGACATGGTCCTTGCCGGCCCCTTCACCGTCACGGCCCCCTCGGCC
GCCGCGGCCTCGGCCGGCATCCGGCCGGGAGAGTTCGACTTCGCCCCCTGGCGCGGCGCCGGGGTCGACCTTCTGGTCAA
GAGCGGCTACACGGTTACGGGAAACGCCATGACCATGGAGTTCCGCCTCTACAACGTCACCCAGGGGAAGGAGATCCTGG
CCAAGCGGTACACGGGCCGTCCCGGCGATGTCCGGAGAATCGCCCATACCTTCTCGGACGACATCATGGCCGCCCTGACC
GGGGAGCGTGGCCCCTTCACGGGAAAAATCGCTTTTGTCTCCACCCGGGCCGGCGCCAAGGCGATCTTCCTCATGGACTA
TGACGGTCACAACGTCCAGCAGCTCACGAAAAACCGCTCCATCAACCTGAACCCCGATTTTTCCCCCAGCGGGAAGGAAA
TCATCTTCACCTCCTACCGCCAGGGAAATCCGGACCTCTTCCGCCGCGAGCTCTTCACCGGCGCCGAGGCCCGGATCTCC
TCCTACCGGGGGATCAACGCCACCGGCGCCTGGTCCCCCGACGGCAAGACCATCGCCCTGACCCTGAGCAAGGACGGCAA
CTCGGAGATCTACACCATCTCCCGGGACGGGAAGAACCCGCACCGGCTCACCAACACCAGCGCCATCGAGGTCTCCCCCG
CCTGGTCCCCCGACGTCCGCAAGATCGTCTTCGTCTCCGACCGGCTCGGCAAACCCCAGATTTTCATCATGAATGCCGAC
GGCACCGGGGTCCGGCGCCTCACCACCAGCGGCAACTACAACGTGAGCCCCCGCTGGTCCCCCAAGGGTGACCGGATCGT
CTACAGCCGCCAGGAGGGAGGGTTCCAGATCTACGCCATCAACCCCGACGGAACCAACGATACCAGGCTCACCAGCGAGG
GAAGCAACGAGCATCCCCGGTGGTCTCCCGACGGCCGTTTCATCACCTTCAGCTCCACCCGCGGCGGCGGTGAAGGGATT
TACGTGATGAGGGCCGACGGCAGCGGCCAGACCCGGGTTTCCGGCGGGAAGGGGAGGGACTCCCATCCCACGTGGTCGCC
GCGTTGGTGA

Upstream 100 bases:

>100_bases
ACAAGGCCATCGCCCGGGCCGAAAAGACCTTTGTCCCGCCCCCCGGCGGCGGCGAATTCGACTACGGTTTCATCTTCAGA
CCACAGGGAGTGGGAAAAAA

Downstream 100 bases:

>100_bases
GGCAGGTGTTGAAAATGAAGCACCGTTCTCTATAATGAAAACAGAATTTTCGGTGACCGGCCGGTGATCATGTTCCGGCA
GGCGATTGTCTAACAACAAA

Product: WD-40-like beta propeller repeat-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 428

Protein sequence:

>429_residues
MTRRLFILITIMLCLIPALLHSQEGYREVTAAGTHKLTLTVDSPRRLGGSDAPQVARETAEALQFDMVLAGPFTVTAPSA
AAASAGIRPGEFDFAPWRGAGVDLLVKSGYTVTGNAMTMEFRLYNVTQGKEILAKRYTGRPGDVRRIAHTFSDDIMAALT
GERGPFTGKIAFVSTRAGAKAIFLMDYDGHNVQQLTKNRSINLNPDFSPSGKEIIFTSYRQGNPDLFRRELFTGAEARIS
SYRGINATGAWSPDGKTIALTLSKDGNSEIYTISRDGKNPHRLTNTSAIEVSPAWSPDVRKIVFVSDRLGKPQIFIMNAD
GTGVRRLTTSGNYNVSPRWSPKGDRIVYSRQEGGFQIYAINPDGTNDTRLTSEGSNEHPRWSPDGRFITFSSTRGGGEGI
YVMRADGSGQTRVSGGKGRDSHPTWSPRW

Sequences:

>Translated_429_residues
MTRRLFILITIMLCLIPALLHSQEGYREVTAAGTHKLTLTVDSPRRLGGSDAPQVARETAEALQFDMVLAGPFTVTAPSA
AAASAGIRPGEFDFAPWRGAGVDLLVKSGYTVTGNAMTMEFRLYNVTQGKEILAKRYTGRPGDVRRIAHTFSDDIMAALT
GERGPFTGKIAFVSTRAGAKAIFLMDYDGHNVQQLTKNRSINLNPDFSPSGKEIIFTSYRQGNPDLFRRELFTGAEARIS
SYRGINATGAWSPDGKTIALTLSKDGNSEIYTISRDGKNPHRLTNTSAIEVSPAWSPDVRKIVFVSDRLGKPQIFIMNAD
GTGVRRLTTSGNYNVSPRWSPKGDRIVYSRQEGGFQIYAINPDGTNDTRLTSEGSNEHPRWSPDGRFITFSSTRGGGEGI
YVMRADGSGQTRVSGGKGRDSHPTWSPRW
>Mature_428_residues
TRRLFILITIMLCLIPALLHSQEGYREVTAAGTHKLTLTVDSPRRLGGSDAPQVARETAEALQFDMVLAGPFTVTAPSAA
AASAGIRPGEFDFAPWRGAGVDLLVKSGYTVTGNAMTMEFRLYNVTQGKEILAKRYTGRPGDVRRIAHTFSDDIMAALTG
ERGPFTGKIAFVSTRAGAKAIFLMDYDGHNVQQLTKNRSINLNPDFSPSGKEIIFTSYRQGNPDLFRRELFTGAEARISS
YRGINATGAWSPDGKTIALTLSKDGNSEIYTISRDGKNPHRLTNTSAIEVSPAWSPDVRKIVFVSDRLGKPQIFIMNADG
TGVRRLTTSGNYNVSPRWSPKGDRIVYSRQEGGFQIYAINPDGTNDTRLTSEGSNEHPRWSPDGRFITFSSTRGGGEGIY
VMRADGSGQTRVSGGKGRDSHPTWSPRW

Specific function: Involved in the tonB-independent uptake of proteins

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family

Homologues:

Organism=Escherichia coli, GI1786961, Length=346, Percent_Identity=28.0346820809249, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32564215, Length=211, Percent_Identity=27.4881516587678, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI32564217, Length=211, Percent_Identity=27.4881516587678, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TOLB_GEOMG (Q39PS9)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_386470.1
- ProteinModelPortal:   Q39PS9
- SMR:   Q39PS9
- STRING:   Q39PS9
- GeneID:   3739799
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_3540
- NMPDR:   fig|269799.3.peg.3508
- eggNOG:   COG0823
- HOGENOM:   HBG644919
- OMA:   RVADYDG
- PhylomeDB:   Q39PS9
- ProtClustDB:   CLSK827594
- BioCyc:   GMET269799:GMET_3540-MONOMER
- HAMAP:   MF_00671
- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943
- Gene3D:   G3DSA:3.40.50.10070
- Gene3D:   G3DSA:2.130.10.10
- TIGRFAMs:   TIGR02800

Pfam domain/function: PF07676 PD40; PF04052 TolB_N; SSF52964 TolB_N

EC number: NA

Molecular weight: Translated: 46908; Mature: 46777

Theoretical pI: Translated: 10.21; Mature: 10.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRLFILITIMLCLIPALLHSQEGYREVTAAGTHKLTLTVDSPRRLGGSDAPQVARETA
CCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCHHHCCCCCCHHHHHHHH
EALQFDMVLAGPFTVTAPSAAAASAGIRPGEFDFAPWRGAGVDLLVKSGYTVTGNAMTME
HHHEEEEEEECCEEEECCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECCEEEECCEEEEE
FRLYNVTQGKEILAKRYTGRPGDVRRIAHTFSDDIMAALTGERGPFTGKIAFVSTRAGAK
EEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCCCCEEEEEEEEECCCCE
AIFLMDYDGHNVQQLTKNRSINLNPDFSPSGKEIIFTSYRQGNPDLFRRELFTGAEARIS
EEEEEECCCCCHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHH
SYRGINATGAWSPDGKTIALTLSKDGNSEIYTISRDGKNPHRLTNTSAIEVSPAWSPDVR
HCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEECCCCEEEECCCCCCCCE
KIVFVSDRLGKPQIFIMNADGTGVRRLTTSGNYNVSPRWSPKGDRIVYSRQEGGFQIYAI
EEEEEECCCCCCEEEEEECCCCCEEEEEECCCEECCCCCCCCCCEEEEEECCCCEEEEEE
NPDGTNDTRLTSEGSNEHPRWSPDGRFITFSSTRGGGEGIYVMRADGSGQTRVSGGKGRD
CCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEECCCCCCC
SHPTWSPRW
CCCCCCCCC
>Mature Secondary Structure 
TRRLFILITIMLCLIPALLHSQEGYREVTAAGTHKLTLTVDSPRRLGGSDAPQVARETA
CCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEECCHHHCCCCCCHHHHHHHH
EALQFDMVLAGPFTVTAPSAAAASAGIRPGEFDFAPWRGAGVDLLVKSGYTVTGNAMTME
HHHEEEEEEECCEEEECCCHHHHHCCCCCCCCCCCCCCCCCEEEEEECCEEEECCEEEEE
FRLYNVTQGKEILAKRYTGRPGDVRRIAHTFSDDIMAALTGERGPFTGKIAFVSTRAGAK
EEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHEEEEECCCCCCCEEEEEEEEECCCCE
AIFLMDYDGHNVQQLTKNRSINLNPDFSPSGKEIIFTSYRQGNPDLFRRELFTGAEARIS
EEEEEECCCCCHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCHHHHH
SYRGINATGAWSPDGKTIALTLSKDGNSEIYTISRDGKNPHRLTNTSAIEVSPAWSPDVR
HCCCCCCCCCCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEECCCCEEEECCCCCCCCE
KIVFVSDRLGKPQIFIMNADGTGVRRLTTSGNYNVSPRWSPKGDRIVYSRQEGGFQIYAI
EEEEEECCCCCCEEEEEECCCCCEEEEEECCCEECCCCCCCCCCEEEEEECCCCEEEEEE
NPDGTNDTRLTSEGSNEHPRWSPDGRFITFSSTRGGGEGIYVMRADGSGQTRVSGGKGRD
CCCCCCCCEEECCCCCCCCCCCCCCCEEEEECCCCCCCEEEEEEECCCCCEEECCCCCCC
SHPTWSPRW
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA