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The map label for this gene is polC [H]

Identifier: 78224248

GI number: 78224248

Start: 3450169

End: 3450825

Strand: Reverse

Name: polC [H]

Synonym: Gmet_3055

Alternate gene names: 78224248

Gene position: 3450825-3450169 (Counterclockwise)

Preceding gene: 78224249

Following gene: 78224247

Centisome position: 86.33

GC content: 61.95

Gene sequence:

>657_bases
ATGGGGAAGTTTACCGCCGTCATCCTCGACTTCGAGACAACTGGCCTCTCACCTGGCTATGGCGACCGGGCCATCGAGAT
CGGTGCGGTACTGGTCCGGAACAACCGGATTGTTGACAGATTCCAGAGTCTCATGAATCCCGGCAAGAGGGTGAGCGGTT
TCATCGAGGAATACACCGGCATCACCAATGAGATGCTGGCCAAGGCCCCACCTGCGGAGCAGGTGATGGAGCGGTTTGCC
TCCTTCATTGCCGGCCACAACCTGGTTGCCCACAACGCGAGCTTCGACCGCCGCTTCCTGGACGCCGAACTCTCCCGCAT
CGGCAGGAGACGGACCGGGGACTTTGCCTGCTCAATGCTCGCTGCCCGCCGGATCTACACCAATGCCCCAAACCACCGCC
TGGAGACGCTGGTCAGCCACCTCAGCCTCCAGACAGACGGAGTCTTCCACCGGGCTCTCGCCGATGCGGAAATGACCGCC
CAGCTCTGGACGGCGATGATCGGGGAACTCAAGGAAACCTATCGCCTCAGGCAGGTCACCTTTGAGCTCATGCACTCCCT
CGGCGGCGTGGCGAAGAAGGCAGTGCCGGCCTATCTTGAACGGGTGGCGGCAGGGCGAACTTGTGCCTGTGCGAGGGGCG
GGCGATCTTTAGGCTGA

Upstream 100 bases:

>100_bases
GCAATCCCCAGACCGGCGAGGCGCTCACCATCCAGTCCCGGCGAGTGCTGACATTCAAGCCCAGTGCGGTCCTCAAGAAC
TCCATCAACGGGGAGTCCTG

Downstream 100 bases:

>100_bases
CCAGGGAGGGGTAGATGTCGACAGAAGAAAGAGACGGGGGATTATCGCAACGCGGGAGCAACAAACGGTTGCTGCTGTTG
GCTACTATACTTGTCCCGTT

Product: DNA polymerase III subunit epsilon

Products: NA

Alternate protein names: PolIII [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MGKFTAVILDFETTGLSPGYGDRAIEIGAVLVRNNRIVDRFQSLMNPGKRVSGFIEEYTGITNEMLAKAPPAEQVMERFA
SFIAGHNLVAHNASFDRRFLDAELSRIGRRRTGDFACSMLAARRIYTNAPNHRLETLVSHLSLQTDGVFHRALADAEMTA
QLWTAMIGELKETYRLRQVTFELMHSLGGVAKKAVPAYLERVAAGRTCACARGGRSLG

Sequences:

>Translated_218_residues
MGKFTAVILDFETTGLSPGYGDRAIEIGAVLVRNNRIVDRFQSLMNPGKRVSGFIEEYTGITNEMLAKAPPAEQVMERFA
SFIAGHNLVAHNASFDRRFLDAELSRIGRRRTGDFACSMLAARRIYTNAPNHRLETLVSHLSLQTDGVFHRALADAEMTA
QLWTAMIGELKETYRLRQVTFELMHSLGGVAKKAVPAYLERVAAGRTCACARGGRSLG
>Mature_217_residues
GKFTAVILDFETTGLSPGYGDRAIEIGAVLVRNNRIVDRFQSLMNPGKRVSGFIEEYTGITNEMLAKAPPAEQVMERFAS
FIAGHNLVAHNASFDRRFLDAELSRIGRRRTGDFACSMLAARRIYTNAPNHRLETLVSHLSLQTDGVFHRALADAEMTAQ
LWTAMIGELKETYRLRQVTFELMHSLGGVAKKAVPAYLERVAAGRTCACARGGRSLG

Specific function: Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG2176

COG function: function code L; DNA polymerase III, alpha subunit (gram-positive type)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1786409, Length=174, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR006054
- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR006308
- InterPro:   IPR012337 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF00929 Exonuc_X-T; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 24064; Mature: 23933

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKFTAVILDFETTGLSPGYGDRAIEIGAVLVRNNRIVDRFQSLMNPGKRVSGFIEEYTG
CCCEEEEEEEEECCCCCCCCCCCHHHHHHHHEECCHHHHHHHHHHCCCHHHHHHHHHHHC
ITNEMLAKAPPAEQVMERFASFIAGHNLVAHNASFDRRFLDAELSRIGRRRTGDFACSML
CHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
AARRIYTNAPNHRLETLVSHLSLQTDGVFHRALADAEMTAQLWTAMIGELKETYRLRQVT
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FELMHSLGGVAKKAVPAYLERVAAGRTCACARGGRSLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC
>Mature Secondary Structure 
GKFTAVILDFETTGLSPGYGDRAIEIGAVLVRNNRIVDRFQSLMNPGKRVSGFIEEYTG
CCEEEEEEEEECCCCCCCCCCCHHHHHHHHEECCHHHHHHHHHHCCCHHHHHHHHHHHC
ITNEMLAKAPPAEQVMERFASFIAGHNLVAHNASFDRRFLDAELSRIGRRRTGDFACSML
CHHHHHHCCCCHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHH
AARRIYTNAPNHRLETLVSHLSLQTDGVFHRALADAEMTAQLWTAMIGELKETYRLRQVT
HHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FELMHSLGGVAKKAVPAYLERVAAGRTCACARGGRSLG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721630 [H]