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The map label for this gene is miaB

Identifier: 78223499

GI number: 78223499

Start: 2602571

End: 2603896

Strand: Reverse

Name: miaB

Synonym: Gmet_2296

Alternate gene names: 78223499

Gene position: 2603896-2602571 (Counterclockwise)

Preceding gene: 78223501

Following gene: 78223498

Centisome position: 65.14

GC content: 62.37

Gene sequence:

>1326_bases
GTGACCGGCGAAAAACTTCTGTATATCGAAACCTTTGGTTGCCAGATGAACGTGAGCGATTCCGAGAAGGTGGCGTCGCT
CCTGAGGGGGGAGGGGTACTCCCAGACTCCCGATTCATCGGAGGCGGACCTGATTATTGTGAACACCTGCAGCGTGCGGG
CAAAGGCCGAGCACAAGGTCTACAGCTACCTGGGCCGCTTTCGGAAGCTTAAGCGTGACAGACGGTTGCTTCTGGGCGTG
GGGGGGTGCGTGGCGCAGCAGGAGGGGGAACGGCTCCTGAAGCGGGTGCCGTGGCTCGATCTCGTCTTCGGAACGCACAA
CCTCCATCTTTTGCCGGAGATGGTCAGGGCCGCGGAGCAGGGAGAGCGCAGGGCCGCGGTGGATTTCATCGACAACGAGG
CAAGGCTCGACTTGTTCCCGCAAGCCGACGAAGGGGGGGGCGTAACCCGCTTCGTGACGGTCATGCAGGGGTGCGACAAC
TTCTGCTCCTACTGCATCGTCCCTTACGTGCGGGGAAGAGAGATCAGCCGCCGTTCCGTCGAGATCATCGGAGAGATTCG
CAGCGCCGTGGCTGGTGGCGTGCGGGAGGTGACGCTTCTGGGCCAGAATGTCAACTCCTACGGGCTGAAGACGCCGGGTG
AGCTGAGTTTTGCGGGGCTCCTGCGGGAAATTTCGGCCATCGACGGCCTGGAGCGGATCCGCTTCACCACCTCCCACCCG
AAGGATATCTCACCGGAGCTCATTGCCTGCTTCGCCGAGCTTCCCAAGCTCTGCGGACACATCCATCTGCCGGCCCAGGC
GGGGAGCGATTCGATTCTGGCACGCATGAACAGGGGCTATACGCGGCAGGAATACCTGGAAAAGGTGGCAGCGCTCAGGG
CGGCTCGCCCGGAGATCCTCATAACCGGGGACATCATCGTCGGCTTCCCCGGTGAGACGGAGGCCGATTTTCTCCAGACC
CTCTCCCTCATGGAGGAGGTCCGCTATACCGACATCTTTTCCTTTGCCTATTCCCCCCGCCCCGAGACCGCCGCCGCGTC
CCTCGGCGACAGGATTATGCGCAAGGAGACCACGGAGCGCCTGGAACGGGTTCAGGGGCTCCAGCGGGACATGACCATTG
AGAGGCATGCCGGCTTTGTCGGCACCTGCCAGGCGGTCCTCGTGGAAGGGATGAGCAAGCGGGGAGACCAGCTCTACGGC
CGCACCGACGGCAACCTGATCGTGAACTTTGCCGGCAATCCCTCCCTCGCAGGTTCCCTCGTCGACGTGCGCATCACTCG
CGGCTACCCGAATTCGCTCCTGGGCGAACTCGCAGTTTTTCAATAA

Upstream 100 bases:

>100_bases
GGACTTTATCCCGCTTGTGCGGTATACGGCAAATACGCAGTTGACGGGACGCGGCGTAATTTCGTAATATCTATCGAATT
ATCCGCAGGAAAGAGGACCC

Downstream 100 bases:

>100_bases
GAAAACGGAGCACCCATGTACCTGAAGATGAACATCCACGGCTTTGCCCTCGACTCCATCGCCCAGAGGCCGGTCGTCCT
CCTCAAGGATGCCGATGAGG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MTGEKLLYIETFGCQMNVSDSEKVASLLRGEGYSQTPDSSEADLIIVNTCSVRAKAEHKVYSYLGRFRKLKRDRRLLLGV
GGCVAQQEGERLLKRVPWLDLVFGTHNLHLLPEMVRAAEQGERRAAVDFIDNEARLDLFPQADEGGGVTRFVTVMQGCDN
FCSYCIVPYVRGREISRRSVEIIGEIRSAVAGGVREVTLLGQNVNSYGLKTPGELSFAGLLREISAIDGLERIRFTTSHP
KDISPELIACFAELPKLCGHIHLPAQAGSDSILARMNRGYTRQEYLEKVAALRAARPEILITGDIIVGFPGETEADFLQT
LSLMEEVRYTDIFSFAYSPRPETAAASLGDRIMRKETTERLERVQGLQRDMTIERHAGFVGTCQAVLVEGMSKRGDQLYG
RTDGNLIVNFAGNPSLAGSLVDVRITRGYPNSLLGELAVFQ

Sequences:

>Translated_441_residues
MTGEKLLYIETFGCQMNVSDSEKVASLLRGEGYSQTPDSSEADLIIVNTCSVRAKAEHKVYSYLGRFRKLKRDRRLLLGV
GGCVAQQEGERLLKRVPWLDLVFGTHNLHLLPEMVRAAEQGERRAAVDFIDNEARLDLFPQADEGGGVTRFVTVMQGCDN
FCSYCIVPYVRGREISRRSVEIIGEIRSAVAGGVREVTLLGQNVNSYGLKTPGELSFAGLLREISAIDGLERIRFTTSHP
KDISPELIACFAELPKLCGHIHLPAQAGSDSILARMNRGYTRQEYLEKVAALRAARPEILITGDIIVGFPGETEADFLQT
LSLMEEVRYTDIFSFAYSPRPETAAASLGDRIMRKETTERLERVQGLQRDMTIERHAGFVGTCQAVLVEGMSKRGDQLYG
RTDGNLIVNFAGNPSLAGSLVDVRITRGYPNSLLGELAVFQ
>Mature_440_residues
TGEKLLYIETFGCQMNVSDSEKVASLLRGEGYSQTPDSSEADLIIVNTCSVRAKAEHKVYSYLGRFRKLKRDRRLLLGVG
GCVAQQEGERLLKRVPWLDLVFGTHNLHLLPEMVRAAEQGERRAAVDFIDNEARLDLFPQADEGGGVTRFVTVMQGCDNF
CSYCIVPYVRGREISRRSVEIIGEIRSAVAGGVREVTLLGQNVNSYGLKTPGELSFAGLLREISAIDGLERIRFTTSHPK
DISPELIACFAELPKLCGHIHLPAQAGSDSILARMNRGYTRQEYLEKVAALRAARPEILITGDIIVGFPGETEADFLQTL
SLMEEVRYTDIFSFAYSPRPETAAASLGDRIMRKETTERLERVQGLQRDMTIERHAGFVGTCQAVLVEGMSKRGDQLYGR
TDGNLIVNFAGNPSLAGSLVDVRITRGYPNSLLGELAVFQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=39.0756302521008, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=39.0756302521008, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI93277076, Length=440, Percent_Identity=28.1818181818182, Blast_Score=152, Evalue=9e-37,
Organism=Escherichia coli, GI1786882, Length=439, Percent_Identity=46.0136674259681, Blast_Score=380, Evalue=1e-106,
Organism=Escherichia coli, GI1787057, Length=395, Percent_Identity=26.8354430379747, Blast_Score=154, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=467, Percent_Identity=35.7601713062098, Blast_Score=265, Evalue=3e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=376, Percent_Identity=27.3936170212766, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.1824480369515, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI19922432, Length=450, Percent_Identity=28, Blast_Score=156, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_GEOMG (Q39TA3)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_385246.1
- ProteinModelPortal:   Q39TA3
- SMR:   Q39TA3
- STRING:   Q39TA3
- GeneID:   3739324
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_2296
- NMPDR:   fig|269799.3.peg.2540
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   EVRTYGC
- PhylomeDB:   Q39TA3
- ProtClustDB:   PRK14325
- BioCyc:   GMET269799:GMET_2296-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 48771; Mature: 48640

Theoretical pI: Translated: 6.41; Mature: 6.41

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGEKLLYIETFGCQMNVSDSEKVASLLRGEGYSQTPDSSEADLIIVNTCSVRAKAEHKV
CCCCEEEEEEEECCEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
YSYLGRFRKLKRDRRLLLGVGGCVAQQEGERLLKRVPWLDLVFGTHNLHLLPEMVRAAEQ
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHC
GERRAAVDFIDNEARLDLFPQADEGGGVTRFVTVMQGCDNFCSYCIVPYVRGREISRRSV
CCCHHHHHEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EIIGEIRSAVAGGVREVTLLGQNVNSYGLKTPGELSFAGLLREISAIDGLERIRFTTSHP
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHEEECCCC
KDISPELIACFAELPKLCGHIHLPAQAGSDSILARMNRGYTRQEYLEKVAALRAARPEIL
CCCCHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEE
ITGDIIVGFPGETEADFLQTLSLMEEVRYTDIFSFAYSPRPETAAASLGDRIMRKETTER
EECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LERVQGLQRDMTIERHAGFVGTCQAVLVEGMSKRGDQLYGRTDGNLIVNFAGNPSLAGSL
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCCE
VDVRITRGYPNSLLGELAVFQ
EEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
TGEKLLYIETFGCQMNVSDSEKVASLLRGEGYSQTPDSSEADLIIVNTCSVRAKAEHKV
CCCEEEEEEEECCEECCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHH
YSYLGRFRKLKRDRRLLLGVGGCVAQQEGERLLKRVPWLDLVFGTHNLHLLPEMVRAAEQ
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHC
GERRAAVDFIDNEARLDLFPQADEGGGVTRFVTVMQGCDNFCSYCIVPYVRGREISRRSV
CCCHHHHHEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
EIIGEIRSAVAGGVREVTLLGQNVNSYGLKTPGELSFAGLLREISAIDGLERIRFTTSHP
HHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHEEECCCC
KDISPELIACFAELPKLCGHIHLPAQAGSDSILARMNRGYTRQEYLEKVAALRAARPEIL
CCCCHHHHHHHHHHHHHHCEEECCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEE
ITGDIIVGFPGETEADFLQTLSLMEEVRYTDIFSFAYSPRPETAAASLGDRIMRKETTER
EECCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHH
LERVQGLQRDMTIERHAGFVGTCQAVLVEGMSKRGDQLYGRTDGNLIVNFAGNPSLAGSL
HHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEECCCCCCCCCE
VDVRITRGYPNSLLGELAVFQ
EEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA