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The map label for this gene is amiC [H]

Identifier: 78222637

GI number: 78222637

Start: 1593396

End: 1594667

Strand: Direct

Name: amiC [H]

Synonym: Gmet_1425

Alternate gene names: 78222637

Gene position: 1593396-1594667 (Clockwise)

Preceding gene: 78222636

Following gene: 78222638

Centisome position: 39.86

GC content: 57.7

Gene sequence:

>1272_bases
GTGAGAATACAACGATGGTTACGGATAGTGCCCCTTCTCTTCGCCCTTGCAGGAGTGCTGTTTCTTTTTTCTTCTGCCGA
GGCCAAGGAGGTGCGCCGCGTTAAGGGCTCCAGCGTTAAGGCAACGGCCGTGGTGAAGGAGATGCGTCATTGGTCGAACC
CCGATTATACCCGGATTGCCATCACCGTCGACCGAGAAGCACATTTTGAAACCCACCAGATCAAGCAGCATCCGGGCGAT
GCTCTTCCGTCACGCATTTACCTCGATATAACCGGCGCCAGGGTTGGACCCGGCGTACACGACCTTCAAATAGCCGATCA
GCAGCTGAAATCGGCAAGGGTCGGCCAGTACAAGCCGGATGTGGTGCGGGTGGTTCTCGATGTTGACCGGATCAAGGAAT
TCAAGGTGTTTTCCCTCTCGGACCCGTTCCGGATCATCGTCGATGTGAAGGGAGAACGTCCCCTGGAATTCGCGAAACTG
ACGGAGCAGGTCCAGCCGGCACCGGCGCCGACAAGTGCTGCCGAGACGAAAGCCGCCGATACCAGTGATGAAAAACCGAG
CCCCAAGCCAGTAGCCAAATTCAAGCCGGGAAAGATTCGGCGGATTGTGGTCGATCCGGGTCACGGCGGTCACGACCCCG
GAGCGATTGGCGCGGGGGGGACCCAGGAAAAGGACGTTGTGCTTGCCCTCGGGCTCCAACTGGCGGCGAAGATTCGGGAA
GAGTTGGGCATTGACGTGGTCATGACCCGTTCAACCGATGTCTTCATCCCGCTGGAAGAGCGTACCGCCATCGCCAACAA
GGTGAATGCCGATCTCTTCGTGTCGGTCCATGCCAATGCATCCCTCAACCGCGGTACTTCGGGGATAGAGACCTATTACC
TCAATCTGGCCAAGACCGAGAAAGCTGCCCAGCTTGCTGCCCGGGAAAACGGCACATCCCTGGACAAGGTGAGCCTTCTG
CAGGCGATTCTCTTCGACCTGATGGCCAACTACAAGCTCAACGATTCCGCCCACCTGGCCGATGAAGTCCAGAAGGCCCT
CTACAAAAAGATCAACGGCCACTATCCGGGAACACGGAACCTGGGGGTCAAGCAGGGGCCATTTTACGTTCTTGTGGGTG
CCACCATGCCGAGCATCCTCGTGGAGACGGCTTTCATCAGCAACGAAACCGAGGAGGCCCGGCTTCGGGACAAGGCCTTT
CTTGATCGGGCTGCCGACGGCATTGTGGATGGAATTAAGGGGTATATCCGTACAATCGGCACCGGGCGATAG

Upstream 100 bases:

>100_bases
GACGATCTTCTCCGGCAGCGGCTGACCGGGTTGAATATCGCGGCACTCACTCCCCTTGAGGCGCTCAATATTCTCGATGA
GTTAAAGAGGATGGTCTGAC

Downstream 100 bases:

>100_bases
GTAAGGGATTTTATGGAATTCAACATTGACCGCTACTTTCCCGACACCTCTCAGGAGACTGGTGATGCCGGCCGTGCCTC
CTTCGAGGAGAAGCGTCCCC

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MRIQRWLRIVPLLFALAGVLFLFSSAEAKEVRRVKGSSVKATAVVKEMRHWSNPDYTRIAITVDREAHFETHQIKQHPGD
ALPSRIYLDITGARVGPGVHDLQIADQQLKSARVGQYKPDVVRVVLDVDRIKEFKVFSLSDPFRIIVDVKGERPLEFAKL
TEQVQPAPAPTSAAETKAADTSDEKPSPKPVAKFKPGKIRRIVVDPGHGGHDPGAIGAGGTQEKDVVLALGLQLAAKIRE
ELGIDVVMTRSTDVFIPLEERTAIANKVNADLFVSVHANASLNRGTSGIETYYLNLAKTEKAAQLAARENGTSLDKVSLL
QAILFDLMANYKLNDSAHLADEVQKALYKKINGHYPGTRNLGVKQGPFYVLVGATMPSILVETAFISNETEEARLRDKAF
LDRAADGIVDGIKGYIRTIGTGR

Sequences:

>Translated_423_residues
MRIQRWLRIVPLLFALAGVLFLFSSAEAKEVRRVKGSSVKATAVVKEMRHWSNPDYTRIAITVDREAHFETHQIKQHPGD
ALPSRIYLDITGARVGPGVHDLQIADQQLKSARVGQYKPDVVRVVLDVDRIKEFKVFSLSDPFRIIVDVKGERPLEFAKL
TEQVQPAPAPTSAAETKAADTSDEKPSPKPVAKFKPGKIRRIVVDPGHGGHDPGAIGAGGTQEKDVVLALGLQLAAKIRE
ELGIDVVMTRSTDVFIPLEERTAIANKVNADLFVSVHANASLNRGTSGIETYYLNLAKTEKAAQLAARENGTSLDKVSLL
QAILFDLMANYKLNDSAHLADEVQKALYKKINGHYPGTRNLGVKQGPFYVLVGATMPSILVETAFISNETEEARLRDKAF
LDRAADGIVDGIKGYIRTIGTGR
>Mature_423_residues
MRIQRWLRIVPLLFALAGVLFLFSSAEAKEVRRVKGSSVKATAVVKEMRHWSNPDYTRIAITVDREAHFETHQIKQHPGD
ALPSRIYLDITGARVGPGVHDLQIADQQLKSARVGQYKPDVVRVVLDVDRIKEFKVFSLSDPFRIIVDVKGERPLEFAKL
TEQVQPAPAPTSAAETKAADTSDEKPSPKPVAKFKPGKIRRIVVDPGHGGHDPGAIGAGGTQEKDVVLALGLQLAAKIRE
ELGIDVVMTRSTDVFIPLEERTAIANKVNADLFVSVHANASLNRGTSGIETYYLNLAKTEKAAQLAARENGTSLDKVSLL
QAILFDLMANYKLNDSAHLADEVQKALYKKINGHYPGTRNLGVKQGPFYVLVGATMPSILVETAFISNETEEARLRDKAF
LDRAADGIVDGIKGYIRTIGTGR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=424, Percent_Identity=31.3679245283019, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788776, Length=237, Percent_Identity=40.5063291139241, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1790611, Length=265, Percent_Identity=32.0754716981132, Blast_Score=112, Evalue=6e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46328; Mature: 46328

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIQRWLRIVPLLFALAGVLFLFSSAEAKEVRRVKGSSVKATAVVKEMRHWSNPDYTRIA
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEE
ITVDREAHFETHQIKQHPGDALPSRIYLDITGARVGPGVHDLQIADQQLKSARVGQYKPD
EEECCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
VVRVVLDVDRIKEFKVFSLSDPFRIIVDVKGERPLEFAKLTEQVQPAPAPTSAAETKAAD
HHEEEHHHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCC
TSDEKPSPKPVAKFKPGKIRRIVVDPGHGGHDPGAIGAGGTQEKDVVLALGLQLAAKIRE
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHH
ELGIDVVMTRSTDVFIPLEERTAIANKVNADLFVSVHANASLNRGTSGIETYYLNLAKTE
HCCCEEEEECCCEEEEECHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEHHHHH
KAAQLAARENGTSLDKVSLLQAILFDLMANYKLNDSAHLADEVQKALYKKINGHYPGTRN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LGVKQGPFYVLVGATMPSILVETAFISNETEEARLRDKAFLDRAADGIVDGIKGYIRTIG
CCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGR
CCC
>Mature Secondary Structure
MRIQRWLRIVPLLFALAGVLFLFSSAEAKEVRRVKGSSVKATAVVKEMRHWSNPDYTRIA
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEE
ITVDREAHFETHQIKQHPGDALPSRIYLDITGARVGPGVHDLQIADQQLKSARVGQYKPD
EEECCCCCCCHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCC
VVRVVLDVDRIKEFKVFSLSDPFRIIVDVKGERPLEFAKLTEQVQPAPAPTSAAETKAAD
HHEEEHHHHHHCCEEEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHCCCCC
TSDEKPSPKPVAKFKPGKIRRIVVDPGHGGHDPGAIGAGGTQEKDVVLALGLQLAAKIRE
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHH
ELGIDVVMTRSTDVFIPLEERTAIANKVNADLFVSVHANASLNRGTSGIETYYLNLAKTE
HCCCEEEEECCCEEEEECHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCEEEEEEHHHHH
KAAQLAARENGTSLDKVSLLQAILFDLMANYKLNDSAHLADEVQKALYKKINGHYPGTRN
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LGVKQGPFYVLVGATMPSILVETAFISNETEEARLRDKAFLDRAADGIVDGIKGYIRTIG
CCCCCCCEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TGR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]