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The map label for this gene is pcm

Identifier: 78222632

GI number: 78222632

Start: 1587966

End: 1588616

Strand: Direct

Name: pcm

Synonym: Gmet_1420

Alternate gene names: 78222632

Gene position: 1587966-1588616 (Clockwise)

Preceding gene: 78222631

Following gene: 78222633

Centisome position: 39.72

GC content: 52.23

Gene sequence:

>651_bases
ATGAATTTTGACATCGCACGAAAACGGATGGTTGAAACGCAAATTATCTCTCGCGGCGTGAAAGACCGGCGCCTTATAGA
GGCGATGCTAAAGGTCCCCCGTCACGTTTTTGTCGAAGAGGCGATGGCAGCCCAAGCTTACAGCGACACCCCGCTTCCAA
TCGGGGAGAAGCAAACCATCTCGCAGCCTTACATGGTTGCTCTCATGACCGAATTGCTTGAGCTTTCGGGCCGGGAGAAG
GTGCTAGAGATCGGCACTGGCTCAGGGTATCAAGCGGCAATACTTGCAACCCTCGCAGATCGCGTTTACACCGTTGAGCG
GATCCGGCCCTTGGCACTCAAGGCTCGTAGGGCACTTGACCGTCTCGGCCTCCTGAATGTGAACATAAAAATTTCTGATG
GGACCATCGGTTGGGAGGAAGAAGCCCCCTTTGACGCCATCATCGTTACGGCTGGAGCGCCGGATGTCCCGGATAAACTG
GCTGAACAGCTTGCTGTTGGTGGAAGGCTTGTTATTCCTGTAGGGAATCAGTTCGATCAGGTTCTTGTCAGGATTACCAA
GCAGGAAGATGGTTCTCTCATCAGAGAAAATGTAACAGGATGCAGATTCGTTAAACTGGTGGGCAAATACGGATGGGGTA
CTGAAGAGTAA

Upstream 100 bases:

>100_bases
TGTCAACCGACGTTGCGCCCGGATGAGCCCTTGTCCTAACGCTGGACAGGACTGAGGTGGTCTGTTATAGTCTGCATTTC
AGCTAAAACAGGGGATTTGG

Downstream 100 bases:

>100_bases
GCGGCTAAATCTGCTTTGCTGAACGGGGAAGAACATGGATGATTTTCTCGAAAAGGAAGCCGTTGAACCTGAAGAACATG
CCGAGCGGCAACCTGACGAT

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MNFDIARKRMVETQIISRGVKDRRLIEAMLKVPRHVFVEEAMAAQAYSDTPLPIGEKQTISQPYMVALMTELLELSGREK
VLEIGTGSGYQAAILATLADRVYTVERIRPLALKARRALDRLGLLNVNIKISDGTIGWEEEAPFDAIIVTAGAPDVPDKL
AEQLAVGGRLVIPVGNQFDQVLVRITKQEDGSLIRENVTGCRFVKLVGKYGWGTEE

Sequences:

>Translated_216_residues
MNFDIARKRMVETQIISRGVKDRRLIEAMLKVPRHVFVEEAMAAQAYSDTPLPIGEKQTISQPYMVALMTELLELSGREK
VLEIGTGSGYQAAILATLADRVYTVERIRPLALKARRALDRLGLLNVNIKISDGTIGWEEEAPFDAIIVTAGAPDVPDKL
AEQLAVGGRLVIPVGNQFDQVLVRITKQEDGSLIRENVTGCRFVKLVGKYGWGTEE
>Mature_216_residues
MNFDIARKRMVETQIISRGVKDRRLIEAMLKVPRHVFVEEAMAAQAYSDTPLPIGEKQTISQPYMVALMTELLELSGREK
VLEIGTGSGYQAAILATLADRVYTVERIRPLALKARRALDRLGLLNVNIKISDGTIGWEEEAPFDAIIVTAGAPDVPDKL
AEQLAVGGRLVIPVGNQFDQVLVRITKQEDGSLIRENVTGCRFVKLVGKYGWGTEE

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=201, Percent_Identity=35.3233830845771, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1789100, Length=194, Percent_Identity=51.5463917525773, Blast_Score=185, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI71983477, Length=183, Percent_Identity=35.5191256830601, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI193207222, Length=181, Percent_Identity=34.2541436464088, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17981723, Length=177, Percent_Identity=35.0282485875706, Blast_Score=84, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_GEOMG (Q39VS0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384379.1
- ProteinModelPortal:   Q39VS0
- SMR:   Q39VS0
- STRING:   Q39VS0
- GeneID:   3738549
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1420
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   MGAKEDL
- PhylomeDB:   Q39VS0
- ProtClustDB:   CLSK828403
- BioCyc:   GMET269799:GMET_1420-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23890; Mature: 23890

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 61-61

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFDIARKRMVETQIISRGVKDRRLIEAMLKVPRHVFVEEAMAAQAYSDTPLPIGEKQTI
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SQPYMVALMTELLELSGREKVLEIGTGSGYQAAILATLADRVYTVERIRPLALKARRALD
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGLLNVNIKISDGTIGWEEEAPFDAIIVTAGAPDVPDKLAEQLAVGGRLVIPVGNQFDQ
HCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCCHHHH
VLVRITKQEDGSLIRENVTGCRFVKLVGKYGWGTEE
HHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNFDIARKRMVETQIISRGVKDRRLIEAMLKVPRHVFVEEAMAAQAYSDTPLPIGEKQTI
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SQPYMVALMTELLELSGREKVLEIGTGSGYQAAILATLADRVYTVERIRPLALKARRALD
CCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLGLLNVNIKISDGTIGWEEEAPFDAIIVTAGAPDVPDKLAEQLAVGGRLVIPVGNQFDQ
HCCEEEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCEEEEECCCHHHH
VLVRITKQEDGSLIRENVTGCRFVKLVGKYGWGTEE
HHHHHHCCCCCHHHHHCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA