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The map label for this gene is rpoN [H]

Identifier: 78222497

GI number: 78222497

Start: 1443385

End: 1444836

Strand: Direct

Name: rpoN [H]

Synonym: Gmet_1283

Alternate gene names: 78222497

Gene position: 1443385-1444836 (Clockwise)

Preceding gene: 78222496

Following gene: 78222498

Centisome position: 36.11

GC content: 56.13

Gene sequence:

>1452_bases
ATGGCCATTGAAATGCGTCAACAGATGAAGCTGACCCAGCAACTGGTGATGACTCCCCAGTTGCAGCAGGCCATCAAGCT
TCTCCAGCTCTCACGGCTTGAGCTTCAGGATCTTGTCCGTCAGGAAATGGAGGAGAATCCGGTCCTTGAGGAAACCCTCG
AACTGGAGGAACCCAAAGAGCAGGACCAGTTGGAGCAGTCCGAAAAGGAAGAGACACCTTCTGGTGAGGACAACAAGGAG
TTCCAGGAGGTGAAAGCGGGCGAGGAAACGCTGCGCGAAACCGACTGGGACAGCTATCTCGAAGGTTATAATTTCAGCGC
GGGCGAGCAGTATTACGACGACGAAGATCGCCCCTCCTACGAAAATGTCCTGACCCGCAAGGGCACGCTCGGTGATCACC
TCATGTGGCAGTTGAGCCTCACCCGGTTTTCTGACGTGGAGACGGCGGTGGGTGCCGAGATTATAGGCAATATCGATGAA
GACGGCTATTTCCGTGCCACAGTGGAGGAAATTGCGCGATCGCAGGGCGTTGACGAAGCCCTTGTCGAGACATGTCTCAA
AAAGGTTCAGGAATTCGACCCCATGGGGGTTGGTGCGCGGGACCTCAGGGAGTGCCTCCTTATTCAGGTGGAACAGCTTG
GAATGGGGGGAAGCGTCGTGGAGGGGATCCTGCGTCATCACCTCCACGATCTTGAAGCCCGCAAATACAAGCAGATTGCC
AAGGCCCTCGGGGTCGACATCAACGATGTCCTCATGGGGGCGCGGATCATTGCCGGGTTTGATCCCAAGCCGGGGCGGGT
CTATGGCTCCGAGGACGTCCACTATATTTCCGCCGACATTTTCGTTTACAAGATCGGAGAGGATTACGTGGTGGTGCTGA
ACGACGAGGGACTCCCCAACCTCCGGATCAGCCCCTACTACGCGGGAGAAGCCAAGGCGAGCGGCCAGGTTGATGCCAAG
GCCGAGGAATACATCAACGACAAGGTCCGTTCGGCCATGTGGCTCATTAAGAGTATTCACCAGCGGCAGCGGACCATTTA
CAAAGTGGCCAAGAGCATCTCCAAGTTCCAGCGCGATTTTCTCGACAAGGGGATTGAATATCTTCGCCCCCTGGTGCTCC
GCGATGTGGCCGAGGATATCGGCATGCACGAGTCGACCATCAGCCGCGTCACCACCAACAAGTACATGCAGACCCCCCAG
GGGCTCTTCGAAATGAAGTATTTCTTCAACAGCGGCATTTCCACGGGAGAGGGTGATTTCATCGCCTCCGAGAGTGTCAA
GAACAAGATCAAGGAAATCGTCGAGGCCGAGGATCCCCGTAAGCCTTACAGCGACCAGCGTATCGCCGATCTTCTGTCGG
CCCACAGCATTAACATCGCCCGGCGCACGGTCACCAAGTACCGTGAGATGCTGAGAATAGGATCGTCTTCGGAGCGCAAG
CGGCATTTCTGA

Upstream 100 bases:

>100_bases
CTTTAATCGTTTCAGTATCCATGGTGAGCTACGCCTCAGGCGTCACGGGGCGGGCGTTTCTGATCCTGCAGCATGAAACT
GTTTCTAGGGTGTTCCAGCT

Downstream 100 bases:

>100_bases
GCCGTTGCGCGCCGTATATTATTTTCTTTCGACGTTTCGGGTTGAAGGTTTGCATTTTCGTGATTTAAGAGAATTAAGGG
GCAGGTGACCCGAAACGCAT

Product: sigma-54 (RpoN)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MAIEMRQQMKLTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPVLEETLELEEPKEQDQLEQSEKEETPSGEDNKE
FQEVKAGEETLRETDWDSYLEGYNFSAGEQYYDDEDRPSYENVLTRKGTLGDHLMWQLSLTRFSDVETAVGAEIIGNIDE
DGYFRATVEEIARSQGVDEALVETCLKKVQEFDPMGVGARDLRECLLIQVEQLGMGGSVVEGILRHHLHDLEARKYKQIA
KALGVDINDVLMGARIIAGFDPKPGRVYGSEDVHYISADIFVYKIGEDYVVVLNDEGLPNLRISPYYAGEAKASGQVDAK
AEEYINDKVRSAMWLIKSIHQRQRTIYKVAKSISKFQRDFLDKGIEYLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQ
GLFEMKYFFNSGISTGEGDFIASESVKNKIKEIVEAEDPRKPYSDQRIADLLSAHSINIARRTVTKYREMLRIGSSSERK
RHF

Sequences:

>Translated_483_residues
MAIEMRQQMKLTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPVLEETLELEEPKEQDQLEQSEKEETPSGEDNKE
FQEVKAGEETLRETDWDSYLEGYNFSAGEQYYDDEDRPSYENVLTRKGTLGDHLMWQLSLTRFSDVETAVGAEIIGNIDE
DGYFRATVEEIARSQGVDEALVETCLKKVQEFDPMGVGARDLRECLLIQVEQLGMGGSVVEGILRHHLHDLEARKYKQIA
KALGVDINDVLMGARIIAGFDPKPGRVYGSEDVHYISADIFVYKIGEDYVVVLNDEGLPNLRISPYYAGEAKASGQVDAK
AEEYINDKVRSAMWLIKSIHQRQRTIYKVAKSISKFQRDFLDKGIEYLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQ
GLFEMKYFFNSGISTGEGDFIASESVKNKIKEIVEAEDPRKPYSDQRIADLLSAHSINIARRTVTKYREMLRIGSSSERK
RHF
>Mature_482_residues
AIEMRQQMKLTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPVLEETLELEEPKEQDQLEQSEKEETPSGEDNKEF
QEVKAGEETLRETDWDSYLEGYNFSAGEQYYDDEDRPSYENVLTRKGTLGDHLMWQLSLTRFSDVETAVGAEIIGNIDED
GYFRATVEEIARSQGVDEALVETCLKKVQEFDPMGVGARDLRECLLIQVEQLGMGGSVVEGILRHHLHDLEARKYKQIAK
ALGVDINDVLMGARIIAGFDPKPGRVYGSEDVHYISADIFVYKIGEDYVVVLNDEGLPNLRISPYYAGEAKASGQVDAKA
EEYINDKVRSAMWLIKSIHQRQRTIYKVAKSISKFQRDFLDKGIEYLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQG
LFEMKYFFNSGISTGEGDFIASESVKNKIKEIVEAEDPRKPYSDQRIADLLSAHSINIARRTVTKYREMLRIGSSSERKR
HF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the xylCAB operon and the xylS gene. The open complex (sigma-54 a

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=482, Percent_Identity=39.4190871369295, Blast_Score=333, Evalue=1e-92,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 55247; Mature: 55116

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEMRQQMKLTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPVLEETLELEEPKE
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCH
QDQLEQSEKEETPSGEDNKEFQEVKAGEETLRETDWDSYLEGYNFSAGEQYYDDEDRPSY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCH
ENVLTRKGTLGDHLMWQLSLTRFSDVETAVGAEIIGNIDEDGYFRATVEEIARSQGVDEA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHH
LVETCLKKVQEFDPMGVGARDLRECLLIQVEQLGMGGSVVEGILRHHLHDLEARKYKQIA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALGVDINDVLMGARIIAGFDPKPGRVYGSEDVHYISADIFVYKIGEDYVVVLNDEGLPN
HHHCCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEEEEEEECCCEEEEECCCCCCC
LRISPYYAGEAKASGQVDAKAEEYINDKVRSAMWLIKSIHQRQRTIYKVAKSISKFQRDF
CEECCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKGIEYLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQGLFEMKYFFNSGISTGEGDF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCE
IASESVKNKIKEIVEAEDPRKPYSDQRIADLLSAHSINIARRTVTKYREMLRIGSSSERK
EHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
RHF
CCC
>Mature Secondary Structure 
AIEMRQQMKLTQQLVMTPQLQQAIKLLQLSRLELQDLVRQEMEENPVLEETLELEEPKE
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCH
QDQLEQSEKEETPSGEDNKEFQEVKAGEETLRETDWDSYLEGYNFSAGEQYYDDEDRPSY
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCH
ENVLTRKGTLGDHLMWQLSLTRFSDVETAVGAEIIGNIDEDGYFRATVEEIARSQGVDEA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHH
LVETCLKKVQEFDPMGVGARDLRECLLIQVEQLGMGGSVVEGILRHHLHDLEARKYKQIA
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KALGVDINDVLMGARIIAGFDPKPGRVYGSEDVHYISADIFVYKIGEDYVVVLNDEGLPN
HHHCCCHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEEEEEEECCCEEEEECCCCCCC
LRISPYYAGEAKASGQVDAKAEEYINDKVRSAMWLIKSIHQRQRTIYKVAKSISKFQRDF
CEECCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDKGIEYLRPLVLRDVAEDIGMHESTISRVTTNKYMQTPQGLFEMKYFFNSGISTGEGDF
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCE
IASESVKNKIKEIVEAEDPRKPYSDQRIADLLSAHSINIARRTVTKYREMLRIGSSSERK
EHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHC
RHF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2602128; 12534463 [H]