Click here to switch to the map view.

The map label for this gene is tyrS

Identifier: 78222398

GI number: 78222398

Start: 1308567

End: 1309778

Strand: Direct

Name: tyrS

Synonym: Gmet_1182

Alternate gene names: 78222398

Gene position: 1308567-1309778 (Clockwise)

Preceding gene: 78222397

Following gene: 78222400

Centisome position: 32.74

GC content: 50.08

Gene sequence:

>1212_bases
ATGTCGGTAGCTGAACAGATGGCGATCATCAAGCGCGGAGCAACAGAAATACTCCTTGAGAAAGAACTTGAGGAAAAAAT
CGAAAAATCACTGAGTACCGGGGTTCCGTTGAGAATCAAGGCCGGATTTGATCCAACAGCACCCGACCTCCATCTCGGCC
ATACGGTCCTCTTGCACAAAATGCGTCAGTTTCAGCAGTTGGGTCATGAGGTCTGCTTTCTTATCGGCGACTTCACAGGG
ATGATCGGTGACCCCACAGGCAAGTCGGAGACCCGTAAGGCTCTTACCCGTGAAGATGTTCTGAAAAACGCTGAAACCTA
TAAAGAACAGGTATTCAAGATACTGGATCCGAAGAAGACGCGGGTCGTTTTCAACTCCGAGTGGCTTGGAAAAATGACGG
CCTCCGACATGATCGGGCTTGCGGCACAATCTACAGTAGCGCGGATGCTGGAACGTGACGATTTCGGTAAACGATTTGCC
AATCAACTTCCTATCAGCATTCACGAATTTCTCTACCCACTCATCCAAGGATACGATTCCGTAGCACTCAAGGCTGATGT
TGAACTCGGAGGGACGGATCAAAAGTTCAATCTCCTTGTCGGCCGCGAATTGCAGCGTGTATGGAAACAATCTCCCCAGA
GCGTTATTACCATGCCTCTTCTTGAAGGCCTTGATGGCGTCAACAAGATGAGCAAGTCACTGGGAAACTATATCGGTATT
AATGAGCCTGCCGATGAAATTTTTGGGAAAATCATGTCCATTTCGGACGAACTTATGCTCCGGTACTACGAGCTGCTCAG
CGATCTGACGCTTGCGGAAATCGAAAAACTCAAAGCAGCTATGCGGGATGGTGATGTGCATCCAATGGCAGCTAAGAAGC
AGCTTGCTCGCGAAATAGTTGCTCGTTATCACGGTGCAGTTGCAGCCGATAATGCGGAGGAGAGTTTTGTCAGGCGATTC
CGTGATAATCAAACTCCGGAAGAAATGCCTGAATGCATCTTGTCGGCTGAGGAGGGCAAGGTGCTGCTGGGCAGATTGCT
GGCTGAAGCGGGGCTCGTGAAATCGAACAGTGAGGGACGTCGCGCAATCAACCAGGGCGGCGTGAAGGTGAATGGAGAAA
AGGTCACCAACGATATGCTCGAACTCCCCGGTGTCGGAGAGTACGTCTTGCAGTTTGGCAAGCGGCGTTTCGCGAGAATT
GTCTTTAAGTAA

Upstream 100 bases:

>100_bases
CGTCTGAACGGAGTTGTCCTTGGGATCGATGAGATGAGCGGTTCCGCGCTTTCAGTTGAACGGATTTCACTTACGTGTTC
GTGAGGAGGGAGAGAGTTCC

Downstream 100 bases:

>100_bases
AAACTAATAAAAATATATCTTGACCGTAAGGGGTGGGGGTGGTATAAACGCGTTTCCTTTTGACGGGGCGGGATTGATTG
AGAAGAAAAAACGCTTCGTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSVAEQMAIIKRGATEILLEKELEEKIEKSLSTGVPLRIKAGFDPTAPDLHLGHTVLLHKMRQFQQLGHEVCFLIGDFTG
MIGDPTGKSETRKALTREDVLKNAETYKEQVFKILDPKKTRVVFNSEWLGKMTASDMIGLAAQSTVARMLERDDFGKRFA
NQLPISIHEFLYPLIQGYDSVALKADVELGGTDQKFNLLVGRELQRVWKQSPQSVITMPLLEGLDGVNKMSKSLGNYIGI
NEPADEIFGKIMSISDELMLRYYELLSDLTLAEIEKLKAAMRDGDVHPMAAKKQLAREIVARYHGAVAADNAEESFVRRF
RDNQTPEEMPECILSAEEGKVLLGRLLAEAGLVKSNSEGRRAINQGGVKVNGEKVTNDMLELPGVGEYVLQFGKRRFARI
VFK

Sequences:

>Translated_403_residues
MSVAEQMAIIKRGATEILLEKELEEKIEKSLSTGVPLRIKAGFDPTAPDLHLGHTVLLHKMRQFQQLGHEVCFLIGDFTG
MIGDPTGKSETRKALTREDVLKNAETYKEQVFKILDPKKTRVVFNSEWLGKMTASDMIGLAAQSTVARMLERDDFGKRFA
NQLPISIHEFLYPLIQGYDSVALKADVELGGTDQKFNLLVGRELQRVWKQSPQSVITMPLLEGLDGVNKMSKSLGNYIGI
NEPADEIFGKIMSISDELMLRYYELLSDLTLAEIEKLKAAMRDGDVHPMAAKKQLAREIVARYHGAVAADNAEESFVRRF
RDNQTPEEMPECILSAEEGKVLLGRLLAEAGLVKSNSEGRRAINQGGVKVNGEKVTNDMLELPGVGEYVLQFGKRRFARI
VFK
>Mature_402_residues
SVAEQMAIIKRGATEILLEKELEEKIEKSLSTGVPLRIKAGFDPTAPDLHLGHTVLLHKMRQFQQLGHEVCFLIGDFTGM
IGDPTGKSETRKALTREDVLKNAETYKEQVFKILDPKKTRVVFNSEWLGKMTASDMIGLAAQSTVARMLERDDFGKRFAN
QLPISIHEFLYPLIQGYDSVALKADVELGGTDQKFNLLVGRELQRVWKQSPQSVITMPLLEGLDGVNKMSKSLGNYIGIN
EPADEIFGKIMSISDELMLRYYELLSDLTLAEIEKLKAAMRDGDVHPMAAKKQLAREIVARYHGAVAADNAEESFVRRFR
DNQTPEEMPECILSAEEGKVLLGRLLAEAGLVKSNSEGRRAINQGGVKVNGEKVTNDMLELPGVGEYVLQFGKRRFARIV
FK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr)

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Homo sapiens, GI94681057, Length=416, Percent_Identity=27.6442307692308, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1787925, Length=413, Percent_Identity=26.634382566586, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI25145802, Length=366, Percent_Identity=27.3224043715847, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6325160, Length=382, Percent_Identity=24.3455497382199, Blast_Score=89, Evalue=9e-19,
Organism=Drosophila melanogaster, GI20130383, Length=405, Percent_Identity=26.6666666666667, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYY_GEOMG (Q39WF4)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384145.1
- STRING:   Q39WF4
- GeneID:   3739955
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1182
- NMPDR:   fig|269799.3.peg.1750
- eggNOG:   COG0162
- HOGENOM:   HBG288125
- OMA:   YVVQVGK
- ProtClustDB:   PRK05912
- BioCyc:   GMET269799:GMET_1182-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02007
- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11766
- PRINTS:   PR01040
- SMART:   SM00363
- TIGRFAMs:   TIGR00234

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b

EC number: =6.1.1.1

Molecular weight: Translated: 45026; Mature: 44895

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50889 S4

Important sites: BINDING 232-232

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVAEQMAIIKRGATEILLEKELEEKIEKSLSTGVPLRIKAGFDPTAPDLHLGHTVLLHK
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHH
MRQFQQLGHEVCFLIGDFTGMIGDPTGKSETRKALTREDVLKNAETYKEQVFKILDPKKT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RVVFNSEWLGKMTASDMIGLAAQSTVARMLERDDFGKRFANQLPISIHEFLYPLIQGYDS
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
VALKADVELGGTDQKFNLLVGRELQRVWKQSPQSVITMPLLEGLDGVNKMSKSLGNYIGI
EEEEEECEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCC
NEPADEIFGKIMSISDELMLRYYELLSDLTLAEIEKLKAAMRDGDVHPMAAKKQLAREIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ARYHGAVAADNAEESFVRRFRDNQTPEEMPECILSAEEGKVLLGRLLAEAGLVKSNSEGR
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHH
RAINQGGVKVNGEKVTNDMLELPGVGEYVLQFGKRRFARIVFK
HHHHCCCCEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVAEQMAIIKRGATEILLEKELEEKIEKSLSTGVPLRIKAGFDPTAPDLHLGHTVLLHK
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHH
MRQFQQLGHEVCFLIGDFTGMIGDPTGKSETRKALTREDVLKNAETYKEQVFKILDPKKT
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RVVFNSEWLGKMTASDMIGLAAQSTVARMLERDDFGKRFANQLPISIHEFLYPLIQGYDS
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCC
VALKADVELGGTDQKFNLLVGRELQRVWKQSPQSVITMPLLEGLDGVNKMSKSLGNYIGI
EEEEEECEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCC
NEPADEIFGKIMSISDELMLRYYELLSDLTLAEIEKLKAAMRDGDVHPMAAKKQLAREIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ARYHGAVAADNAEESFVRRFRDNQTPEEMPECILSAEEGKVLLGRLLAEAGLVKSNSEGR
HHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCHHH
RAINQGGVKVNGEKVTNDMLELPGVGEYVLQFGKRRFARIVFK
HHHHCCCCEECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA