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The map label for this gene is prpB

Identifier: 78222338

GI number: 78222338

Start: 1242042

End: 1242956

Strand: Direct

Name: prpB

Synonym: Gmet_1122

Alternate gene names: 78222338

Gene position: 1242042-1242956 (Clockwise)

Preceding gene: 78222337

Following gene: 78222339

Centisome position: 31.07

GC content: 61.75

Gene sequence:

>915_bases
ATGTCTGAGCAGATTTCCGCCGGGAAGCTTTTCCGCAGGGTCATTGATGCTGAAAAGCCGCTTCAGATTGTGGGGACGAT
CAACGCCTACTGCGCAATCCTGGCCGAAAAGGCCGGTCACCGGGCAATCTACCTCTCCGGCGCCGGGGTCGCGAACGCCT
CGTTCGGTCTTCCCGACCTCGGTATAATCTCCCGCAGTGACGTCCTGGAGGAGGTGAGGCGGATTACCGGCGCTTCCCCC
CTGCCGCTCCTGGTCGACATCGATACGGGATGGGGAGAGGAATTCGGGATCGACCGCACGATTAAGGAGATGATTCGCGC
AGGGGCGGCGGCGGTTCACCTTGAGGATCAGGTCGAACCGAAACGCTGCGGCCATCGCCCCAACAAGTCCATTGTTTCAA
CAGGCAGAATGGTGGACCGTATAAAGGCTGCGGTGGATGCCCGAAGCGATGGGGATCTCATGATCATGGCCCGTACCGAC
GCTCTGGCGACTGCAGGGCTCGACGCCGCCGTCGAAAGGGCGGCCCTCTATGTGGAGGCCGGCGCGGAGAGCATCTTTGC
GGAGGGGGTGACCGACCTTGCCATGTACCGGCGGTTTGCCGATGCCGTTGATGTGCCGCTCCTGGCCAATATGACCGAAT
TCGGGAAGACCCCCTACTACACGAAAGAGCAGTTTGCTGCGCAGGGGGTCGCTATGGTGCTCTATCCCCTGAGCGCCTTC
CGGGCCATGAGCAGGGCCGCACTGGAGGTGTATGAGACCATCCTCCGCGCCGGAACCCAGGAGCCGGTTGTGGCGGCCAT
GCAACCCCGGGAGGAACTTTACGAACTGCTCCACTATTACGACTATGAGCGGAAGCTCGACCGGCTGTCTTCCCGTGACG
GCGTTGAAAGCGGCGAGGGGAGGGAGAAACGGTGA

Upstream 100 bases:

>100_bases
GATGAAGAGTTGGCGGAGCTCCTCATGCGGCGCCATATACGGACCGCGCGCATCAGCGTCGAGGAACGGCACAAAAAAGC
CATCGCAAAGGATAGTGGCA

Downstream 100 bases:

>100_bases
GCACGACTGCCGACGTGAATGTCCGGCCAGCCCCGGACCAGGTGCTGGTCGACATAGCCGAGTACGTCACGGGCTACCGG
GTGACGAGCCACGAGGCCCG

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSEQISAGKLFRRVIDAEKPLQIVGTINAYCAILAEKAGHRAIYLSGAGVANASFGLPDLGIISRSDVLEEVRRITGASP
LPLLVDIDTGWGEEFGIDRTIKEMIRAGAAAVHLEDQVEPKRCGHRPNKSIVSTGRMVDRIKAAVDARSDGDLMIMARTD
ALATAGLDAAVERAALYVEAGAESIFAEGVTDLAMYRRFADAVDVPLLANMTEFGKTPYYTKEQFAAQGVAMVLYPLSAF
RAMSRAALEVYETILRAGTQEPVVAAMQPREELYELLHYYDYERKLDRLSSRDGVESGEGREKR

Sequences:

>Translated_304_residues
MSEQISAGKLFRRVIDAEKPLQIVGTINAYCAILAEKAGHRAIYLSGAGVANASFGLPDLGIISRSDVLEEVRRITGASP
LPLLVDIDTGWGEEFGIDRTIKEMIRAGAAAVHLEDQVEPKRCGHRPNKSIVSTGRMVDRIKAAVDARSDGDLMIMARTD
ALATAGLDAAVERAALYVEAGAESIFAEGVTDLAMYRRFADAVDVPLLANMTEFGKTPYYTKEQFAAQGVAMVLYPLSAF
RAMSRAALEVYETILRAGTQEPVVAAMQPREELYELLHYYDYERKLDRLSSRDGVESGEGREKR
>Mature_303_residues
SEQISAGKLFRRVIDAEKPLQIVGTINAYCAILAEKAGHRAIYLSGAGVANASFGLPDLGIISRSDVLEEVRRITGASPL
PLLVDIDTGWGEEFGIDRTIKEMIRAGAAAVHLEDQVEPKRCGHRPNKSIVSTGRMVDRIKAAVDARSDGDLMIMARTDA
LATAGLDAAVERAALYVEAGAESIFAEGVTDLAMYRRFADAVDVPLLANMTEFGKTPYYTKEQFAAQGVAMVLYPLSAFR
AMSRAALEVYETILRAGTQEPVVAAMQPREELYELLHYYDYERKLDRLSSRDGVESGEGREKR

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=292, Percent_Identity=61.986301369863, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1790445, Length=284, Percent_Identity=27.112676056338, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 33249; Mature: 33118

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQISAGKLFRRVIDAEKPLQIVGTINAYCAILAEKAGHRAIYLSGAGVANASFGLPDL
CCCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
GIISRSDVLEEVRRITGASPLPLLVDIDTGWGEEFGIDRTIKEMIRAGAAAVHLEDQVEP
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCH
KRCGHRPNKSIVSTGRMVDRIKAAVDARSDGDLMIMARTDALATAGLDAAVERAALYVEA
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCHHHHHHHHHHHHHH
GAESIFAEGVTDLAMYRRFADAVDVPLLANMTEFGKTPYYTKEQFAAQGVAMVLYPLSAF
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCCHHHHHHCCHHHHHHHHHHH
RAMSRAALEVYETILRAGTQEPVVAAMQPREELYELLHYYDYERKLDRLSSRDGVESGEG
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
REKR
CCCC
>Mature Secondary Structure 
SEQISAGKLFRRVIDAEKPLQIVGTINAYCAILAEKAGHRAIYLSGAGVANASFGLPDL
CCCCHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
GIISRSDVLEEVRRITGASPLPLLVDIDTGWGEEFGIDRTIKEMIRAGAAAVHLEDQVEP
CCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHCCCCEEEECCCCCH
KRCGHRPNKSIVSTGRMVDRIKAAVDARSDGDLMIMARTDALATAGLDAAVERAALYVEA
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHCHHHHHHHHHHHHHH
GAESIFAEGVTDLAMYRRFADAVDVPLLANMTEFGKTPYYTKEQFAAQGVAMVLYPLSAF
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHCHHHHCCCCCCCHHHHHHCCHHHHHHHHHHH
RAMSRAALEVYETILRAGTQEPVVAAMQPREELYELLHYYDYERKLDRLSSRDGVESGEG
HHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
REKR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]