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The map label for this gene is hoxH [H]

Identifier: 78222331

GI number: 78222331

Start: 1235484

End: 1236914

Strand: Direct

Name: hoxH [H]

Synonym: Gmet_1113

Alternate gene names: 78222331

Gene position: 1235484-1236914 (Clockwise)

Preceding gene: 78222330

Following gene: 78222332

Centisome position: 30.91

GC content: 66.95

Gene sequence:

>1431_bases
ATGTCACGCACCATTACCATAGACCCCGTCACCCGCATCGAGGGGCATGCCACCATCACCATCCGGCTCGATGATGCCGG
CTCCGTGGCGGACGCCCGGCTCCACGTCACCGAGTTCCGGGGCTTCGAGGCCTTCTGCGTAGGGCGGAGCATCTGGGAGA
TGCCGGGCATAACGGCCCGCATCTGCGGCATCTGCCCCATCAGCCACTCCATCGCCTCCGCCCGGGCCGGCGACGCCATC
CTCGGCGTGGATATCCCCCCGGCCGCAGAGCAGCTGCGGCGCATCGCCAACCTGGCCTCCCTCATCCAGAGCCACGCCCT
CAGCTTTTTCCACCTCTGCGCCGCCGATCTCCTCCTGGGGATGGACCACGACCCGTCCCGCCGCAGCCTCTGGGGGCTTC
TGGAGAGTCGCCCCGACGTGGCCCGGCGCGGTATCAGGCTGCGGCAGATCGGCCAGCAGATCGTGAGCAACCTGGCGGGT
GAGAGGGTCCACCCCTCCTGGGCCGTCCCCGGTGGGGTCCGGGAGCCCCTTGATGCCGAGCGGCGCCGGAAGGTCGTCGA
ACTACTCCCCGAGGGAATTGCCATCATCCGCTCGGCTTTCGAGCTGCTCGACGAGATCTACGGACGCCATGGCGCCGAGG
TCGGCACCTACGGCGATTTTCCCTCCCTGTTCGCCGGCCTTGTGGGGGAGGACGGAGGACTCGAGCACTACGACGGCATG
CTCCGCTTTACCGACAGCTCCGGTGCCGTGGTGGAGGAGGTCCCCCCTGACCACTACGACCTCGCCATCGGCGAGAGGAG
CGAACCGTGGAGCTACATGAAATTCCCTTTCTACCGCAACCATGCCGGGCGTGATGGGGGAGGGATGTTCCGGGTGGGGC
CCCTGGCCCGTCTTAACCTCTGCGAACGGGCCGGCACCCCCGAGGCTGACCGGGAACTGCAGACATTCCGGCAGCTGCGG
GGCACGGGAGGGGTGGTCACCGGCAGCTTCCACTACCACCATGCCCGGCTCATAGAGATCCTCCACGCCCTGGAGCGGAT
TGGCGAGTTGCTGGACGATCCCGCCATCCTTGACCGTCACGTCCGGAGCGAGGCGGGGGTCAACCGCCGACACGGCGTCG
GCTTCTGCGAAGCCCCCCGGGGGACCCTCTTCCACGACTACGAGGTGGACGACGACGGCCTCATTCTGCGCCTCAACCTC
CTCATTGCCACCGGGCAGAACAACCTGGCCATGAACCGGACCATCCTGGAGGTGGCCAGGTCGCATATCAAGGGGGGGAA
AGTGAGCGAGGGGATTCTGAACCGGATCGAGCACGGCATCCGCGCCTACGACCCGTGCCTCTCCTGCGCCACTCACGTCT
ACGGCAGAATGCCGTTCCAGGTGTTGCTCCTGGCGCCGGATGGGACGGTGGTGGATGAGATAGCGAGCTAA

Upstream 100 bases:

>100_bases
CCCCCGGACCCGGAGAGGATCTGGACCGCCATCGGGTGCCTCCTGCGGGGGGAACCGGTTCATCTCGAATCAGATATGCT
CACCTTCGGATAGGATTGCC

Downstream 100 bases:

>100_bases
CCCTGGCGGTTTCCCCCAAGAGCAACCACTTCTCAAGATCAATCCGCGACCTTGGCATCATCGATTCCACGGTGCGTGAA
GCCAGACTCTTAGCCTGTGG

Product: nickel-dependent hydrogenase, large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSRTITIDPVTRIEGHATITIRLDDAGSVADARLHVTEFRGFEAFCVGRSIWEMPGITARICGICPISHSIASARAGDAI
LGVDIPPAAEQLRRIANLASLIQSHALSFFHLCAADLLLGMDHDPSRRSLWGLLESRPDVARRGIRLRQIGQQIVSNLAG
ERVHPSWAVPGGVREPLDAERRRKVVELLPEGIAIIRSAFELLDEIYGRHGAEVGTYGDFPSLFAGLVGEDGGLEHYDGM
LRFTDSSGAVVEEVPPDHYDLAIGERSEPWSYMKFPFYRNHAGRDGGGMFRVGPLARLNLCERAGTPEADRELQTFRQLR
GTGGVVTGSFHYHHARLIEILHALERIGELLDDPAILDRHVRSEAGVNRRHGVGFCEAPRGTLFHDYEVDDDGLILRLNL
LIATGQNNLAMNRTILEVARSHIKGGKVSEGILNRIEHGIRAYDPCLSCATHVYGRMPFQVLLLAPDGTVVDEIAS

Sequences:

>Translated_476_residues
MSRTITIDPVTRIEGHATITIRLDDAGSVADARLHVTEFRGFEAFCVGRSIWEMPGITARICGICPISHSIASARAGDAI
LGVDIPPAAEQLRRIANLASLIQSHALSFFHLCAADLLLGMDHDPSRRSLWGLLESRPDVARRGIRLRQIGQQIVSNLAG
ERVHPSWAVPGGVREPLDAERRRKVVELLPEGIAIIRSAFELLDEIYGRHGAEVGTYGDFPSLFAGLVGEDGGLEHYDGM
LRFTDSSGAVVEEVPPDHYDLAIGERSEPWSYMKFPFYRNHAGRDGGGMFRVGPLARLNLCERAGTPEADRELQTFRQLR
GTGGVVTGSFHYHHARLIEILHALERIGELLDDPAILDRHVRSEAGVNRRHGVGFCEAPRGTLFHDYEVDDDGLILRLNL
LIATGQNNLAMNRTILEVARSHIKGGKVSEGILNRIEHGIRAYDPCLSCATHVYGRMPFQVLLLAPDGTVVDEIAS
>Mature_475_residues
SRTITIDPVTRIEGHATITIRLDDAGSVADARLHVTEFRGFEAFCVGRSIWEMPGITARICGICPISHSIASARAGDAIL
GVDIPPAAEQLRRIANLASLIQSHALSFFHLCAADLLLGMDHDPSRRSLWGLLESRPDVARRGIRLRQIGQQIVSNLAGE
RVHPSWAVPGGVREPLDAERRRKVVELLPEGIAIIRSAFELLDEIYGRHGAEVGTYGDFPSLFAGLVGEDGGLEHYDGML
RFTDSSGAVVEEVPPDHYDLAIGERSEPWSYMKFPFYRNHAGRDGGGMFRVGPLARLNLCERAGTPEADRELQTFRQLRG
TGGVVTGSFHYHHARLIEILHALERIGELLDDPAILDRHVRSEAGVNRRHGVGFCEAPRGTLFHDYEVDDDGLILRLNLL
IATGQNNLAMNRTILEVARSHIKGGKVSEGILNRIEHGIRAYDPCLSCATHVYGRMPFQVLLLAPDGTVVDEIAS

Specific function: This Is One Of Three E.Coli Hydrogenases Synthesized In Response To Different Physiological Conditions. Hyd2 Is Involved In Hydrogen Uptake. [C]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1789368, Length=557, Percent_Identity=22.6211849192101, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787207, Length=181, Percent_Identity=29.8342541436464, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.1.2 [H]

Molecular weight: Translated: 52332; Mature: 52201

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTITIDPVTRIEGHATITIRLDDAGSVADARLHVTEFRGFEAFCVGRSIWEMPGITAR
CCCEEEECCCCEECCCEEEEEEECCCCCCCHHEEEEEHHCCCEEEECCCHHHHCCCCHHH
ICGICPISHSIASARAGDAILGVDIPPAAEQLRRIANLASLIQSHALSFFHLCAADLLLG
HHCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MDHDPSRRSLWGLLESRPDVARRGIRLRQIGQQIVSNLAGERVHPSWAVPGGVREPLDAE
CCCCCCHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
RRRKVVELLPEGIAIIRSAFELLDEIYGRHGAEVGTYGDFPSLFAGLVGEDGGLEHYDGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHCCE
LRFTDSSGAVVEEVPPDHYDLAIGERSEPWSYMKFPFYRNHAGRDGGGMFRVGPLARLNL
EEEECCCCCEEECCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCCEEEECCHHHHHH
CERAGTPEADRELQTFRQLRGTGGVVTGSFHYHHARLIEILHALERIGELLDDPAILDRH
HHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VRSEAGVNRRHGVGFCEAPRGTLFHDYEVDDDGLILRLNLLIATGQNNLAMNRTILEVAR
HHHHCCCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEEEEEEECCCCCHHHHHHHHHHH
SHIKGGKVSEGILNRIEHGIRAYDPCLSCATHVYGRMPFQVLLLAPDGTVVDEIAS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCC
>Mature Secondary Structure 
SRTITIDPVTRIEGHATITIRLDDAGSVADARLHVTEFRGFEAFCVGRSIWEMPGITAR
CCEEEECCCCEECCCEEEEEEECCCCCCCHHEEEEEHHCCCEEEECCCHHHHCCCCHHH
ICGICPISHSIASARAGDAILGVDIPPAAEQLRRIANLASLIQSHALSFFHLCAADLLLG
HHCCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
MDHDPSRRSLWGLLESRPDVARRGIRLRQIGQQIVSNLAGERVHPSWAVPGGVREPLDAE
CCCCCCHHHHHHHHHCCHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHH
RRRKVVELLPEGIAIIRSAFELLDEIYGRHGAEVGTYGDFPSLFAGLVGEDGGLEHYDGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHCCE
LRFTDSSGAVVEEVPPDHYDLAIGERSEPWSYMKFPFYRNHAGRDGGGMFRVGPLARLNL
EEEECCCCCEEECCCCCCEEEEECCCCCCCCCEECCCCCCCCCCCCCCEEEECCHHHHHH
CERAGTPEADRELQTFRQLRGTGGVVTGSFHYHHARLIEILHALERIGELLDDPAILDRH
HHHCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VRSEAGVNRRHGVGFCEAPRGTLFHDYEVDDDGLILRLNLLIATGQNNLAMNRTILEVAR
HHHHCCCCCCCCCCEEECCCCCEEEEEEECCCCEEEEEEEEEEECCCCCHHHHHHHHHHH
SHIKGGKVSEGILNRIEHGIRAYDPCLSCATHVYGRMPFQVLLLAPDGTVVDEIAS
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2188945; 12948488; 2496982 [H]