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The map label for this gene is yrhE [H]

Identifier: 78222329

GI number: 78222329

Start: 1234205

End: 1234921

Strand: Direct

Name: yrhE [H]

Synonym: Gmet_1111

Alternate gene names: 78222329

Gene position: 1234205-1234921 (Clockwise)

Preceding gene: 78222328

Following gene: 78222330

Centisome position: 30.88

GC content: 67.78

Gene sequence:

>717_bases
ATGTCGGTCGTGACCCTGACCATCGACGGCGAGCTGGTGAGCGGCCGGCGGGGGGAGACGCTCCTCCAGGTGATCCGCGA
GCACGGCTTGGCGCTTCCCACCCTCTGTCACCTGGAGGGGCTTTCCGAGCGGGGGGGATGCCGGCTCTGCATCGTGGAGG
TGGAGGGAAGCCCCCGCCCCATGCCCGCCTGCAGCACCGCCGCCCGGGAGGGGATGGTGGTCCGGACCGCCACCGACCGG
CTCATCCGCTACCGCCGCATGATCGTGGAACTCCTCTTTGCCGAGCGCAACCACTACTGCGCCGTCTGCGTCTCCGCCGG
AAACTGCGAGCTCCAGACGGTGGCAGCCACTCTGGGGGTCGACCATGTCCGCTACGAGTACCTCTCCCCCCGGCTCACCA
TGGACCTCTCCCACGAGCGGTTCGGCATCGACCACAACCGCTGCATCCTCTGCACCCGCTGCGTGAGGGTCTGCGACGAG
GTCGAAGGGGTCCATACCTGGGATGTCTCCTGCCGTGGAGTCCGGAGCCGGGTCATCGCCGACCTGAACCATCCCTGGGG
GCGGAGCGAAACCTGCACCAGTTGCGGCAAGTGCGTCCAGGTCTGCCCCACCGGCGCCCTCTTCACCAAAGGGGCAGCGG
TGGGCGAGATGGTGAAGGATGCCGGCATGCTCACGCGGGTTATCGAGGGACGGGACAAGAAGGGATGGCAGGAATGA

Upstream 100 bases:

>100_bases
ACCGTTGCGCGGCCGGCGTCTGCTCCATGGCGGAAGTGCCCGCCGGCCCCGAGCAGGAGGCGCTGCTGCACGCCATGGGT
GGTGCCGGGGAGGAGGCGTG

Downstream 100 bases:

>100_bases
CGGGGGGCGGCAATGGGTAGGCGGGGAAGGATCCGGTTCGCCACGGTCTGGCTCGGTGGCTGCTCGGGGTGCCACATGAG
CTTTCTCGATCTGGACGAGC

Product: bidirectional hydrogenase complex protein HoxU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSVVTLTIDGELVSGRRGETLLQVIREHGLALPTLCHLEGLSERGGCRLCIVEVEGSPRPMPACSTAAREGMVVRTATDR
LIRYRRMIVELLFAERNHYCAVCVSAGNCELQTVAATLGVDHVRYEYLSPRLTMDLSHERFGIDHNRCILCTRCVRVCDE
VEGVHTWDVSCRGVRSRVIADLNHPWGRSETCTSCGKCVQVCPTGALFTKGAAVGEMVKDAGMLTRVIEGRDKKGWQE

Sequences:

>Translated_238_residues
MSVVTLTIDGELVSGRRGETLLQVIREHGLALPTLCHLEGLSERGGCRLCIVEVEGSPRPMPACSTAAREGMVVRTATDR
LIRYRRMIVELLFAERNHYCAVCVSAGNCELQTVAATLGVDHVRYEYLSPRLTMDLSHERFGIDHNRCILCTRCVRVCDE
VEGVHTWDVSCRGVRSRVIADLNHPWGRSETCTSCGKCVQVCPTGALFTKGAAVGEMVKDAGMLTRVIEGRDKKGWQE
>Mature_237_residues
SVVTLTIDGELVSGRRGETLLQVIREHGLALPTLCHLEGLSERGGCRLCIVEVEGSPRPMPACSTAAREGMVVRTATDRL
IRYRRMIVELLFAERNHYCAVCVSAGNCELQTVAATLGVDHVRYEYLSPRLTMDLSHERFGIDHNRCILCTRCVRVCDEV
EGVHTWDVSCRGVRSRVIADLNHPWGRSETCTSCGKCVQVCPTGALFTKGAAVGEMVKDAGMLTRVIEGRDKKGWQE

Specific function: Ndh-1 Shuttles Electrons From NADH, Via Fmn And Iron- Sulfur (Fe-S) Centers, To Quinones In The Respiratory Chain. The Immediate Electron Acceptor For The Enzyme In This Species Is Believed To Be Ubiquinone. Couples The Redox Reaction To Proton Translocat

COG id: COG1034

COG function: function code C; NADH dehydrogenase/NADH:ubiquinone oxidoreductase 75 kD subunit (chain G)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI33519475, Length=222, Percent_Identity=29.7297297297297, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI145693161, Length=223, Percent_Identity=29.1479820627803, Blast_Score=83, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17565758, Length=216, Percent_Identity=31.9444444444444, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI193209088, Length=217, Percent_Identity=31.7972350230415, Blast_Score=100, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI32566231, Length=217, Percent_Identity=31.7972350230415, Blast_Score=100, Evalue=8e-22,
Organism=Drosophila melanogaster, GI24640559, Length=205, Percent_Identity=30.2439024390244, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24640557, Length=205, Percent_Identity=30.2439024390244, Blast_Score=103, Evalue=7e-23,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR009010
- InterPro:   IPR012675
- InterPro:   IPR001041
- InterPro:   IPR006478
- InterPro:   IPR012285
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006963
- InterPro:   IPR019574 [H]

Pfam domain/function: PF04879 Molybdop_Fe4S4; PF00384 Molybdopterin; PF01568 Molydop_binding; PF10588 NADH-G_4Fe-4S_3 [H]

EC number: =1.2.1.2 [H]

Molecular weight: Translated: 26306; Mature: 26175

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: PS51085 2FE2S_FER_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.7 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
9.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVTLTIDGELVSGRRGETLLQVIREHGLALPTLCHLEGLSERGGCRLCIVEVEGSPRP
CEEEEEEECCEEECCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEEEECCCCCC
MPACSTAAREGMVVRTATDRLIRYRRMIVELLFAERNHYCAVCVSAGNCELQTVAATLGV
CCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCH
DHVRYEYLSPRLTMDLSHERFGIDHNRCILCTRCVRVCDEVEGVHTWDVSCRGVRSRVIA
HHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
DLNHPWGRSETCTSCGKCVQVCPTGALFTKGAAVGEMVKDAGMLTRVIEGRDKKGWQE
HCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVVTLTIDGELVSGRRGETLLQVIREHGLALPTLCHLEGLSERGGCRLCIVEVEGSPRP
EEEEEEECCEEECCCCCHHHHHHHHHCCCCCCHHHHHCCCCCCCCEEEEEEEECCCCCC
MPACSTAAREGMVVRTATDRLIRYRRMIVELLFAERNHYCAVCVSAGNCELQTVAATLGV
CCCHHHHHHCCEEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHCH
DHVRYEYLSPRLTMDLSHERFGIDHNRCILCTRCVRVCDEVEGVHTWDVSCRGVRSRVIA
HHHHHHHCCCEEEEECCHHHCCCCCCCCHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHH
DLNHPWGRSETCTSCGKCVQVCPTGALFTKGAAVGEMVKDAGMLTRVIEGRDKKGWQE
HCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]