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The map label for this gene is aegA [H]

Identifier: 78188515

GI number: 78188515

Start: 640802

End: 642244

Strand: Reverse

Name: aegA [H]

Synonym: Cag_0537

Alternate gene names: 78188515

Gene position: 642244-640802 (Counterclockwise)

Preceding gene: 162319774

Following gene: 78188514

Centisome position: 24.97

GC content: 47.96

Gene sequence:

>1443_bases
ATGACTATGGACGCAAAAACAATTACCACCAAACAGCGCCTTGCCATTCCTCGTCAGGCAATGCCAGCTCAAGATCCAGC
GGTTCGGACACACAATTTTTTAGAAGTAAACCTTGGCTATACGCCTGAACTTGCCCAGCAAGAGGCGTTGCGCTGCATTC
AGTGCAAAGACCCTGTCTGCATTAAGGGGTGCCCTGTTAATATTAAGATTGACCAGTTTATTAAGCTAATTGCTGAGGGC
GATTTTTTGGGTGCCGCTCGCAAAATTAAAGAGGATAACGTGCTGCCTGCTATTTGCGGACGAGTTTGCCCGCAAGAAGA
TCAGTGCGAAAAAGTGTGCGTGCTTACGAAAAAATATACCCCTATTGCTATTGGCAACCTTGAGCGCTTTGCGGCTGACT
ACGAGCGTGAGCATGGTGATATTGAGCTACCATCGGTAAAAGCGCCAACGGGCAAACGTGTGGCTGTTATTGGGAGCGGT
CCAGCAGGGTTAAGCTGCGCCAACGACCTCATTCAATTAGGGCACGACGTTACGGTATTTGAAGCTTTACACGAGCTTGG
TGGCGTGCTGATGTATGGCATTCCAGAATTCCGCCTTCCAAAAGAGATTGTTCGCACCGAAATTGACGGCTTAAAAAAGC
TTGGGGTGAAGTTTGTTACCAACACCGTTGTAGGACGCTCAGTTACGGTTGATGAGTTAATGGAAGAGGAAAATTTTGAT
GCCATTTTTATTGGCGTTGGTGCAGGCTTACCATGGTTTATGGGCATTCCGGGCGAAAACCTGCTTGGTGTTTATTCAGC
CAACGAGTTCCTGACGCGCGTTAACTTGATGAAATCGTACAACTTTCCTGATAACGATACACCTGTATTTAATTGCGAAG
GCAAAAATGTTGCCGTATTTGGCGGTGGCAATACTGCAATGGATGCGGTTCGTACAGCAAAACGGCTTGGTGCAAAAAAT
GCCTACATTGTTTACCGTCGCTCGGAAGCTGAAATGCCTGCCCGTATTGAAGAGGTGCACCATGCCCGCTCGGAAGGCAT
TGAATTTTTAATGCTGATGAATCCGCTTGAATTTATTGGCAACGATGAGCAGTGGCTAACAGGCGCAAAATGCCTTCGTA
TGGAACTTGGTGAACCTGACGATTCAGGGCGTCGTCGTCCCGTTCCCATCAAAGATTCAGAATTTATTTTGCCGATTGAC
ATGGCAGTTATCTCAATTGGCAACGGATCCAATCCGCTGATTAAGCAAACCACGCCCGATATTTCGGTAAGCAAGCGCGA
CACTATTGTCGTTGACCTCAACACGATGGCAACCTCTAAAGAGAACGTTTATGCTGGTGGCGATATTGTAACCGGTGGCG
CTACGGTAATTCTTGCTATGGGCGCAGGACGCACAGCCGCAGCGGCTATTCACGAAAAGCTCATGGGTGGCAGCGCTTCA
TAA

Upstream 100 bases:

>100_bases
CATTATCTCCCGCAAGAGAAAAGCTCACTTGAGGCATTTGCAGAACATAAATGCCACCTTCAACAATCACTTTAACCGCT
ATTTTGCATAGAAAGACATT

Downstream 100 bases:

>100_bases
CCAACGTCACCTATCAACAGCAAAGGGCATTGCCCATTTTTCACCACCCCGCCATTTTAAAAAGCGGGGTGTTTTTTTGT
ACGTCCCTTTATTTCTTTAT

Product: putative oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MTMDAKTITTKQRLAIPRQAMPAQDPAVRTHNFLEVNLGYTPELAQQEALRCIQCKDPVCIKGCPVNIKIDQFIKLIAEG
DFLGAARKIKEDNVLPAICGRVCPQEDQCEKVCVLTKKYTPIAIGNLERFAADYEREHGDIELPSVKAPTGKRVAVIGSG
PAGLSCANDLIQLGHDVTVFEALHELGGVLMYGIPEFRLPKEIVRTEIDGLKKLGVKFVTNTVVGRSVTVDELMEEENFD
AIFIGVGAGLPWFMGIPGENLLGVYSANEFLTRVNLMKSYNFPDNDTPVFNCEGKNVAVFGGGNTAMDAVRTAKRLGAKN
AYIVYRRSEAEMPARIEEVHHARSEGIEFLMLMNPLEFIGNDEQWLTGAKCLRMELGEPDDSGRRRPVPIKDSEFILPID
MAVISIGNGSNPLIKQTTPDISVSKRDTIVVDLNTMATSKENVYAGGDIVTGGATVILAMGAGRTAAAAIHEKLMGGSAS

Sequences:

>Translated_480_residues
MTMDAKTITTKQRLAIPRQAMPAQDPAVRTHNFLEVNLGYTPELAQQEALRCIQCKDPVCIKGCPVNIKIDQFIKLIAEG
DFLGAARKIKEDNVLPAICGRVCPQEDQCEKVCVLTKKYTPIAIGNLERFAADYEREHGDIELPSVKAPTGKRVAVIGSG
PAGLSCANDLIQLGHDVTVFEALHELGGVLMYGIPEFRLPKEIVRTEIDGLKKLGVKFVTNTVVGRSVTVDELMEEENFD
AIFIGVGAGLPWFMGIPGENLLGVYSANEFLTRVNLMKSYNFPDNDTPVFNCEGKNVAVFGGGNTAMDAVRTAKRLGAKN
AYIVYRRSEAEMPARIEEVHHARSEGIEFLMLMNPLEFIGNDEQWLTGAKCLRMELGEPDDSGRRRPVPIKDSEFILPID
MAVISIGNGSNPLIKQTTPDISVSKRDTIVVDLNTMATSKENVYAGGDIVTGGATVILAMGAGRTAAAAIHEKLMGGSAS
>Mature_479_residues
TMDAKTITTKQRLAIPRQAMPAQDPAVRTHNFLEVNLGYTPELAQQEALRCIQCKDPVCIKGCPVNIKIDQFIKLIAEGD
FLGAARKIKEDNVLPAICGRVCPQEDQCEKVCVLTKKYTPIAIGNLERFAADYEREHGDIELPSVKAPTGKRVAVIGSGP
AGLSCANDLIQLGHDVTVFEALHELGGVLMYGIPEFRLPKEIVRTEIDGLKKLGVKFVTNTVVGRSVTVDELMEEENFDA
IFIGVGAGLPWFMGIPGENLLGVYSANEFLTRVNLMKSYNFPDNDTPVFNCEGKNVAVFGGGNTAMDAVRTAKRLGAKNA
YIVYRRSEAEMPARIEEVHHARSEGIEFLMLMNPLEFIGNDEQWLTGAKCLRMELGEPDDSGRRRPVPIKDSEFILPIDM
AVISIGNGSNPLIKQTTPDISVSKRDTIVVDLNTMATSKENVYAGGDIVTGGATVILAMGAGRTAAAAIHEKLMGGSAS

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=452, Percent_Identity=32.5221238938053, Blast_Score=217, Evalue=2e-56,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=44.4444444444444, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788811, Length=448, Percent_Identity=38.6160714285714, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1789606, Length=478, Percent_Identity=37.0292887029289, Blast_Score=301, Evalue=6e-83,
Organism=Escherichia coli, GI87082180, Length=444, Percent_Identity=37.3873873873874, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1788468, Length=454, Percent_Identity=32.5991189427313, Blast_Score=207, Evalue=8e-55,
Organism=Escherichia coli, GI1789243, Length=438, Percent_Identity=32.4200913242009, Blast_Score=168, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71984108, Length=447, Percent_Identity=31.3199105145414, Blast_Score=182, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17570289, Length=485, Percent_Identity=28.2474226804124, Blast_Score=174, Evalue=7e-44,
Organism=Caenorhabditis elegans, GI17543860, Length=177, Percent_Identity=30.5084745762712, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=485, Percent_Identity=32.3711340206186, Blast_Score=209, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24640763, Length=454, Percent_Identity=33.2599118942731, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI18858217, Length=454, Percent_Identity=33.2599118942731, Blast_Score=204, Evalue=1e-52,
Organism=Drosophila melanogaster, GI28574881, Length=489, Percent_Identity=29.038854805726, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24665539, Length=489, Percent_Identity=29.038854805726, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24665547, Length=489, Percent_Identity=29.038854805726, Blast_Score=196, Evalue=3e-50,
Organism=Drosophila melanogaster, GI24665543, Length=489, Percent_Identity=29.038854805726, Blast_Score=196, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006 [H]

Pfam domain/function: PF00037 Fer4; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 52166; Mature: 52035

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMDAKTITTKQRLAIPRQAMPAQDPAVRTHNFLEVNLGYTPELAQQEALRCIQCKDPVC
CCCCCCHHCCHHHHCCCHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEE
IKGCPVNIKIDQFIKLIAEGDFLGAARKIKEDNVLPAICGRVCPQEDQCEKVCVLTKKYT
EECCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHCCCEEEEECCCC
PIAIGNLERFAADYEREHGDIELPSVKAPTGKRVAVIGSGPAGLSCANDLIQLGHDVTVF
CEEECCHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHH
EALHELGGVLMYGIPEFRLPKEIVRTEIDGLKKLGVKFVTNTVVGRSVTVDELMEEENFD
HHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHCCCCC
AIFIGVGAGLPWFMGIPGENLLGVYSANEFLTRVNLMKSYNFPDNDTPVFNCEGKNVAVF
EEEEECCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEE
GGGNTAMDAVRTAKRLGAKNAYIVYRRSEAEMPARIEEVHHARSEGIEFLMLMNPLEFIG
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHC
NDEQWLTGAKCLRMELGEPDDSGRRRPVPIKDSEFILPIDMAVISIGNGSNPLIKQTTPD
CCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCCCC
ISVSKRDTIVVDLNTMATSKENVYAGGDIVTGGATVILAMGAGRTAAAAIHEKLMGGSAS
CEECCCCEEEEEECCCCCCCCCEEECCCEEECCEEEEEEECCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TMDAKTITTKQRLAIPRQAMPAQDPAVRTHNFLEVNLGYTPELAQQEALRCIQCKDPVC
CCCCCHHCCHHHHCCCHHCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEE
IKGCPVNIKIDQFIKLIAEGDFLGAARKIKEDNVLPAICGRVCPQEDQCEKVCVLTKKYT
EECCCCEEEHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHCCCCCHHHCCCEEEEECCCC
PIAIGNLERFAADYEREHGDIELPSVKAPTGKRVAVIGSGPAGLSCANDLIQLGHDVTVF
CEEECCHHHHHHHHHHCCCCEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHH
EALHELGGVLMYGIPEFRLPKEIVRTEIDGLKKLGVKFVTNTVVGRSVTVDELMEEENFD
HHHHHHCCEEEECCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCHHHHHHCCCCC
AIFIGVGAGLPWFMGIPGENLLGVYSANEFLTRVNLMKSYNFPDNDTPVFNCEGKNVAVF
EEEEECCCCCCEEECCCCCCEEEEECHHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEE
GGGNTAMDAVRTAKRLGAKNAYIVYRRSEAEMPARIEEVHHARSEGIEFLMLMNPLEFIG
CCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEECHHHHHC
NDEQWLTGAKCLRMELGEPDDSGRRRPVPIKDSEFILPIDMAVISIGNGSNPLIKQTTPD
CCHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCEECCCCC
ISVSKRDTIVVDLNTMATSKENVYAGGDIVTGGATVILAMGAGRTAAAAIHEKLMGGSAS
CEECCCCEEEEEECCCCCCCCCEEECCCEEECCEEEEEEECCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8955321; 9205837; 9278503 [H]