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The map label for this gene is nfo

Identifier: 78188506

GI number: 78188506

Start: 616283

End: 617131

Strand: Reverse

Name: nfo

Synonym: Cag_0528

Alternate gene names: 78188506

Gene position: 617131-616283 (Counterclockwise)

Preceding gene: 78188507

Following gene: 78188503

Centisome position: 23.99

GC content: 47.23

Gene sequence:

>849_bases
ATGAAACGAGTTGGAGCACATGTTAGTGCAAGTGGTGGCGTTGAACAAGCACCGTTAAACGCTACGGCAATTGGCGCTAA
AGCTTTTGCGCTGTTTACCAAAAATCAACGTCAGTGGAAGGCGCCAAAACTTAGTAAAGCTACCATTGAAGCCTTTCAAA
AAGCATGTGCCGATGGAGGCTTTCAGCCGCAACACATTTTACCGCACGATAGCTACCTTATTAATCTTGGTAGCCCCGAT
CCCGAAAAGTTGGAACGCGCTCGTTCAGCATTTATTGATGAAATGCAGCGCGTAGCCGACCTTGGCTTACAGCTTTTAAA
CTTCCACCCGGGCAGCCATCTTAAAGAGATTTCCGAAGAGGCAAGTTTACTCTTGATTGCAGAATCCATTAACATGGCGC
TTGAGGCAACCAATGGCGTAACTGCCGTGATTGAAAACACCGCAGGACAAGGTACCAACCTTGGTTACCGCTTTGAGCAA
ATTGCTTTTTTAATTGATCGAATTGAAGATAAAAGCCGTGTTGGCGTTTGCCTCGATACCTGCCACCTTTTTGCCAGCGG
CTACGACCTTAGTAGCACCGAAGCTATTGAAACCACCTTCAATGAATTTGATAGCACCGTTGGTTTGCACTATTTGCGGG
GAATGCACCTCAACGATGCCATGCAACCACTTGGCAGCCGCGTTGATCGCCACGCCAGCCTTGGCAAAGGCACCATTGGC
ATGGCAGCATTCACCTTTATTATGAATCATCCCGCTTGTGAGGAAATTCCACTGATTCTTGAAACTCCCAATCCCGACAT
TTGGAGCGAAGAGATTGCTCTTCTTTACTCGCTTCAGCAAGTAGATTGA

Upstream 100 bases:

>100_bases
AGGATTAAGTAAATTTTACAGCAAGGTAGTCACTTATCCTATCCGACCCACAACATACTGCAAAGAGCAACATAGAAATT
ATCCTTTAGAGAGTTGACTT

Downstream 100 bases:

>100_bases
AAGCATTTCCCCCGCAAACAAAAAATCCCCTCATAGCTTATAACCATGAGGGGATTTTCTTTCTCTCTTGTTTTTAATCC
CGAAGGGATTCAATATGAAT

Product: endonuclease IV

Products: NA

Alternate protein names: Endodeoxyribonuclease IV; Endonuclease IV

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MKRVGAHVSASGGVEQAPLNATAIGAKAFALFTKNQRQWKAPKLSKATIEAFQKACADGGFQPQHILPHDSYLINLGSPD
PEKLERARSAFIDEMQRVADLGLQLLNFHPGSHLKEISEEASLLLIAESINMALEATNGVTAVIENTAGQGTNLGYRFEQ
IAFLIDRIEDKSRVGVCLDTCHLFASGYDLSSTEAIETTFNEFDSTVGLHYLRGMHLNDAMQPLGSRVDRHASLGKGTIG
MAAFTFIMNHPACEEIPLILETPNPDIWSEEIALLYSLQQVD

Sequences:

>Translated_282_residues
MKRVGAHVSASGGVEQAPLNATAIGAKAFALFTKNQRQWKAPKLSKATIEAFQKACADGGFQPQHILPHDSYLINLGSPD
PEKLERARSAFIDEMQRVADLGLQLLNFHPGSHLKEISEEASLLLIAESINMALEATNGVTAVIENTAGQGTNLGYRFEQ
IAFLIDRIEDKSRVGVCLDTCHLFASGYDLSSTEAIETTFNEFDSTVGLHYLRGMHLNDAMQPLGSRVDRHASLGKGTIG
MAAFTFIMNHPACEEIPLILETPNPDIWSEEIALLYSLQQVD
>Mature_282_residues
MKRVGAHVSASGGVEQAPLNATAIGAKAFALFTKNQRQWKAPKLSKATIEAFQKACADGGFQPQHILPHDSYLINLGSPD
PEKLERARSAFIDEMQRVADLGLQLLNFHPGSHLKEISEEASLLLIAESINMALEATNGVTAVIENTAGQGTNLGYRFEQ
IAFLIDRIEDKSRVGVCLDTCHLFASGYDLSSTEAIETTFNEFDSTVGLHYLRGMHLNDAMQPLGSRVDRHASLGKGTIG
MAAFTFIMNHPACEEIPLILETPNPDIWSEEIALLYSLQQVD

Specific function: Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by ble

COG id: COG0648

COG function: function code L; Endonuclease IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AP endonuclease 2 family

Homologues:

Organism=Escherichia coli, GI1788483, Length=279, Percent_Identity=63.4408602150538, Blast_Score=361, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17531193, Length=279, Percent_Identity=44.4444444444444, Blast_Score=273, Evalue=5e-74,
Organism=Saccharomyces cerevisiae, GI6322735, Length=280, Percent_Identity=40.3571428571429, Blast_Score=232, Evalue=6e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END4_CHLCH (Q3AT74)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378844.1
- ProteinModelPortal:   Q3AT74
- SMR:   Q3AT74
- STRING:   Q3AT74
- GeneID:   3746338
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0528
- eggNOG:   COG0648
- HOGENOM:   HBG565018
- OMA:   QIALETM
- ProtClustDB:   PRK01060
- BioCyc:   CCHL340177:CAG_0528-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00152
- InterPro:   IPR018246
- InterPro:   IPR001719
- InterPro:   IPR013022
- InterPro:   IPR012307
- Gene3D:   G3DSA:3.20.20.150
- PANTHER:   PTHR21445
- SMART:   SM00518
- TIGRFAMs:   TIGR00587

Pfam domain/function: PF01261 AP_endonuc_2; SSF51658 Xyl_isomerase-like_TIM-brl

EC number: =3.1.21.2

Molecular weight: Translated: 30794; Mature: 30794

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00729 AP_NUCLEASE_F2_1; PS00730 AP_NUCLEASE_F2_2; PS00731 AP_NUCLEASE_F2_3; PS51432 AP_NUCLEASE_F2_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRVGAHVSASGGVEQAPLNATAIGAKAFALFTKNQRQWKAPKLSKATIEAFQKACADGG
CCCCCCCEECCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
FQPQHILPHDSYLINLGSPDPEKLERARSAFIDEMQRVADLGLQLLNFHPGSHLKEISEE
CCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASLLLIAESINMALEATNGVTAVIENTAGQGTNLGYRFEQIAFLIDRIEDKSRVGVCLDT
CCEEEEEECCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
CHLFASGYDLSSTEAIETTFNEFDSTVGLHYLRGMHLNDAMQPLGSRVDRHASLGKGTIG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHH
MAAFTFIMNHPACEEIPLILETPNPDIWSEEIALLYSLQQVD
HHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRVGAHVSASGGVEQAPLNATAIGAKAFALFTKNQRQWKAPKLSKATIEAFQKACADGG
CCCCCCCEECCCCCCCCCCCHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCC
FQPQHILPHDSYLINLGSPDPEKLERARSAFIDEMQRVADLGLQLLNFHPGSHLKEISEE
CCCCEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
ASLLLIAESINMALEATNGVTAVIENTAGQGTNLGYRFEQIAFLIDRIEDKSRVGVCLDT
CCEEEEEECCCEEEECCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
CHLFASGYDLSSTEAIETTFNEFDSTVGLHYLRGMHLNDAMQPLGSRVDRHASLGKGTIG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHH
MAAFTFIMNHPACEEIPLILETPNPDIWSEEIALLYSLQQVD
HHHHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA