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The map label for this gene is purF [H]

Identifier: 78188503

GI number: 78188503

Start: 612287

End: 613780

Strand: Reverse

Name: purF [H]

Synonym: Cag_0525

Alternate gene names: 78188503

Gene position: 613780-612287 (Counterclockwise)

Preceding gene: 78188506

Following gene: 78188489

Centisome position: 23.86

GC content: 44.91

Gene sequence:

>1494_bases
ATGTGCGGAGTTTTCGGCATTTATAACTCTAAAACTCCCGCGGAAGATGCCTTTTACGGCTTATACTCGCTCCAGCATCG
CGGGCAAGAAGCGGCGGGTATTGTGGTAGCGGAGTATAACAAGGTGAAGAAAAAGACGATCTTTAAACAGCATAAAGGCA
TGGGGCTTGTGTCGGAGGTGTTTAAAGATGAATCGGTTTTTCAAAAGTTAAGCGGTTATGCGGCAATTGGTCATAATCGC
TATTCAACAACGGGTTCTTCTTCTGCTGTTAGCAACATCCAGCCTTTTTCCCTCATTTATCGCTCTGGCAGCCTTGCCAT
TGCTCACAACGGCAATCTCACCAACTCCCGCACGTTACGGCGTGAACTCACTGAGTTAGGCGTTATTTTTCAAGCCTCAT
CCGACACTGAAATTGTGCCTCATTTAGCGGCTCGTAGCCGTGAGCAAGAGCCTGTATTGCAAATTCGTGATGCGCTTTCG
CAAGTGCAGGGTGCTTACTCAATAGTATTGCTTGCCAATAATCAATTGATTGCAGCGCGTGATCCCTTTGGTTTTCGCCC
ACTTGCGCTTGGCAAAAAAGTTGATCCGTTGACGGGCGAACTTGCCTATGTGGTTGCCAGTGAAACCTGCGCTTTCGATA
TTATTCAAGCGCAATACATTCGCGATATTGAGCCGGGTGAAATTCTTTTAATTGACCATTTAGCGGTAGCCAACGAGCAA
CCGACCTCCTATTTTTTGCCTACTACCACCAACAAGGCGCGTTGCATTTTTGAGTATGTCTATTTTTCGCGCCCCGATAG
CTTTATTTTTGGGCAATCGGTGGATAAGGTGCGGCGTAACTTGGGTAAAAATCTTGCATACGAATCAACAATTCGCCAAG
AAGCGGATGAAAAAGAGTTGACGGTGGTAAGTGTGCCTGATTCTTCGAACACTGCGGCGCTTGGCTTTGTGCGTGAAAGC
ATCAAATTAGGGCGTCCTGCACGTTTTGAGCATGGTTTAATTCGCAATCACTATGTGGGTAGAACTTTTATTCAGCCCGG
TGTGCAAAGCCGCGATATTAAGGTGCGCTCAAAGTATAATATTGTGCGAGGGGTGCTGCTTAATCGTCCCATTATTTTAA
TAGATGACTCCATTGTGCGAGGTACCACAGCACGAATGCTTATTAAGCTTATTCGTGAAGCTGGTCCAAAAGCAATTCAT
TTGCACATAAGTTCACCACCTATTACCAATCCATGTTTTTATGGGATGGATTTTCCCTCAAAGCGGCAGCTTTTAACTCA
TTTATTTGAAACAAATGAGGCTGGGGAGCAAGAGATTGATAAAATTCGCGACTACATTGGGGTTGATTCGTTAAAATATC
TTTCTATGGAAGGGTTGCTCAATAGCGTGCCAAAATTTGAGGGGGAAACATGTAGCTATTGCACAGCTTGCTTTACGGGC
GTTTATCCCATTAAAGAGCATGATATGACTGCCGATAAGGATGAAAACGATTAA

Upstream 100 bases:

>100_bases
TAAAAGCATCGTTAAAAATTTCAACTTGCGCGCTTTGTGCTCTTGGTTCTTTTTAATGAATGCGGGTTCCCCTATTTATC
ATTTTATGAGAGAATAAATT

Downstream 100 bases:

>100_bases
TTGCCAAAATATATTTTTTTACACCGCTCGATAACCGCAATAAAAAAGGCGCTCTCGTTTACAAGGGCGCCTTTTTTATT
TGGATGACATATCAAAAAAG

Product: amidophosphoribosyl transferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MCGVFGIYNSKTPAEDAFYGLYSLQHRGQEAAGIVVAEYNKVKKKTIFKQHKGMGLVSEVFKDESVFQKLSGYAAIGHNR
YSTTGSSSAVSNIQPFSLIYRSGSLAIAHNGNLTNSRTLRRELTELGVIFQASSDTEIVPHLAARSREQEPVLQIRDALS
QVQGAYSIVLLANNQLIAARDPFGFRPLALGKKVDPLTGELAYVVASETCAFDIIQAQYIRDIEPGEILLIDHLAVANEQ
PTSYFLPTTTNKARCIFEYVYFSRPDSFIFGQSVDKVRRNLGKNLAYESTIRQEADEKELTVVSVPDSSNTAALGFVRES
IKLGRPARFEHGLIRNHYVGRTFIQPGVQSRDIKVRSKYNIVRGVLLNRPIILIDDSIVRGTTARMLIKLIREAGPKAIH
LHISSPPITNPCFYGMDFPSKRQLLTHLFETNEAGEQEIDKIRDYIGVDSLKYLSMEGLLNSVPKFEGETCSYCTACFTG
VYPIKEHDMTADKDEND

Sequences:

>Translated_497_residues
MCGVFGIYNSKTPAEDAFYGLYSLQHRGQEAAGIVVAEYNKVKKKTIFKQHKGMGLVSEVFKDESVFQKLSGYAAIGHNR
YSTTGSSSAVSNIQPFSLIYRSGSLAIAHNGNLTNSRTLRRELTELGVIFQASSDTEIVPHLAARSREQEPVLQIRDALS
QVQGAYSIVLLANNQLIAARDPFGFRPLALGKKVDPLTGELAYVVASETCAFDIIQAQYIRDIEPGEILLIDHLAVANEQ
PTSYFLPTTTNKARCIFEYVYFSRPDSFIFGQSVDKVRRNLGKNLAYESTIRQEADEKELTVVSVPDSSNTAALGFVRES
IKLGRPARFEHGLIRNHYVGRTFIQPGVQSRDIKVRSKYNIVRGVLLNRPIILIDDSIVRGTTARMLIKLIREAGPKAIH
LHISSPPITNPCFYGMDFPSKRQLLTHLFETNEAGEQEIDKIRDYIGVDSLKYLSMEGLLNSVPKFEGETCSYCTACFTG
VYPIKEHDMTADKDEND
>Mature_497_residues
MCGVFGIYNSKTPAEDAFYGLYSLQHRGQEAAGIVVAEYNKVKKKTIFKQHKGMGLVSEVFKDESVFQKLSGYAAIGHNR
YSTTGSSSAVSNIQPFSLIYRSGSLAIAHNGNLTNSRTLRRELTELGVIFQASSDTEIVPHLAARSREQEPVLQIRDALS
QVQGAYSIVLLANNQLIAARDPFGFRPLALGKKVDPLTGELAYVVASETCAFDIIQAQYIRDIEPGEILLIDHLAVANEQ
PTSYFLPTTTNKARCIFEYVYFSRPDSFIFGQSVDKVRRNLGKNLAYESTIRQEADEKELTVVSVPDSSNTAALGFVRES
IKLGRPARFEHGLIRNHYVGRTFIQPGVQSRDIKVRSKYNIVRGVLLNRPIILIDDSIVRGTTARMLIKLIREAGPKAIH
LHISSPPITNPCFYGMDFPSKRQLLTHLFETNEAGEQEIDKIRDYIGVDSLKYLSMEGLLNSVPKFEGETCSYCTACFTG
VYPIKEHDMTADKDEND

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=531, Percent_Identity=37.4764595103578, Blast_Score=314, Evalue=1e-85,
Organism=Homo sapiens, GI4826742, Length=188, Percent_Identity=29.2553191489362, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788651, Length=501, Percent_Identity=37.3253493013972, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1790167, Length=196, Percent_Identity=30.1020408163265, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=503, Percent_Identity=35.7852882703777, Blast_Score=298, Evalue=4e-81,
Organism=Caenorhabditis elegans, GI17532897, Length=217, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532899, Length=217, Percent_Identity=25.8064516129032, Blast_Score=70, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17539970, Length=304, Percent_Identity=25, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323958, Length=501, Percent_Identity=33.1337325349301, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24659598, Length=509, Percent_Identity=40.0785854616896, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI24659604, Length=485, Percent_Identity=40.6185567010309, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI28573187, Length=510, Percent_Identity=39.4117647058824, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI21357745, Length=212, Percent_Identity=25, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55395; Mature: 55395

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGVFGIYNSKTPAEDAFYGLYSLQHRGQEAAGIVVAEYNKVKKKTIFKQHKGMGLVSEV
CCCEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEECHHHHHHHHHHHHHCCCCHHHHH
FKDESVFQKLSGYAAIGHNRYSTTGSSSAVSNIQPFSLIYRSGSLAIAHNGNLTNSRTLR
HCCHHHHHHHCCCEEECCCCCCCCCCCHHHCCCCCEEEEEECCCEEEEECCCCCCHHHHH
RELTELGVIFQASSDTEIVPHLAARSREQEPVLQIRDALSQVQGAYSIVLLANNQLIAAR
HHHHHHCEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEC
DPFGFRPLALGKKVDPLTGELAYVVASETCAFDIIQAQYIRDIEPGEILLIDHLAVANEQ
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
PTSYFLPTTTNKARCIFEYVYFSRPDSFIFGQSVDKVRRNLGKNLAYESTIRQEADEKEL
CCEEECCCCCCCHHEEEEEHHHCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCE
TVVSVPDSSNTAALGFVRESIKLGRPARFEHGLIRNHYVGRTFIQPGVQSRDIKVRSKYN
EEEECCCCCCCCHHHHHHHHHHCCCCCCHHCCCHHHCCCCHHHHCCCCCCCCEEEHHHHH
IVRGVLLNRPIILIDDSIVRGTTARMLIKLIREAGPKAIHLHISSPPITNPCFYGMDFPS
HHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHLFETNEAGEQEIDKIRDYIGVDSLKYLSMEGLLNSVPKFEGETCSYCTACFTG
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
VYPIKEHDMTADKDEND
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MCGVFGIYNSKTPAEDAFYGLYSLQHRGQEAAGIVVAEYNKVKKKTIFKQHKGMGLVSEV
CCCEEECCCCCCCHHHHHHHHHHHHHCCCHHHEEEEECHHHHHHHHHHHHHCCCCHHHHH
FKDESVFQKLSGYAAIGHNRYSTTGSSSAVSNIQPFSLIYRSGSLAIAHNGNLTNSRTLR
HCCHHHHHHHCCCEEECCCCCCCCCCCHHHCCCCCEEEEEECCCEEEEECCCCCCHHHHH
RELTELGVIFQASSDTEIVPHLAARSREQEPVLQIRDALSQVQGAYSIVLLANNQLIAAR
HHHHHHCEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEECCEEEEEC
DPFGFRPLALGKKVDPLTGELAYVVASETCAFDIIQAQYIRDIEPGEILLIDHLAVANEQ
CCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCC
PTSYFLPTTTNKARCIFEYVYFSRPDSFIFGQSVDKVRRNLGKNLAYESTIRQEADEKEL
CCEEECCCCCCCHHEEEEEHHHCCCCCEECCCCHHHHHHHHCCCCHHHHHHHHCCCCCCE
TVVSVPDSSNTAALGFVRESIKLGRPARFEHGLIRNHYVGRTFIQPGVQSRDIKVRSKYN
EEEECCCCCCCCHHHHHHHHHHCCCCCCHHCCCHHHCCCCHHHHCCCCCCCCEEEHHHHH
IVRGVLLNRPIILIDDSIVRGTTARMLIKLIREAGPKAIHLHISSPPITNPCFYGMDFPS
HHHHHHHCCCEEEECCCHHCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCH
KRQLLTHLFETNEAGEQEIDKIRDYIGVDSLKYLSMEGLLNSVPKFEGETCSYCTACFTG
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHC
VYPIKEHDMTADKDEND
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]