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The map label for this gene is appA [H]

Identifier: 78188382

GI number: 78188382

Start: 469051

End: 470739

Strand: Reverse

Name: appA [H]

Synonym: Cag_0404

Alternate gene names: 78188382

Gene position: 470739-469051 (Counterclockwise)

Preceding gene: 78188383

Following gene: 78188380

Centisome position: 18.3

GC content: 49.62

Gene sequence:

>1689_bases
ATGGAAAAGAAACCTCCATTTGCACTCATAGTTGCATTACTGCTTGTTGTAAGCACGTTATACGGTTGCCATCAACAACG
GGCTGAAGAGCAACACCAGCAAGTAGTTACCGCCATTTCAGCCGATTTCGATTACCTCAATCCGCTGCTCATTCAGTTTG
CAATGTCGCGCGAAATGTGCAAGCTGCTCTATCCATCGCTTGTACGCCCAGCTTACGATTCAGCCCAAGGCACCATTCGC
TTCCTTCCTAACGCTGCCAAGCAATGGAAATTTTCGCCCGATGGTAAAGGTGTTACGTTCCATCTTAACCGCAATGCTCG
CTGGGAAGATGGGGTGCCTGTAACATCGCACGACTTTAAATTCTCCTACGCTCTCTACAAAAATCCAGCAGTGGCAAGTA
CGCGCCAGCATTACTTAAACGATTTACCATTGCTACCCGATGGCTCACCCGATGTTGAGCGTGCGGTGGAAACGCCGAAC
GATTCAACGTTGGTGATTCACTTTAGCAATGCTATGGCAGAGGAAATTGTGCTTGATCATCTGAATGATTTAATGCCGGT
AGCTCGCCATCGCTTTAAGGATTTTCGCCCTGAGGAGATTCGCCAACGTGCGGCTGAATTACCGCTGCTGAGTGCGGGTC
CGTTCCGCGTAAAAGAGTGGAAACGGCAAGAGCGTTTGGTGCTGGAACCAAATCCAACCTCAGCGCTACCGCATCCTGCC
ACCTTAAAAAGCATGGTCTTTATGGTGGTGCCCGAATACACCACACGCTTGGCTATGCTGAAAGCAGGGCAAATTGATGC
AATGCTTTCAGCGGGCGGCATTAACCCAAAGGATGTGCCAGAACTACACCGAAGCGCGCCCAATGTGGTGATTCGCCCTG
TACAGCACCGCTATTTTGATAGCATTGTGTGGCTCAATATTGATGGCGAATGCTATCGTACCCGCAAGCAGATTGCGCCT
CATCCGCTTTTTGGTGATAAGCGTGTTCGCCAAGCCCTTACCCTTGCCATTGATCGCCAATCCATTATTGATGGTTTTAT
GGGACCCGACCACGCCACCATTGTGAACACCTCGCTTTCGCCGGCATATCGCACCCTTGCCGATAGCTCGCTTGATGCGT
ATGCCTACAATCCACGCCGAGCTTCCATGCTCTTGCGTCAAGCAGGATGGCTACCGGGAGCAAACGGCATTCTGCAAAAA
AATGGCAAACCCTTTAGCTTTGAGCTTGCAGCACCTACCGGCAATCCACGCCGCAACTATGCCGCTACAATCATTCAGCA
AAACTTGCGGGCAATTGGCATTGATTGTCGCCTCCGCTTTGATGAAGGACTTATTTTTAACCGCAACCAAAACGAGTTTC
GCTACGATGCCGCCCTTTCGGGCATGGCAGCAGAAACCTTGCCTTTTCAGCTTATTATTTGGGGATCGAATTTTGCCGAA
CGCCCCTTTAACTCAGCCGCTTTTCAAAACAGGGAGCTTGACGAGGTGATTGCTCAACTCAGCAAACCAAATTCACCTAC
ACGAAAACGTGCCTTTTGGCAACGCTATCAGCAAATTTTGCACGAAGAACAGCCACGCACATTCCTTTATTACTATGATG
AACTTGAAGGCTTCAATAAACGCATTCGCAACGCCGAGGTAAACATGCTCTCAACGCTCTACAATCTCCACGAATGGCAA
ACGGAGTAA

Upstream 100 bases:

>100_bases
TTACCTTCGTCATTCCCGCGAAAATGATAAAAGACTTATGAATTATTCCGGTTAAGCGTTAGCACTGACGATTTTTATTC
CCCCGATGTTTTAATTGTTC

Downstream 100 bases:

>100_bases
CGGTAATTACTCATGCTTACAGGCTTTTTGTTCTTCCAACGCAGCTTCAATAAGCCGTCGTTCTTTTTCAATTTCGATTT
GTAATTGTTCTTCGCTGGGC

Product: peptide ABC transporter, periplasmic peptide-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MEKKPPFALIVALLLVVSTLYGCHQQRAEEQHQQVVTAISADFDYLNPLLIQFAMSREMCKLLYPSLVRPAYDSAQGTIR
FLPNAAKQWKFSPDGKGVTFHLNRNARWEDGVPVTSHDFKFSYALYKNPAVASTRQHYLNDLPLLPDGSPDVERAVETPN
DSTLVIHFSNAMAEEIVLDHLNDLMPVARHRFKDFRPEEIRQRAAELPLLSAGPFRVKEWKRQERLVLEPNPTSALPHPA
TLKSMVFMVVPEYTTRLAMLKAGQIDAMLSAGGINPKDVPELHRSAPNVVIRPVQHRYFDSIVWLNIDGECYRTRKQIAP
HPLFGDKRVRQALTLAIDRQSIIDGFMGPDHATIVNTSLSPAYRTLADSSLDAYAYNPRRASMLLRQAGWLPGANGILQK
NGKPFSFELAAPTGNPRRNYAATIIQQNLRAIGIDCRLRFDEGLIFNRNQNEFRYDAALSGMAAETLPFQLIIWGSNFAE
RPFNSAAFQNRELDEVIAQLSKPNSPTRKRAFWQRYQQILHEEQPRTFLYYYDELEGFNKRIRNAEVNMLSTLYNLHEWQ
TE

Sequences:

>Translated_562_residues
MEKKPPFALIVALLLVVSTLYGCHQQRAEEQHQQVVTAISADFDYLNPLLIQFAMSREMCKLLYPSLVRPAYDSAQGTIR
FLPNAAKQWKFSPDGKGVTFHLNRNARWEDGVPVTSHDFKFSYALYKNPAVASTRQHYLNDLPLLPDGSPDVERAVETPN
DSTLVIHFSNAMAEEIVLDHLNDLMPVARHRFKDFRPEEIRQRAAELPLLSAGPFRVKEWKRQERLVLEPNPTSALPHPA
TLKSMVFMVVPEYTTRLAMLKAGQIDAMLSAGGINPKDVPELHRSAPNVVIRPVQHRYFDSIVWLNIDGECYRTRKQIAP
HPLFGDKRVRQALTLAIDRQSIIDGFMGPDHATIVNTSLSPAYRTLADSSLDAYAYNPRRASMLLRQAGWLPGANGILQK
NGKPFSFELAAPTGNPRRNYAATIIQQNLRAIGIDCRLRFDEGLIFNRNQNEFRYDAALSGMAAETLPFQLIIWGSNFAE
RPFNSAAFQNRELDEVIAQLSKPNSPTRKRAFWQRYQQILHEEQPRTFLYYYDELEGFNKRIRNAEVNMLSTLYNLHEWQ
TE
>Mature_562_residues
MEKKPPFALIVALLLVVSTLYGCHQQRAEEQHQQVVTAISADFDYLNPLLIQFAMSREMCKLLYPSLVRPAYDSAQGTIR
FLPNAAKQWKFSPDGKGVTFHLNRNARWEDGVPVTSHDFKFSYALYKNPAVASTRQHYLNDLPLLPDGSPDVERAVETPN
DSTLVIHFSNAMAEEIVLDHLNDLMPVARHRFKDFRPEEIRQRAAELPLLSAGPFRVKEWKRQERLVLEPNPTSALPHPA
TLKSMVFMVVPEYTTRLAMLKAGQIDAMLSAGGINPKDVPELHRSAPNVVIRPVQHRYFDSIVWLNIDGECYRTRKQIAP
HPLFGDKRVRQALTLAIDRQSIIDGFMGPDHATIVNTSLSPAYRTLADSSLDAYAYNPRRASMLLRQAGWLPGANGILQK
NGKPFSFELAAPTGNPRRNYAATIIQQNLRAIGIDCRLRFDEGLIFNRNQNEFRYDAALSGMAAETLPFQLIIWGSNFAE
RPFNSAAFQNRELDEVIAQLSKPNSPTRKRAFWQRYQQILHEEQPRTFLYYYDELEGFNKRIRNAEVNMLSTLYNLHEWQ
TE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=523, Percent_Identity=21.9885277246654, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1789966, Length=494, Percent_Identity=20.4453441295547, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789887, Length=509, Percent_Identity=25.3438113948919, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1789397, Length=517, Percent_Identity=20.889748549323, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 64177; Mature: 64177

Theoretical pI: Translated: 8.91; Mature: 8.91

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKPPFALIVALLLVVSTLYGCHQQRAEEQHQQVVTAISADFDYLNPLLIQFAMSREMC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KLLYPSLVRPAYDSAQGTIRFLPNAAKQWKFSPDGKGVTFHLNRNARWEDGVPVTSHDFK
HHHHHHHHCCCCCCCCCEEEECCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCE
FSYALYKNPAVASTRQHYLNDLPLLPDGSPDVERAVETPNDSTLVIHFSNAMAEEIVLDH
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
LNDLMPVARHRFKDFRPEEIRQRAAELPLLSAGPFRVKEWKRQERLVLEPNPTSALPHPA
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCH
TLKSMVFMVVPEYTTRLAMLKAGQIDAMLSAGGINPKDVPELHRSAPNVVIRPVQHRYFD
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHCCCCEEEECHHHHHCC
SIVWLNIDGECYRTRKQIAPHPLFGDKRVRQALTLAIDRQSIIDGFMGPDHATIVNTSLS
EEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
PAYRTLADSSLDAYAYNPRRASMLLRQAGWLPGANGILQKNGKPFSFELAAPTGNPRRNY
HHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEECCCCCCCHHH
AATIIQQNLRAIGIDCRLRFDEGLIFNRNQNEFRYDAALSGMAAETLPFQLIIWGSNFAE
HHHHHHHHHHEEEEEEEEEECCCEEECCCCCCEEHHHHHCCCHHCCCCEEEEEECCCCCC
RPFNSAAFQNRELDEVIAQLSKPNSPTRKRAFWQRYQQILHEEQPRTFLYYYDELEGFNK
CCCCHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
RIRNAEVNMLSTLYNLHEWQTE
HHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEKKPPFALIVALLLVVSTLYGCHQQRAEEQHQQVVTAISADFDYLNPLLIQFAMSREMC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KLLYPSLVRPAYDSAQGTIRFLPNAAKQWKFSPDGKGVTFHLNRNARWEDGVPVTSHDFK
HHHHHHHHCCCCCCCCCEEEECCCHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCE
FSYALYKNPAVASTRQHYLNDLPLLPDGSPDVERAVETPNDSTLVIHFSNAMAEEIVLDH
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHHH
LNDLMPVARHRFKDFRPEEIRQRAAELPLLSAGPFRVKEWKRQERLVLEPNPTSALPHPA
HHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCH
TLKSMVFMVVPEYTTRLAMLKAGQIDAMLSAGGINPKDVPELHRSAPNVVIRPVQHRYFD
HHHHHHHHCCCHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHCCCCEEEECHHHHHCC
SIVWLNIDGECYRTRKQIAPHPLFGDKRVRQALTLAIDRQSIIDGFMGPDHATIVNTSLS
EEEEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCC
PAYRTLADSSLDAYAYNPRRASMLLRQAGWLPGANGILQKNGKPFSFELAAPTGNPRRNY
HHHHHHHCCCCCCEEECCHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEEECCCCCCCHHH
AATIIQQNLRAIGIDCRLRFDEGLIFNRNQNEFRYDAALSGMAAETLPFQLIIWGSNFAE
HHHHHHHHHHEEEEEEEEEECCCEEECCCCCCEEHHHHHCCCHHCCCCEEEEEECCCCCC
RPFNSAAFQNRELDEVIAQLSKPNSPTRKRAFWQRYQQILHEEQPRTFLYYYDELEGFNK
CCCCHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHH
RIRNAEVNMLSTLYNLHEWQTE
HHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]