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The map label for this gene is ygcM [C]

Identifier: 78188351

GI number: 78188351

Start: 429347

End: 429793

Strand: Reverse

Name: ygcM [C]

Synonym: Cag_0373

Alternate gene names: 78188351

Gene position: 429793-429347 (Counterclockwise)

Preceding gene: 78188352

Following gene: 78188350

Centisome position: 16.71

GC content: 43.18

Gene sequence:

>447_bases
ATGAACGACCTGCTTGAAAAGCCTCGCAAAATTTACGTATCGCGCAGCATTGAGTTTAACGCTGCCCATCGGCTTTGCAA
CCCCACCTTAACGGAGGAGGAGAATTGCGCCATTTACGGCAAATGCAACAATCCGCATGGACACGGGCATAACTATCTTT
TAACCATTACGCTCAGTGGAACCATTAACCGTCAAACAGGCTTTTTATTTGATTTAAAGGCACTTAAAGCAATTTTAGAA
GAAGAAATTGTAGAGCGCTTTGACCACAAGCACCTGAATTACGATGTGCCTGAATTAAGCAACTGCATTCCTACAACCGA
AATACTTGCGGTGTTAATATGGGATATTCTTGCCCAACGTTTTACAACCATTAACCCCGCAATAACCCTCCACGAAGTTC
ACCTTTATGAAACAGGAAAAAACGCCGTCCGCTATTACGGAGAATAA

Upstream 100 bases:

>100_bases
AATAGAGCACCATCGTACCGACCTTCAAGCGCTTATGCCCTCTGTAAGTGAATCGCACCAAGCGCTCATTACAAAGCATC
TCTCAACATAGTCGGAAGTT

Downstream 100 bases:

>100_bases
GCTACCGTTTTCCTCATCCAATTGCGGATGCGGCTGCCACAATGATGATGATACACTAACGCATGATGACGGATTGCTGA
CTGACAACTTAGAATCTGCC

Product: 6-pyruvoyltetrahydropterin synthase

Products: 6-pyruvoyl-5,6,7,8-tetrahydropterin; triphosphate

Alternate protein names: 6-Pyruvoyl-Tetrahydropterin Synthase; 6-Pyruvoyltetrahydropterin Synthase; 6-Pyruvoyl-Tetrahydropterin Synthase-Like Protein; 6-Pyruvoyl Tetrahydrobiopterin Synthase; 6-Pyruvoyl Tetrahydropterin Synthase Family; 6-Pyruvoyl Tetrahydropterin Synthase Family Protein; Tetrahydrobiopterin Biosynthesis-Like Protein; 6-Pyruvoyltetrahydrobiopterin Synthase; 6-Pyruvoyltetrahydropterin Synthase Synthase

Number of amino acids: Translated: 148; Mature: 148

Protein sequence:

>148_residues
MNDLLEKPRKIYVSRSIEFNAAHRLCNPTLTEEENCAIYGKCNNPHGHGHNYLLTITLSGTINRQTGFLFDLKALKAILE
EEIVERFDHKHLNYDVPELSNCIPTTEILAVLIWDILAQRFTTINPAITLHEVHLYETGKNAVRYYGE

Sequences:

>Translated_148_residues
MNDLLEKPRKIYVSRSIEFNAAHRLCNPTLTEEENCAIYGKCNNPHGHGHNYLLTITLSGTINRQTGFLFDLKALKAILE
EEIVERFDHKHLNYDVPELSNCIPTTEILAVLIWDILAQRFTTINPAITLHEVHLYETGKNAVRYYGE
>Mature_148_residues
MNDLLEKPRKIYVSRSIEFNAAHRLCNPTLTEEENCAIYGKCNNPHGHGHNYLLTITLSGTINRQTGFLFDLKALKAILE
EEIVERFDHKHLNYDVPELSNCIPTTEILAVLIWDILAQRFTTINPAITLHEVHLYETGKNAVRYYGE

Specific function: Tetrahydrobiopterin biosynthesis; second step. [C]

COG id: COG0720

COG function: function code H; 6-pyruvoyl-tetrahydropterin synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4506331, Length=136, Percent_Identity=46.3235294117647, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789124, Length=135, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115532224, Length=137, Percent_Identity=34.3065693430657, Blast_Score=90, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115532226, Length=137, Percent_Identity=34.3065693430657, Blast_Score=90, Evalue=3e-19,
Organism=Drosophila melanogaster, GI19550074, Length=136, Percent_Identity=44.8529411764706, Blast_Score=119, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24585378, Length=136, Percent_Identity=44.8529411764706, Blast_Score=119, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.3.12

Molecular weight: Translated: 17009; Mature: 17009

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00987 PTPS_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLLEKPRKIYVSRSIEFNAAHRLCNPTLTEEENCAIYGKCNNPHGHGHNYLLTITLSG
CCHHHHHHHHEEEECCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEC
TINRQTGFLFDLKALKAILEEEIVERFDHKHLNYDVPELSNCIPTTEILAVLIWDILAQR
EECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHH
FTTINPAITLHEVHLYETGKNAVRYYGE
HCCCCCEEEEEEEEEEECCCCCEEECCC
>Mature Secondary Structure
MNDLLEKPRKIYVSRSIEFNAAHRLCNPTLTEEENCAIYGKCNNPHGHGHNYLLTITLSG
CCHHHHHHHHEEEECCCCCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEEC
TINRQTGFLFDLKALKAILEEEIVERFDHKHLNYDVPELSNCIPTTEILAVLIWDILAQR
EECCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHH
FTTINPAITLHEVHLYETGKNAVRYYGE
HCCCCCEEEEEEEEEEECCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Mg2+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 17.7 {6-(L-erythro-1,2-dihydroxypropyl} 5 {6-(L-erythro-1,2-dihydroxypropyl} 0.0091 {6-(L-erythro-1,2-dihydroxypropyl} 0.008 {6-(L-erythro-1,2-dihydroxypropyl} [C]

Substrates: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin

Specific reaction: 6-[(1S,2R)-1,2-dihydroxy-3-triphosphooxypropyl]-7,8-dihydropterin = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

General reaction: Intramolecular redox reaction; P-O bond cleavage; Triphosphate elimination [C]

Inhibitor: (NH4)2SO4 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA