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The map label for this gene is folE

Identifier: 78188350

GI number: 78188350

Start: 428716

End: 429387

Strand: Reverse

Name: folE

Synonym: Cag_0372

Alternate gene names: 78188350

Gene position: 429387-428716 (Counterclockwise)

Preceding gene: 78188351

Following gene: 78188346

Centisome position: 16.69

GC content: 47.47

Gene sequence:

>672_bases
ATGAAACAGGAAAAAACGCCGTCCGCTATTACGGAGAATAAGCTACCGTTTTCCTCATCCAATTGCGGATGCGGCTGCCA
CAATGATGATGATACACTAACGCATGATGACGGATTGCTGACTGACAACTTAGAATCTGCCGTTTACACCATGCTGCAAA
ACGTGGGTGAAGATCCCCAGCGTGAAGGGTTGCTAAAAACCCCTGAGCGCGTTGCACGCTCCATGCGCTTTCTTACCAAA
GGGTATCACGAAAACCCCGAAGAGCTGTTACAAAAAGCCCTCTTTACCGAAAGCTATGACGAAATGGTATTAGTGCGCGA
TATTGATCTTTTTTCGATGTGCGAGCACCACATGCTTCCCTTTTTTGGCAAAGCCCACGTAGCATACATTCCCGATGGCA
AAATTGTAGGACTTTCAAAGCTTGCCCGTGTGGTTGAAGTTTTTTCGCGCCGACTACAAGTGCAAGAGCGCCTAACGCAG
CAAATTCGTGATGCAATTCAAAACGTGCTGAATCCCAAAGGCGTTGCTGTGGTTATTGAGGCAAAGCACCTTTGCATGGT
AATGCGTGGCGTTGAAAAGCTCAATTCCATTACCACAACCTCCGCGATGTCGGGCGTTTTTATGACCTCACCGTCAACAC
GCGGCGAGTTTTTGCGCTTAATCCAAAAATAA

Upstream 100 bases:

>100_bases
ATTCCTACAACCGAAATACTTGCGGTGTTAATATGGGATATTCTTGCCCAACGTTTTACAACCATTAACCCCGCAATAAC
CCTCCACGAAGTTCACCTTT

Downstream 100 bases:

>100_bases
AAAATGGATGGTGGAAAGAGCTGCAACACCAACGGCTCTTTCCTCTCTCTTTGTTGCACCTCAACATGATATGCTTGGTA
AGCACAAAAGAAAAAGACAA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MKQEKTPSAITENKLPFSSSNCGCGCHNDDDTLTHDDGLLTDNLESAVYTMLQNVGEDPQREGLLKTPERVARSMRFLTK
GYHENPEELLQKALFTESYDEMVLVRDIDLFSMCEHHMLPFFGKAHVAYIPDGKIVGLSKLARVVEVFSRRLQVQERLTQ
QIRDAIQNVLNPKGVAVVIEAKHLCMVMRGVEKLNSITTTSAMSGVFMTSPSTRGEFLRLIQK

Sequences:

>Translated_223_residues
MKQEKTPSAITENKLPFSSSNCGCGCHNDDDTLTHDDGLLTDNLESAVYTMLQNVGEDPQREGLLKTPERVARSMRFLTK
GYHENPEELLQKALFTESYDEMVLVRDIDLFSMCEHHMLPFFGKAHVAYIPDGKIVGLSKLARVVEVFSRRLQVQERLTQ
QIRDAIQNVLNPKGVAVVIEAKHLCMVMRGVEKLNSITTTSAMSGVFMTSPSTRGEFLRLIQK
>Mature_223_residues
MKQEKTPSAITENKLPFSSSNCGCGCHNDDDTLTHDDGLLTDNLESAVYTMLQNVGEDPQREGLLKTPERVARSMRFLTK
GYHENPEELLQKALFTESYDEMVLVRDIDLFSMCEHHMLPFFGKAHVAYIPDGKIVGLSKLARVVEVFSRRLQVQERLTQ
QIRDAIQNVLNPKGVAVVIEAKHLCMVMRGVEKLNSITTTSAMSGVFMTSPSTRGEFLRLIQK

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family

Homologues:

Organism=Homo sapiens, GI66932968, Length=204, Percent_Identity=54.9019607843137, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI4503949, Length=204, Percent_Identity=54.9019607843137, Blast_Score=241, Evalue=5e-64,
Organism=Homo sapiens, GI66932970, Length=178, Percent_Identity=50, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI66932972, Length=163, Percent_Identity=52.760736196319, Blast_Score=189, Evalue=2e-48,
Organism=Escherichia coli, GI1788476, Length=176, Percent_Identity=43.1818181818182, Blast_Score=126, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560486, Length=218, Percent_Identity=55.045871559633, Blast_Score=243, Evalue=8e-65,
Organism=Saccharomyces cerevisiae, GI6321706, Length=181, Percent_Identity=53.5911602209945, Blast_Score=208, Evalue=7e-55,
Organism=Drosophila melanogaster, GI24656796, Length=214, Percent_Identity=51.8691588785047, Blast_Score=213, Evalue=6e-56,
Organism=Drosophila melanogaster, GI24656782, Length=213, Percent_Identity=52.112676056338, Blast_Score=213, Evalue=7e-56,
Organism=Drosophila melanogaster, GI24656791, Length=225, Percent_Identity=51.1111111111111, Blast_Score=213, Evalue=8e-56,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): GCH1_CHLCH (Q3ATN0)

Other databases:

- EMBL:   CP000108
- RefSeq:   YP_378688.1
- ProteinModelPortal:   Q3ATN0
- SMR:   Q3ATN0
- STRING:   Q3ATN0
- GeneID:   3748017
- GenomeReviews:   CP000108_GR
- KEGG:   cch:Cag_0372
- eggNOG:   COG0302
- HOGENOM:   HBG370191
- OMA:   YALEPRE
- ProtClustDB:   PRK09347
- BioCyc:   CCHL340177:CAG_0372-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00223
- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234
- TIGRFAMs:   TIGR00063

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =3.5.4.16

Molecular weight: Translated: 25103; Mature: 25103

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEKTPSAITENKLPFSSSNCGCGCHNDDDTLTHDDGLLTDNLESAVYTMLQNVGEDPQ
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCHH
REGLLKTPERVARSMRFLTKGYHENPEELLQKALFTESYDEMVLVRDIDLFSMCEHHMLP
HCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCC
FFGKAHVAYIPDGKIVGLSKLARVVEVFSRRLQVQERLTQQIRDAIQNVLNPKGVAVVIE
CCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEH
AKHLCMVMRGVEKLNSITTTSAMSGVFMTSPSTRGEFLRLIQK
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCC
>Mature Secondary Structure
MKQEKTPSAITENKLPFSSSNCGCGCHNDDDTLTHDDGLLTDNLESAVYTMLQNVGEDPQ
CCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHCCCHH
REGLLKTPERVARSMRFLTKGYHENPEELLQKALFTESYDEMVLVRDIDLFSMCEHHMLP
HCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHCCC
FFGKAHVAYIPDGKIVGLSKLARVVEVFSRRLQVQERLTQQIRDAIQNVLNPKGVAVVIE
CCCCCEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEH
AKHLCMVMRGVEKLNSITTTSAMSGVFMTSPSTRGEFLRLIQK
HHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA