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The map label for this gene is cmcAX [H]

Identifier: 78188317

GI number: 78188317

Start: 381437

End: 382633

Strand: Reverse

Name: cmcAX [H]

Synonym: Cag_0339

Alternate gene names: 78188317

Gene position: 382633-381437 (Counterclockwise)

Preceding gene: 78188318

Following gene: 78188316

Centisome position: 14.88

GC content: 42.86

Gene sequence:

>1197_bases
ATGAGGCGTTTTTGGACTTTTTTGTTGATTAGTTGTGCTCCTTTCTTTTTATCGAGTTGCTCATCTGTTCCACAAAATCC
AGAAAAGATTTCTACGGAAGCATGGCACTACTATCGCGATACGTTTATAAAAAATGGTCGCGTTATTCGTCCTCAAAACA
ATAATGATACTGTTTCGGAGGGGCAAGCTTACACAATGGTTCGTGCGGTTTTAATGAAGGATCGCAAAACCTTTGATGAA
TGCCTTGCTTGGAGCGAAAAGGTGCTCTCCCGTAAAAATAGTGATGGTGATTATTTGCTTGCATGGCATTACCGTGATGG
CAAAGTTACCGATACAACGGCAGCCTCTGATGCCGATATTGATTATGCTTTTAGCTTAATTGTTGCCTCAAAAATATGGC
AGGCACCTCGTTACCTTGAGCTTGCTAAAGAGGTGCTTGCAAGCATTCTGGAAGCGGAAACCACGCGCCATCAAGGGCGT
TTATATTTATTGCCGTGGCCTGCAAACAAAAACAAGCCAGGTGATTTATTAGCACAAAACCTCTCTTACTACGCACCTTC
CCATTTCAAGCTTTTTTACGAAACCACAAGCGACCCTCGTTGGCTTGAGCTTGTGGATACAACCTACTACTTATTGGGAC
GCTTGCTGCACCCTGGTGAATTACCAGAGGGACCTATTGTGCCTGATTGGATTGCTATAAATGATGCTGGCGCTTTTGTG
CATTTACCGGGTAAAGATGTACGCTATGGCTGGGATGCTGTAAGAGTGCCAATGAGAATTGCGGCTGATTACCATTTATA
TGGCGATAAACGTGCTTTTGAGGTGCTCAGTTGGTTAGCGGTATCGTTTGAGGAGGAATTTCGCCAACAATCAAAATTTT
TGTTACAGCGAGATTCAACCCTCCAAGTGCGTAATAATGCCCTTTTTTATAGCGCTATGTATGCTTCGTTAGAAGCAACG
GAATCGCCAAGTGCCCCTAAGTTGCTACAACGCATTCGCAAGTTTATTAGGCAAGAGAAGCAAGGTTTGTTTTATAATCA
TCCTGATGATTACTACATCAACAGCCTATGCTGGATTACGGAGTATTACGAGCAAAACAAAAAACACTTACAAGCACGTT
CTAAAAAAGTTGCTCTTCCATTACAAACCCACGAAAGTACAGCAAACACGGCATCCTTGAGTTTACAAGCACCATAG

Upstream 100 bases:

>100_bases
GAATAGTCTTCTTTGAATTTGATTACATGTTGGTAATAAATGTTATTTGGACAACTTTGCAAGCCTTTATGCTTTCGGGG
CTCTTTTATTTCAATAAAAA

Downstream 100 bases:

>100_bases
CTTATGAAGATTGCATAAAAGCTATTTTCCAATAACGGTATTTTCATTATACTCCCCCTCCTTCCGGCGATACGCCATCC
TATTAAGGCATCACTAAAAT

Product: endoglucanase Y-like protein

Products: NA

Alternate protein names: Cellulase; Endo-1,4-beta-glucanase [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MRRFWTFLLISCAPFFLSSCSSVPQNPEKISTEAWHYYRDTFIKNGRVIRPQNNNDTVSEGQAYTMVRAVLMKDRKTFDE
CLAWSEKVLSRKNSDGDYLLAWHYRDGKVTDTTAASDADIDYAFSLIVASKIWQAPRYLELAKEVLASILEAETTRHQGR
LYLLPWPANKNKPGDLLAQNLSYYAPSHFKLFYETTSDPRWLELVDTTYYLLGRLLHPGELPEGPIVPDWIAINDAGAFV
HLPGKDVRYGWDAVRVPMRIAADYHLYGDKRAFEVLSWLAVSFEEEFRQQSKFLLQRDSTLQVRNNALFYSAMYASLEAT
ESPSAPKLLQRIRKFIRQEKQGLFYNHPDDYYINSLCWITEYYEQNKKHLQARSKKVALPLQTHESTANTASLSLQAP

Sequences:

>Translated_398_residues
MRRFWTFLLISCAPFFLSSCSSVPQNPEKISTEAWHYYRDTFIKNGRVIRPQNNNDTVSEGQAYTMVRAVLMKDRKTFDE
CLAWSEKVLSRKNSDGDYLLAWHYRDGKVTDTTAASDADIDYAFSLIVASKIWQAPRYLELAKEVLASILEAETTRHQGR
LYLLPWPANKNKPGDLLAQNLSYYAPSHFKLFYETTSDPRWLELVDTTYYLLGRLLHPGELPEGPIVPDWIAINDAGAFV
HLPGKDVRYGWDAVRVPMRIAADYHLYGDKRAFEVLSWLAVSFEEEFRQQSKFLLQRDSTLQVRNNALFYSAMYASLEAT
ESPSAPKLLQRIRKFIRQEKQGLFYNHPDDYYINSLCWITEYYEQNKKHLQARSKKVALPLQTHESTANTASLSLQAP
>Mature_398_residues
MRRFWTFLLISCAPFFLSSCSSVPQNPEKISTEAWHYYRDTFIKNGRVIRPQNNNDTVSEGQAYTMVRAVLMKDRKTFDE
CLAWSEKVLSRKNSDGDYLLAWHYRDGKVTDTTAASDADIDYAFSLIVASKIWQAPRYLELAKEVLASILEAETTRHQGR
LYLLPWPANKNKPGDLLAQNLSYYAPSHFKLFYETTSDPRWLELVDTTYYLLGRLLHPGELPEGPIVPDWIAINDAGAFV
HLPGKDVRYGWDAVRVPMRIAADYHLYGDKRAFEVLSWLAVSFEEEFRQQSKFLLQRDSTLQVRNNALFYSAMYASLEAT
ESPSAPKLLQRIRKFIRQEKQGLFYNHPDDYYINSLCWITEYYEQNKKHLQARSKKVALPLQTHESTANTASLSLQAP

Specific function: Enzyme capable of hydrolyzing carboxy-methyl-cellulose (CMC) [H]

COG id: COG3405

COG function: function code G; Endoglucanase Y

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family [H]

Homologues:

Organism=Escherichia coli, GI1789951, Length=241, Percent_Identity=27.8008298755187, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008928
- InterPro:   IPR012341
- InterPro:   IPR002037
- InterPro:   IPR019834 [H]

Pfam domain/function: PF01270 Glyco_hydro_8 [H]

EC number: =3.2.1.4 [H]

Molecular weight: Translated: 46026; Mature: 46026

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRFWTFLLISCAPFFLSSCSSVPQNPEKISTEAWHYYRDTFIKNGRVIRPQNNNDTVSE
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHCCCEEECCCCCCCCCCC
GQAYTMVRAVLMKDRKTFDECLAWSEKVLSRKNSDGDYLLAWHYRDGKVTDTTAASDADI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCCH
DYAFSLIVASKIWQAPRYLELAKEVLASILEAETTRHQGRLYLLPWPANKNKPGDLLAQN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
LSYYAPSHFKLFYETTSDPRWLELVDTTYYLLGRLLHPGELPEGPIVPDWIAINDAGAFV
HHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEE
HLPGKDVRYGWDAVRVPMRIAADYHLYGDKRAFEVLSWLAVSFEEEFRQQSKFLLQRDST
ECCCCCCCCCCHHHCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LQVRNNALFYSAMYASLEATESPSAPKLLQRIRKFIRQEKQGLFYNHPDDYYINSLCWIT
EEEECCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EYYEQNKKHLQARSKKVALPLQTHESTANTASLSLQAP
HHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECC
>Mature Secondary Structure
MRRFWTFLLISCAPFFLSSCSSVPQNPEKISTEAWHYYRDTFIKNGRVIRPQNNNDTVSE
CCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCHHHHHHHHHHHHCCCEEECCCCCCCCCCC
GQAYTMVRAVLMKDRKTFDECLAWSEKVLSRKNSDGDYLLAWHYRDGKVTDTTAASDADI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCEECCCCCCCCCH
DYAFSLIVASKIWQAPRYLELAKEVLASILEAETTRHQGRLYLLPWPANKNKPGDLLAQN
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHH
LSYYAPSHFKLFYETTSDPRWLELVDTTYYLLGRLLHPGELPEGPIVPDWIAINDAGAFV
HHHCCCCCEEEEEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCEE
HLPGKDVRYGWDAVRVPMRIAADYHLYGDKRAFEVLSWLAVSFEEEFRQQSKFLLQRDST
ECCCCCCCCCCHHHCCCHHEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LQVRNNALFYSAMYASLEATESPSAPKLLQRIRKFIRQEKQGLFYNHPDDYYINSLCWIT
EEEECCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHH
EYYEQNKKHLQARSKKVALPLQTHESTANTASLSLQAP
HHHHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8300521 [H]