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The map label for this gene is rsmD [H]

Identifier: 78067607

GI number: 78067607

Start: 3280931

End: 3281545

Strand: Reverse

Name: rsmD [H]

Synonym: Bcep18194_A6138

Alternate gene names: 78067607

Gene position: 3281545-3280931 (Counterclockwise)

Preceding gene: 78067609

Following gene: 78067606

Centisome position: 88.83

GC content: 71.38

Gene sequence:

>615_bases
ATGTCCCGTTCCTCTCCCGGCCGCCCGGCGGCCCCTTCCGGCCGCGGCAAGCCGCACACGATCCGCATCATCGGCGGTGA
CTGGAAACGCACGCCGCTCGCGGTGCTCGATCTCGATGGCCTGCGGCCGACGCCCGATCGCGTGCGCGAGACGCTGTTCA
ACTGGCTCGGCCAGGATCTCGAAGGCTGGCGCTGCCTCGACCTGTTCGCAGGCACCGGCGCGCTCGGCTTCGAGGCCGCG
TCGCGCGGCGCGGCAAGCGTCGTGATGGTCGAGCGCCATCCGCGTGCCGCGCAGCAGCTGCGTGCAATCAAGGACAAGCT
CGGCGCGCGCGCGGTCGAAGTCGCGGAGGCGGACGCGTTGCGGCTCGCGGCCGGGCTCACGCCGGGCGCGTTCGACGTCG
TGTTTCTCGATCCGCCGTTCGACGAGCTGGCCGTCCTCGAGCGCGCGATTGCGCTGGCGGCGCCGCTCGTCGCGGCAGGC
GGCGCGCTGTACGTCGAGACGGGGGCGGAGCTCGATCCGGCCGCGCACGAAGCGCTCGCCGGCTGGGAGGTCGTGAAGCA
CGGCAAGGCCGGTGCGGTTCACTATCATTTGCTGCGGCGCGAAAATGATGAATAA

Upstream 100 bases:

>100_bases
CGGGGCGCTCGCGTCAGTGTGCGCCGCCGCTGTGGTAACGTGCGCCTGTTTAGCGGTGCGCCCCCGCGCGCGCCCGGTCC
CTCCGTCGTCGATATTCCGT

Downstream 100 bases:

>100_bases
TGCGCGTTCCATACGAGGCGGCGCGCCGCAAAGGCGACGCATGCCCATCTCGGCAGCGGCGTGCGCATCGCACGGCTGCC
ATCCCGTTTGCGTGATGACA

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m2G966 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase [H]

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MSRSSPGRPAAPSGRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDRVRETLFNWLGQDLEGWRCLDLFAGTGALGFEAA
SRGAASVVMVERHPRAAQQLRAIKDKLGARAVEVAEADALRLAAGLTPGAFDVVFLDPPFDELAVLERAIALAAPLVAAG
GALYVETGAELDPAAHEALAGWEVVKHGKAGAVHYHLLRRENDE

Sequences:

>Translated_204_residues
MSRSSPGRPAAPSGRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDRVRETLFNWLGQDLEGWRCLDLFAGTGALGFEAA
SRGAASVVMVERHPRAAQQLRAIKDKLGARAVEVAEADALRLAAGLTPGAFDVVFLDPPFDELAVLERAIALAAPLVAAG
GALYVETGAELDPAAHEALAGWEVVKHGKAGAVHYHLLRRENDE
>Mature_203_residues
SRSSPGRPAAPSGRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDRVRETLFNWLGQDLEGWRCLDLFAGTGALGFEAAS
RGAASVVMVERHPRAAQQLRAIKDKLGARAVEVAEADALRLAAGLTPGAFDVVFLDPPFDELAVLERAIALAAPLVAAGG
ALYVETGAELDPAAHEALAGWEVVKHGKAGAVHYHLLRRENDE

Specific function: Specifically methylates the guanosine in position 966 of 16S rRNA in the assembled 30S particle [H]

COG id: COG0742

COG function: function code L; N6-adenine-specific methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmD family [H]

Homologues:

Organism=Escherichia coli, GI1789875, Length=192, Percent_Identity=42.1875, Blast_Score=115, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004398 [H]

Pfam domain/function: PF03602 Cons_hypoth95 [H]

EC number: =2.1.1.171 [H]

Molecular weight: Translated: 21718; Mature: 21587

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRSSPGRPAAPSGRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDRVRETLFNWLGQDL
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
EGWRCLDLFAGTGALGFEAASRGAASVVMVERHPRAAQQLRAIKDKLGARAVEVAEADAL
CCCEEEEEHHCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
RLAAGLTPGAFDVVFLDPPFDELAVLERAIALAAPLVAAGGALYVETGAELDPAAHEALA
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
GWEVVKHGKAGAVHYHLLRRENDE
HHHHHHCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SRSSPGRPAAPSGRGKPHTIRIIGGDWKRTPLAVLDLDGLRPTPDRVRETLFNWLGQDL
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCC
EGWRCLDLFAGTGALGFEAASRGAASVVMVERHPRAAQQLRAIKDKLGARAVEVAEADAL
CCCEEEEEHHCCCCCCHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHH
RLAAGLTPGAFDVVFLDPPFDELAVLERAIALAAPLVAAGGALYVETGAELDPAAHEALA
HHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHH
GWEVVKHGKAGAVHYHLLRRENDE
HHHHHHCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]