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The map label for this gene is bah [H]

Identifier: 78067544

GI number: 78067544

Start: 3217601

End: 3218563

Strand: Reverse

Name: bah [H]

Synonym: Bcep18194_A6075

Alternate gene names: 78067544

Gene position: 3218563-3217601 (Counterclockwise)

Preceding gene: 78067545

Following gene: 78067542

Centisome position: 87.13

GC content: 72.79

Gene sequence:

>963_bases
ATGAGTTGGCAAAGCAAGTTCGCCTGCTGGCTGCTGCGCTGGCAGTTCCGGCCCGAGACCACGCGCGAGGTGCTGGATCC
TGCCCGCGCACGGCGCTTTACCGACCTGCGGATGGTCGTGCCGCGCCGTGCGCCGTCGGGCTACCGGCTGCGCCAGTGCT
ATGGCCCGGGCGACGCGCCGCTGCGCGGCGAGTGGCTCGAACGCACCGACGCGGGCGCGGGCCGCGGGCCCGGCCGCACG
CTGCTGTACTTCCACGGCGGCGGCTATTACTTCTGTTCGACGAAGACCCACCGTCCGCTCGTGTTCGGCCTGACGAAGCG
CGCGGGCGTGCGCTCGTTCTCGCTCGACTACCGGCTGGCGCCCGAAAACCGCTTCCCGGCCGCGCTCGACGATGCGCTGG
CCGCGTACCGGCAACTGCTGGCGCTCGGCACGCCGCCCGAGTCGATCGTGCTCGGCGGCGATTCGGCGGGCGGCGGCCTT
GCGCTTGCGACGCTCGTTGCGCTGCGCGACCGCGGCGAGCCGCTGCCGGCCGGCGCGATCCTGTTCTCGCCGTGGACCGA
CCTGGCCGCCACCGGCGCCACGCTGCGCACGAACGACGGCGCCGATCCGATGTTCGCGGGTGCGGCGCTGCCGAAGGCCG
CGAAGCTGTACCTGGGCGACGAGCCCGGCACGAATCCGTACGCATCGCCGCTCTATGCGGATTTCGCGGGCCTGCCGCCG
CTGTATATCCAGGTCGGCAGCACCGAGGTGCTGCTCGACGATTCGCGCCGCGTCGCCGAGAAGGCAAAGGCGGCCGGGGT
AGCGGTGGAGATCGAGGTGTGGCCGGACATGCCGCACGTGTGGCAGCTGTACGCGCCGATGGTGCCCGAAGCGCGCGACG
CGCTCGACCGCGCGGCCGCGTTCCTGCGCCGCGTCGCGGTCGAACGTGCGGTTCAGCGGGCCGGCGAGGCGTCGATCGCC
TGA

Upstream 100 bases:

>100_bases
AGCCTTCAGGCGCGGGAGGATGTCAAGTAGAGGCACTTGTCGAGCGGTGCGGGAACGTTCGCACGTACCATCGTCACACT
GTGATTACGGAGTGCGTCCG

Downstream 100 bases:

>100_bases
TAGGCGGCCTTGACGGCCACCGAGCCATACTTGCGCTCGAGCCGGCGCACGGTGAAGTGGCCGTGCGCCATCTGCTGGAA
GTGGTCGATGAACAGCGTAT

Product: lipolytic protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 319

Protein sequence:

>320_residues
MSWQSKFACWLLRWQFRPETTREVLDPARARRFTDLRMVVPRRAPSGYRLRQCYGPGDAPLRGEWLERTDAGAGRGPGRT
LLYFHGGGYYFCSTKTHRPLVFGLTKRAGVRSFSLDYRLAPENRFPAALDDALAAYRQLLALGTPPESIVLGGDSAGGGL
ALATLVALRDRGEPLPAGAILFSPWTDLAATGATLRTNDGADPMFAGAALPKAAKLYLGDEPGTNPYASPLYADFAGLPP
LYIQVGSTEVLLDDSRRVAEKAKAAGVAVEIEVWPDMPHVWQLYAPMVPEARDALDRAAAFLRRVAVERAVQRAGEASIA

Sequences:

>Translated_320_residues
MSWQSKFACWLLRWQFRPETTREVLDPARARRFTDLRMVVPRRAPSGYRLRQCYGPGDAPLRGEWLERTDAGAGRGPGRT
LLYFHGGGYYFCSTKTHRPLVFGLTKRAGVRSFSLDYRLAPENRFPAALDDALAAYRQLLALGTPPESIVLGGDSAGGGL
ALATLVALRDRGEPLPAGAILFSPWTDLAATGATLRTNDGADPMFAGAALPKAAKLYLGDEPGTNPYASPLYADFAGLPP
LYIQVGSTEVLLDDSRRVAEKAKAAGVAVEIEVWPDMPHVWQLYAPMVPEARDALDRAAAFLRRVAVERAVQRAGEASIA
>Mature_319_residues
SWQSKFACWLLRWQFRPETTREVLDPARARRFTDLRMVVPRRAPSGYRLRQCYGPGDAPLRGEWLERTDAGAGRGPGRTL
LYFHGGGYYFCSTKTHRPLVFGLTKRAGVRSFSLDYRLAPENRFPAALDDALAAYRQLLALGTPPESIVLGGDSAGGGLA
LATLVALRDRGEPLPAGAILFSPWTDLAATGATLRTNDGADPMFAGAALPKAAKLYLGDEPGTNPYASPLYADFAGLPPL
YIQVGSTEVLLDDSRRVAEKAKAAGVAVEIEVWPDMPHVWQLYAPMVPEARDALDRAAAFLRRVAVERAVQRAGEASIA

Specific function: This protein removes the N-acetyl group from bialaphos as one of the final steps of the bialaphos biosynthetic pathway [H]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI21328446, Length=143, Percent_Identity=32.1678321678322, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786682, Length=236, Percent_Identity=29.6610169491525, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567059, Length=259, Percent_Identity=28.5714285714286, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI115533412, Length=137, Percent_Identity=32.8467153284672, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115533410, Length=137, Percent_Identity=32.8467153284672, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 34800; Mature: 34669

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQSKFACWLLRWQFRPETTREVLDPARARRFTDLRMVVPRRAPSGYRLRQCYGPGDAP
CCCCHHEEEEEEEEECCCCHHHHHHCHHHHHHHHHHEEEECCCCCCCCEEEEECCCCCCC
LRGEWLERTDAGAGRGPGRTLLYFHGGGYYFCSTKTHRPLVFGLTKRAGVRSFSLDYRLA
CCCHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCCCCEEEEECCCCCCEEEEEEEEEC
PENRFPAALDDALAAYRQLLALGTPPESIVLGGDSAGGGLALATLVALRDRGEPLPAGAI
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEE
LFSPWTDLAATGATLRTNDGADPMFAGAALPKAAKLYLGDEPGTNPYASPLYADFAGLPP
EECCCHHHHHCCCEEECCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCE
LYIQVGSTEVLLDDSRRVAEKAKAAGVAVEIEVWPDMPHVWQLYAPMVPEARDALDRAAA
EEEEECCEEEEECCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHH
FLRRVAVERAVQRAGEASIA
HHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SWQSKFACWLLRWQFRPETTREVLDPARARRFTDLRMVVPRRAPSGYRLRQCYGPGDAP
CCCHHEEEEEEEEECCCCHHHHHHCHHHHHHHHHHEEEECCCCCCCCEEEEECCCCCCC
LRGEWLERTDAGAGRGPGRTLLYFHGGGYYFCSTKTHRPLVFGLTKRAGVRSFSLDYRLA
CCCHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCCCCEEEEECCCCCCEEEEEEEEEC
PENRFPAALDDALAAYRQLLALGTPPESIVLGGDSAGGGLALATLVALRDRGEPLPAGAI
CCCCCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEE
LFSPWTDLAATGATLRTNDGADPMFAGAALPKAAKLYLGDEPGTNPYASPLYADFAGLPP
EECCCHHHHHCCCEEECCCCCCCCHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHCCCCE
LYIQVGSTEVLLDDSRRVAEKAKAAGVAVEIEVWPDMPHVWQLYAPMVPEARDALDRAAA
EEEEECCEEEEECCCHHHHHHHHHCCEEEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHH
FLRRVAVERAVQRAGEASIA
HHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2066341 [H]