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The map label for this gene is thcD [H]

Identifier: 78067477

GI number: 78067477

Start: 3142741

End: 3143991

Strand: Reverse

Name: thcD [H]

Synonym: Bcep18194_A6008

Alternate gene names: 78067477

Gene position: 3143991-3142741 (Counterclockwise)

Preceding gene: 78067478

Following gene: 78067473

Centisome position: 85.11

GC content: 73.14

Gene sequence:

>1251_bases
ATGACGAACGACCGGGTGATGGCGATCGTCGGCGCGGGCCACGCGGGCGGCCGCGCGGCGCAGGTGCTGCGTGAGTCCGG
CTGGGGCGGGCGGATCGTGCTGATCGGCGCGGAAGCGCACCTGCCGTACGAACGGCCGCCGTTGTCGAAGGGCGTGCTGA
CCGGCGAACGCAGCGCGGCGCGGTGCCGGTTGCGCGAGCCGGAGGCCTGGGTGGCCGATCGCATCGAGCCGGTCGTCGCG
ACGGTCGAGCGTATCGATCCGTGCGCGCGGGAAGTGCACGTGTCGGGCGCTCGCGTGTTCGGCTACGACGCGCTGCTGCT
CGCGACCGGCGGCCGCGCGCGTCGACTCGCGATTCCGGGCGCGACGCTCGACGGCGTGTTTGCGTTGCGCACGCTCGACG
ACGCGGCGGCGCTCGGCTCGCGGCTCGTGCCCGATGCGCGGGTCGTGCTGATCGGCGGCGGCTTCATCGGTCTCGAAGTC
GCGGCATCGGCGCGACGGCGCGGCTGCCGCGTGACGGTGCTCGATGCGGCGCCGCGCCTGCTCGGGCGCGCGGTGCCGGA
GACGATCGCGGCGCGCGTGCACGCGCTTCACGAACAGCAGGGCGTGGCCATTCGCGTGAGCCGGAAGCCGGTGGCGATCG
AGCGGACGGCGAGCGGCGCGTTGGCCGTCGTGCTCGACGACGGCGACACGCTGATCGCCGACACGGTCGTCGCCGGCATC
GGCATCGAGCCGGCCGACGAGCTGGCGCGCGATGCGGGCCTCGCGGTCGAGCGTGGCATCGTCGTGAACGAGCGGCTCGA
GACGTCGGCGCACGGCATTTACGCGGCCGGCGACGTCGCGGTGTTTCCGAGTGCGTTGTCGGGGCGGCCGGTCCGGCAGG
AGACGTGGCACGGCGCCGAGACCCAGGCGCGCGTCGCCGCGCGCAACATGCTCGGCGCCGACGAGCCGTATCGCGACACG
CCGTGGTTCTGGTCGGATCAATACGACGCGCAGCTGCACGTGGCCGGGGAGCCGGCGCTCGGCGAGCGGTCGGTCGCGCG
CGTGCTCGGCGACGACGCGGAAATCCATTTCCATTTCGACGCCGGTGCGCGGCTCGTTGCCGCGAGTGGGTTCGGCCGTG
CGTCGGGATTCATCAAGGAAATGCGGGTTGCGCGGATGCTGGTCGAGCGTGGGGCGGGCGTGACGCCGACCGCAGTGGCG
GACGTGAACGTGAAGCTGAAATCGCTGATCGGAGAGGCGGGCGGCAGGTAG

Upstream 100 bases:

>100_bases
GTGCGTGACGGCCGGCCGCTGTCCGCGCCCGCCTGCGAGAAGCTGCGCACGTACCGCGCGAAGATCGAAGACGGTCGCAT
CCTGATCGAGGTGTGACGCG

Downstream 100 bases:

>100_bases
CGAAGCGAGGCGCGCAATGCGCATCTGGCGCGAGCCACGGGCCGTGGGAACGAGTACGAAGGAGGGGAGAAGCGGCACGG
ATGCGATACCCGCGCCGCTA

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 416; Mature: 415

Protein sequence:

>416_residues
MTNDRVMAIVGAGHAGGRAAQVLRESGWGGRIVLIGAEAHLPYERPPLSKGVLTGERSAARCRLREPEAWVADRIEPVVA
TVERIDPCAREVHVSGARVFGYDALLLATGGRARRLAIPGATLDGVFALRTLDDAAALGSRLVPDARVVLIGGGFIGLEV
AASARRRGCRVTVLDAAPRLLGRAVPETIAARVHALHEQQGVAIRVSRKPVAIERTASGALAVVLDDGDTLIADTVVAGI
GIEPADELARDAGLAVERGIVVNERLETSAHGIYAAGDVAVFPSALSGRPVRQETWHGAETQARVAARNMLGADEPYRDT
PWFWSDQYDAQLHVAGEPALGERSVARVLGDDAEIHFHFDAGARLVAASGFGRASGFIKEMRVARMLVERGAGVTPTAVA
DVNVKLKSLIGEAGGR

Sequences:

>Translated_416_residues
MTNDRVMAIVGAGHAGGRAAQVLRESGWGGRIVLIGAEAHLPYERPPLSKGVLTGERSAARCRLREPEAWVADRIEPVVA
TVERIDPCAREVHVSGARVFGYDALLLATGGRARRLAIPGATLDGVFALRTLDDAAALGSRLVPDARVVLIGGGFIGLEV
AASARRRGCRVTVLDAAPRLLGRAVPETIAARVHALHEQQGVAIRVSRKPVAIERTASGALAVVLDDGDTLIADTVVAGI
GIEPADELARDAGLAVERGIVVNERLETSAHGIYAAGDVAVFPSALSGRPVRQETWHGAETQARVAARNMLGADEPYRDT
PWFWSDQYDAQLHVAGEPALGERSVARVLGDDAEIHFHFDAGARLVAASGFGRASGFIKEMRVARMLVERGAGVTPTAVA
DVNVKLKSLIGEAGGR
>Mature_415_residues
TNDRVMAIVGAGHAGGRAAQVLRESGWGGRIVLIGAEAHLPYERPPLSKGVLTGERSAARCRLREPEAWVADRIEPVVAT
VERIDPCAREVHVSGARVFGYDALLLATGGRARRLAIPGATLDGVFALRTLDDAAALGSRLVPDARVVLIGGGFIGLEVA
ASARRRGCRVTVLDAAPRLLGRAVPETIAARVHALHEQQGVAIRVSRKPVAIERTASGALAVVLDDGDTLIADTVVAGIG
IEPADELARDAGLAVERGIVVNERLETSAHGIYAAGDVAVFPSALSGRPVRQETWHGAETQARVAARNMLGADEPYRDTP
WFWSDQYDAQLHVAGEPALGERSVARVLGDDAEIHFHFDAGARLVAASGFGRASGFIKEMRVARMLVERGAGVTPTAVAD
VNVKLKSLIGEAGGR

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=333, Percent_Identity=34.5345345345345, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI65787454, Length=333, Percent_Identity=34.5345345345345, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI226437568, Length=333, Percent_Identity=34.5345345345345, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI4757732, Length=336, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI22202629, Length=336, Percent_Identity=29.1666666666667, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI22202631, Length=198, Percent_Identity=32.8282828282828, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI195927004, Length=113, Percent_Identity=38.9380530973451, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1788892, Length=409, Percent_Identity=35.9413202933985, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789065, Length=346, Percent_Identity=29.7687861271676, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1789765, Length=291, Percent_Identity=27.8350515463918, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1789915, Length=201, Percent_Identity=29.3532338308458, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=330, Percent_Identity=30.3030303030303, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI24639257, Length=376, Percent_Identity=28.4574468085106, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281359715, Length=376, Percent_Identity=28.4574468085106, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI281359713, Length=376, Percent_Identity=28.4574468085106, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24639250, Length=376, Percent_Identity=28.4574468085106, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI18543267, Length=376, Percent_Identity=28.4574468085106, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24639252, Length=376, Percent_Identity=28.4574468085106, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24585130, Length=377, Percent_Identity=26.525198938992, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI28573993, Length=359, Percent_Identity=28.6908077994429, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24581020, Length=392, Percent_Identity=28.3163265306122, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 43828; Mature: 43697

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDRVMAIVGAGHAGGRAAQVLRESGWGGRIVLIGAEAHLPYERPPLSKGVLTGERSAA
CCCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHH
RCRLREPEAWVADRIEPVVATVERIDPCAREVHVSGARVFGYDALLLATGGRARRLAIPG
HEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEECCEEEEEECCCCEEEEECCC
ATLDGVFALRTLDDAAALGSRLVPDARVVLIGGGFIGLEVAASARRRGCRVTVLDAAPRL
CCCCCEEEEEEHHHHHHHHCCCCCCCEEEEEECCEEEHHHHHHHHHCCCEEEEECCCHHH
LGRAVPETIAARVHALHEQQGVAIRVSRKPVAIERTASGALAVVLDDGDTLIADTVVAGI
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCEEEEEECCCCEEEEHHHHHCC
GIEPADELARDAGLAVERGIVVNERLETSAHGIYAAGDVAVFPSALSGRPVRQETWHGAE
CCCCHHHHHHHCCCEEECCEEEECCCCCCCCCEEEECCCEECCCCCCCCCCCHHHCCCCC
TQARVAARNMLGADEPYRDTPWFWSDQYDAQLHVAGEPALGERSVARVLGDDAEIHFHFD
HHHHHHHHHHCCCCCCCCCCCCEECCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEC
AGARLVAASGFGRASGFIKEMRVARMLVERGAGVTPTAVADVNVKLKSLIGEAGGR
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCC
>Mature Secondary Structure 
TNDRVMAIVGAGHAGGRAAQVLRESGWGGRIVLIGAEAHLPYERPPLSKGVLTGERSAA
CCCEEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCHH
RCRLREPEAWVADRIEPVVATVERIDPCAREVHVSGARVFGYDALLLATGGRARRLAIPG
HEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEECCEEEEEECCCCEEEEECCC
ATLDGVFALRTLDDAAALGSRLVPDARVVLIGGGFIGLEVAASARRRGCRVTVLDAAPRL
CCCCCEEEEEEHHHHHHHHCCCCCCCEEEEEECCEEEHHHHHHHHHCCCEEEEECCCHHH
LGRAVPETIAARVHALHEQQGVAIRVSRKPVAIERTASGALAVVLDDGDTLIADTVVAGI
HHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCCEEEEEECCCCEEEEHHHHHCC
GIEPADELARDAGLAVERGIVVNERLETSAHGIYAAGDVAVFPSALSGRPVRQETWHGAE
CCCCHHHHHHHCCCEEECCEEEECCCCCCCCCEEEECCCEECCCCCCCCCCCHHHCCCCC
TQARVAARNMLGADEPYRDTPWFWSDQYDAQLHVAGEPALGERSVARVLGDDAEIHFHFD
HHHHHHHHHHCCCCCCCCCCCCEECCCCCCEEEECCCCCCCHHHHHHHHCCCCEEEEEEC
AGARLVAASGFGRASGFIKEMRVARMLVERGAGVTPTAVADVNVKLKSLIGEAGGR
CCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]