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The map label for this gene is paaF [H]

Identifier: 78067024

GI number: 78067024

Start: 2653215

End: 2654006

Strand: Direct

Name: paaF [H]

Synonym: Bcep18194_A5555

Alternate gene names: 78067024

Gene position: 2653215-2654006 (Clockwise)

Preceding gene: 78067022

Following gene: 78067025

Centisome position: 71.82

GC content: 67.68

Gene sequence:

>792_bases
ATGAGTGCTGAACTGCTGGCCTCGCGTCCGCCCGAGAGCGAATCGACGCTCGTCCTCACGCTATCCAATCCCGGCGCACG
CAATGCGCTGCATCCCGACATGTACGCGGCCGGTATCGAAGCGCTCGCCACCGCCGAACGCGACCCCGGGATCCGCGCGG
TCGTGCTCACCGGCGCCGATCGCTTCTTCTGCGCGGGCGGCAACCTGAACCGGCTGCTCGACAACCGCTCGAAGGATCCG
TCCTACCAGGCCGACAGCATCGATCAGCTCGCTGCATGGGTAGCGGCGATCCACGCTTCGACGAAGCCGGTGATCGCTGC
GGTAGAAGGCGCAGCCGCCGGCGCGGGCTTCTCGCTCGCGCTCGCGTGCGACCTGATCGTCGCTGCGCACGACGCGAAGT
TCGTGATGTCGTATGCACGCGTCGGCCTGACGCCCGATGGAGGCGGTTCGTGGTTCATCGCGCGCGCGCTGCCGCGTGCG
CTCGCGGCCGAGATCCTGTTCGAAGGCAAGCCTGTCGCAGCCGAACGCCTGCACACGCTCGGCGTCGTCAACCGGCTTGC
CGTGCCCGGCGCGGCACTGACCGATGCGCTGGCATGGGCCGATGCGCTCGCCGGCATCTCGCCGAACGCACTCACGCGCA
TCAAGTCGCTGCTCGACGACGCGACGATGCAACCGCTCGATGCGCATCTCGCCACCGAGCGCGATCATTTCGTCGCATCG
CTGCATCATGCGGACGGGCTCGAAGGCATCACCGCATTTCTCGAGAAACGCCCGCCCCGCTACAAGCGCTGA

Upstream 100 bases:

>100_bases
GTTCGTCACGACAACAATACATCTGCTACGGCATCGGCCGCGTCAGCACGCACGATGCGCCCGCCGCCGCAGCCCCGCCA
TCCGATGGAGACACGCTCGC

Downstream 100 bases:

>100_bases
GCCGCCGCGCCGGCTATTGAATAGACGCATCGCCGCCGCATCAACCCGATGCGGCGCGGCGCCCCCATCCAGACGACTCG
GACGACATGTGATTGCCCTC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSAELLASRPPESESTLVLTLSNPGARNALHPDMYAAGIEALATAERDPGIRAVVLTGADRFFCAGGNLNRLLDNRSKDP
SYQADSIDQLAAWVAAIHASTKPVIAAVEGAAAGAGFSLALACDLIVAAHDAKFVMSYARVGLTPDGGGSWFIARALPRA
LAAEILFEGKPVAAERLHTLGVVNRLAVPGAALTDALAWADALAGISPNALTRIKSLLDDATMQPLDAHLATERDHFVAS
LHHADGLEGITAFLEKRPPRYKR

Sequences:

>Translated_263_residues
MSAELLASRPPESESTLVLTLSNPGARNALHPDMYAAGIEALATAERDPGIRAVVLTGADRFFCAGGNLNRLLDNRSKDP
SYQADSIDQLAAWVAAIHASTKPVIAAVEGAAAGAGFSLALACDLIVAAHDAKFVMSYARVGLTPDGGGSWFIARALPRA
LAAEILFEGKPVAAERLHTLGVVNRLAVPGAALTDALAWADALAGISPNALTRIKSLLDDATMQPLDAHLATERDHFVAS
LHHADGLEGITAFLEKRPPRYKR
>Mature_262_residues
SAELLASRPPESESTLVLTLSNPGARNALHPDMYAAGIEALATAERDPGIRAVVLTGADRFFCAGGNLNRLLDNRSKDPS
YQADSIDQLAAWVAAIHASTKPVIAAVEGAAAGAGFSLALACDLIVAAHDAKFVMSYARVGLTPDGGGSWFIARALPRAL
AAEILFEGKPVAAERLHTLGVVNRLAVPGAALTDALAWADALAGISPNALTRIKSLLDDATMQPLDAHLATERDHFVASL
HHADGLEGITAFLEKRPPRYKR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=258, Percent_Identity=30.6201550387597, Blast_Score=117, Evalue=8e-27,
Organism=Homo sapiens, GI31542718, Length=242, Percent_Identity=29.7520661157025, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI221136756, Length=246, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI221136753, Length=246, Percent_Identity=25.609756097561, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI221307494, Length=248, Percent_Identity=26.6129032258064, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=28.7937743190661, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI116235487, Length=204, Percent_Identity=28.4313725490196, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI25453481, Length=245, Percent_Identity=24.4897959183673, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI51538573, Length=245, Percent_Identity=24.4897959183673, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI70995211, Length=239, Percent_Identity=28.8702928870293, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4757966, Length=204, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI51538581, Length=204, Percent_Identity=25, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI4757968, Length=251, Percent_Identity=25.0996015936255, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI49355787, Length=251, Percent_Identity=25.0996015936255, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI260274832, Length=240, Percent_Identity=24.5833333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI45643119, Length=240, Percent_Identity=24.5833333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI260275230, Length=240, Percent_Identity=24.5833333333333, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=25.5681818181818, Blast_Score=65, Evalue=9e-11,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=36.7816091954023, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1787660, Length=254, Percent_Identity=37.007874015748, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI221142681, Length=253, Percent_Identity=32.8063241106719, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788597, Length=251, Percent_Identity=32.2709163346614, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87082183, Length=244, Percent_Identity=27.4590163934426, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788682, Length=184, Percent_Identity=30.9782608695652, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=254, Percent_Identity=31.1023622047244, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=31.0756972111554, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17540306, Length=262, Percent_Identity=26.3358778625954, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17540714, Length=227, Percent_Identity=25.9911894273128, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=225, Percent_Identity=27.5555555555556, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17535521, Length=245, Percent_Identity=25.3061224489796, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=257, Percent_Identity=23.3463035019455, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=250, Percent_Identity=26.4, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17558304, Length=165, Percent_Identity=25.4545454545455, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI20129971, Length=251, Percent_Identity=30.6772908366534, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24653477, Length=251, Percent_Identity=30.6772908366534, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=31.6, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=29.7959183673469, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24654903, Length=243, Percent_Identity=24.2798353909465, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19920382, Length=212, Percent_Identity=29.2452830188679, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=253, Percent_Identity=23.7154150197628, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 27600; Mature: 27468

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAELLASRPPESESTLVLTLSNPGARNALHPDMYAAGIEALATAERDPGIRAVVLTGAD
CCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCC
RFFCAGGNLNRLLDNRSKDPSYQADSIDQLAAWVAAIHASTKPVIAAVEGAAAGAGFSLA
EEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
LACDLIVAAHDAKFVMSYARVGLTPDGGGSWFIARALPRALAAEILFEGKPVAAERLHTL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GVVNRLAVPGAALTDALAWADALAGISPNALTRIKSLLDDATMQPLDAHLATERDHFVAS
HHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHH
LHHADGLEGITAFLEKRPPRYKR
HHHCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SAELLASRPPESESTLVLTLSNPGARNALHPDMYAAGIEALATAERDPGIRAVVLTGAD
CCCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCC
RFFCAGGNLNRLLDNRSKDPSYQADSIDQLAAWVAAIHASTKPVIAAVEGAAAGAGFSLA
EEEEECCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHH
LACDLIVAAHDAKFVMSYARVGLTPDGGGSWFIARALPRALAAEILFEGKPVAAERLHTL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
GVVNRLAVPGAALTDALAWADALAGISPNALTRIKSLLDDATMQPLDAHLATERDHFVAS
HHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHH
LHHADGLEGITAFLEKRPPRYKR
HHHCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]