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The map label for this gene is gcvA [H]

Identifier: 78066955

GI number: 78066955

Start: 2572619

End: 2573560

Strand: Direct

Name: gcvA [H]

Synonym: Bcep18194_A5486

Alternate gene names: 78066955

Gene position: 2572619-2573560 (Clockwise)

Preceding gene: 78066952

Following gene: 78066956

Centisome position: 69.64

GC content: 72.19

Gene sequence:

>942_bases
ATGTCAACGCCGCTCGTCCGTCTCCCGTCGCTCGACCTCGTCCGCGGTTTCGTCGCCGTCGGCCGCCGCATGAGCATCAC
GCTCGCCGCGCAGGACCTGTGCGTCACGCAATCGGCGGTCAGCCGCCAGGTGCACGCACTCGAAGCGCATCTCGGTGTCG
CGCTGCTGCAGCGCGGCTACCGGAAGATCGCGTTCACACCCGAAGGCGAGCGGCTGTTTCGTGTCGCGGATGCGGCACTG
CACGGGCTGCAGGACACCGTCGCGTCGCTCGCCGCCGCGCGCGAGCGCCAGCCCGTCACGATCACCGCGAGCATCGGCGT
CACCGCGCTGTGGCTGCTGCCGCGGCTCGGGCGGCTGCAGGCGCGCCTGCCGGAGATCGACTTACGCGTCGCCGCGAACG
ACAAGGTACTCGATTTGCGCGCCGAAGGCATCGACCTCGGGATCCGCTATTGTCCGCGCGACCGCGCGCCGGCCGGCGCG
CTGCGGCTGTTCGACGAAATCGTCGTGCCGGTCGCGCATCCGGCGCTCGCCGCGCGGCCCGTCACGAACGCGGCCGCGAT
CGCCGACCACGTGCTGCTCGAATTCGACGGGCCGCCGCAGCCGCAGTTGCAGTGGCGCGCACACCTGCACGCGGCCGGGC
TCGGCGACGCGCGCCCGAAAGGCGTGCTGCGTTTCAACCAGTACGACCAGGTGATCCAGGCCGCGATCGCGGGCCAGGGC
GTCGCGCTCGGCCGCCTCGCGCTCGTCGCGCCGATGCTCGCGGACGGGCGGCTCGCGGTGCTCGGCCCACACACGCAGGC
GCTGTCGGATGCGTACGGCTACTGGCTGTTCCAGCACGATCCGGCCCCGCGCCGCGAAGTTGCCGACGTCCGCGACTGGA
TGCTCGCCGAAGCGGCCGAATGCGATGCCGCGATGCGTGCGCACGGCCCGACTTCGGCGTAA

Upstream 100 bases:

>100_bases
CTCCCGCCCCATCCTTCGCCTTCCACACCAGCCTCCATTGAACTTGACGCGCCCGGCGTTCTCAACCAATCTTGCTGCTC
CGTTCGCATGAGTCACGCGC

Downstream 100 bases:

>100_bases
GCCGGCGCAACGGTGACAAACGAAGCTGACCGTTGCCGGGCGCTCCCGCCCCACTCCGCACGCCCCGCGTCGCATTCCCT
GCACGCATGCCAACCTGACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGYRKIAFTPEGERLFRVADAAL
HGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPEIDLRVAANDKVLDLRAEGIDLGIRYCPRDRAPAGA
LRLFDEIVVPVAHPALAARPVTNAAAIADHVLLEFDGPPQPQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
VALGRLALVAPMLADGRLAVLGPHTQALSDAYGYWLFQHDPAPRREVADVRDWMLAEAAECDAAMRAHGPTSA

Sequences:

>Translated_313_residues
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGYRKIAFTPEGERLFRVADAAL
HGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPEIDLRVAANDKVLDLRAEGIDLGIRYCPRDRAPAGA
LRLFDEIVVPVAHPALAARPVTNAAAIADHVLLEFDGPPQPQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
VALGRLALVAPMLADGRLAVLGPHTQALSDAYGYWLFQHDPAPRREVADVRDWMLAEAAECDAAMRAHGPTSA
>Mature_312_residues
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGYRKIAFTPEGERLFRVADAALH
GLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQARLPEIDLRVAANDKVLDLRAEGIDLGIRYCPRDRAPAGAL
RLFDEIVVPVAHPALAARPVTNAAAIADHVLLEFDGPPQPQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQGV
ALGRLALVAPMLADGRLAVLGPHTQALSDAYGYWLFQHDPAPRREVADVRDWMLAEAAECDAAMRAHGPTSA

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=31.023102310231, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1788706, Length=259, Percent_Identity=30.8880308880309, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786448, Length=291, Percent_Identity=31.2714776632302, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786401, Length=254, Percent_Identity=27.9527559055118, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081978, Length=266, Percent_Identity=30.4511278195489, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787589, Length=232, Percent_Identity=30.1724137931034, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693193, Length=247, Percent_Identity=27.1255060728745, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=272, Percent_Identity=29.7794117647059, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33708; Mature: 33577

Theoretical pI: Translated: 8.51; Mature: 8.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGY
CCCCCEECCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKIAFTPEGERLFRVADAALHGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
ARLPEIDLRVAANDKVLDLRAEGIDLGIRYCPRDRAPAGALRLFDEIVVPVAHPALAARP
HCCCCEEEEEECCCCEEEEEECCEEECEEECCCCCCCHHHHHHHHHHHHHHCCCHHHCCC
VTNAAAIADHVLLEFDGPPQPQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
CCHHHHHHHEEEEEECCCCCCCHHHHHHEEECCCCCCCCCCEEEECHHHHHHHHHHHCCC
VALGRLALVAPMLADGRLAVLGPHTQALSDAYGYWLFQHDPAPRREVADVRDWMLAEAAE
HHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHH
CDAAMRAHGPTSA
HHHHHHCCCCCCC
>Mature Secondary Structure 
STPLVRLPSLDLVRGFVAVGRRMSITLAAQDLCVTQSAVSRQVHALEAHLGVALLQRGY
CCCCEECCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RKIAFTPEGERLFRVADAALHGLQDTVASLAAARERQPVTITASIGVTALWLLPRLGRLQ
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHH
ARLPEIDLRVAANDKVLDLRAEGIDLGIRYCPRDRAPAGALRLFDEIVVPVAHPALAARP
HCCCCEEEEEECCCCEEEEEECCEEECEEECCCCCCCHHHHHHHHHHHHHHCCCHHHCCC
VTNAAAIADHVLLEFDGPPQPQLQWRAHLHAAGLGDARPKGVLRFNQYDQVIQAAIAGQG
CCHHHHHHHEEEEEECCCCCCCHHHHHHEEECCCCCCCCCCEEEECHHHHHHHHHHHCCC
VALGRLALVAPMLADGRLAVLGPHTQALSDAYGYWLFQHDPAPRREVADVRDWMLAEAAE
HHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHH
CDAAMRAHGPTSA
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]