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The map label for this gene is mls [H]

Identifier: 78066830

GI number: 78066830

Start: 2421046

End: 2422638

Strand: Direct

Name: mls [H]

Synonym: Bcep18194_A5361

Alternate gene names: 78066830

Gene position: 2421046-2422638 (Clockwise)

Preceding gene: 78066829

Following gene: 78066832

Centisome position: 65.54

GC content: 65.29

Gene sequence:

>1593_bases
ATGAGCACCCCGATCACGCTGCCGCAAGGCATGGCGATCACCGGCGAAATCAAGCCGGGTTACGAAGCAATCCTGACGCC
TGAAGCGCTCGAACTCGTCGCAGCGCTGCACCGCACGTTCGAGCCGCGCCGCCAGGCGCTGCTGCAGGCGCGCGTGGAGC
GCACGAAACGCCTCGACGCGGGCGAACGCCCCGACTTCCTGGCCGAGACGAAGGCGATCCGCGAAGGCGACTGGAAGGTC
GCGCCGCTGCCGGCCGACCTGCAATGCCGTCGTGTCGAGATCACGGGCCCCGTCGAGCGCAAGATGATCATCAACGCGCT
GAACTCGGGCGCGGATTCGTACATGACGGACTTCGAGGATTCGAACGCGCCGAGCTGGACGAACCAGATCGACGGCCAGA
TCAACCTGAAGGACGCGGTGCGCCGCACGATCTCGCTCGAGCAGAACGGCAAGTCGTACCAGCTGAACGACAAGGTCGCG
ACGCTGATCGTGCGTCCGCGCGGCTGGCACCTCGACGAGAAGCACGTGACGGTCGACGGCCAGCGCGTCTCCGGCGGCAT
TTTCGATTTCGCGCTGTTCCTGTTCCACAACGCGAAGGAACTGCTCGCGCGCGGCTCGGGCCCGTACTTCTACCTGCCGA
AGATGGAGAGCCATCTCGAGGCACGCCTGTGGAACGACATCTTCGTCGCCGCGCAGGAAGGCGTCGGCGTGCCGCGCGGC
ACGATCCGCGCGACGGTGCTGATCGAGACGATCCTCGCCGCGTTCGAGATGGACGAGATCCTGTACGAACTGCGCGAACA
CAGCTCGGGCCTGAACGCCGGCCGCTGGGACTACATCTTCTCGGCCATCAAGAAGTTCAAGAACGACCGCGACTTCTGCC
TCGCCGAGCGTTCGAAGATCACGATGACCGTGCCGTTCATGCGCGCGTATGCGCTGCTGCTGCTGAAGACCTGCCACAAG
CGCAACGCGCCGGCGATCGGCGGGATGAGCGCGCTGATCCCGATCAAGAACGATCCGGAAGCGAACGACAAGGCGATGGG
CGGCGTGCGCTCGGACAAGCAGCGCGACGCGACCGACGGCTACGACGGCGGCTGGGTCGCGCACCCGGGCCTCGTGCCGA
TCGCGATGGAAGAGTTCGTCAAGGTGCTCGGCGACAAGCCGAACCAGATCGCGAAGCAGCGCGACGACGTGCAGGTCGAA
GGCAAGAACCTGCTCGACTTCCAGCCCGAAGCGCCGATCACCGAAGCCGGCCTGCGCAACAACATCAACGTCGGCATCCA
CTACCTCGGCGCATGGCTCGACGGCAACGGCTGCGTGCCGATCCACAACCTGATGGAAGATGCGGCCACGGCCGAAATCT
CCCGCTCGCAGGTGTGGCAATGGATCCGCTCGCCGAAGGGTGTGCTCGACGACGGCCGCAAGGTCACCGCCGAACTCGTG
CGTGAACTCTCGAAGGCCGAGCTGGACAACGTGAAGCGCTCGGTCGGCGGCAACACGCAGCCGTACGAGCGCGCCGCGGC
GATCTTCGAGCAGATGTCGACGTCGGAAGGCTTCACCGAATTCCTGACGCTGCCGCTGTACGAGGAAATCTGA

Upstream 100 bases:

>100_bases
GCAAACCGCACGCGCCCCAGCCCGGGTGCATGACGTTCGCAGCGCTGCCGCCTTCCCCCTTCACCGAAACCGCAACTGAC
CGACCAAGGAGATGAGACTC

Downstream 100 bases:

>100_bases
CGTCGCTGCCCGCTACGATCGGAGCCGGCCCTGCGTCGGCGCCGGTCGGATGCGATGACGGCATCAAAAAAAGCGCCCCG
AGGGGCGCTTTTTCATTTGC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSTPITLPQGMAITGEIKPGYEAILTPEALELVAALHRTFEPRRQALLQARVERTKRLDAGERPDFLAETKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYQLNDKVA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEGVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLAERSKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQVE
GKNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
RELSKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Sequences:

>Translated_530_residues
MSTPITLPQGMAITGEIKPGYEAILTPEALELVAALHRTFEPRRQALLQARVERTKRLDAGERPDFLAETKAIREGDWKV
APLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYQLNDKVA
TLIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEGVGVPRG
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLAERSKITMTVPFMRAYALLLLKTCHK
RNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQVE
GKNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
RELSKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
>Mature_529_residues
STPITLPQGMAITGEIKPGYEAILTPEALELVAALHRTFEPRRQALLQARVERTKRLDAGERPDFLAETKAIREGDWKVA
PLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFEDSNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYQLNDKVAT
LIVRPRGWHLDEKHVTVDGQRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEGVGVPRGT
IRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLAERSKITMTVPFMRAYALLLLKTCHKR
NAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDGYDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQVEG
KNLLDFQPEAPITEAGLRNNINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELVR
ELSKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=536, Percent_Identity=49.6268656716418, Blast_Score=514, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI17561814, Length=524, Percent_Identity=51.1450381679389, Blast_Score=526, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71982926, Length=420, Percent_Identity=53.0952380952381, Blast_Score=442, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6324212, Length=514, Percent_Identity=52.7237354085603, Blast_Score=539, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6322222, Length=514, Percent_Identity=50.9727626459144, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 59141; Mature: 59010

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPITLPQGMAITGEIKPGYEAILTPEALELVAALHRTFEPRRQALLQARVERTKRLDA
CCCCCCCCCCCEEEECCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GERPDFLAETKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYQLNDKVATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEGVGVPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHEEECCCCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLAERSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDG
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQVEGKNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RELSKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC
>Mature Secondary Structure 
STPITLPQGMAITGEIKPGYEAILTPEALELVAALHRTFEPRRQALLQARVERTKRLDA
CCCCCCCCCCEEEECCCCCCCEEECHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
GERPDFLAETKAIREGDWKVAPLPADLQCRRVEITGPVERKMIINALNSGADSYMTDFED
CCCCCHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCHHHHHHHHHHHCCHHHHHHCCCC
SNAPSWTNQIDGQINLKDAVRRTISLEQNGKSYQLNDKVATLIVRPRGWHLDEKHVTVDG
CCCCCCHHCCCCEEEHHHHHHHHHHHCCCCCCEEECCCEEEEEECCCCCCCCCCEEEECC
QRVSGGIFDFALFLFHNAKELLARGSGPYFYLPKMESHLEARLWNDIFVAAQEGVGVPRG
CCCCCHHHHHHHHHHHCHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHEEECCCCCCCCC
TIRATVLIETILAAFEMDEILYELREHSSGLNAGRWDYIFSAIKKFKNDRDFCLAERSKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCEE
TMTVPFMRAYALLLLKTCHKRNAPAIGGMSALIPIKNDPEANDKAMGGVRSDKQRDATDG
EEEEHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHHCCCCCCCCCCCCCC
YDGGWVAHPGLVPIAMEEFVKVLGDKPNQIAKQRDDVQVEGKNLLDFQPEAPITEAGLRN
CCCCEEECCCCCHHHHHHHHHHHCCCCHHHHHCCCCEEECCCCCCCCCCCCCCHHHCCCC
NINVGIHYLGAWLDGNGCVPIHNLMEDAATAEISRSQVWQWIRSPKGVLDDGRKVTAELV
CCCEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
RELSKAELDNVKRSVGGNTQPYERAAAIFEQMSTSEGFTEFLTLPLYEEI
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA