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The map label for this gene is pncA [H]

Identifier: 78066675

GI number: 78066675

Start: 2252995

End: 2253627

Strand: Reverse

Name: pncA [H]

Synonym: Bcep18194_A5206

Alternate gene names: 78066675

Gene position: 2253627-2252995 (Counterclockwise)

Preceding gene: 78066676

Following gene: 78066674

Centisome position: 61.01

GC content: 70.93

Gene sequence:

>633_bases
ATGAAACGCACCGACGACGTACTGCTCGTGATCGACGTGCAATACGACTTCATGCCGGGCGGCGCGCTCGCGGTGCCCGA
CGGCGACGCGGTCGTGCCGGTGATCAACGCGCTGGCGCAACGCTTCGACCAGGTCGTGCTGACGCAGGACTGGCACCCGC
GCGAGCACGTGTCGTTCGCGGCGAACCACCCGGGCCGCGAACCGTTCTCCACGCTCGCGCTGCCGTACGGCGAGCAGGTG
CTGTGGCCCGTGCACTGCGTGCAGGACACCGACGGCGCGGCGTTGCATCGCGACCTCGACATCCCGCACGCGCGGCTCGT
GATCCGCAAGGGCGGCGACGCGCAGGTCGACAGCTATTCCGCGTTCGTCGAAGCCGACCGCACGACGCGCACCGGGCTCG
CGGGCTATCTGCGCGAGCTGGGCGCGAAGCGCGTGTGGTGCTGCGGGCTCGCGACCGACTACTGCGTCGCGTGGTCGGCG
CTCGATGCGCGCGCGGCCGGTTTCGAAGCCGCAGTGATCAACGATGCGTGCCGCGCGATCGACCTGAACGGGTCGCTCGC
CCACGCATGGCAACAGATGCAGGCAGCGGGCGTCGCGCATGTGACGTCCGCGGGCGCGCGCCCGCAGGCGTAG

Upstream 100 bases:

>100_bases
TGGCAGCTCGCGCCGTCGATCGCGGAGCTCGCGAAGGCGGGCCGCGGGTTCAACGGCTGACGCGCCGGATCGCGGCGCGA
CGCACACCCGGAGAAATGCA

Downstream 100 bases:

>100_bases
CGCATTCCCCATCCGATTCAGGACATGATGCAGGACCGAAGGAGACTCGCATGACCGAAGCCGTAATCGTATCGACCGCC
CGCACGCCGCTGGCGAAATC

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQRFDQVVLTQDWHPREHVSFAANHPGREPFSTLALPYGEQV
LWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYSAFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVINDACRAIDLNGSLAHAWQQMQAAGVAHVTSAGARPQA

Sequences:

>Translated_210_residues
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQRFDQVVLTQDWHPREHVSFAANHPGREPFSTLALPYGEQV
LWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYSAFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVINDACRAIDLNGSLAHAWQQMQAAGVAHVTSAGARPQA
>Mature_210_residues
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQRFDQVVLTQDWHPREHVSFAANHPGREPFSTLALPYGEQV
LWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYSAFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSA
LDARAAGFEAAVINDACRAIDLNGSLAHAWQQMQAAGVAHVTSAGARPQA

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=202, Percent_Identity=43.5643564356436, Blast_Score=152, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI193206735, Length=224, Percent_Identity=33.4821428571429, Blast_Score=103, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI17543130, Length=224, Percent_Identity=33.9285714285714, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI71999339, Length=224, Percent_Identity=32.5892857142857, Blast_Score=97, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6321401, Length=189, Percent_Identity=34.9206349206349, Blast_Score=114, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24648247, Length=217, Percent_Identity=34.5622119815668, Blast_Score=124, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22671; Mature: 22671

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQRFDQVVLTQDWHPREHVSFA
CCCCCCEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHCEEE
ANHPGREPFSTLALPYGEQVLWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYS
CCCCCCCCHHHEECCCCCCEEEEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCHH
AFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSALDARAAGFEAAVINDACRAI
HEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHEECCCHHEE
DLNGSLAHAWQQMQAAGVAHVTSAGARPQA
ECCCHHHHHHHHHHHCCHHHHHCCCCCCCC
>Mature Secondary Structure
MKRTDDVLLVIDVQYDFMPGGALAVPDGDAVVPVINALAQRFDQVVLTQDWHPREHVSFA
CCCCCCEEEEEEEEEEECCCCEEECCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHCEEE
ANHPGREPFSTLALPYGEQVLWPVHCVQDTDGAALHRDLDIPHARLVIRKGGDAQVDSYS
CCCCCCCCHHHEECCCCCCEEEEEEEEECCCCCEEEECCCCCCEEEEEECCCCCCCCCHH
AFVEADRTTRTGLAGYLRELGAKRVWCCGLATDYCVAWSALDARAAGFEAAVINDACRAI
HEEECCCCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHCCCHHHEECCCHHEE
DLNGSLAHAWQQMQAAGVAHVTSAGARPQA
ECCCHHHHHHHHHHHCCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]