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The map label for this gene is acoA [H]

Identifier: 78066609

GI number: 78066609

Start: 2185396

End: 2186379

Strand: Reverse

Name: acoA [H]

Synonym: Bcep18194_A5140

Alternate gene names: 78066609

Gene position: 2186379-2185396 (Counterclockwise)

Preceding gene: 78066610

Following gene: 78066608

Centisome position: 59.19

GC content: 66.26

Gene sequence:

>984_bases
ATGACCGCTTCGACACAGCTCAGCAGGGACAAGCTGCTGGACGCCTACCGGCTGATGCGCACGATCCGCGAATTCGAGGA
ACGCCTGCACGTCGAATTCGCGACCGGCGAGATCCCCGGCTTCGTTCACCTGTATGCGGGCGAGGAGGCGTCCGCGGTCG
GCACGATACTGCACCTCGGCCTCGACGACTACGTTGCGACCACGCACCGCGGCCACGGCCATTGCATCGCGAAGGGCGTC
GACGTGCACGGGATGATGGCCGAGATCTACGGCAAGAAGACGGGCGTCTGCCACGGCAAGGGCGGCTCGATGCACATCGC
CGACCTGGCGATGGGGATGCTCGGCGCGAACGGGATCGTCGGTGCGGGCGGCCCGCTCGTCTGCGGTGCGGCGCTCGCGG
CGAAGCACAAGAAGACCGGCGGCGTCGGCGTGTGCTTCTTCGGCGACGGCGCATCGAACCAGGGCGTGATCTTCGAATCG
ATGAACCTTGCGTCGGTGTGGCGGCTGCCCGCGATCTTCGTGGCCGAAAACAACGGCTATGCGGAAGCGACGTCGTCGAG
CTGGTCGGTCGCCACCGACAACATCGCCGATCGTGCGAACGGGTTCGGGATGCCGGGCGTGATCGTCGACGGCTTCGACT
TCTTCGCGGTGCACGAAGCGCTTGGCGAAGCCGTCGAGCGTGCGCGCAACGGTGGCGGCCCGACGCTCGTCGAGGTGAAG
TTCACGCGCTATTTCGGTCACTTCGAAGGTGATGCGCAAACCTATCGCGCACCGGGTGAAGTGCAGAAGCTGCGCGACGA
GAAGGACTGCCTGAAGCATTTCGAAACGCGCGTCGTGCGGGCCGAAGCATTGACGACCGCCGACCTGCGCGCGGTCGATG
CCCAGGTGAAGGCGCTGATCGACGATGCGGTCGCGCAGGCCAAGGCCGCGCCGGTACCCGATGCCGCCGACCTGCTGACC
GACGTCTACGTGTCGTACCCGTGA

Upstream 100 bases:

>100_bases
ACGGCGGCTTCACGTTCCACTGACGGCCGCAGGCCGTGAAATCCGTCGCCACGGCCCGGCCGCGGCGGCATTCGCAGTCA
GACCTACCCAGGAGACACAC

Downstream 100 bases:

>100_bases
GCGCGGCCTGACACTGCGGCCGACACGGCGAACCGAACATTCCAGGAGACAGACATGGCAAGGAAGATCACTTATTCGCA
GGCGATCAACGAAGCGCTCG

Product: pyruvate dehydrogenase (lipoamide)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTASTQLSRDKLLDAYRLMRTIREFEERLHVEFATGEIPGFVHLYAGEEASAVGTILHLGLDDYVATTHRGHGHCIAKGV
DVHGMMAEIYGKKTGVCHGKGGSMHIADLAMGMLGANGIVGAGGPLVCGAALAAKHKKTGGVGVCFFGDGASNQGVIFES
MNLASVWRLPAIFVAENNGYAEATSSSWSVATDNIADRANGFGMPGVIVDGFDFFAVHEALGEAVERARNGGGPTLVEVK
FTRYFGHFEGDAQTYRAPGEVQKLRDEKDCLKHFETRVVRAEALTTADLRAVDAQVKALIDDAVAQAKAAPVPDAADLLT
DVYVSYP

Sequences:

>Translated_327_residues
MTASTQLSRDKLLDAYRLMRTIREFEERLHVEFATGEIPGFVHLYAGEEASAVGTILHLGLDDYVATTHRGHGHCIAKGV
DVHGMMAEIYGKKTGVCHGKGGSMHIADLAMGMLGANGIVGAGGPLVCGAALAAKHKKTGGVGVCFFGDGASNQGVIFES
MNLASVWRLPAIFVAENNGYAEATSSSWSVATDNIADRANGFGMPGVIVDGFDFFAVHEALGEAVERARNGGGPTLVEVK
FTRYFGHFEGDAQTYRAPGEVQKLRDEKDCLKHFETRVVRAEALTTADLRAVDAQVKALIDDAVAQAKAAPVPDAADLLT
DVYVSYP
>Mature_326_residues
TASTQLSRDKLLDAYRLMRTIREFEERLHVEFATGEIPGFVHLYAGEEASAVGTILHLGLDDYVATTHRGHGHCIAKGVD
VHGMMAEIYGKKTGVCHGKGGSMHIADLAMGMLGANGIVGAGGPLVCGAALAAKHKKTGGVGVCFFGDGASNQGVIFESM
NLASVWRLPAIFVAENNGYAEATSSSWSVATDNIADRANGFGMPGVIVDGFDFFAVHEALGEAVERARNGGGPTLVEVKF
TRYFGHFEGDAQTYRAPGEVQKLRDEKDCLKHFETRVVRAEALTTADLRAVDAQVKALIDDAVAQAKAAPVPDAADLLTD
VYVSYP

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=323, Percent_Identity=38.0804953560372, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI291084742, Length=325, Percent_Identity=38.1538461538462, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI4505685, Length=325, Percent_Identity=38.1538461538462, Blast_Score=200, Evalue=1e-51,
Organism=Homo sapiens, GI291084744, Length=332, Percent_Identity=37.3493975903614, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI291084757, Length=325, Percent_Identity=34.1538461538462, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI11386135, Length=323, Percent_Identity=28.4829721362229, Blast_Score=117, Evalue=1e-26,
Organism=Homo sapiens, GI258645172, Length=323, Percent_Identity=28.4829721362229, Blast_Score=114, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17536047, Length=325, Percent_Identity=38.7692307692308, Blast_Score=219, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI32564172, Length=325, Percent_Identity=38.7692307692308, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI86563355, Length=317, Percent_Identity=26.813880126183, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI86563357, Length=317, Percent_Identity=26.813880126183, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6321026, Length=324, Percent_Identity=37.962962962963, Blast_Score=208, Evalue=9e-55,
Organism=Drosophila melanogaster, GI24639740, Length=319, Percent_Identity=38.871473354232, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24639744, Length=319, Percent_Identity=38.871473354232, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI28571106, Length=319, Percent_Identity=38.871473354232, Blast_Score=211, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24639746, Length=306, Percent_Identity=39.2156862745098, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24639748, Length=326, Percent_Identity=38.9570552147239, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI21355903, Length=331, Percent_Identity=26.2839879154079, Blast_Score=101, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 34731; Mature: 34599

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASTQLSRDKLLDAYRLMRTIREFEERLHVEFATGEIPGFVHLYAGEEASAVGTILHLG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCHHHHHHHEEC
LDDYVATTHRGHGHCIAKGVDVHGMMAEIYGKKTGVCHGKGGSMHIADLAMGMLGANGIV
CHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHCCCCCC
GAGGPLVCGAALAAKHKKTGGVGVCFFGDGASNQGVIFESMNLASVWRLPAIFVAENNGY
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCHHHHHCCCEEEEECCCCC
AEATSSSWSVATDNIADRANGFGMPGVIVDGFDFFAVHEALGEAVERARNGGGPTLVEVK
EEECCCCCCEECCCHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
FTRYFGHFEGDAQTYRAPGEVQKLRDEKDCLKHFETRVVRAEALTTADLRAVDAQVKALI
EEEEECCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDAVAQAKAAPVPDAADLLTDVYVSYP
HHHHHHHCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TASTQLSRDKLLDAYRLMRTIREFEERLHVEFATGEIPGFVHLYAGEEASAVGTILHLG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCEEEEEECCCCHHHHHHHEEC
LDDYVATTHRGHGHCIAKGVDVHGMMAEIYGKKTGVCHGKGGSMHIADLAMGMLGANGIV
CHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCCEEECCCCCEEHHHHHHHHHCCCCCC
GAGGPLVCGAALAAKHKKTGGVGVCFFGDGASNQGVIFESMNLASVWRLPAIFVAENNGY
CCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCCHHHHHCCCEEEEECCCCC
AEATSSSWSVATDNIADRANGFGMPGVIVDGFDFFAVHEALGEAVERARNGGGPTLVEVK
EEECCCCCCEECCCHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
FTRYFGHFEGDAQTYRAPGEVQKLRDEKDCLKHFETRVVRAEALTTADLRAVDAQVKALI
EEEEECCCCCCCHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DDAVAQAKAAPVPDAADLLTDVYVSYP
HHHHHHHCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]