Click here to switch to the map view.

The map label for this gene is rpoS [H]

Identifier: 78066590

GI number: 78066590

Start: 2164423

End: 2165511

Strand: Reverse

Name: rpoS [H]

Synonym: Bcep18194_A5121

Alternate gene names: 78066590

Gene position: 2165511-2164423 (Counterclockwise)

Preceding gene: 78066591

Following gene: 78066589

Centisome position: 58.62

GC content: 65.84

Gene sequence:

>1089_bases
ATGCCGAAATCGAAGCGCCACGAGCCGCAAGCCGAGTCTGAGAAAATCAGTCGAGCCACGCAAGCATCGGTGGGGCGAGC
TGGTGCTTCGACGGACGACGAAGACGACGTCGCGGACAACGAGCGTGACCTCGAGGCACGCGACTCTGACTCGGAAGGCG
GCGACGACGAGCGCGAAGGCCGTGCCGAGGCGGCGCCCGACGTCGACGATTTCCGCACGATGCTGCAGGCCGAACTCACG
GCCGACACGATCCAGCATTACCTGAACCGCATCAGCGTGAAGCCGCTGCTCACCGTCGAGGAGGAGCAGCGTTATTCGCG
TCTGGCCAAGGCCGGCGAATTCGAGGCGCGGCAGGTGATGATCGAGCGCAACCTGCGCCTCGTCGTCAGCATCGCGAAGG
GGTATCTGAACCGTGGCGTGCCGCTTCTCGACCTGATCGAGGAAGGCAACCTCGGCCTGATGCATGCGATCGAGAAATTC
GACCCGACGCGCGGCTTTCGTTTCTCGACCTACGCGACGTGGTGGATCCGGCAGAGCATCGAACGGGCGATCATGAACCA
GGCCCGCACGGTGCGCCTGCCCGTGCACGTGATCCGCGAACTGAACCAGGTGCTGCGCGCGAAGCGCCATCTCGAAAAGA
ACTCGATGTCGACGGGCGAGGCGGCCGAGCGCCGCGAAGCCAGCATCGACGACATCGCCTATCTCACCGGCAAGACCGCC
GAGGAAGTCACCGACATCCTCGCGCTCAACGAGCACACGGCGTCGCTCGACGCGCCGCTGGACCTCGATCCCGCGAGCAG
CCTGCTCGACCTGCTGCCCGACGACCAGAGCCAGTCGCCTGACGCCGAGGTGCAGCACCGCGAGCTCGAGACGCTGACGC
GCGCGTGGCTGTCTCGGCTGTCCGACAAGCACCGGCACGTGATCGAGCGCCGCTTCGGCCTGAACCACATCGAACCGGCG
ACGCTCGAGGAGCTGGCCGACGAGATGGGGCTCACGCGCGAGCGCGTGCGCCAGATCCAGCAGGAAGCGCTGGTGCGCCT
CAAGCGGTTTTTCGCCTCCAACGGTGTGCGCAAGGACGCCGTTCTGTAA

Upstream 100 bases:

>100_bases
ACAGCGATTCCGACCGCGTGATGCTGCATTTCGAGGTTCGCCGGCAGGGTAAACCTGTCGATCCGCTGAAGTATTTGCCG
CCTCAATAACCGAGACGACC

Downstream 100 bases:

>100_bases
CTGATGACTCCGATTCTTGTTTTTGACATCGAGACCATTCCCGATGTCGACGGCATTCGCCGTCTGGAAGACCTGCCCGC
GACGCTCGACGATGCCGCGG

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MPKSKRHEPQAESEKISRATQASVGRAGASTDDEDDVADNERDLEARDSDSEGGDDEREGRAEAAPDVDDFRTMLQAELT
ADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKF
DPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTA
EEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEPA
TLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Sequences:

>Translated_362_residues
MPKSKRHEPQAESEKISRATQASVGRAGASTDDEDDVADNERDLEARDSDSEGGDDEREGRAEAAPDVDDFRTMLQAELT
ADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKF
DPTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTA
EEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEPA
TLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL
>Mature_361_residues
PKSKRHEPQAESEKISRATQASVGRAGASTDDEDDVADNERDLEARDSDSEGGDDEREGRAEAAPDVDDFRTMLQAELTA
DTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVMIERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFD
PTRGFRFSTYATWWIRQSIERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTAE
EVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRLSDKHRHVIERRFGLNHIEPAT
LEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDAVL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=281, Percent_Identity=50.1779359430605, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789448, Length=264, Percent_Identity=37.8787878787879, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789871, Length=276, Percent_Identity=28.6231884057971, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41076; Mature: 40945

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKSKRHEPQAESEKISRATQASVGRAGASTDDEDDVADNERDLEARDSDSEGGDDEREG
CCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
RAEAAPDVDDFRTMLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHHH
IERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQSI
HHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
ERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTA
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCH
EEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SDKHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHC
VL
CC
>Mature Secondary Structure 
PKSKRHEPQAESEKISRATQASVGRAGASTDDEDDVADNERDLEARDSDSEGGDDEREG
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCC
RAEAAPDVDDFRTMLQAELTADTIQHYLNRISVKPLLTVEEEQRYSRLAKAGEFEARQVM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECHHHHHHHHHHHCCCHHHHHHH
IERNLRLVVSIAKGYLNRGVPLLDLIEEGNLGLMHAIEKFDPTRGFRFSTYATWWIRQSI
HHHCHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHH
ERAIMNQARTVRLPVHVIRELNQVLRAKRHLEKNSMSTGEAAERREASIDDIAYLTGKTA
HHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHCCCCH
EEVTDILALNEHTASLDAPLDLDPASSLLDLLPDDQSQSPDAEVQHRELETLTRAWLSRL
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
SDKHRHVIERRFGLNHIEPATLEELADEMGLTRERVRQIQQEALVRLKRFFASNGVRKDA
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHC
VL
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]