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The map label for this gene is ghrB [H]

Identifier: 78066496

GI number: 78066496

Start: 2060767

End: 2061732

Strand: Reverse

Name: ghrB [H]

Synonym: Bcep18194_A5027

Alternate gene names: 78066496

Gene position: 2061732-2060767 (Counterclockwise)

Preceding gene: 78066497

Following gene: 78066495

Centisome position: 55.81

GC content: 70.08

Gene sequence:

>966_bases
ATGAAGCCCCGTATCGTCGCGTACAAGCCGCTGCCCGATGACGTCCTCGCGTACCTGCGCGAGCATGCGGACGTCGTGCA
GGCCGACGGCACCGCAGCCCTCGCCGACGCGCTCGGCGACGCGGACGGCGCGATCGGCGCGAGCCTGAAGGTCACGCCGC
AGATGCTCGACCGCGCCCCGCGGCTGAAGGCGTGGTCGACGATCTCGGTCGGCTACGACAACTTCGACGTCGCGGACCTC
ACGCGGCGCGGGATCGTGCTCGCCCATACGCCGGACGTGCTGACCGAGTCGACCGCCGATACGGTGTTCTCGCTGATCCT
CGCGTCCGCGCGACGCGTGGTCGAGCTGGCCGAGTGGGTGAAGGCCGGTAACTGGCATCACAGCATCGGCCCGGACCTGT
ACGGTACCGACGTGCAAGGCAAGACGCTCGGCATCGTCGGGCTCGGGCGGATCGGCGGCGCGGTCGCGCGCCGTGCGGCG
CTCGGCTTCCGGATGCAGGTGCTGTACGCGAACCGCTCCGCGCATGCGGAAGCCGAGACGCAGTACGGCGCGCGGCGCGT
CACGCTCGACGAACTGCTCGCGCAATCGGATTTCGTGTGCCTGCAGGTGCCGCTGTCGCCGGAAACGCACCACCTGATCG
GTGCGGCCGAATTCGCGAAGATGAAGCGCGGCGCGATCCTGATCAACGCATCGCGCGGGCCGGTGGTCGACGAAGCCGCG
CTGATCGATGCGCTGCGCGCGGGCACGATCCGCGGCGCGGGGCTCGACGTGTTCGAGAAGGAGCCGCTGGCGGCGGATTC
GCCGCTGCTGCAGATGAAGAATGTCGTCGCGCTGCCGCACATCGGCTCGGCGACGCACGAGACGCGCCATGCGATGGCGC
GCTGCGCTGCGGAAAACCTGGTCGGCGCGCTGGCCGGCACGCTGCGCACGAATCTCGTCAACCCGGATGCGCTCGCGCAG
GTCTGA

Upstream 100 bases:

>100_bases
GCGCCGCACTCGTCGCCGCGGTGATCCTCACGCTGTCCGTCAAACCCCAGGCGCGCGATACGCATCCGCTCGCGACGCCG
CTGCAAGGAAAATGAATCCG

Downstream 100 bases:

>100_bases
TCCGCCATATTCGTCCGGCCGGGGTGACGCCGGCGCTGTTAGAGGCGATGATCTTGGTGCCGGAGGGCTGGCTGCGTTAT
GATCCCCGCGCGTCGCGTCG

Product: gluconate 2-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKPRIVAYKPLPDDVLAYLREHADVVQADGTAALADALGDADGAIGASLKVTPQMLDRAPRLKAWSTISVGYDNFDVADL
TRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWVKAGNWHHSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAA
LGFRMQVLYANRSAHAEAETQYGARRVTLDELLAQSDFVCLQVPLSPETHHLIGAAEFAKMKRGAILINASRGPVVDEAA
LIDALRAGTIRGAGLDVFEKEPLAADSPLLQMKNVVALPHIGSATHETRHAMARCAAENLVGALAGTLRTNLVNPDALAQ
V

Sequences:

>Translated_321_residues
MKPRIVAYKPLPDDVLAYLREHADVVQADGTAALADALGDADGAIGASLKVTPQMLDRAPRLKAWSTISVGYDNFDVADL
TRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWVKAGNWHHSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAA
LGFRMQVLYANRSAHAEAETQYGARRVTLDELLAQSDFVCLQVPLSPETHHLIGAAEFAKMKRGAILINASRGPVVDEAA
LIDALRAGTIRGAGLDVFEKEPLAADSPLLQMKNVVALPHIGSATHETRHAMARCAAENLVGALAGTLRTNLVNPDALAQ
V
>Mature_321_residues
MKPRIVAYKPLPDDVLAYLREHADVVQADGTAALADALGDADGAIGASLKVTPQMLDRAPRLKAWSTISVGYDNFDVADL
TRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWVKAGNWHHSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAA
LGFRMQVLYANRSAHAEAETQYGARRVTLDELLAQSDFVCLQVPLSPETHHLIGAAEFAKMKRGAILINASRGPVVDEAA
LIDALRAGTIRGAGLDVFEKEPLAADSPLLQMKNVVALPHIGSATHETRHAMARCAAENLVGALAGTLRTNLVNPDALAQ
V

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=257, Percent_Identity=44.7470817120623, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI23308577, Length=285, Percent_Identity=35.0877192982456, Blast_Score=164, Evalue=9e-41,
Organism=Homo sapiens, GI145580578, Length=285, Percent_Identity=35.4385964912281, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI4557499, Length=285, Percent_Identity=35.4385964912281, Blast_Score=142, Evalue=3e-34,
Organism=Homo sapiens, GI61743967, Length=290, Percent_Identity=35.8620689655172, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI4557497, Length=290, Percent_Identity=35.8620689655172, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI145580575, Length=282, Percent_Identity=35.4609929078014, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI87082289, Length=320, Percent_Identity=59.375, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=34.8837209302326, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1787645, Length=191, Percent_Identity=31.9371727748691, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI87081824, Length=192, Percent_Identity=30.7291666666667, Blast_Score=82, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=307, Percent_Identity=31.9218241042345, Blast_Score=144, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI25147481, Length=283, Percent_Identity=29.3286219081272, Blast_Score=107, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6324055, Length=233, Percent_Identity=42.4892703862661, Blast_Score=176, Evalue=3e-45,
Organism=Saccharomyces cerevisiae, GI6320925, Length=262, Percent_Identity=35.8778625954198, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6322116, Length=262, Percent_Identity=35.1145038167939, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321253, Length=283, Percent_Identity=27.9151943462898, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6325144, Length=178, Percent_Identity=31.4606741573034, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6324964, Length=249, Percent_Identity=28.5140562248996, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324980, Length=179, Percent_Identity=30.7262569832402, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI45552429, Length=310, Percent_Identity=39.0322580645161, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45551003, Length=310, Percent_Identity=39.0322580645161, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574284, Length=310, Percent_Identity=39.0322580645161, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24585514, Length=310, Percent_Identity=39.0322580645161, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28574282, Length=310, Percent_Identity=39.0322580645161, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI28571528, Length=258, Percent_Identity=44.1860465116279, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI28574286, Length=311, Percent_Identity=36.0128617363344, Blast_Score=183, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24585516, Length=253, Percent_Identity=34.7826086956522, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI19921140, Length=298, Percent_Identity=34.8993288590604, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24646446, Length=298, Percent_Identity=36.241610738255, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646448, Length=298, Percent_Identity=36.241610738255, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646452, Length=298, Percent_Identity=36.241610738255, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24646450, Length=298, Percent_Identity=36.241610738255, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI62472511, Length=291, Percent_Identity=36.7697594501718, Blast_Score=126, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34078; Mature: 34078

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPRIVAYKPLPDDVLAYLREHADVVQADGTAALADALGDADGAIGASLKVTPQMLDRAP
CCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEECHHHHCCCC
RLKAWSTISVGYDNFDVADLTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWV
CCCCCEEEEECCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGNWHHSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAALGFRMQVLYANRSAHAEAET
HCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHH
QYGARRVTLDELLAQSDFVCLQVPLSPETHHLIGAAEFAKMKRGAILINASRGPVVDEAA
HHCCEEEEHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
LIDALRAGTIRGAGLDVFEKEPLAADSPLLQMKNVVALPHIGSATHETRHAMARCAAENL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHH
VGALAGTLRTNLVNPDALAQV
HHHHHHHHHHCCCCHHHHHCC
>Mature Secondary Structure
MKPRIVAYKPLPDDVLAYLREHADVVQADGTAALADALGDADGAIGASLKVTPQMLDRAP
CCCCEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCEEECHHHHCCCC
RLKAWSTISVGYDNFDVADLTRRGIVLAHTPDVLTESTADTVFSLILASARRVVELAEWV
CCCCCEEEEECCCCCCHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAGNWHHSIGPDLYGTDVQGKTLGIVGLGRIGGAVARRAALGFRMQVLYANRSAHAEAET
HCCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHCEEEEEEEECCCCCCHHHH
QYGARRVTLDELLAQSDFVCLQVPLSPETHHLIGAAEFAKMKRGAILINASRGPVVDEAA
HHCCEEEEHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCHHHH
LIDALRAGTIRGAGLDVFEKEPLAADSPLLQMKNVVALPHIGSATHETRHAMARCAAENL
HHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHCEEECCCCCCCHHHHHHHHHHHHHHHH
VGALAGTLRTNLVNPDALAQV
HHHHHHHHHHCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA