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The map label for this gene is nuoE2 [H]

Identifier: 78065618

GI number: 78065618

Start: 1080528

End: 1081028

Strand: Reverse

Name: nuoE2 [H]

Synonym: Bcep18194_A4146

Alternate gene names: 78065618

Gene position: 1081028-1080528 (Counterclockwise)

Preceding gene: 78065622

Following gene: 78065617

Centisome position: 29.26

GC content: 71.86

Gene sequence:

>501_bases
ATGTCCCCGAATTCCCACGCGCCCGACGCGCTCGTCGAGCGCCATGCACGCGCCGGCCGGTCGCTCGTGGCGATCCTGCA
CGCGATCCAGGACGACGCGGGTTACGTGCCGCCAGGCTGCGTCGCGCCGCTCGCCCGCGCACTCAACCTGTCGCGCGCGG
AAGTGCACGGCGTCCTGACTTACTACCACCACTTCCGCACCGCGCCGCCCGCGCGCGTGACGATCCAGATGTGTCGCGCC
GAAGCGTGCCGCAGCATGGGCTGCGAAGCGCTGGCCGCGCATGCGGAAGCCCGCACGGGCTGCCGGTTCGACGCGGCGCA
CGGCGATGCCGCCGATGCGCATGCGCCCGGTGACGTCGCACTCGAATCGGTCTACTGCCTCGGGCTGTGCGCGCAGTCGC
CGTCGCTAACGGTCAACGGCGTGCTGCATGCCAGGGTCACGCCGGAGAAATTCGACGCGCTGCTTGCCGAGGCCGCCGCC
CACACCCCGGAGGCCGCATGA

Upstream 100 bases:

>100_bases
AAATCTTCCGCTTATCGTTGGTCAAAATCAATCAGAACGAAGTCGCGATGCGCACTATATGAATCTCGAGCGCATATGAG
ACTTTGGAGGAGCACAACTG

Downstream 100 bases:

>100_bases
CGACCCGCATCTACGTCCCGCGCGACTCGTCCGCACTGGCGCTCGGCGCCGACGCGCTCGCCGCCGCGATTGCTGCGGAA
GCCGAACGGCGCGGCGTCGC

Product: NADH-ubiquinone oxidoreductase 24 kD subunit-like

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E 2; NDH-1 subunit E 2 [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSPNSHAPDALVERHARAGRSLVAILHAIQDDAGYVPPGCVAPLARALNLSRAEVHGVLTYYHHFRTAPPARVTIQMCRA
EACRSMGCEALAAHAEARTGCRFDAAHGDAADAHAPGDVALESVYCLGLCAQSPSLTVNGVLHARVTPEKFDALLAEAAA
HTPEAA

Sequences:

>Translated_166_residues
MSPNSHAPDALVERHARAGRSLVAILHAIQDDAGYVPPGCVAPLARALNLSRAEVHGVLTYYHHFRTAPPARVTIQMCRA
EACRSMGCEALAAHAEARTGCRFDAAHGDAADAHAPGDVALESVYCLGLCAQSPSLTVNGVLHARVTPEKFDALLAEAAA
HTPEAA
>Mature_165_residues
SPNSHAPDALVERHARAGRSLVAILHAIQDDAGYVPPGCVAPLARALNLSRAEVHGVLTYYHHFRTAPPARVTIQMCRAE
ACRSMGCEALAAHAEARTGCRFDAAHGDAADAHAPGDVALESVYCLGLCAQSPSLTVNGVLHARVTPEKFDALLAEAAAH
TPEAA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Escherichia coli, GI1788621, Length=133, Percent_Identity=27.0676691729323, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 17377; Mature: 17246

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPNSHAPDALVERHARAGRSLVAILHAIQDDAGYVPPGCVAPLARALNLSRAEVHGVLT
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
YYHHFRTAPPARVTIQMCRAEACRSMGCEALAAHAEARTGCRFDAAHGDAADAHAPGDVA
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHH
LESVYCLGLCAQSPSLTVNGVLHARVTPEKFDALLAEAAAHTPEAA
HHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SPNSHAPDALVERHARAGRSLVAILHAIQDDAGYVPPGCVAPLARALNLSRAEVHGVLT
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHH
YYHHFRTAPPARVTIQMCRAEACRSMGCEALAAHAEARTGCRFDAAHGDAADAHAPGDVA
HHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHH
LESVYCLGLCAQSPSLTVNGVLHARVTPEKFDALLAEAAAHTPEAA
HHHHHHHHHHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]