Click here to switch to the map view.

The map label for this gene is gatA [H]

Identifier: 78065606

GI number: 78065606

Start: 1065007

End: 1066383

Strand: Reverse

Name: gatA [H]

Synonym: Bcep18194_A4134

Alternate gene names: 78065606

Gene position: 1066383-1065007 (Counterclockwise)

Preceding gene: 78065607

Following gene: 78065605

Centisome position: 28.87

GC content: 73.28

Gene sequence:

>1377_bases
ATGACCACTTTCACGCCCTTCCCCCCGCTCGCCCAGCTCGCCGCCGACCTCGCCGCCGGCCGGACCACGAGCCGCGCACT
CGTCGATACCGCGCTCGACCGGATCGCCGATCCGTCGGGCCAGGGTGCGGTCGTCTTCACCGAAGTCGATGCCGACAACG
CGCGCGCGGCCGCCGACGCGCATGACCGGCTGCGCGCCGCCAGCACCGTGCTGTCGCCGCTCGCGGGCATTCCCGTGTCG
GTCAAGGACCTGTTCGACGTCGCGGGCCAGGTGACGCGCGCGGGTTCGCGCGTGCTCGACGGCGCGCCGGCCGCCCGCAC
CGATGCGGTCGCCGTCGCGCGGCTCAAGCGCGCGGGCGCGGTGCTGGTCGGCCGCACCAACATGAGCGAGTTCGCGTTCT
CGGGGCTCGGGCTGAATCCGCACTTCGGCCATCCGCGCTCGCCATACCACCGCGACGTGCCGGGCGACGCGCGGATCTCC
GGCGGCTCGTCGTCCGGCGCCGCCGCGTCCGTCGCCGACGGGATGGCCGCCGTCGCGCTCGGCACCGACACCGGCGGCTC
GATCCGCATTCCGGCCGCGCTGTGCGGGCTGACGGGCTTCAAGCCGACCGCCAGCCGGATCCCGACACAAGGCGGCGTGC
CGCTGTCGACGACGCTCGACTCGTTCGGCCCGATCGGCCTGACGGTCGCGTGCTGCGCGCTCGTCGACCGAATGCTCGCG
GGCCTCGAACCGCACGTGCCGGCCGCCCGGCCGCTCGAAGGCGTGCGGCTCGGCGTGCTGACGAACTATGTGACGGACGG
TGTCGATGCCGACGTCGCGGCCGCGCTCGACGCCGCGCTCAAGCATCTCGAAGCGGCCGGCGCGATCGTCACCGAAGTGC
GTTTCCCGGCGCTCGACCGGCTGCCGGACGTCAACCGCTTCGGCTTTTCGCCGATCGAGGCCTACGCGTGGCATCGCCCG
CTGCTCGCCAAGCACCGCGACCAGTACGACCCGCGCGTGCTCACGCGGATCCTGAAGGGCGAACCCGCGACCGCGGCCGA
CTATCTTGACCTGCTCGCCGCACGCGCGGCGATGCTCGACGAAGCCGCCCACACGGTCTGGGCGCGCTTCGATGCACTCG
TCGCGCCGACGGTGCCGGTCGTGCCGCCGCGCATCGCCGAACTCGAAACGGACGACGCCGCGTTCACACGCACCAACGCG
CTGATCCTGCGCAACCCGAGCGCGTTCAACTTCCTCGACGCGTGCGCGCTGTCGCTGCCCTGCCATCCGCGCGACGCGGC
GCCGGTCGGCCTGATGCTCGCGGCCGCACCGCATCGCGACGACGCGCTGCTCGCGATCGGCCAGGCGGTCGAGGCCGTGC
TGAACACGATCCGCTGA

Upstream 100 bases:

>100_bases
CTATCCGCGCGTGACGCCGCCGGCGCGCGCGCGGCCGTCGAAAGCCATATCGATTCGTTCCGCTCCACGCTTCTTCAACA
TCTTCGCCCCTGAGCCGCCC

Downstream 100 bases:

>100_bases
GCGCAGCCGAACGACCGCCGGCGGACAGGACGCGCGGTGCGGCAGGTTGCCGCACGCGCGCTGTCAGTTTCGTGCCAGAC
CGTTCGCGCTAAAATCGCGC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 458; Mature: 457

Protein sequence:

>458_residues
MTTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADAHDRLRAASTVLSPLAGIPVS
VKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHFGHPRSPYHRDVPGDARIS
GGSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDAALKHLEAAGAIVTEVRFPALDRLPDVNRFGFSPIEAYAWHRP
LLAKHRDQYDPRVLTRILKGEPATAADYLDLLAARAAMLDEAAHTVWARFDALVAPTVPVVPPRIAELETDDAAFTRTNA
LILRNPSAFNFLDACALSLPCHPRDAAPVGLMLAAAPHRDDALLAIGQAVEAVLNTIR

Sequences:

>Translated_458_residues
MTTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADAHDRLRAASTVLSPLAGIPVS
VKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHFGHPRSPYHRDVPGDARIS
GGSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDAALKHLEAAGAIVTEVRFPALDRLPDVNRFGFSPIEAYAWHRP
LLAKHRDQYDPRVLTRILKGEPATAADYLDLLAARAAMLDEAAHTVWARFDALVAPTVPVVPPRIAELETDDAAFTRTNA
LILRNPSAFNFLDACALSLPCHPRDAAPVGLMLAAAPHRDDALLAIGQAVEAVLNTIR
>Mature_457_residues
TTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADAHDRLRAASTVLSPLAGIPVSV
KDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGAVLVGRTNMSEFAFSGLGLNPHFGHPRSPYHRDVPGDARISG
GSSSGAAASVADGMAAVALGTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLAG
LEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDAALKHLEAAGAIVTEVRFPALDRLPDVNRFGFSPIEAYAWHRPL
LAKHRDQYDPRVLTRILKGEPATAADYLDLLAARAAMLDEAAHTVWARFDALVAPTVPVVPPRIAELETDDAAFTRTNAL
ILRNPSAFNFLDACALSLPCHPRDAAPVGLMLAAAPHRDDALLAIGQAVEAVLNTIR

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=316, Percent_Identity=30.379746835443, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI195972892, Length=493, Percent_Identity=24.1379310344828, Blast_Score=96, Evalue=8e-20,
Organism=Homo sapiens, GI166795287, Length=138, Percent_Identity=39.8550724637681, Blast_Score=76, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17543272, Length=443, Percent_Identity=26.1851015801354, Blast_Score=107, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=30.1242236024845, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17537465, Length=500, Percent_Identity=25, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71990152, Length=170, Percent_Identity=35.8823529411765, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556264, Length=161, Percent_Identity=36.6459627329193, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17538254, Length=321, Percent_Identity=27.1028037383178, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=387, Percent_Identity=28.9405684754522, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6323950, Length=432, Percent_Identity=25, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6320448, Length=169, Percent_Identity=30.7692307692308, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648113, Length=305, Percent_Identity=32.4590163934426, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI45550774, Length=146, Percent_Identity=37.6712328767123, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648435, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648437, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648441, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24648439, Length=146, Percent_Identity=37.6712328767123, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21356731, Length=207, Percent_Identity=33.8164251207729, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24644968, Length=492, Percent_Identity=23.5772357723577, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI161078093, Length=313, Percent_Identity=26.1980830670927, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24652985, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922090, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652981, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24652983, Length=158, Percent_Identity=31.0126582278481, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 47493; Mature: 47361

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADA
CCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHH
HDRLRAASTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHFGHPRSPYHRDVPGDARISGGSSSGAAASVADGMAAVAL
EEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHCCCEEEEE
GTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
ECCCCCCEECCHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDAALKHLEAAGAIVTEVRFPALDR
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
LPDVNRFGFSPIEAYAWHRPLLAKHRDQYDPRVLTRILKGEPATAADYLDLLAARAAMLD
CCCCCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAAHTVWARFDALVAPTVPVVPPRIAELETDDAAFTRTNALILRNPSAFNFLDACALSLP
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHEECCEEEEECCCHHHHHHHHHHCCC
CHPRDAAPVGLMLAAAPHRDDALLAIGQAVEAVLNTIR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTFTPFPPLAQLAADLAAGRTTSRALVDTALDRIADPSGQGAVVFTEVDADNARAAADA
CCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHH
HDRLRAASTVLSPLAGIPVSVKDLFDVAGQVTRAGSRVLDGAPAARTDAVAVARLKRAGA
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHCCE
VLVGRTNMSEFAFSGLGLNPHFGHPRSPYHRDVPGDARISGGSSSGAAASVADGMAAVAL
EEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHCCCEEEEE
GTDTGGSIRIPAALCGLTGFKPTASRIPTQGGVPLSTTLDSFGPIGLTVACCALVDRMLA
ECCCCCCEECCHHHHCCCCCCCHHHHCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHH
GLEPHVPAARPLEGVRLGVLTNYVTDGVDADVAAALDAALKHLEAAGAIVTEVRFPALDR
CCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHC
LPDVNRFGFSPIEAYAWHRPLLAKHRDQYDPRVLTRILKGEPATAADYLDLLAARAAMLD
CCCCCCCCCCHHHHHHHCCCHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
EAAHTVWARFDALVAPTVPVVPPRIAELETDDAAFTRTNALILRNPSAFNFLDACALSLP
HHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCCCHHEECCEEEEECCCHHHHHHHHHHCCC
CHPRDAAPVGLMLAAAPHRDDALLAIGQAVEAVLNTIR
CCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA