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The map label for this gene is greB

Identifier: 78065582

GI number: 78065582

Start: 1039891

End: 1040454

Strand: Direct

Name: greB

Synonym: Bcep18194_A4110

Alternate gene names: 78065582

Gene position: 1039891-1040454 (Clockwise)

Preceding gene: 78065581

Following gene: 78065585

Centisome position: 28.15

GC content: 63.83

Gene sequence:

>564_bases
ATGAACAAGGCGTTTGTCAAAGAGTCGGACGGCGACGACGACGATCTGGACCAGGCCCAGCCGGCGATTCCGGCGGGCTC
GAAGAACTACATCACACCGGCTGGTCACAAGCGGCTGAGGGACGAACTGCTGAACCTGATCGACGTCGAGCGTCCCGAGG
TCGTGCGGCTCGTGTCCTGGGCCGCGTCGAACGGCGACCGGTCGGAGAACGGCGACTATATCTACGGCAAGCGGCGGCTT
CGCGAGATCGATCGCCGCATCCGCTTCCTGACGAAGCGGCTCGATCTGGCCGAAGTCGTCGATGCGAGCCGGCAGGAGAA
CGTCGACCAGGTGTTTTTCGGTGCAACGGTCGATTACGAAACGCCGGACGGCGAGGATCACACGATCACGATCGTCGGGA
TCGACGAGGTCGATCTCGATCACGGTTGTGTCAGCTGGATTTCGCCGGTCGCGCGGGCGCTGATCAAGGCGAAGATCGGC
GATACCGTCATGCTGATGACGCCGGCCGGCCCGCAGCCGATCGACGTGCTCGACGTCCGTTACCCGGCGTCGGGCAACGC
CTGA

Upstream 100 bases:

>100_bases
TATAAAAAGGGAAGCCTTAATCGCAAATTAGGTGCATGGCCTTGCCGGGGCGCCCTTCGTGTACCCTTGGAGCATCAGTC
ATCCATGTTGAACCCGCATC

Downstream 100 bases:

>100_bases
GCGGCGCTCGACGCAGCCCGGCAAAGAAAAAGCGCCCCGATGGGGCGCTTCGTCTTCCGGCGTGCAGCCGATGTGCCTGT
TGTTCAGAAGCGGTGGCGCA

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNKAFVKESDGDDDDLDQAQPAIPAGSKNYITPAGHKRLRDELLNLIDVERPEVVRLVSWAASNGDRSENGDYIYGKRRL
REIDRRIRFLTKRLDLAEVVDASRQENVDQVFFGATVDYETPDGEDHTITIVGIDEVDLDHGCVSWISPVARALIKAKIG
DTVMLMTPAGPQPIDVLDVRYPASGNA

Sequences:

>Translated_187_residues
MNKAFVKESDGDDDDLDQAQPAIPAGSKNYITPAGHKRLRDELLNLIDVERPEVVRLVSWAASNGDRSENGDYIYGKRRL
REIDRRIRFLTKRLDLAEVVDASRQENVDQVFFGATVDYETPDGEDHTITIVGIDEVDLDHGCVSWISPVARALIKAKIG
DTVMLMTPAGPQPIDVLDVRYPASGNA
>Mature_187_residues
MNKAFVKESDGDDDDLDQAQPAIPAGSKNYITPAGHKRLRDELLNLIDVERPEVVRLVSWAASNGDRSENGDYIYGKRRL
REIDRRIRFLTKRLDLAEVVDASRQENVDQVFFGATVDYETPDGEDHTITIVGIDEVDLDHGCVSWISPVARALIKAKIG
DTVMLMTPAGPQPIDVLDVRYPASGNA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=151, Percent_Identity=49.0066225165563, Blast_Score=135, Evalue=2e-33,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=35.5263157894737, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20761; Mature: 20761

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2 ; PS00289 PENTAXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAFVKESDGDDDDLDQAQPAIPAGSKNYITPAGHKRLRDELLNLIDVERPEVVRLVSW
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDLAEVVDASRQENVDQVFFGATVDYE
HCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCEECCC
TPDGEDHTITIVGIDEVDLDHGCVSWISPVARALIKAKIGDTVMLMTPAGPQPIDVLDVR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEE
YPASGNA
CCCCCCC
>Mature Secondary Structure
MNKAFVKESDGDDDDLDQAQPAIPAGSKNYITPAGHKRLRDELLNLIDVERPEVVRLVSW
CCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
AASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDLAEVVDASRQENVDQVFFGATVDYE
HCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCEECCC
TPDGEDHTITIVGIDEVDLDHGCVSWISPVARALIKAKIGDTVMLMTPAGPQPIDVLDVR
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEE
YPASGNA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]