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The map label for this gene is spoT [H]

Identifier: 78065581

GI number: 78065581

Start: 1036845

End: 1039211

Strand: Direct

Name: spoT [H]

Synonym: Bcep18194_A4109

Alternate gene names: 78065581

Gene position: 1036845-1039211 (Clockwise)

Preceding gene: 78065580

Following gene: 78065582

Centisome position: 28.07

GC content: 64.85

Gene sequence:

>2367_bases
ATGAGCACCACCCCATCGTCCGCCTCCGCGGATTCGACCACCGAAGCCACGGCCCAGTCGCCTGCGCGCCAGTACATCGA
CGCGGTCCTCGAACAGTCCTTCCGGCATCTGTTCGGGCCGACCGCGACACCGGAGCAGCCTCGCAAGCACGGCGTCGTTT
CGATCGCGAAACTGACGGCCGCGCTTGCCGAGTATCTTCCTCCGGAAGAAATCAAAGAGGTCAAGGCGGCATTCCACTTC
AGCGACGAAGCCCACCTCGGTCAATATCGCCAGAGCGGCGAGCCCTACATCACCCATCCTGTCGCCGTTGCGGAAATCTG
CGCCGGCTGGAAGCTCGACGCGCAGGCCGTGATGGCAGCGCTCCTGCACGACGTGATGGAAGATCAGGGCGTGACCAAGA
GCGAGCTGGCCGAACGGTTCGGCCCGAAGGTCGCCGAACTGGTCGACGGCCTGTCGAAGCTCGACAAGATGGAGTTCCGC
AGCCGCGAGGAAGCGCAGGCGGAAAACTTCCGCAAGATGCTGCTCGCGATGGCACGCGACGTGCGCGTCATTCTCGTCAA
GCTCGCCGACCGCCTGCACAACATGCGCACGCTCGGCGCGGTGCCGATGGAAAAGCGCCGCCGCGTCGCGCGGGAAACGC
TCGACATCTACGCGCCGATCGCGCACCGCCTCGGGTTGAACAACACGTATCGCGAGCTGCAGGACATGAGCTTCGCGAAC
TTCAACCCGCATCGCTACGCGACGCTCGAGAAGGCCGTGAAGGCCGCGCGCGGCAATCGCCGCGAAGTGATCAGCAAGAT
CCTCGAGGCCGCGCAGCGCGCGATGGCCGACGCGAAGATCGACGCCGAGATCACCGGCCGCGAAAAGACCATCTACAGCG
TGTACCGCAAGATGCGCGACAAGCAGCTGTCGTTCTCGCAGGTGCTCGACGTGTACGGCTTTCGCGTCGTCGTCGACAGC
CCGCTCGACTGCTACACGTGCATCGGCGCGCTGCACGCGCTGTACAAGCCCGTGCCCGGCAAGTTCAAGGACTACATCGC
GATCCCGAAGATCAACGGCTATCAGTCGCTGCACACGACGCTCGTCGGCCCGTTCGGCGCGCCGATCGAGTTCCAGGTGC
GCACGCGCAAGATGCACGAGATCGCCGAGGCGGGGGGCGCCGCGCACTGGCTGTACAAGAACGGCAGCGCGGATCTCAGC
GACGTGCAGAAGCGCGCGCACCAGTGGCTGAAGTCGCTGCTCGACATCCAGAGCGAAGCCGGCGATTCGAGCGAATTCCT
CGAGCACGTGAAGATCGACCTGTTCCCGGATGCGGTCTACGTGTTCACGCCGAAGTCGAAGATCATGGCGCTGCCGCGCG
GCGCGACGGCACTCGATTTCGCGTATTCGATCCACAGCGATCTCGGCAACCAGTGCGTGGCCGTGAAGATCAACAACGAA
CTGCTGCCGCTGCGCACCGAGCTGAAGAGCGGCGACATCGTCGAGGTGATCACCGCGCCGTATTCGAAGCCGAACCCAGC
GTGGCTCGGCTTCGTGCGTACCGGCAAGGCGCGCTCGGCGATCCGGCACTACCTGAAGACGATGCGCCTGAACGAATCGG
TGCAGCTTGGCGAGCGGCTGGTCGACCAGAGCCTGAAGGGCTACGGTCTTGCGCTGGCCGATGTCGAGCCGGAAGTGTGG
GAGAAGCTCGTGCAGTGGACGGGCAACAAGAGCCGTCAGGAAATCTTCGCGGACATCGGCCTCGGCCGTCGCGTGGCCGC
GGTGATGGCCAAGCGCATCGAGGTGCTGATGAGCGGCCGCGATGCGGACGACGATCTGCCGAAGTCCGAGCGGCATCCCG
CGCACCATGCGCCGCCGGTCGTGATTACCGGCACCGAAGGGATGTCCGTGCAGTTGTCCGCGTGCTGCCGGCCGATTCCG
GGCGACGCGATCATGGGCTATATCGGCATTGGCCTGGGGATGGCGATTCATACGACCGATTGCCGCGTGGCGCAGCGTAT
TCATCGCCGCGATCCGGGCCGCTGGATCGATGTCGAATGGGCGCCGCAGCCGGGCCGCCTGTTCGATGTCGCCGTGAAGG
CGCTCGTGAAGAACACGAAGGGCATCTTTGCGCGCGTGGCGGCGGATATCACGTCGGCCGATGCGAACATCGTGCACATT
GCGATGGACGAGGATCTGACGCACGAATCGACGGTGCTGCGCTTCGTGATCCAGGTCAGCGATCGCGTGCATCTCGCGAA
CGTGATGCGGCGCGTGCGCACCAATCCGGATGTGATGCGGATCATGCGCGAGCGTTCGACCGACGACGGCGCGCATGCGC
GCCACGACGGTGGCATGCGCATCGAGCGCGAGCGGCAGGATTATTGA

Upstream 100 bases:

>100_bases
GGTCGGCGTCGAGATGCTGAAGAAGGTGCCGGTGTAACGCGCATCCGGCCCGTTCCAGCCATGTAATCCACGCGCGCAAC
GACTCGACCTGGAGGCGAAT

Downstream 100 bases:

>100_bases
CGCTACGCCCGCGTGGGCGATCGATCGACGGCGGCCAATGGCCGCCGTTTTCATTTGTGCCGCAGGGGAGGCGAACGTGC
GGCGTGGCCGACGTTCTTCC

Product: (p)ppGpp synthetase I, SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MSTTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPEEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGGAAHWLYKNGSADLS
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVEPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMRIERERQDY

Sequences:

>Translated_788_residues
MSTTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPEEIKEVKAAFHF
SDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFR
SREEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRDKQLSFSQVLDVYGFRVVVDS
PLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGGAAHWLYKNGSADLS
DVQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVEPEVW
EKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIP
GDAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMRIERERQDY
>Mature_787_residues
STTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTAALAEYLPPEEIKEVKAAFHFS
DEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAALLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRS
REEAQAENFRKMLLAMARDVRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFANF
NPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRDKQLSFSQVLDVYGFRVVVDSP
LDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTTLVGPFGAPIEFQVRTRKMHEIAEAGGAAHWLYKNGSADLSD
VQKRAHQWLKSLLDIQSEAGDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNEL
LPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERLVDQSLKGYGLALADVEPEVWE
KLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGRDADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIPG
DAIMGYIGIGLGMAIHTTDCRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHIA
MDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMRIERERQDY

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=710, Percent_Identity=45.3521126760563, Blast_Score=660, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=683, Percent_Identity=31.7715959004392, Blast_Score=384, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 87955; Mature: 87824

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHH
ALAEYLPPEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAA
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGGAAHWLYKNGSADLSDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLALADVEPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGR
HHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCE
IERERQDY
ECHHCCCC
>Mature Secondary Structure 
STTPSSASADSTTEATAQSPARQYIDAVLEQSFRHLFGPTATPEQPRKHGVVSIAKLTA
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCHHHHHHHHH
ALAEYLPPEEIKEVKAAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDAQAVMAA
HHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHH
LLHDVMEDQGVTKSELAERFGPKVAELVDGLSKLDKMEFRSREEAQAENFRKMLLAMARD
HHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VRVILVKLADRLHNMRTLGAVPMEKRRRVARETLDIYAPIAHRLGLNNTYRELQDMSFAN
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCC
FNPHRYATLEKAVKAARGNRREVISKILEAAQRAMADAKIDAEITGREKTIYSVYRKMRD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHHHH
KQLSFSQVLDVYGFRVVVDSPLDCYTCIGALHALYKPVPGKFKDYIAIPKINGYQSLHTT
HCCCHHHHHHHHCCEEEECCCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHH
LVGPFGAPIEFQVRTRKMHEIAEAGGAAHWLYKNGSADLSDVQKRAHQWLKSLLDIQSEA
HCCCCCCCEEEEHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
GDSSEFLEHVKIDLFPDAVYVFTPKSKIMALPRGATALDFAYSIHSDLGNQCVAVKINNE
CCHHHHHHHHHHEECCCEEEEECCCHHEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCC
LLPLRTELKSGDIVEVITAPYSKPNPAWLGFVRTGKARSAIRHYLKTMRLNESVQLGERL
EECHHHHCCCCCEEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCHHHHHHHH
VDQSLKGYGLALADVEPEVWEKLVQWTGNKSRQEIFADIGLGRRVAAVMAKRIEVLMSGR
HHHHHCCCCEEEECCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DADDDLPKSERHPAHHAPPVVITGTEGMSVQLSACCRPIPGDAIMGYIGIGLGMAIHTTD
CCCCCCCCCCCCCCCCCCCEEEECCCCCEEEHHHCCCCCCCHHHHHHHHHHHCCEEECCH
CRVAQRIHRRDPGRWIDVEWAPQPGRLFDVAVKALVKNTKGIFARVAADITSADANIVHI
HHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
AMDEDLTHESTVLRFVIQVSDRVHLANVMRRVRTNPDVMRIMRERSTDDGAHARHDGGMR
EECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCE
IERERQDY
ECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]