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The map label for this gene is ribH

Identifier: 78065529

GI number: 78065529

Start: 980276

End: 980794

Strand: Reverse

Name: ribH

Synonym: Bcep18194_A4057

Alternate gene names: 78065529

Gene position: 980794-980276 (Counterclockwise)

Preceding gene: 78065530

Following gene: 78065528

Centisome position: 26.55

GC content: 64.93

Gene sequence:

>519_bases
ATGGAAATCGGACAATACCAACCGAATCTCGAAGGCGACGGCCTGCGTATCGGCATCGTGCAATCGCGCTTCAACGAGCC
CGTGTGCAACGGCCTCGCCGACGCGTGCGTCGAGGAACTGGAACGCCTCGGCGTCACCGGTGAAGACGTGCTGCTCGTGT
CGGTGCCCGGCGCGCTGGAAATCCCGCTCGCGCTGCAGAAGCTCGCGGAAAGCGGCCAGTTCGACGCGCTGATCGCGCTC
GGCGCAGTGATCCGCGGCGAGACCTACCACTTCGAACTCGTGTCGAACGAGAGCGGCGCGGGCATCACCCGCATCGGCCT
CGACTTCAACCTGCCGATCGCGAACGCGGTCCTGACGACCGAAAACGACGAGCAGGCCGTCGCGCGCATGACCGAAAAGG
GTCGTGACGCCGCACGCGTCGCCGTCGAGATGGCCAACCTGACGATGGCACTCGACCAGCTCGGCGATGACGACGAGGAC
GAAGAGGAAGACGAAGACGACGAAGAGGAGCGCGCATGA

Upstream 100 bases:

>100_bases
CGATGCCCGGCGGCGAAGCCAAGTCCTGCCCGGCGTCCAACGCGTAACCCGCCACGGCGCTTTCGCCCTATAACCGTTCA
TCCACACCACGGACAAGATC

Downstream 100 bases:

>100_bases
AGAAGAGCGCCCGCCGACAATCGCGCGAGCTGGCGACGCAGGGGCTGTACCAGTGGCTGCTGTCGAACGCGTCCTCCGGT
GAGATCGACGCGCAACTGCG

Product: 6,7-dimethyl-8-ribityllumazine synthase

Products: NA

Alternate protein names: DMRL synthase; Lumazine synthase; Riboflavin synthase beta chain [H]

Number of amino acids: Translated: 172; Mature: 172

Protein sequence:

>172_residues
MEIGQYQPNLEGDGLRIGIVQSRFNEPVCNGLADACVEELERLGVTGEDVLLVSVPGALEIPLALQKLAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRIGLDFNLPIANAVLTTENDEQAVARMTEKGRDAARVAVEMANLTMALDQLGDDDED
EEEDEDDEEERA

Sequences:

>Translated_172_residues
MEIGQYQPNLEGDGLRIGIVQSRFNEPVCNGLADACVEELERLGVTGEDVLLVSVPGALEIPLALQKLAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRIGLDFNLPIANAVLTTENDEQAVARMTEKGRDAARVAVEMANLTMALDQLGDDDED
EEEDEDDEEERA
>Mature_172_residues
MEIGQYQPNLEGDGLRIGIVQSRFNEPVCNGLADACVEELERLGVTGEDVLLVSVPGALEIPLALQKLAESGQFDALIAL
GAVIRGETYHFELVSNESGAGITRIGLDFNLPIANAVLTTENDEQAVARMTEKGRDAARVAVEMANLTMALDQLGDDDED
EEEDEDDEEERA

Specific function: Riboflavin synthase is a bifunctional enzyme complex catalyzing the formation of riboflavin from 5-amino-6-(1'-D)- ribityl-amino-2,4(1H,3H)-pyrimidinedione and L-3,4-dihydrohy-2- butanone-4-phosphate via 6,7-dimethyl-8-lumazine. The beta subunit catalyzes

COG id: COG0054

COG function: function code H; Riboflavin synthase beta-chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DMRL synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786617, Length=142, Percent_Identity=42.2535211267606, Blast_Score=108, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324429, Length=148, Percent_Identity=29.7297297297297, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002180 [H]

Pfam domain/function: PF00885 DMRL_synthase [H]

EC number: =2.5.1.9 [H]

Molecular weight: Translated: 18561; Mature: 18561

Theoretical pI: Translated: 3.69; Mature: 3.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIGQYQPNLEGDGLRIGIVQSRFNEPVCNGLADACVEELERLGVTGEDVLLVSVPGALE
CCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
IPLALQKLAESGQFDALIALGAVIRGETYHFELVSNESGAGITRIGLDFNLPIANAVLTT
HHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCEEEEC
ENDEQAVARMTEKGRDAARVAVEMANLTMALDQLGDDDEDEEEDEDDEEERA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure
MEIGQYQPNLEGDGLRIGIVQSRFNEPVCNGLADACVEELERLGVTGEDVLLVSVPGALE
CCCCCCCCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCC
IPLALQKLAESGQFDALIALGAVIRGETYHFELVSNESGAGITRIGLDFNLPIANAVLTT
HHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCEEEECCEECCCCCCEEEEC
ENDEQAVARMTEKGRDAARVAVEMANLTMALDQLGDDDEDEEEDEDDEEERA
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA