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The map label for this gene is nac [H]

Identifier: 78065509

GI number: 78065509

Start: 962081

End: 963010

Strand: Reverse

Name: nac [H]

Synonym: Bcep18194_A4037

Alternate gene names: 78065509

Gene position: 963010-962081 (Counterclockwise)

Preceding gene: 78065513

Following gene: 78065508

Centisome position: 26.07

GC content: 69.68

Gene sequence:

>930_bases
ATGGACTTGAGGCAGTTGCGCTATTTCGTGAAGGTCGTCGAATGCGGCAACGTCACGCATGCGAGCGAGGCGCTGCATGT
CGCGCAGCCGGCGGTCAGCCAGCAGATGCGCAATCTCGAACAGGATCTCGGGATGCAGTTGCTCGAACGCAGCGTGCGCG
GCGTTGCGCCCACCGCCGCCGGCCGCACGCTGTACCAGCACGCGCTCGAGCTGCTGCGCCAGGCCGACGGCACGCGCGAG
TTGCTGCGCCGCGACGCCGACACACCGCAGGGCCGCGTGACGGTCGGGATGCCGTCGAGCACCGCGCGCGTGCTGGCCAT
TCCGCTCGCCCGCGCGGTGCGCGACCGCTATCCGGGCATCATGCTGGAGCTGATCGAGGCGCCGAGCGCCGATCTCGACA
CGTTGCTCGAACGCACGCGGCTCGATCTCGCGATCGTCGTCGACGCGGTCGACACGCGCGGCATCGCGATCCACCGGCTG
CTGACGGAAACGCTGTACCTGATCACGTGGCCCGGGTTCCAGGTGCCGGGCGATCCCGTGCCGCTCGACGCGATCGCGCA
GATGCCGCTCGTACTGCCGAGCGCGCCGAACACGATCCGCAACCGCGTCGACTGGGCGATGCGCGAGGCCGGGCTGTCGT
ACGAGATCAGCTTCGAGGCGAGCTCGACCGGGCTGCTGTTCGCGGCCGTGATGGCGCAGCTCGGCGTGACGATCCTGCCG
TGGACGGCCGCGCACGTCGAGATCGAGGAGCGCAAGCTCAAGCTGTCGACGGTCGCGCATCGGCTGTTCGCGCGCGACCT
GTCGCTGTGCTGGCACGATACGGCGCTCGTCAGCAACGCGGTGCAGAAAGTGAAAGCCGAGATCGTCCGGCTGTTCGACA
CGCTCGGGCAGCGGCCCGAGTGGGCGGCCGGGCAGGCCGGCCGTGCGTGA

Upstream 100 bases:

>100_bases
TTTGTGAGACGAGTATAGAAACACCATCGATCGGAGAAAAATATGATATTCATCTTGCTCGATCGGATTGGCTTATGGCT
CGAATCGGGAGGCGGCGGCG

Downstream 100 bases:

>100_bases
CCGGGGCGCCGCGTGCCTTGCCCCTTCCTTGTGCTGCGAAGGCGCGCGGACTAAGCTTCGTTCATCCTGGGTACCGCTGA
CACCCCCACTTCTCCTCACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAAGRTLYQHALELLRQADGTRE
LLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGIMLELIEAPSADLDTLLERTRLDLAIVVDAVDTRGIAIHRL
LTETLYLITWPGFQVPGDPVPLDAIAQMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKAEIVRLFDTLGQRPEWAAGQAGRA

Sequences:

>Translated_309_residues
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAAGRTLYQHALELLRQADGTRE
LLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGIMLELIEAPSADLDTLLERTRLDLAIVVDAVDTRGIAIHRL
LTETLYLITWPGFQVPGDPVPLDAIAQMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKAEIVRLFDTLGQRPEWAAGQAGRA
>Mature_309_residues
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAAGRTLYQHALELLRQADGTRE
LLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGIMLELIEAPSADLDTLLERTRLDLAIVVDAVDTRGIAIHRL
LTETLYLITWPGFQVPGDPVPLDAIAQMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKAEIVRLFDTLGQRPEWAAGQAGRA

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=246, Percent_Identity=40.650406504065, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI157672245, Length=238, Percent_Identity=31.5126050420168, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1790399, Length=244, Percent_Identity=27.4590163934426, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788887, Length=171, Percent_Identity=35.0877192982456, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787530, Length=254, Percent_Identity=26.7716535433071, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788481, Length=283, Percent_Identity=26.1484098939929, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787601, Length=179, Percent_Identity=28.4916201117318, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=262, Percent_Identity=27.4809160305344, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788296, Length=254, Percent_Identity=26.7716535433071, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788748, Length=240, Percent_Identity=28.3333333333333, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1789639, Length=159, Percent_Identity=31.4465408805031, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786401, Length=225, Percent_Identity=26.2222222222222, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=26.3473053892216, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787806, Length=177, Percent_Identity=29.9435028248588, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI145693193, Length=125, Percent_Identity=32.8, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082132, Length=255, Percent_Identity=25.8823529411765, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788626, Length=247, Percent_Identity=28.7449392712551, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789173, Length=263, Percent_Identity=28.8973384030418, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1787589, Length=251, Percent_Identity=26.2948207171315, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI2367136, Length=240, Percent_Identity=25.8333333333333, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34089; Mature: 34089

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GRTLYQHALELLRQADGTRELLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGI
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
MLELIEAPSADLDTLLERTRLDLAIVVDAVDTRGIAIHRLLTETLYLITWPGFQVPGDPV
HHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCC
PLDAIAQMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
CHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEC
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKAEIVRLFDTLGQRPE
CCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WAAGQAGRA
CCCCCCCCC
>Mature Secondary Structure
MDLRQLRYFVKVVECGNVTHASEALHVAQPAVSQQMRNLEQDLGMQLLERSVRGVAPTAA
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GRTLYQHALELLRQADGTRELLRRDADTPQGRVTVGMPSSTARVLAIPLARAVRDRYPGI
HHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCH
MLELIEAPSADLDTLLERTRLDLAIVVDAVDTRGIAIHRLLTETLYLITWPGFQVPGDPV
HHHHHHCCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHEEEECCCCCCCCCCC
PLDAIAQMPLVLPSAPNTIRNRVDWAMREAGLSYEISFEASSTGLLFAAVMAQLGVTILP
CHHHHHHCCEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCCEEEC
WTAAHVEIEERKLKLSTVAHRLFARDLSLCWHDTALVSNAVQKVKAEIVRLFDTLGQRPE
CCHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
WAAGQAGRA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]