Click here to switch to the map view.

The map label for this gene is rfbD [H]

Identifier: 78065446

GI number: 78065446

Start: 892957

End: 893841

Strand: Direct

Name: rfbD [H]

Synonym: Bcep18194_A3972

Alternate gene names: 78065446

Gene position: 892957-893841 (Clockwise)

Preceding gene: 78065445

Following gene: 78065455

Centisome position: 24.17

GC content: 63.05

Gene sequence:

>885_bases
ATGAATTCCGAGTCGACGATCCTCGTAACCGGTGTCACGGGTCAGGTAGGCTTTGAACTGCTGCGCTCGCTGCAGGGGCT
CGGCCGTGTGGTGGAGTGCGACCGCTCGATGCTCGATCTGTCTGATCTCGACCGGATCCGTGCTGTCGTGCGGGCGTTGC
AGCCGGCCTTCATCGTGAATCCGGCTGCCTATACGGCCGTTGATAATGCCGAGGACGACGTCGATGCGGCAAGACGCATA
AATGCCGATGTGCCGCGGGTCCTGGCGGAAGAGGCTGCGCGCAGCGGTGCGGTGCTGATTCACTACTCGACCGATTACGT
GTTCGACGGAGCCAAGGCGGGCGCGTATACCGAGACCGATGTAGTGAATCCGCTGAACGTGTATGGGATGACCAAGCTTG
AGGGCGAGCGTGCGATCGAGAACGCAGGGTGCGCGTACCTGACCTTGCGAACTAGCTGGGTATACGGGCGACGTGGCAGG
AATTTCTTGCGTACGATGCTCAAGCTGGGCGCGGAGCGACCCGAATTGCGTGTGGTTGCCGACCAGGTCGGTGCGCCGAC
CTGGTCGAAAACAATTGCAGCCGCGACTGCGCACATCCTCTCCAAAGGGCTGGCGGCCCATGATGAGGCCTGGTGGCAGG
CGCGCTCCGGAACCTATCATCTGAGTGCGGCCGGCGAGACGTCCTGGCACGGTTTCGCCAAGGCGATTCTCGGCATCGCG
ATGGGGGATGACGCACCCGCTGTGGTGCCGATTTCCGCGAGCGAGTTTCCGGTGCGCGCACGTCGCCCGGCGAATTCGCG
CTTGTCCCACGGCAAACTCGCCGAGACGTTCGGCTTGCACCTGCCCGATTGGGAATACGCGCTGGGCCTCTGCATGGCCG
ACTGA

Upstream 100 bases:

>100_bases
ATCGGAATCGTGTGGCCGCTGGATGGCGAGCCGCTGCTGGCCGTCAAGGATGCCGCGGGCATGCGGTTGATGGACGCTGA
TCTTTTTGAGTGAGGTCGTG

Downstream 100 bases:

>100_bases
AACGGATCGCCGGTGGTCGAACCCCTGCATCGCCAGGTATTGACCGGGCGCGTCAGGGTAACGGGCAGTCGAGCAAGCGG
CGGATCCCGTCACCGAGTTT

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MNSESTILVTGVTGQVGFELLRSLQGLGRVVECDRSMLDLSDLDRIRAVVRALQPAFIVNPAAYTAVDNAEDDVDAARRI
NADVPRVLAEEAARSGAVLIHYSTDYVFDGAKAGAYTETDVVNPLNVYGMTKLEGERAIENAGCAYLTLRTSWVYGRRGR
NFLRTMLKLGAERPELRVVADQVGAPTWSKTIAAATAHILSKGLAAHDEAWWQARSGTYHLSAAGETSWHGFAKAILGIA
MGDDAPAVVPISASEFPVRARRPANSRLSHGKLAETFGLHLPDWEYALGLCMAD

Sequences:

>Translated_294_residues
MNSESTILVTGVTGQVGFELLRSLQGLGRVVECDRSMLDLSDLDRIRAVVRALQPAFIVNPAAYTAVDNAEDDVDAARRI
NADVPRVLAEEAARSGAVLIHYSTDYVFDGAKAGAYTETDVVNPLNVYGMTKLEGERAIENAGCAYLTLRTSWVYGRRGR
NFLRTMLKLGAERPELRVVADQVGAPTWSKTIAAATAHILSKGLAAHDEAWWQARSGTYHLSAAGETSWHGFAKAILGIA
MGDDAPAVVPISASEFPVRARRPANSRLSHGKLAETFGLHLPDWEYALGLCMAD
>Mature_294_residues
MNSESTILVTGVTGQVGFELLRSLQGLGRVVECDRSMLDLSDLDRIRAVVRALQPAFIVNPAAYTAVDNAEDDVDAARRI
NADVPRVLAEEAARSGAVLIHYSTDYVFDGAKAGAYTETDVVNPLNVYGMTKLEGERAIENAGCAYLTLRTSWVYGRRGR
NFLRTMLKLGAERPELRVVADQVGAPTWSKTIAAATAHILSKGLAAHDEAWWQARSGTYHLSAAGETSWHGFAKAILGIA
MGDDAPAVVPISASEFPVRARRPANSRLSHGKLAETFGLHLPDWEYALGLCMAD

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=286, Percent_Identity=31.8181818181818, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI11034825, Length=286, Percent_Identity=31.8181818181818, Blast_Score=99, Evalue=3e-21,
Organism=Escherichia coli, GI1788352, Length=289, Percent_Identity=43.598615916955, Blast_Score=219, Evalue=2e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 31784; Mature: 31784

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSESTILVTGVTGQVGFELLRSLQGLGRVVECDRSMLDLSDLDRIRAVVRALQPAFIVN
CCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEC
PAAYTAVDNAEDDVDAARRINADVPRVLAEEAARSGAVLIHYSTDYVFDGAKAGAYTETD
CHHHEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCC
VVNPLNVYGMTKLEGERAIENAGCAYLTLRTSWVYGRRGRNFLRTMLKLGAERPELRVVA
CCCCHHHCEEEEECCHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEH
DQVGAPTWSKTIAAATAHILSKGLAAHDEAWWQARSGTYHLSAAGETSWHGFAKAILGIA
HHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHE
MGDDAPAVVPISASEFPVRARRPANSRLSHGKLAETFGLHLPDWEYALGLCMAD
ECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHEEECC
>Mature Secondary Structure
MNSESTILVTGVTGQVGFELLRSLQGLGRVVECDRSMLDLSDLDRIRAVVRALQPAFIVN
CCCCCEEEEEECCHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHHHCCCEEEC
PAAYTAVDNAEDDVDAARRINADVPRVLAEEAARSGAVLIHYSTDYVFDGAKAGAYTETD
CHHHEECCCCCHHHHHHHHCCCCHHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCCCCC
VVNPLNVYGMTKLEGERAIENAGCAYLTLRTSWVYGRRGRNFLRTMLKLGAERPELRVVA
CCCCHHHCEEEEECCHHHHHCCCEEEEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEH
DQVGAPTWSKTIAAATAHILSKGLAAHDEAWWQARSGTYHLSAAGETSWHGFAKAILGIA
HHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHE
MGDDAPAVVPISASEFPVRARRPANSRLSHGKLAETFGLHLPDWEYALGLCMAD
ECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]