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The map label for this gene is rfbB [H]

Identifier: 78065443

GI number: 78065443

Start: 890447

End: 891508

Strand: Direct

Name: rfbB [H]

Synonym: Bcep18194_A3969

Alternate gene names: 78065443

Gene position: 890447-891508 (Clockwise)

Preceding gene: 78065441

Following gene: 78065444

Centisome position: 24.1

GC content: 62.62

Gene sequence:

>1062_bases
ATGATTCTGGTGACGGGCGGCGCGGGTTTTATCGGCGCCAATTTCGTAATCGACTGGCTGTGTCAATCCGACGAGGCCGT
GCTCAATGTCGATAAATTGACCTATGCAGGCAACCTTCGCACGCTGCAATCGCTGAACGGAAGCCCGAAGCACGTATTTG
CCCGCGTGGACATCTGCGACCGCGCGGCACTCGACGCGCTGTTGGCTGAGCACCGGCCGCGCGCGATCCTGCATTTCGCG
GCGGAAAGTCACGTCGATCGCTCGATTCACGGTCCGGCCGAGTTCGTTCAGACCAACGTCGTCGGCACCTTCACGCTGCT
CGAGGCCGCGCGCCAGTACTGGAGCGCGTTACCCGACGCGGAGCAGGCCGGCTTCCGGTTCCTGCACGTGTCGACCGACG
AAGTGTTCGGTTCGCTGTCCGCGACGGATCCGCAGTTCTCTGAAACGACACCGTACGCACCCAACAGCCCGTACTCGGCG
ACGAAGGCGGGCTCCGATCACCTGGTGCGCGCGTACCACCATACCTATGGCCTGCCGACGCTCACGACCAACTGCTCGAA
CAACTACGGCCCGTACCAGTTCCCCGAAAAGCTGATTCCGCTGATGATTGCGAATGCACTCGCGGGCAAGCCGTTGCCGG
TCTACGGCGATGGCCAGAACGTGCGCGACTGGCTGTATGTCGGCGATCACTGCAGCGCGATTCGCGAAGTGCTCGCACGC
GGCACGCCGGGCGAGACCTACAACGTCGGCGGCTGGAACGAAATGACGAACCTCGACGTCGTGCATACGCTGTGCGATCT
GCTCGACGATGCTCGACCCAGGGCACAAGGCACGTATCGCGACCAGATTACGTACGTGAAGGATCGTCCGGGGCACGATC
GCCGTTACGCGATCGATGCGCGCAAGCTCGAGCGCGAACTCGGCTGGAAGCCGGACGAGACTTTCGCAACCGGCCTCGCG
AAGACGGTCTCCTGGTATCTCGACAACCAGGCGTGGTCGGACGAGGTGGCTTCGGGCGAATACCGCAAGTGGGTCGAGAC
CAATTACGCAGCGCGCGTTTGA

Upstream 100 bases:

>100_bases
TTAGAATGCCCGCCTTGGGGGGACGTGCGCGTCATCGTCGCGTCCGGAGTTCGTGGCGGCTCGTGCTTGAGACACGCCAT
GCATGACTAGAGGGAGCCTC

Downstream 100 bases:

>100_bases
CAGGAGAGGCGATGGCACGCAAAGGCATCATTCTCGCCGGCGGTTCAGGCACACGGCTCTATCCGATCACGCATGCAGTA
TCGAAGCAACTGCTGCCGGT

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVIDWLCQSDEAVLNVDKLTYAGNLRTLQSLNGSPKHVFARVDICDRAALDALLAEHRPRAILHFA
AESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWSALPDAEQAGFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GTPGETYNVGGWNEMTNLDVVHTLCDLLDDARPRAQGTYRDQITYVKDRPGHDRRYAIDARKLERELGWKPDETFATGLA
KTVSWYLDNQAWSDEVASGEYRKWVETNYAARV

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVIDWLCQSDEAVLNVDKLTYAGNLRTLQSLNGSPKHVFARVDICDRAALDALLAEHRPRAILHFA
AESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWSALPDAEQAGFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GTPGETYNVGGWNEMTNLDVVHTLCDLLDDARPRAQGTYRDQITYVKDRPGHDRRYAIDARKLERELGWKPDETFATGLA
KTVSWYLDNQAWSDEVASGEYRKWVETNYAARV
>Mature_353_residues
MILVTGGAGFIGANFVIDWLCQSDEAVLNVDKLTYAGNLRTLQSLNGSPKHVFARVDICDRAALDALLAEHRPRAILHFA
AESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWSALPDAEQAGFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYHHTYGLPTLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GTPGETYNVGGWNEMTNLDVVHTLCDLLDDARPRAQGTYRDQITYVKDRPGHDRRYAIDARKLERELGWKPDETFATGLA
KTVSWYLDNQAWSDEVASGEYRKWVETNYAARV

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=34.6504559270517, Blast_Score=219, Evalue=2e-57,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=23.8670694864048, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI56237023, Length=298, Percent_Identity=26.1744966442953, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI56118217, Length=298, Percent_Identity=26.1744966442953, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI189083684, Length=298, Percent_Identity=26.1744966442953, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4504031, Length=335, Percent_Identity=24.1791044776119, Blast_Score=69, Evalue=7e-12,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=60.1671309192201, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=61.9186046511628, Blast_Score=447, Evalue=1e-127,
Organism=Escherichia coli, GI1786974, Length=352, Percent_Identity=27.8409090909091, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788366, Length=361, Percent_Identity=24.9307479224377, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=332, Percent_Identity=36.144578313253, Blast_Score=207, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI115532424, Length=335, Percent_Identity=32.2388059701493, Blast_Score=173, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=368, Percent_Identity=26.3586956521739, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=370, Percent_Identity=26.2162162162162, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=22.3564954682779, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507723, Length=344, Percent_Identity=24.1279069767442, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI133901786, Length=275, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901790, Length=275, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI133901788, Length=275, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539422, Length=275, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17539424, Length=275, Percent_Identity=25.4545454545455, Blast_Score=72, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=298, Percent_Identity=26.8456375838926, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19923002, Length=364, Percent_Identity=28.2967032967033, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356223, Length=339, Percent_Identity=24.7787610619469, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24158427, Length=335, Percent_Identity=25.6716417910448, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39242; Mature: 39242

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVIDWLCQSDEAVLNVDKLTYAGNLRTLQSLNGSPKHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHEEECCCHHHHHHCCCCCCEEEEEECHHH
RAALDALLAEHRPRAILHFAAESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWSALPDA
HHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCC
EQAGFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPT
HHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GTPGETYNVGGWNEMTNLDVVHTLCDLLDDARPRAQGTYRDQITYVKDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCEEEEECCCCCCCEEEHHH
RKLERELGWKPDETFATGLAKTVSWYLDNQAWSDEVASGEYRKWVETNYAARV
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure
MILVTGGAGFIGANFVIDWLCQSDEAVLNVDKLTYAGNLRTLQSLNGSPKHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCCEEEEHHEEECCCHHHHHHCCCCCCEEEEEECHHH
RAALDALLAEHRPRAILHFAAESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWSALPDA
HHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCC
EQAGFRFLHVSTDEVFGSLSATDPQFSETTPYAPNSPYSATKAGSDHLVRAYHHTYGLPT
HHCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GTPGETYNVGGWNEMTNLDVVHTLCDLLDDARPRAQGTYRDQITYVKDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHCEEEEECCCCCCCEEEHHH
RKLERELGWKPDETFATGLAKTVSWYLDNQAWSDEVASGEYRKWVETNYAARV
HHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]